P70011 (NDKA2_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Nucleoside diphosphate kinase A2 Short name=NDK A2 Short name=NDP kinase A2 EC=2.7.4.6 Alternative name(s): NM23/nucleoside diphosphate kinase A2
Organism	Xenopus laevis (African clawed frog)
Taxonomic identifier	8355 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
Cofactor	Magnesium By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 154	154	Nucleoside diphosphate kinase A2		PRO_0000137121
Sites
	Active site	119	1	Pros-phosphohistidine intermediate By similarity
	Binding site	13	1	ATP By similarity
	Binding site	61	1	ATP By similarity
	Binding site	89	1	ATP By similarity
	Binding site	95	1	ATP By similarity
	Binding site	106	1	ATP By similarity
	Binding site	116	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P70011 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 820D35C65F7013EC	FASTA	154	17,502
	10 20 30 40 50 60 MAANKERTFI AIKPDGVQRG LMGDIIKRFE QKGFRLVAMK FLQASQDLLR QHYIDLKDRP 70 80 90 100 110 120 FYPGLVEYMN SGPVLAMVWE GLNVVKTGRV MLGETNPADS KPGTIRGDLC IQVGRNIIHG 130 140 150 SDSVDSANKE IALWFKDEEL VEYKSCAYEW VYEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Several genes encode the metastasis suppressor NM23/nucleoside diphosphate kinase of Xenopus laevis." Ouatas T., Abdallah B., Gasmi L., Mazabraud A. Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X97902 mRNA. Translation: CAA66476.1. BC087324 mRNA. Translation: AAH87324.1.
RefSeq	NP_001084144.1. NM_001090675.1.
UniGene	Xl.1257.
3D structure databases
ProteinModelPortal	P70011.
SMR	P70011. Positions 3-153.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	399333.
KEGG	xla:399333.
Organism-specific databases
CTD	399333.
Xenbase	XB-GENE-6254044. nme2.
Phylogenomic databases
HOVERGEN	HBG000423.
Family and domain databases
InterPro	IPR001564. Nucleoside_diP_kinase. IPR023005. Nucleoside_diP_kinase_AS. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
KO	K00940.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
SMART	SM00562. NDK. 1 hit. [Graphical view]
SUPFAM	SSF54919. NDK. 1 hit.
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDKA2_XENLA

Accession

Primary (citable) accession number: P70011
Secondary accession number(s): Q5PQ80

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 1, 1997
Last modified:	November 16, 2011
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program