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Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:Mb2245c
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002092541 – 994Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST994

Structurei

3D structure databases

ProteinModelPortaliP69941.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 487Adenylyl removaseUniRule annotationAdd BLAST487
Regioni492 – 994Adenylyl transferaseUniRule annotationAdd BLAST503

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000243040.
KOiK00982.
OMAiEFMVQYA.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.

Sequencei

Sequence statusi: Complete.

P69941-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVTKLATQR PKLPSVGRLG LVDPPAGERL AQLGWDRHED QAHVDLLWSL
60 70 80 90 100
SRAPDADAAL RALIRLSENP DTGWDELNAA LLRERSLRGR LFSVLGSSLA
110 120 130 140 150
LGDHLVAHPQ SWKLLRGKVT LPSHDQLQRS FVECVEESEG MPGSLVHRLR
160 170 180 190 200
TQYRDYVLML AALDLAATVE DEPVLPFTVV AARLADAADA ALAAALRVAE
210 220 230 240 250
ASVCGEHPPP RLAVIAMGKC GARELNYVSD VDVIFVAERS DPRNARVASE
260 270 280 290 300
MMRVASAAFF EVDAALRPEG RNGELVRTLE SHIAYYQRWA KTWEFQALLK
310 320 330 340 350
ARPVVGDAEL GERYLTALMP MVWRACERED FVVEVQAMRR RVEQLVPADV
360 370 380 390 400
RGRELKLGSG GLRDVEFAVQ LLQLVHARSD ESLRVASTVD ALAALGEGGY
410 420 430 440 450
IGREDAANMT ASYEFLRLLE HRLQLQRLKR THLLPDPEDE EAVRWLARAA
460 470 480 490 500
HIRPDGRNDA AGVLREELKK QNVRVSKLHT KLFYQPLLES IGPTGLEIAH
510 520 530 540 550
GMTLEAAGRR LAALGYEGPQ TALKHMSALV NQSGRRGRVQ SVLLPRLLDW
560 570 580 590 600
MSYAPDPDGG LLAYRRLSEA LATESWYLAT LRDKPAVAKR LMHVLGTSAY
610 620 630 640 650
VPDLLMRAPR VIQQYEDGPA GPKLLETEPA AVARALIASA SRYPDPERAI
660 670 680 690 700
AGARTLRRRE LARIGSADLL GLLEVTEVCR ALTSVWVAVL QAALDVMIRA
710 720 730 740 750
SLPDDDRAPA AIAVIGMGRL GGAELGYGSD ADVMFVCEPA TGVDDARAVK
760 770 780 790 800
WSTSIAERVR ALLGTPSVDP PLELDANLRP EGRNGPLVRT LGSYAAYYEQ
810 820 830 840 850
WAQPWEIQAL LRAHAVAGDA ELGQRFLRMV DKTRYPPDGV SADSVREIRR
860 870 880 890 900
IKARIESERL PRGADPNTHT KLGRGGLADI EWTVQLLQLQ HAHQVPALHN
910 920 930 940 950
TSTLQSLDVI AAADLVPAAD VELLRQAWLT ATRARNALVL VRGKPTDQLP
960 970 980 990
GPGRQLNAVA VAAGWRNDDG GEFLDNYLRV TRRAKAVVRK VFGS
Length:994
Mass (Da):109,138
Last modified:January 4, 2005 - v1
Checksum:i8E5EA5D63CCE25E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO43499.1.
RefSeqiNP_855894.1. NC_002945.3.
WP_003411478.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCDO43499; CDO43499; Mb2245c.
GeneIDi1091370.
KEGGimbo:Mb2245c.
PATRICi18006632. VBIMycBov88188_2462.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO43499.1.
RefSeqiNP_855894.1. NC_002945.3.
WP_003411478.1. NC_002945.3.

3D structure databases

ProteinModelPortaliP69941.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCDO43499; CDO43499; Mb2245c.
GeneIDi1091370.
KEGGimbo:Mb2245c.
PATRICi18006632. VBIMycBov88188_2462.

Phylogenomic databases

HOGENOMiHOG000243040.
KOiK00982.
OMAiEFMVQYA.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGLNE_MYCBO
AccessioniPrimary (citable) accession number: P69941
Secondary accession number(s): Q10379, X2BK61
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 4, 2005
Last modified: February 15, 2017
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.