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Protein

Spermidine/putrescine import ATP-binding protein PotA

Gene

potA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system.UniRule annotation3 Publications

Catalytic activityi

ATP + H2O + polyamine(Out) = ADP + phosphate + polyamine(In).UniRule annotation

Enzyme regulationi

Strongly inhibited by carbonyl cyanide m-chlorophenylhydrazone (CCCP), and by spermidine via its interaction with the C-terminus. Also inhibited by N-ethylmaleimide and p-chloromercuribenzoic acid. Partially inhibited by KCN.4 Publications

Kineticsi

  1. KM=385 µM for ATP2 Publications
  1. Vmax=400 nmol/min/mg enzyme2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi50 – 578ATPUniRule annotation

GO - Molecular functioni

  • ATP binding Source: EcoCyc
  • polyamine-transporting ATPase activity Source: EcoCyc
  • putrescine-importing ATPase activity Source: InterPro
  • spermidine-importing ATPase activity Source: InterPro

GO - Biological processi

  • polyamine transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:POTA-MONOMER.
ECOL316407:JW1112-MONOMER.
MetaCyc:POTA-MONOMER.

Protein family/group databases

TCDBi3.A.1.11.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Spermidine/putrescine import ATP-binding protein PotAUniRule annotation (EC:3.6.3.31UniRule annotation)
Gene namesi
Name:potAUniRule annotation
Ordered Locus Names:b1126, JW1112
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10749. potA.

Subcellular locationi

  • Cell inner membrane UniRule annotation2 Publications; Peripheral membrane protein UniRule annotation2 Publications

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi26 – 261C → A: Lower ATPase activity and transport efficiency. 1 Publication
Mutagenesisi27 – 271F → L: Lower ATPase activity and transport efficiency. 1 Publication
Mutagenesisi45 – 451F → L: Lower ATPase activity and transport efficiency. 1 Publication
Mutagenesisi54 – 541C → T: Loss of ATPase activity and transport. 1 Publication
Mutagenesisi60 – 601L → F: Lower ATPase activity and transport efficiency. 1 Publication
Mutagenesisi76 – 761L → P: Lower ATPase activity and transport efficiency. 1 Publication
Mutagenesisi135 – 1351V → M: Loss of ATPase activity and transport. 2 Publications
Mutagenesisi172 – 1721D → N: Loss of ATPase activity and transport. 1 Publication
Mutagenesisi276 – 2761C → A: Lower ATPase activity and transport efficiency. 1 Publication
Mutagenesisi297 – 2971E → K or D: Lower ATPase activity and transport efficiency. 1 Publication
Mutagenesisi297 – 2971E → Q: Loss of ATPase activity and transport. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 378378Spermidine/putrescine import ATP-binding protein PotAPRO_0000092746Add
BLAST

Post-translational modificationi

The N-terminus is blocked.Curated

Proteomic databases

EPDiP69874.
PaxDbiP69874.
PRIDEiP69874.

Expressioni

Inductioni

Transcription is inhibited by PotD precursor, probably only when excess amounts of PotD are produced.1 Publication

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD).UniRule annotation

Protein-protein interaction databases

BioGridi4261674. 3 interactions.
IntActiP69874. 2 interactions.
STRINGi511145.b1126.

Structurei

3D structure databases

ProteinModelPortaliP69874.
SMRiP69874. Positions 14-378.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 248231ABC transporterUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Spermidine/putrescine importer (TC 3.A.1.11.1) family. [View classification]UniRule annotation
Contains 1 ABC transporter domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C53. Bacteria.
COG3842. LUCA.
InParanoidiP69874.
KOiK11072.
OMAiIGYIRER.
OrthoDBiEOG6T7N3V.
PhylomeDBiP69874.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR017879. ABC_Sperm/Put_ATP-bd_PotA.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR008995. Mo/tungstate-bd_C_term_dom.
IPR027417. P-loop_NTPase.
IPR005893. Sp_pt_ABC_ATP-bd.
IPR013611. Transp-assoc_OB_typ2.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF08402. TOBE_2. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50331. SSF50331. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01187. potA. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51305. POTA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69874-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQSKKLNKQ PSSLSPLVQL AGIRKCFDGK EVIPQLDLTI NNGEFLTLLG
60 70 80 90 100
PSGCGKTTVL RLIAGLETVD SGRIMLDNED ITHVPAENRY VNTVFQSYAL
110 120 130 140 150
FPHMTVFENV AFGLRMQKTP AAEITPRVME ALRMVQLETF AQRKPHQLSG
160 170 180 190 200
GQQQRVAIAR AVVNKPRLLL LDESLSALDY KLRKQMQNEL KALQRKLGIT
210 220 230 240 250
FVFVTHDQEE ALTMSDRIVV MRDGRIEQDG TPREIYEEPK NLFVAGFIGE
260 270 280 290 300
INMFNATVIE RLDEQRVRAN VEGRECNIYV NFAVEPGQKL HVLLRPEDLR
310 320 330 340 350
VEEINDDNHA EGLIGYVRER NYKGMTLESV VELENGKMVM VSEFFNEDDP
360 370
DFDHSLDQKM AINWVESWEV VLADEEHK
Length:378
Mass (Da):43,028
Last modified:December 21, 2004 - v1
Checksum:i14DCA99329A344F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64519 Genomic DNA. Translation: AAC37038.1.
U00096 Genomic DNA. Translation: AAC74210.1.
AP009048 Genomic DNA. Translation: BAA35946.1.
PIRiA40840.
RefSeqiNP_415644.1. NC_000913.3.
WP_000531594.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74210; AAC74210; b1126.
BAA35946; BAA35946; BAA35946.
GeneIDi946323.
KEGGiecj:JW1112.
eco:b1126.
PATRICi32117499. VBIEscCol129921_1172.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64519 Genomic DNA. Translation: AAC37038.1.
U00096 Genomic DNA. Translation: AAC74210.1.
AP009048 Genomic DNA. Translation: BAA35946.1.
PIRiA40840.
RefSeqiNP_415644.1. NC_000913.3.
WP_000531594.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP69874.
SMRiP69874. Positions 14-378.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261674. 3 interactions.
IntActiP69874. 2 interactions.
STRINGi511145.b1126.

