Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P69925

- RIR2_ECO57

UniProt

P69925 - RIR2_ECO57

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Ribonucleoside-diphosphate reductase 1 subunit beta

Gene

nrdB

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2 contains the tyrosyl radical required for catalysis.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Binds 2 iron ions per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi85 – 851Iron 1PROSITE-ProRule annotation
Metal bindingi116 – 1161Iron 1PROSITE-ProRule annotation
Metal bindingi116 – 1161Iron 2By similarity
Metal bindingi119 – 1191Iron 1PROSITE-ProRule annotation
Active sitei123 – 1231PROSITE-ProRule annotation
Metal bindingi205 – 2051Iron 2By similarity
Metal bindingi239 – 2391Iron 2By similarity
Metal bindingi242 – 2421Iron 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
  2. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-3070-MONOMER.
ECOO157:NRDB-MONOMER.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase 1 subunit beta (EC:1.17.4.1)
Alternative name(s):
Protein B2
Protein R2
Ribonucleotide reductase 1
Gene namesi
Name:nrdB
Synonyms:ftsB
Ordered Locus Names:Z3491, ECs3118
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 376375Ribonucleoside-diphosphate reductase 1 subunit betaPRO_0000190477Add
BLAST

Proteomic databases

PRIDEiP69925.

Interactioni

Subunit structurei

Tetramer of two alpha (R1) and two beta (R2) subunits. The B1 protein is a dimer of alpha subunits. A radical transfer pathway occurs between Tyr-123 of R2 and R1 (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-1233701.
STRINGi155864.Z3491.

Structurei

3D structure databases

ProteinModelPortaliP69925.
SMRiP69925. Positions 2-376.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0208.
HOGENOMiHOG000278087.
KOiK00526.
OMAiWIETWAF.
OrthoDBiEOG6J48J7.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 2 hits.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69925-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAYTTFSQTK NDQLKEPMFF GQPVNVARYD QQKYDIFEKL IEKQLSFFWR
60 70 80 90 100
PEEVDVSRDR IDYQALPEHE KHIFISNLKY QTLLDSIQGR SPNVALLPLI
110 120 130 140 150
SIPELETWVE TWAFSETIHS RSYTHIIRNI VNDPSVVFDD IVTNEQIQKR
160 170 180 190 200
AEGISSYYDE LIEMTSYWHL LGEGTHTVNG KTVTVSLREL KKKLYLCLMS
210 220 230 240 250
VNALEAIRFY VSFACSFAFA ERELMEGNAK IIRLIARDEA LHLTGTQHML
260 270 280 290 300
NLLRSGADDP EMAEIAEECK QECYDLFVQA AQQEKDWADY LFRDGSMIGL
310 320 330 340 350
NKDILCQYVE YITNIRMQAV GLDLPFQTRS NPIPWINTWL VSDNVQVAPQ
360 370
EVEVSSYLVG QIDSEVDTDD LSNFQL
Length:376
Mass (Da):43,517
Last modified:January 23, 2007 - v2
Checksum:iBF2D9A49B643E84A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG57364.1.
BA000007 Genomic DNA. Translation: BAB36541.1.
PIRiF91018.
H85862.
RefSeqiNP_288809.1. NC_002655.2.
NP_311145.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG57364; AAG57364; Z3491.
BAB36541; BAB36541; BAB36541.
GeneIDi916826.
957218.
KEGGiece:Z3491.
ecs:ECs3118.
PATRICi18355620. VBIEscCol44059_3011.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG57364.1 .
BA000007 Genomic DNA. Translation: BAB36541.1 .
PIRi F91018.
H85862.
RefSeqi NP_288809.1. NC_002655.2.
NP_311145.1. NC_002695.1.

3D structure databases

ProteinModelPortali P69925.
SMRi P69925. Positions 2-376.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-1233701.
STRINGi 155864.Z3491.

Proteomic databases

PRIDEi P69925.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG57364 ; AAG57364 ; Z3491 .
BAB36541 ; BAB36541 ; BAB36541 .
GeneIDi 916826.
957218.
KEGGi ece:Z3491.
ecs:ECs3118.
PATRICi 18355620. VBIEscCol44059_3011.

Phylogenomic databases

eggNOGi COG0208.
HOGENOMi HOG000278087.
KOi K00526.
OMAi WIETWAF.
OrthoDBi EOG6J48J7.

Enzyme and pathway databases

UniPathwayi UPA00326 .
BioCyci ECOL386585:GJFA-3070-MONOMER.
ECOO157:NRDB-MONOMER.

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view ]
PANTHERi PTHR23409. PTHR23409. 1 hit.
Pfami PF00268. Ribonuc_red_sm. 2 hits.
[Graphical view ]
SUPFAMi SSF47240. SSF47240. 1 hit.
PROSITEi PS00368. RIBORED_SMALL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiRIR2_ECO57
AccessioniPrimary (citable) accession number: P69925
Secondary accession number(s): P00453
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

E.coli produces two separate class I enzymes. This one is the functional enzyme during growth.
A substrate-binding catalytic site, located on R1, is formed only in the presence of the second subunit R2.By similarity

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3