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Protein

Probable N-acetylneuraminic acid outer membrane channel protein NanC

Gene

nanC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Outer membrane channel protein allowing the entry of N-acetylneuraminic acid (Neu5Ac, the most abundant sialic acid on host cell surfaces) into the bacteria (Probable). NanC proteins form high-conductance channels which are open at low membrane potentials and which have a weak anion selectivity.Curated1 Publication

GO - Molecular functioni

  • oligosaccharide transporting porin activity Source: EcoliWiki
  • porin activity Source: EcoliWiki
  • sialic acid transmembrane transporter activity Source: EcoliWiki

GO - Biological processi

  • oligosaccharide transport Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Porin

Keywords - Biological processi

Ion transport, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G7921-MONOMER.
ECOL316407:JW5778-MONOMER.
MetaCyc:G7921-MONOMER.

Protein family/group databases

TCDBi1.B.35.2.1. the oligogalacturonate-specific porin (kdgm) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable N-acetylneuraminic acid outer membrane channel protein NanC
Alternative name(s):
NanR-regulated channel
Porin NanC
Gene namesi
Name:nanC
Synonyms:yjhA
Ordered Locus Names:b4311, JW5778
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12389. nanC.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 241Periplasmic1 Publication
Transmembranei25 – 339
Topological domaini34 – 374Extracellular1 Publication
Transmembranei38 – 4811Add
BLAST
Topological domaini49 – 524Periplasmic1 Publication
Transmembranei53 – 6412Add
BLAST
Topological domaini65 – 7511Extracellular1 PublicationAdd
BLAST
Transmembranei76 – 8712Add
BLAST
Topological domaini88 – 914Periplasmic1 Publication
Transmembranei92 – 10211Add
BLAST
Topological domaini103 – 1053Extracellular1 Publication
Transmembranei106 – 11813Add
BLAST
Topological domaini119 – 1224Periplasmic1 Publication
Transmembranei123 – 13412Add
BLAST
Topological domaini135 – 14915Extracellular1 PublicationAdd
BLAST
Transmembranei150 – 16011Add
BLAST
Topological domaini161 – 1644Periplasmic1 Publication
Transmembranei165 – 17612Add
BLAST
Topological domaini177 – 18711Extracellular1 PublicationAdd
BLAST
Transmembranei188 – 19811Add
BLAST
Topological domaini199 – 2035Periplasmic1 Publication
Transmembranei204 – 2129
Topological domaini213 – 22715Extracellular1 PublicationAdd
BLAST
Transmembranei228 – 2369
Topological domaini237 – 2382Periplasmic1 Publication

GO - Cellular componenti

  • integral component of cell outer membrane Source: EcoliWiki
  • pore complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 238215Probable N-acetylneuraminic acid outer membrane channel protein NanCPRO_0000016601Add
BLAST

Proteomic databases

PaxDbiP69856.

Expressioni

Inductioni

Induced by N-acetylneuraminate and modulated by N-acetylglucosamine. This regulation occurs via the NanR and NagC regulators. NanC expression is also activated by the regulators cyclic AMP-catabolite activator protein, OmpR, and CpxR.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4259375. 217 interactions.
STRINGi511145.b4311.

Structurei

Secondary structure

1
238
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi26 – 338Combined sources
Turni34 – 374Combined sources
Beta strandi38 – 4811Combined sources
Beta strandi52 – 6413Combined sources
Beta strandi77 – 848Combined sources
Beta strandi86 – 10318Combined sources
Beta strandi106 – 12015Combined sources
Beta strandi123 – 13614Combined sources
Beta strandi150 – 16011Combined sources
Beta strandi162 – 17918Combined sources
Beta strandi185 – 19814Combined sources
Beta strandi200 – 21213Combined sources
Beta strandi217 – 2193Combined sources
Beta strandi222 – 2254Combined sources
Beta strandi228 – 2369Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WJQX-ray2.00A24-238[»]
2WJRX-ray1.80A25-238[»]
ProteinModelPortaliP69856.
SMRiP69856. Positions 26-238.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69856.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG41069FU. Bacteria.
ENOG410XY8U. LUCA.
HOGENOMiHOG000127485.
OMAiGEDNISE.
OrthoDBiEOG6H1PZG.

