P69942 (GLNE_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate-ammonia-ligase adenylyltransferase
EC=2.7.7.42

Alternative name(s):
Glutamine-synthetase adenylyltransferase
Short name=ATase
[Glutamate--ammonia-ligase] adenylyltransferase

Gene names

Name:	glnE
Ordered Locus Names:	Rv2221c, MT2279
ORF Names:	MTCY190.32c, MTCY427.02c

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	994 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Adenylation and deadenylation of glutamate--ammonia ligase By similarity. HAMAP MF_00802
Catalytic activity	ATP + [L-glutamate:ammonia ligase (ADP-forming)] = diphosphate + adenylyl-[L-glutamate:ammonia ligase (ADP-forming)]. HAMAP MF_00802
Miscellaneous	Was identified as a high-confidence drug target. HAMAP MF_00802
Sequence similarities	Belongs to the glnE family.

Ontologies

Keywords
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	growth Inferred from mutant phenotype. Source: MTBBASE regulation of glutamine family amino acid metabolic process Inferred from mutant phenotype. Source: MTBBASE response to ammonium ion Inferred from expression pattern. Source: MTBBASE
Cellular component	plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [glutamate-ammonia-ligase] adenylyltransferase activity Inferred from mutant phenotype. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 994	994	Glutamate-ammonia-ligase adenylyltransferase HAMAP MF_00802		PRO_0000209257
Regions
	Region	150 – 341	192	GlnE 1 HAMAP MF_00802
	Region	645 – 852	208	GlnE 2 HAMAP MF_00802
Experimental info
	Sequence conflict	676	1	T → S in AAK46564. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P69942 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 8E5EA5D63CCE25E0	FASTA	994	109,138
	10 20 30 40 50 60 MVVTKLATQR PKLPSVGRLG LVDPPAGERL AQLGWDRHED QAHVDLLWSL SRAPDADAAL 70 80 90 100 110 120 RALIRLSENP DTGWDELNAA LLRERSLRGR LFSVLGSSLA LGDHLVAHPQ SWKLLRGKVT 130 140 150 160 170 180 LPSHDQLQRS FVECVEESEG MPGSLVHRLR TQYRDYVLML AALDLAATVE DEPVLPFTVV 190 200 210 220 230 240 AARLADAADA ALAAALRVAE ASVCGEHPPP RLAVIAMGKC GARELNYVSD VDVIFVAERS 250 260 270 280 290 300 DPRNARVASE MMRVASAAFF EVDAALRPEG RNGELVRTLE SHIAYYQRWA KTWEFQALLK 310 320 330 340 350 360 ARPVVGDAEL GERYLTALMP MVWRACERED FVVEVQAMRR RVEQLVPADV RGRELKLGSG 370 380 390 400 410 420 GLRDVEFAVQ LLQLVHARSD ESLRVASTVD ALAALGEGGY IGREDAANMT ASYEFLRLLE 430 440 450 460 470 480 HRLQLQRLKR THLLPDPEDE EAVRWLARAA HIRPDGRNDA AGVLREELKK QNVRVSKLHT 490 500 510 520 530 540 KLFYQPLLES IGPTGLEIAH GMTLEAAGRR LAALGYEGPQ TALKHMSALV NQSGRRGRVQ 550 560 570 580 590 600 SVLLPRLLDW MSYAPDPDGG LLAYRRLSEA LATESWYLAT LRDKPAVAKR LMHVLGTSAY 610 620 630 640 650 660 VPDLLMRAPR VIQQYEDGPA GPKLLETEPA AVARALIASA SRYPDPERAI AGARTLRRRE 670 680 690 700 710 720 LARIGSADLL GLLEVTEVCR ALTSVWVAVL QAALDVMIRA SLPDDDRAPA AIAVIGMGRL 730 740 750 760 770 780 GGAELGYGSD ADVMFVCEPA TGVDDARAVK WSTSIAERVR ALLGTPSVDP PLELDANLRP 790 800 810 820 830 840 EGRNGPLVRT LGSYAAYYEQ WAQPWEIQAL LRAHAVAGDA ELGQRFLRMV DKTRYPPDGV 850 860 870 880 890 900 SADSVREIRR IKARIESERL PRGADPNTHT KLGRGGLADI EWTVQLLQLQ HAHQVPALHN 910 920 930 940 950 960 TSTLQSLDVI AAADLVPAAD VELLRQAWLT ATRARNALVL VRGKPTDQLP GPGRQLNAVA 970 980 990 VAAGWRNDDG GEFLDNYLRV TRRAKAVVRK VFGS

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842579 Genomic DNA. Translation: CAA94664.1. AE000516 Genomic DNA. Translation: AAK46564.1.
PIR	A70776.
RefSeq	NP_216737.1. NC_000962.2. NP_336750.1. NC_002755.2.
3D structure databases
ProteinModelPortal	P69942.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000000261; EBMYCP00000000261; EBMYCG00000000261. EBMYCT00000070205; EBMYCP00000068264; EBMYCG00000070200.
GeneID	887995. 924147.
GenomeReviews	Gene locus MT2279 in contig AE000516_GR. Gene locus Rv2221c in contig AL123456_GR.
KEGG	mtc:MT2279. mtu:Rv2221c.
PATRIC	18126758. VBIMycTub22151_2491.
TIGR	MT2279.
Organism-specific databases
TubercuList	Rv2221c.
Phylogenomic databases
GeneTree	EBGT00050000016992.
HOGENOM	HBG292568.
OMA	VEHRIQM.
PhylomeDB	P69942.
ProtClustDB	PRK14109.
Family and domain databases
HAMAP	MF_00802. GlnE. [Tree]
InterPro	IPR023057. GlnE. IPR005190. GlnE_rpt_dom. IPR013546. PII_UdlTrfase/GS_AdlTrfase. [Graphical view]
KO	K00982.
Pfam	PF08335. GlnD_UR_UTase. 2 hits. PF03710. GlnE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLNE_MYCTU

Accession

Primary (citable) accession number: P69942
Secondary accession number(s): Q10379

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	January 4, 2005
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program