Reviewed, UniProtKB/Swiss-Prot P69953 (BIOD2_YERPE)

Names and origin

Protein names

Recommended name:
    Probable dethiobiotin synthetase 2
      Short name=Dethiobiotin synthase 2
      Short name=DTB synthetase 2
      Short name=DTBS 2
    EC=6.3.3.3

Gene names

Name:	bioD2
Synonyms:	bioD
Ordered Locus Names:	YPO2269, y2111, YP_2065

Organism

Yersinia pestis [Complete proteome] [HAMAP]

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP cobalamin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cobyrinic acid a,c-diamide synthase activity Inferred from electronic annotation. Source: InterPro dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Probable dethiobiotin synthetase 2 HAMAP MF_00336		PRO_0000188003
Regions
	Nucleotide binding	9 – 17	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P69953-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 4E17C7A0BC32EF85	FASTA	222	24,192
	10 20 30 40 50 60 MLTRLFVTGT DTAVGKTVVS RALLQALSQN GRTAVGYKPV ATESKETSEG LRNQDALILQ 70 80 90 100 110 120 ASSSIELNYQ EVNPYPLQGD VIHACTDTLI NYEKMTEGLQ CLSAKADTVI VEGCGGWKVM 130 140 150 160 170 180 MNDQRFYSDW VVQEQLPVIL VVGIKLGCIN HALLTAQAII NDGLPLLGWV ANRINPGLAH 190 200 210 220 YAETIAMLRD RLAAPQLGQL PYLPRPEEKP LAKYLDLTAI SG

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed: 11586360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed: 12142430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM5 / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed: 15368893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL590842 Genomic DNA. Translation: CAL20896.1. AE009952 Genomic DNA. Translation: AAM85674.1. Different initiation. AE017042 Genomic DNA. Translation: AAS62277.1. Different initiation.
PIR	AE0276.
RefSeq	NP_669423.1. NP_993400.1. YP_002347235.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1147058. 1175100. 2764013.
GenomeReviews	Gene locus y2111 in contig AE009952_GR. Gene locus YP_2065 in contig AE017042_GR. Gene locus YPO2269 in contig AL590842_GR.
KEGG	ype:YPO2269. ypk:y2111. ypm:YP_2065.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P69953.
OMA	ETPEGMR.
Enzyme and pathway databases
BioCyc	YPES187410:Y2111-MON. YPES214092:YPO2269-MON. YPES229193:YP2065-MON.
BRENDA	6.3.3.3. 142588.
Family and domain databases
HAMAP	MF_00336. [Tree]
InterPro	IPR004472. BioD_synth. IPR002586. CbiA_P_synth. [Graphical view]
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD2_YERPE

Accession

Primary (citable) accession number: P69953
Secondary accession number(s): Q0WEQ3, Q66AD9, Q9AGD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)