Family and domain databases

InterProiIPR009331. Oligogalacturonate-sp_porin.
[Graphical view]
PfamiPF06178. KdgM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69856-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKAKILSGV LLLCFSSPLI SQAATLDVRG GYRSGSHAYE TRLKVSEGWQ
60 70 80 90 100
NGWWASMESN TWNTIHDNKK ENAALNDVQV EVNYAIKLDD QWTVRPGMLT
110 120 130 140 150
HFSSNGTRYG PYVKLSWDAT KDLNFGIRYR YDWKAYRQQD LSGDMSRDNV
160 170 180 190 200
HRWDGYVTYH INSDFTFAWQ TTLYSKQNDY RYANHKKWAT ENAFVLQYHM
210 220 230
TPDITPYIEY DYLDRQGVYN GRDNLSENSY RIGVSFKL
Length:238
Mass (Da):27,888
Last modified:June 7, 2005 - v1
Checksum:i52B992EF95AD62F7
GO

Sequence cautioni

The sequence AAA97207.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97207.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77267.2.
AP009048 Genomic DNA. Translation: BAE78304.1.
PIRiS56536.
RefSeqiNP_418731.2. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC77267; AAC77267; b4311.
BAE78304; BAE78304; BAE78304.
GeneIDi946843.
KEGGiecj:JW5778.
eco:b4311.
PATRICi32124216. VBIEscCol129921_4451.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97207.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77267.2.
AP009048 Genomic DNA. Translation: BAE78304.1.
PIRiS56536.
RefSeqiNP_418731.2. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WJQX-ray2.00A24-238[»]
2WJRX-ray1.80A25-238[»]
ProteinModelPortaliP69856.
SMRiP69856. Positions 26-238.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259375. 217 interactions.
STRINGi511145.b4311.

Protein family/group databases

TCDBi1.B.35.2.1. the oligogalacturonate-specific porin (kdgm) family.

Proteomic databases

PaxDbiP69856.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77267; AAC77267; b4311.
BAE78304; BAE78304; BAE78304.
GeneIDi946843.
KEGGiecj:JW5778.
eco:b4311.
PATRICi32124216. VBIEscCol129921_4451.

Organism-specific databases

EchoBASEiEB2290.
EcoGeneiEG12389. nanC.

Phylogenomic databases

eggNOGiENOG41069FU. Bacteria.
ENOG410XY8U. LUCA.
HOGENOMiHOG000127485.
OMAiGEDNISE.
OrthoDBiEOG6H1PZG.

Enzyme and pathway databases

BioCyciEcoCyc:G7921-MONOMER.
ECOL316407:JW5778-MONOMER.
MetaCyc:G7921-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP69856.
PROiP69856.

Family and domain databases

InterProiIPR009331. Oligogalacturonate-sp_porin.
[Graphical view]
PfamiPF06178. KdgM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Function and expression of an N-acetylneuraminic acid-inducible outer membrane channel in Escherichia coli."
    Condemine G., Berrier C., Plumbridge J., Ghazi A.
    J. Bacteriol. 187:1959-1965(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TRANSCRIPTIONAL REGULATION.
  5. "NanC crystal structure, a model for outer-membrane channels of the acidic sugar-specific KdgM porin family."
    Wirth C., Condemine G., Boiteux C., Berneche S., Schirmer T., Peneff C.M.
    J. Mol. Biol. 394:718-731(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 24-238, TOPOLOGY, SUBUNIT.

Entry informationi

Entry nameiNANC_ECOLI
AccessioniPrimary (citable) accession number: P69856
Secondary accession number(s): P39372, Q2M602
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: July 6, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.