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Protein

Spermidine/putrescine import ATP-binding protein PotA

Gene

potA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system.UniRule annotation3 Publications

Catalytic activityi

ATP + H2O + polyamine(Out) = ADP + phosphate + polyamine(In).UniRule annotation

Enzyme regulationi

Strongly inhibited by carbonyl cyanide m-chlorophenylhydrazone (CCCP), and by spermidine via its interaction with the C-terminus. Also inhibited by N-ethylmaleimide and p-chloromercuribenzoic acid. Partially inhibited by KCN.4 Publications

Kineticsi

  1. KM=385 µM for ATP2 Publications
  1. Vmax=400 nmol/min/mg enzyme2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi50 – 57ATPUniRule annotation8

GO - Molecular functioni

  • ATPase activity Source: EcoCyc
  • ATP binding Source: EcoCyc
  • nucleotide binding Source: EcoCyc
  • polyamine-transporting ATPase activity Source: EcoCyc
  • putrescine-importing ATPase activity Source: InterPro
  • spermidine-importing ATPase activity Source: EcoCyc

GO - Biological processi

  • putrescine transport Source: EcoCyc
  • spermidine transmembrane transport Source: EcoCyc

Keywordsi

Molecular functionHydrolase
Biological processTransport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:POTA-MONOMER
MetaCyc:POTA-MONOMER

Protein family/group databases

TCDBi3.A.1.11.1 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Spermidine/putrescine import ATP-binding protein PotAUniRule annotation (EC:3.6.3.31UniRule annotation)
Gene namesi
Name:potAUniRule annotation
Ordered Locus Names:b1126, JW1112
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10749 potA

Subcellular locationi

  • Cell inner membrane UniRule annotation2 Publications; Peripheral membrane protein UniRule annotation2 Publications

GO - Cellular componenti

  • ATP-binding cassette (ABC) transporter complex Source: EcoCyc
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi26C → A: Lower ATPase activity and transport efficiency. 1 Publication1
Mutagenesisi27F → L: Lower ATPase activity and transport efficiency. 1 Publication1
Mutagenesisi45F → L: Lower ATPase activity and transport efficiency. 1 Publication1
Mutagenesisi54C → T: Loss of ATPase activity and transport. 1 Publication1
Mutagenesisi60L → F: Lower ATPase activity and transport efficiency. 1 Publication1
Mutagenesisi76L → P: Lower ATPase activity and transport efficiency. 1 Publication1
Mutagenesisi135V → M: Loss of ATPase activity and transport. 2 Publications1
Mutagenesisi172D → N: Loss of ATPase activity and transport. 1 Publication1
Mutagenesisi276C → A: Lower ATPase activity and transport efficiency. 1 Publication1
Mutagenesisi297E → K or D: Lower ATPase activity and transport efficiency. 1 Publication1
Mutagenesisi297E → Q: Loss of ATPase activity and transport. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000927461 – 378Spermidine/putrescine import ATP-binding protein PotAAdd BLAST378

Post-translational modificationi

The N-terminus is blocked.Curated

Proteomic databases

EPDiP69874
PaxDbiP69874
PRIDEiP69874

Expressioni

Inductioni

Transcription is inhibited by PotD precursor, probably only when excess amounts of PotD are produced.1 Publication

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD).UniRule annotation

Protein-protein interaction databases

BioGridi4261674, 16 interactors
IntActiP69874, 2 interactors
STRINGi316385.ECDH10B_1198

Structurei

3D structure databases

ProteinModelPortaliP69874
SMRiP69874
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 248ABC transporterUniRule annotationAdd BLAST231

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Spermidine/putrescine importer (TC 3.A.1.11.1) family. [View classification]UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C53 Bacteria
COG3842 LUCA
InParanoidiP69874
KOiK11072
OMAiHVMRFNE
PhylomeDBiP69874

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR017879 ABC_Sperm/Put_ATP-bd_PotA
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR008995 Mo/tungstate-bd_C_term_dom
IPR027417 P-loop_NTPase
IPR005893 Sp_pt_ABC_ATP-bd
IPR013611 Transp-assoc_OB_typ2
PfamiView protein in Pfam
PF00005 ABC_tran, 1 hit
PF08402 TOBE_2, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF50331 SSF50331, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01187 potA, 1 hit
PROSITEiView protein in PROSITE
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 1 hit
PS51305 POTA, 1 hit

Sequencei

Sequence statusi: Complete.

P69874-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQSKKLNKQ PSSLSPLVQL AGIRKCFDGK EVIPQLDLTI NNGEFLTLLG
60 70 80 90 100
PSGCGKTTVL RLIAGLETVD SGRIMLDNED ITHVPAENRY VNTVFQSYAL
110 120 130 140 150
FPHMTVFENV AFGLRMQKTP AAEITPRVME ALRMVQLETF AQRKPHQLSG
160 170 180 190 200
GQQQRVAIAR AVVNKPRLLL LDESLSALDY KLRKQMQNEL KALQRKLGIT
210 220 230 240 250
FVFVTHDQEE ALTMSDRIVV MRDGRIEQDG TPREIYEEPK NLFVAGFIGE
260 270 280 290 300
INMFNATVIE RLDEQRVRAN VEGRECNIYV NFAVEPGQKL HVLLRPEDLR
310 320 330 340 350
VEEINDDNHA EGLIGYVRER NYKGMTLESV VELENGKMVM VSEFFNEDDP
360 370
DFDHSLDQKM AINWVESWEV VLADEEHK
Length:378
Mass (Da):43,028
Last modified:December 21, 2004 - v1
Checksum:i14DCA99329A344F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64519 Genomic DNA Translation: AAC37038.1
U00096 Genomic DNA Translation: AAC74210.1
AP009048 Genomic DNA Translation: BAA35946.1
PIRiA40840
RefSeqiNP_415644.1, NC_000913.3
WP_000531594.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74210; AAC74210; b1126
BAA35946; BAA35946; BAA35946
GeneIDi946323
KEGGiecj:JW1112
eco:b1126
PATRICifig|1411691.4.peg.1141

Similar proteinsi

Entry informationi

Entry nameiPOTA_ECOLI
AccessioniPrimary (citable) accession number: P69874
Secondary accession number(s): P23858
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: December 21, 2004
Last modified: March 28, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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