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Protein

Probable N-acetylneuraminic acid outer membrane channel protein NanC

Gene

nanC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Outer membrane channel protein allowing the entry of N-acetylneuraminic acid (Neu5Ac, the most abundant sialic acid on host cell surfaces) into the bacteria (Probable). NanC proteins form high-conductance channels which are open at low membrane potentials and which have a weak anion selectivity.Curated1 Publication

GO - Molecular functioni

  • oligosaccharide transporting porin activity Source: EcoliWiki
  • porin activity Source: EcoliWiki
  • sialic acid transmembrane transporter activity Source: EcoliWiki

GO - Biological processi

  • ion transport Source: UniProtKB-KW
  • oligosaccharide transport Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Porin

Keywords - Biological processi

Ion transport, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G7921-MONOMER.
ECOL316407:JW5778-MONOMER.
MetaCyc:G7921-MONOMER.

Protein family/group databases

TCDBi1.B.35.2.1. the oligogalacturonate-specific porin (kdgm) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable N-acetylneuraminic acid outer membrane channel protein NanC
Alternative name(s):
NanR-regulated channel
Porin NanC
Gene namesi
Name:nanC
Synonyms:yjhA
Ordered Locus Names:b4311, JW5778
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12389. nanC.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24Periplasmic1 Publication1
Transmembranei25 – 339
Topological domaini34 – 37Extracellular1 Publication4
Transmembranei38 – 48Add BLAST11
Topological domaini49 – 52Periplasmic1 Publication4
Transmembranei53 – 64Add BLAST12
Topological domaini65 – 75Extracellular1 PublicationAdd BLAST11
Transmembranei76 – 87Add BLAST12
Topological domaini88 – 91Periplasmic1 Publication4
Transmembranei92 – 102Add BLAST11
Topological domaini103 – 105Extracellular1 Publication3
Transmembranei106 – 118Add BLAST13
Topological domaini119 – 122Periplasmic1 Publication4
Transmembranei123 – 134Add BLAST12
Topological domaini135 – 149Extracellular1 PublicationAdd BLAST15
Transmembranei150 – 160Add BLAST11
Topological domaini161 – 164Periplasmic1 Publication4
Transmembranei165 – 176Add BLAST12
Topological domaini177 – 187Extracellular1 PublicationAdd BLAST11
Transmembranei188 – 198Add BLAST11
Topological domaini199 – 203Periplasmic1 Publication5
Transmembranei204 – 2129
Topological domaini213 – 227Extracellular1 PublicationAdd BLAST15
Transmembranei228 – 2369
Topological domaini237 – 238Periplasmic1 Publication2

GO - Cellular componenti

  • integral component of cell outer membrane Source: EcoliWiki
  • pore complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000001660124 – 238Probable N-acetylneuraminic acid outer membrane channel protein NanCAdd BLAST215

Proteomic databases

PaxDbiP69856.
PRIDEiP69856.

Expressioni

Inductioni

Induced by N-acetylneuraminate and modulated by N-acetylglucosamine. This regulation occurs via the NanR and NagC regulators. NanC expression is also activated by the regulators cyclic AMP-catabolite activator protein, OmpR, and CpxR.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4259375. 217 interactors.
STRINGi511145.b4311.

Structurei

Secondary structure

1238
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi26 – 33Combined sources8
Turni34 – 37Combined sources4
Beta strandi38 – 48Combined sources11
Beta strandi52 – 64Combined sources13
Beta strandi77 – 84Combined sources8
Beta strandi86 – 103Combined sources18
Beta strandi106 – 120Combined sources15
Beta strandi123 – 136Combined sources14
Beta strandi150 – 160Combined sources11
Beta strandi162 – 179Combined sources18
Beta strandi185 – 198Combined sources14
Beta strandi200 – 212Combined sources13
Beta strandi217 – 219Combined sources3
Beta strandi222 – 225Combined sources4
Beta strandi228 – 236Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WJQX-ray2.00A24-238[»]
2WJRX-ray1.80A25-238[»]
ProteinModelPortaliP69856.
SMRiP69856.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69856.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG41069FU. Bacteria.
ENOG410XY8U. LUCA.
HOGENOMiHOG000127485.
OMAiGEDNISE.

Family and domain databases

InterProiIPR009331. Oligogalacturonate-sp_porin.
[Graphical view]
PfamiPF06178. KdgM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69856-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKAKILSGV LLLCFSSPLI SQAATLDVRG GYRSGSHAYE TRLKVSEGWQ
60 70 80 90 100
NGWWASMESN TWNTIHDNKK ENAALNDVQV EVNYAIKLDD QWTVRPGMLT
110 120 130 140 150
HFSSNGTRYG PYVKLSWDAT KDLNFGIRYR YDWKAYRQQD LSGDMSRDNV
160 170 180 190 200
HRWDGYVTYH INSDFTFAWQ TTLYSKQNDY RYANHKKWAT ENAFVLQYHM
210 220 230
TPDITPYIEY DYLDRQGVYN GRDNLSENSY RIGVSFKL
Length:238
Mass (Da):27,888
Last modified:June 7, 2005 - v1
Checksum:i52B992EF95AD62F7
GO

Sequence cautioni

The sequence AAA97207 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97207.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77267.2.
AP009048 Genomic DNA. Translation: BAE78304.1.
PIRiS56536.
RefSeqiNP_418731.2. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC77267; AAC77267; b4311.
BAE78304; BAE78304; BAE78304.
GeneIDi946843.
KEGGiecj:JW5778.
eco:b4311.
PATRICi32124216. VBIEscCol129921_4451.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97207.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77267.2.
AP009048 Genomic DNA. Translation: BAE78304.1.
PIRiS56536.
RefSeqiNP_418731.2. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WJQX-ray2.00A24-238[»]
2WJRX-ray1.80A25-238[»]
ProteinModelPortaliP69856.
SMRiP69856.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259375. 217 interactors.
STRINGi511145.b4311.

Protein family/group databases

TCDBi1.B.35.2.1. the oligogalacturonate-specific porin (kdgm) family.

Proteomic databases

PaxDbiP69856.
PRIDEiP69856.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77267; AAC77267; b4311.
BAE78304; BAE78304; BAE78304.
GeneIDi946843.
KEGGiecj:JW5778.
eco:b4311.
PATRICi32124216. VBIEscCol129921_4451.

Organism-specific databases

EchoBASEiEB2290.
EcoGeneiEG12389. nanC.

Phylogenomic databases

eggNOGiENOG41069FU. Bacteria.
ENOG410XY8U. LUCA.
HOGENOMiHOG000127485.
OMAiGEDNISE.

Enzyme and pathway databases

BioCyciEcoCyc:G7921-MONOMER.
ECOL316407:JW5778-MONOMER.
MetaCyc:G7921-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP69856.
PROiP69856.

Family and domain databases

InterProiIPR009331. Oligogalacturonate-sp_porin.
[Graphical view]
PfamiPF06178. KdgM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNANC_ECOLI
AccessioniPrimary (citable) accession number: P69856
Secondary accession number(s): P39372, Q2M602
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.