Reviewed, UniProtKB/Swiss-Prot P70010 (NDKA1_XENLA)

Names and origin

Protein names	Recommended name:     Nucleoside diphosphate kinase A1       Short name=NDP kinase A1       Short name=NDK A1     EC=2.7.4.6 Alternative name(s):     NM23/nucleoside diphosphate kinase A1
Organism	Xenopus laevis (African clawed frog)
Taxonomic identifier	8355 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
Cofactor	Magnesium By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 154	154	Nucleoside diphosphate kinase A1		PRO_0000137120
Sites
	Active site	119	1	Pros-phosphohistidine intermediate By similarity
	Binding site	13	1	ATP By similarity
	Binding site	61	1	ATP By similarity
	Binding site	89	1	ATP By similarity
	Binding site	95	1	ATP By similarity
	Binding site	106	1	ATP By similarity
	Binding site	116	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P70010-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: DDE3A5621ADB6333	FASTA	154	17,489
	10 20 30 40 50 60 MAANKERTFI AIKPDGVQRG LMGDIIKRFE QKGFRLVAMK FQQASQDLLR QHYIDLKDRP 70 80 90 100 110 120 FYPGLVEYMS SGPVLAMVWE GLNVVKTGRV MLGETNPADS KPGTIRGDFC IQVGRNIIHG 130 140 150 SDSVESANKE IALWFKDEEL VENKSCAYEW VYEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Several genes encode the metastasis suppressor NM23/nucleoside diphosphate kinase of Xenopus laevis." Ouatas T., Abdallah B., Gasmi L., Mazabraud A. Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X97900 mRNA. Translation: CAA66474.1. BC079795 mRNA. Translation: AAH79795.1.
UniGene	Xl.2830
3D structure databases
HSSP	HSSP built from PDB template 1BE4 based on UniProtKB P52175.
SMR	P70010. Positions 3-153.
ModBase	Search...
Proteomic databases
PRIDE	P70010.
Organism-specific databases
Xenbase	XB-FEAT-923232. nme2.
Phylogenomic databases
HOVERGEN	P70010.
Enzyme and pathway databases
BRENDA	2.7.4.6. 648.
Family and domain databases
InterPro	IPR001564. Nuc_diP_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
ProDom	PD001018. NDK. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00562. NDK. 1 hit. [Graphical view]
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDKA1_XENLA

Accession

Primary (citable) accession number: P70010
Secondary accession number(s): Q68FI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project