Skip Header

Contribute Send feedback
Read comments (?) or add your own

P69925 (RIR2_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonucleoside-diphosphate reductase 1 subunit beta
EC=1.17.4.1
Alternative name(s):
Protein B2
Protein R2
Ribonucleotide reductase 1
Gene names
Name:nrdB
Synonyms:ftsB
Ordered Locus Names:Z3491, ECs3118
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length376 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2 contains the tyrosyl radical required for catalysis.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactor

Binds 2 iron ions per subunit.

Pathway

Genetic information processing; DNA replication.

Subunit structure

Tetramer of two alpha (R1) and two beta (R2) subunits. The B1 protein is a dimer of alpha subunits. A radical transfer pathway occurs between Tyr-123 of R2 and R1 By similarity.

Miscellaneous

E.coli produces two separate class I enzymes. This one is the functional enzyme during growth.

A substrate-binding catalytic site, located on R1, is formed only in the presence of the second subunit R2 By similarity.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase small chain family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 376375Ribonucleoside-diphosphate reductase 1 subunit beta
PRO_0000190477

Sites

Active site1231 By similarity
Metal binding851Iron 1 By similarity
Metal binding1161Iron 1 By similarity
Metal binding1161Iron 2 By similarity
Metal binding1191Iron 1 By similarity
Metal binding2051Iron 2 By similarity
Metal binding2391Iron 2 By similarity
Metal binding2421Iron 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
P69925 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: BF2D9A49B643E84A

FASTA37643,517
        10         20         30         40         50         60 
MAYTTFSQTK NDQLKEPMFF GQPVNVARYD QQKYDIFEKL IEKQLSFFWR PEEVDVSRDR 

        70         80         90        100        110        120 
IDYQALPEHE KHIFISNLKY QTLLDSIQGR SPNVALLPLI SIPELETWVE TWAFSETIHS 

       130        140        150        160        170        180 
RSYTHIIRNI VNDPSVVFDD IVTNEQIQKR AEGISSYYDE LIEMTSYWHL LGEGTHTVNG 

       190        200        210        220        230        240 
KTVTVSLREL KKKLYLCLMS VNALEAIRFY VSFACSFAFA ERELMEGNAK IIRLIARDEA 

       250        260        270        280        290        300 
LHLTGTQHML NLLRSGADDP EMAEIAEECK QECYDLFVQA AQQEKDWADY LFRDGSMIGL 

       310        320        330        340        350        360 
NKDILCQYVE YITNIRMQAV GLDLPFQTRS NPIPWINTWL VSDNVQVAPQ EVEVSSYLVG 

       370 
QIDSEVDTDD LSNFQL

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE005174 Genomic DNA. Translation: AAG57364.1.
BA000007 Genomic DNA. Translation: BAB36541.1.
PIRF91018.
H85862.
RefSeqNP_288809.1. NC_002655.2.
NP_311145.1. NC_002695.1.

3D structure databases

ProteinModelPortalP69925.
SMRP69925. Positions 2-376.
ModBaseSearch...

Protein-protein interaction databases

MINTMINT-1233701.
STRING155864.Z3491.

Proteomic databases

PRIDEP69925.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG57364; AAG57364; Z3491.
BAB36541; BAB36541; BAB36541.
GeneID916826.
957218.
KEGGece:Z3491.
ecs:ECs3118.
PATRIC18355620. VBIEscCol44059_3011.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0208.
HOGENOMHOG000278087.
KOK00526.
OMALEPMFLG.
ProtClustDBPRK09101.

Enzyme and pathway databases

BioCycECOL386585:GJFA-3070-MONOMER.
UniPathwayUPA00326.

Family and domain databases

Gene3D1.10.620.20. 1 hit.
InterProIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERPTHR23409. PTHR23409. 1 hit.
PfamPF00268. Ribonuc_red_sm. 2 hits.
[Graphical view]
SUPFAMSSF47240. Ferritin/RR_like. 1 hit.
PROSITEPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRIR2_ECO57
AccessionPrimary (citable) accession number: P69925
Secondary accession number(s): P00453
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 1, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents