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Protein

Spermidine/putrescine import ATP-binding protein PotA

Gene

potA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system.UniRule annotation3 Publications

Catalytic activityi

ATP + H2O + polyamine(Out) = ADP + phosphate + polyamine(In).UniRule annotation

Enzyme regulationi

Strongly inhibited by carbonyl cyanide m-chlorophenylhydrazone (CCCP), and by spermidine via its interaction with the C-terminus. Also inhibited by N-ethylmaleimide and p-chloromercuribenzoic acid. Partially inhibited by KCN.4 Publications

Kineticsi

  1. KM=385 µM for ATP2 Publications
  1. Vmax=400 nmol/min/mg enzyme2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi50 – 57ATPUniRule annotation8

GO - Molecular functioni

  • ATP binding Source: EcoCyc
  • polyamine-transporting ATPase activity Source: EcoCyc
  • putrescine-importing ATPase activity Source: InterPro
  • spermidine-importing ATPase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:POTA-MONOMER.
ECOL316407:JW1112-MONOMER.
MetaCyc:POTA-MONOMER.

Protein family/group databases

TCDBi3.A.1.11.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Spermidine/putrescine import ATP-binding protein PotAUniRule annotation (EC:3.6.3.31UniRule annotation)
Gene namesi
Name:potAUniRule annotation
Ordered Locus Names:b1126, JW1112
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10749. potA.

Subcellular locationi

  • Cell inner membrane UniRule annotation2 Publications; Peripheral membrane protein UniRule annotation2 Publications

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi26C → A: Lower ATPase activity and transport efficiency. 1 Publication1
Mutagenesisi27F → L: Lower ATPase activity and transport efficiency. 1 Publication1
Mutagenesisi45F → L: Lower ATPase activity and transport efficiency. 1 Publication1
Mutagenesisi54C → T: Loss of ATPase activity and transport. 1 Publication1
Mutagenesisi60L → F: Lower ATPase activity and transport efficiency. 1 Publication1
Mutagenesisi76L → P: Lower ATPase activity and transport efficiency. 1 Publication1
Mutagenesisi135V → M: Loss of ATPase activity and transport. 2 Publications1
Mutagenesisi172D → N: Loss of ATPase activity and transport. 1 Publication1
Mutagenesisi276C → A: Lower ATPase activity and transport efficiency. 1 Publication1
Mutagenesisi297E → K or D: Lower ATPase activity and transport efficiency. 1 Publication1
Mutagenesisi297E → Q: Loss of ATPase activity and transport. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000927461 – 378Spermidine/putrescine import ATP-binding protein PotAAdd BLAST378

Post-translational modificationi

The N-terminus is blocked.Curated

Proteomic databases

EPDiP69874.
PaxDbiP69874.
PRIDEiP69874.

Expressioni

Inductioni

Transcription is inhibited by PotD precursor, probably only when excess amounts of PotD are produced.1 Publication

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD).UniRule annotation

Protein-protein interaction databases

BioGridi4261674. 3 interactors.
IntActiP69874. 2 interactors.
STRINGi511145.b1126.

Structurei

3D structure databases

ProteinModelPortaliP69874.
SMRiP69874.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 248ABC transporterUniRule annotationAdd BLAST231

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Spermidine/putrescine importer (TC 3.A.1.11.1) family. [View classification]UniRule annotation
Contains 1 ABC transporter domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C53. Bacteria.
COG3842. LUCA.
InParanoidiP69874.
KOiK11072.
OMAiIGYIRER.
PhylomeDBiP69874.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR017879. ABC_Sperm/Put_ATP-bd_PotA.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR008995. Mo/tungstate-bd_C_term_dom.
IPR027417. P-loop_NTPase.
IPR005893. Sp_pt_ABC_ATP-bd.
IPR013611. Transp-assoc_OB_typ2.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF08402. TOBE_2. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50331. SSF50331. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01187. potA. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51305. POTA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69874-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQSKKLNKQ PSSLSPLVQL AGIRKCFDGK EVIPQLDLTI NNGEFLTLLG
60 70 80 90 100
PSGCGKTTVL RLIAGLETVD SGRIMLDNED ITHVPAENRY VNTVFQSYAL
110 120 130 140 150
FPHMTVFENV AFGLRMQKTP AAEITPRVME ALRMVQLETF AQRKPHQLSG
160 170 180 190 200
GQQQRVAIAR AVVNKPRLLL LDESLSALDY KLRKQMQNEL KALQRKLGIT
210 220 230 240 250
FVFVTHDQEE ALTMSDRIVV MRDGRIEQDG TPREIYEEPK NLFVAGFIGE
260 270 280 290 300
INMFNATVIE RLDEQRVRAN VEGRECNIYV NFAVEPGQKL HVLLRPEDLR
310 320 330 340 350
VEEINDDNHA EGLIGYVRER NYKGMTLESV VELENGKMVM VSEFFNEDDP
360 370
DFDHSLDQKM AINWVESWEV VLADEEHK
Length:378
Mass (Da):43,028
Last modified:December 21, 2004 - v1
Checksum:i14DCA99329A344F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64519 Genomic DNA. Translation: AAC37038.1.
U00096 Genomic DNA. Translation: AAC74210.1.
AP009048 Genomic DNA. Translation: BAA35946.1.
PIRiA40840.
RefSeqiNP_415644.1. NC_000913.3.
WP_000531594.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74210; AAC74210; b1126.
BAA35946; BAA35946; BAA35946.
GeneIDi946323.
KEGGiecj:JW1112.
eco:b1126.
PATRICi32117499. VBIEscCol129921_1172.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64519 Genomic DNA. Translation: AAC37038.1.
U00096 Genomic DNA. Translation: AAC74210.1.
AP009048 Genomic DNA. Translation: BAA35946.1.
PIRiA40840.
RefSeqiNP_415644.1. NC_000913.3.
WP_000531594.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP69874.
SMRiP69874.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261674. 3 interactors.
IntActiP69874. 2 interactors.
STRINGi511145.b1126.

Protein family/group databases

TCDBi3.A.1.11.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

EPDiP69874.
PaxDbiP69874.
PRIDEiP69874.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74210; AAC74210; b1126.
BAA35946; BAA35946; BAA35946.
GeneIDi946323.
KEGGiecj:JW1112.
eco:b1126.
PATRICi32117499. VBIEscCol129921_1172.

Organism-specific databases

EchoBASEiEB0742.
EcoGeneiEG10749. potA.

Phylogenomic databases

eggNOGiENOG4105C53. Bacteria.
COG3842. LUCA.
InParanoidiP69874.
KOiK11072.
OMAiIGYIRER.
PhylomeDBiP69874.

Enzyme and pathway databases

BioCyciEcoCyc:POTA-MONOMER.
ECOL316407:JW1112-MONOMER.
MetaCyc:POTA-MONOMER.

Miscellaneous databases

PROiP69874.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR017879. ABC_Sperm/Put_ATP-bd_PotA.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR008995. Mo/tungstate-bd_C_term_dom.
IPR027417. P-loop_NTPase.
IPR005893. Sp_pt_ABC_ATP-bd.
IPR013611. Transp-assoc_OB_typ2.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF08402. TOBE_2. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50331. SSF50331. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01187. potA. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51305. POTA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPOTA_ECOLI
AccessioniPrimary (citable) accession number: P69874
Secondary accession number(s): P23858
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.