Protein family/group databases

TCDBi3.A.1.11.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

EPDiP69874.
PaxDbiP69874.
PRIDEiP69874.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74210; AAC74210; b1126.
BAA35946; BAA35946; BAA35946.
GeneIDi946323.
KEGGiecj:JW1112.
eco:b1126.
PATRICi32117499. VBIEscCol129921_1172.

Organism-specific databases

EchoBASEiEB0742.
EcoGeneiEG10749. potA.

Phylogenomic databases

eggNOGiENOG4105C53. Bacteria.
COG3842. LUCA.
InParanoidiP69874.
KOiK11072.
OMAiIGYIRER.
OrthoDBiEOG6T7N3V.
PhylomeDBiP69874.

Enzyme and pathway databases

BioCyciEcoCyc:POTA-MONOMER.
ECOL316407:JW1112-MONOMER.
MetaCyc:POTA-MONOMER.

Miscellaneous databases

PROiP69874.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR017879. ABC_Sperm/Put_ATP-bd_PotA.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR008995. Mo/tungstate-bd_C_term_dom.
IPR027417. P-loop_NTPase.
IPR005893. Sp_pt_ABC_ATP-bd.
IPR013611. Transp-assoc_OB_typ2.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF08402. TOBE_2. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50331. SSF50331. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01187. potA. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51305. POTA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characteristics of the gene for a spermidine and putrescine transport system that maps at 15 min on the Escherichia coli chromosome."
    Furuchi T., Kashiwagi K., Kobayashi H., Igarashi K.
    J. Biol. Chem. 266:20928-20933(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN SPERMIDINE AND PUTRESCINE TRANSPORT, SUBCELLULAR LOCATION.
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Spermidine-preferential uptake system in Escherichia coli. ATP hydrolysis by PotA protein and its association with membranes."
    Kashiwagi K., Endo H., Kobayashi H., Takio K., Igarashi K.
    J. Biol. Chem. 270:25377-25382(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-30 AND 360-378, BIOPHYSICOCHEMICAL PROPERTIES, ATPASE ACTIVITY, ENZYME REGULATION, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-26; CYS-54 AND CYS-276.
    Strain: K12.
  6. "Isolation of polyamine transport-deficient mutants of Escherichia coli and cloning of the genes for polyamine transport proteins."
    Kashiwagi K., Hosokawa N., Furuchi T., Kobayashi H., Sasakawa C., Yoshikawa M., Igarashi K.
    J. Biol. Chem. 265:20893-20897(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN SPERMIDINE AND PUTRESCINE TRANSPORT, ATPASE ACTIVITY, ENZYME REGULATION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  7. "Functions of potA and potD proteins in spermidine-preferential uptake system in Escherichia coli."
    Kashiwagi K., Miyamoto S., Nukui E., Kobayashi H., Igarashi K.
    J. Biol. Chem. 268:19358-19363(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN SPERMIDINE TRANSPORT, ATPASE ACTIVITY, ENZYME REGULATION, MUTAGENESIS OF VAL-135.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  8. "Transcriptional inhibition of the operon for the spermidine uptake system by the substrate-binding protein PotD."
    Antognoni F., Del Duca S., Kuraishi A., Kawabe E., Fukuchi-Shimogori T., Kashiwagi K., Igarashi K.
    J. Biol. Chem. 274:1942-1948(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: TRANSCRIPTIONAL REGULATION BY POTD.
    Strain: K12.
  9. "The ATPase activity and the functional domain of PotA, a component of the spermidine-preferential uptake system in Escherichia coli."
    Kashiwagi K., Innami A., Zenda R., Tomitori H., Igarashi K.
    J. Biol. Chem. 277:24212-24219(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, ATPASE ACTIVITY, ENZYME REGULATION, MUTAGENESIS OF PHE-27; PHE-45; LEU-60; LEU-76; VAL-135; ASP-172 AND GLU-297.
    Strain: K12.

Entry informationi

Entry nameiPOTA_ECOLI
AccessioniPrimary (citable) accession number: P69874
Secondary accession number(s): P23858
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: December 21, 2004
Last modified: March 16, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.