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Protein

Tat proofreading chaperone DmsD

Gene

dmsD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for biogenesis/assembly of DMSO reductase, but not for the interaction of the DmsA signal peptide with the Tat system. May be part of a chaperone cascade complex that facilitates a folding-maturation pathway for the substrate protein.UniRule annotation4 Publications

GO - Molecular functioni

Keywordsi

Molecular functionChaperone

Enzyme and pathway databases

BioCyciEcoCyc:G6849-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Tat proofreading chaperone DmsDUniRule annotation
Alternative name(s):
DMSO reductase maturation proteinUniRule annotation
Twin-arginine leader-binding protein DmsDUniRule annotation
Gene namesi
Name:dmsDUniRule annotation
Synonyms:ynfI
Ordered Locus Names:b1591, JW5262
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13847 dmsD

Subcellular locationi

  • Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication
  • Cytoplasm 1 Publication
  • Note: Mainly cytoplasmic under aerobic conditions, and found in the inner membrane under anaerobic conditions.

GO - Cellular componenti

  • cytoplasm Source: EcoCyc
  • extrinsic component of cytoplasmic side of plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi72 – 75WQRL → HQRY: 1.5-fold increased binding to DmsA signal sequence. 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002116492 – 204Tat proofreading chaperone DmsDAdd BLAST203

Proteomic databases

PaxDbiP69853
PRIDEiP69853

Interactioni

Subunit structurei

Monomer and homodimer. Binds to the twin-arginine signal peptide of DmsA and TorA (PubMed:11309116), although the latter binding is controversial (PubMed:20169075). Interacts with the TatB and TatC subunits of the Tat translocase complex. Interacts also with other general chaperones, such as GroEL, and proteins involved in the molybdenum cofactor biosynthesis.5 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4259117, 3 interactors
DIPiDIP-47840N
IntActiP69853, 11 interactors
MINTiP69853
STRINGi316385.ECDH10B_1724

Structurei

Secondary structure

1204
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 6Combined sources3
Helixi10 – 22Combined sources13
Turni28 – 30Combined sources3
Helixi31 – 37Combined sources7
Helixi42 – 45Combined sources4
Beta strandi46 – 48Combined sources3
Helixi50 – 60Combined sources11
Helixi68 – 76Combined sources9
Helixi88 – 92Combined sources5
Helixi101 – 112Combined sources12
Helixi128 – 140Combined sources13
Helixi144 – 154Combined sources11
Helixi156 – 169Combined sources14
Helixi173 – 191Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CW0X-ray2.40A/B/C/D1-204[»]
3EFPX-ray2.01A/B1-204[»]
3U41X-ray2.50A/B/C/D/E/F/G/H1-204[»]
ProteinModelPortaliP69853
SMRiP69853
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69853

Family & Domainsi

Sequence similaritiesi

Belongs to the TorD/DmsD family. DmsD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108NEM Bacteria
COG3381 LUCA
HOGENOMiHOG000120828
InParanoidiP69853
OMAiAWHLLPW
PhylomeDBiP69853

Family and domain databases

HAMAPiMF_00940 DmsD_chaperone, 1 hit
InterProiView protein in InterPro
IPR026269 DmsD-type
IPR028611 DmsD_chaperone
IPR020945 DMSO/NO3_reduct_chaperone
IPR036411 TorD-like_sf
PfamiView protein in Pfam
PF02613 Nitrate_red_del, 1 hit
PIRSFiPIRSF004690 DmsD, 1 hit
SUPFAMiSSF89155 SSF89155, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69853-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHFSQQDNF SVAARVLGAL FYYAPESAEA APLVAVLTSD GWETQWPLPE
60 70 80 90 100
ASLAPLVTAF QTQCEETHAQ AWQRLFVGPW ALPSPPWGSV WLDRESVLFG
110 120 130 140 150
DSTLALRQWM REKGIQFEMK QNEPEDHFGS LLLMAAWLAE NGRQTECEEL
160 170 180 190 200
LAWHLFPWST RFLDVFIEKA EHPFYRALGE LARLTLAQWQ SQLLIPVAVK

PLFR
Length:204
Mass (Da):23,345
Last modified:January 23, 2007 - v2
Checksum:iCB4273F4B6539D47
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7Missing AA sequence (PubMed:11309116).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74663.2
AP009048 Genomic DNA Translation: BAA15315.1
PIRiA64915
RefSeqiNP_416108.2, NC_000913.3
WP_000148710.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74663; AAC74663; b1591
BAA15315; BAA15315; BAA15315
GeneIDi945987
KEGGiecj:JW5262
eco:b1591
PATRICifig|1411691.4.peg.671

Similar proteinsi

Entry informationi

Entry nameiDMSD_ECOLI
AccessioniPrimary (citable) accession number: P69853
Secondary accession number(s): P76174, P77270
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 99 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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