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Protein

Tat proofreading chaperone DmsD

Gene

dmsD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for biogenesis/assembly of DMSO reductase, but not for the interaction of the DmsA signal peptide with the Tat system. May be part of a chaperone cascade complex that facilitates a folding-maturation pathway for the substrate protein.UniRule annotation4 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

BioCyciEcoCyc:G6849-MONOMER.
ECOL316407:JW5262-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tat proofreading chaperone DmsDUniRule annotation
Alternative name(s):
DMSO reductase maturation proteinUniRule annotation
Twin-arginine leader-binding protein DmsDUniRule annotation
Gene namesi
Name:dmsDUniRule annotation
Synonyms:ynfI
Ordered Locus Names:b1591, JW5262
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13847. dmsD.

Subcellular locationi

  • Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication
  • Cytoplasm 1 Publication

  • Note: Mainly cytoplasmic under aerobic conditions, and found in the inner membrane under anaerobic conditions.

GO - Cellular componenti

  • cytoplasm Source: EcoCyc
  • extrinsic component of cytoplasmic side of plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi72 – 75WQRL → HQRY: 1.5-fold increased binding to DmsA signal sequence. 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002116492 – 204Tat proofreading chaperone DmsDAdd BLAST203

Proteomic databases

PaxDbiP69853.
PRIDEiP69853.

Interactioni

Subunit structurei

Monomer and homodimer. Binds to the twin-arginine signal peptide of DmsA and TorA (PubMed:11309116), although the latter binding is controversial (PubMed:20169075). Interacts with the TatB and TatC subunits of the Tat translocase complex. Interacts also with other general chaperones, such as GroEL, and proteins involved in the molybdenum cofactor biosynthesis.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
dmsAP187757EBI-4406374,EBI-4411104
groLC5A1D55EBI-4406374,EBI-4406290From a different organism.
grpEC4ZYN14EBI-4406374,EBI-4407105From a different organism.
tigC4ZTJ33EBI-4406374,EBI-4407188From a different organism.
ynfEP773743EBI-4406374,EBI-556186
ynfFP777832EBI-4406374,EBI-6406285

Protein-protein interaction databases

BioGridi4259117. 3 interactors.
DIPiDIP-47840N.
IntActiP69853. 10 interactors.
MINTiMINT-8046358.
STRINGi511145.b1591.

Structurei

Secondary structure

1204
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 6Combined sources3
Helixi10 – 22Combined sources13
Turni28 – 30Combined sources3
Helixi31 – 37Combined sources7
Helixi42 – 45Combined sources4
Beta strandi46 – 48Combined sources3
Helixi50 – 60Combined sources11
Helixi68 – 76Combined sources9
Helixi88 – 92Combined sources5
Helixi101 – 112Combined sources12
Helixi128 – 140Combined sources13
Helixi144 – 154Combined sources11
Helixi156 – 169Combined sources14
Helixi173 – 191Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CW0X-ray2.40A/B/C/D1-204[»]
3EFPX-ray2.01A/B1-204[»]
3U41X-ray2.50A/B/C/D/E/F/G/H1-204[»]
ProteinModelPortaliP69853.
SMRiP69853.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69853.

Family & Domainsi

Sequence similaritiesi

Belongs to the TorD/DmsD family. DmsD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108NEM. Bacteria.
COG3381. LUCA.
HOGENOMiHOG000120828.
InParanoidiP69853.
OMAiAWHLLPW.
PhylomeDBiP69853.

Family and domain databases

Gene3Di1.10.3480.10. 1 hit.
HAMAPiMF_00940. DmsD_chaperone. 1 hit.
InterProiIPR026269. DmsD-type.
IPR028611. DmsD_chaperone.
IPR020945. DMSO/NO3_reduct_chaperone.
[Graphical view]
PfamiPF02613. Nitrate_red_del. 1 hit.
[Graphical view]
PIRSFiPIRSF004690. DmsD. 1 hit.
SUPFAMiSSF89155. SSF89155. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69853-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHFSQQDNF SVAARVLGAL FYYAPESAEA APLVAVLTSD GWETQWPLPE
60 70 80 90 100
ASLAPLVTAF QTQCEETHAQ AWQRLFVGPW ALPSPPWGSV WLDRESVLFG
110 120 130 140 150
DSTLALRQWM REKGIQFEMK QNEPEDHFGS LLLMAAWLAE NGRQTECEEL
160 170 180 190 200
LAWHLFPWST RFLDVFIEKA EHPFYRALGE LARLTLAQWQ SQLLIPVAVK

PLFR
Length:204
Mass (Da):23,345
Last modified:January 23, 2007 - v2
Checksum:iCB4273F4B6539D47
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7Missing AA sequence (PubMed:11309116).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74663.2.
AP009048 Genomic DNA. Translation: BAA15315.1.
PIRiA64915.
RefSeqiNP_416108.2. NC_000913.3.
WP_000148710.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74663; AAC74663; b1591.
BAA15315; BAA15315; BAA15315.
GeneIDi945987.
KEGGiecj:JW5262.
eco:b1591.
PATRICi32118484. VBIEscCol129921_1662.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74663.2.
AP009048 Genomic DNA. Translation: BAA15315.1.
PIRiA64915.
RefSeqiNP_416108.2. NC_000913.3.
WP_000148710.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CW0X-ray2.40A/B/C/D1-204[»]
3EFPX-ray2.01A/B1-204[»]
3U41X-ray2.50A/B/C/D/E/F/G/H1-204[»]
ProteinModelPortaliP69853.
SMRiP69853.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259117. 3 interactors.
DIPiDIP-47840N.
IntActiP69853. 10 interactors.
MINTiMINT-8046358.
STRINGi511145.b1591.

Proteomic databases

PaxDbiP69853.
PRIDEiP69853.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74663; AAC74663; b1591.
BAA15315; BAA15315; BAA15315.
GeneIDi945987.
KEGGiecj:JW5262.
eco:b1591.
PATRICi32118484. VBIEscCol129921_1662.

Organism-specific databases

EchoBASEiEB3608.
EcoGeneiEG13847. dmsD.

Phylogenomic databases

eggNOGiENOG4108NEM. Bacteria.
COG3381. LUCA.
HOGENOMiHOG000120828.
InParanoidiP69853.
OMAiAWHLLPW.
PhylomeDBiP69853.

Enzyme and pathway databases

BioCyciEcoCyc:G6849-MONOMER.
ECOL316407:JW5262-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP69853.
PROiP69853.

Family and domain databases

Gene3Di1.10.3480.10. 1 hit.
HAMAPiMF_00940. DmsD_chaperone. 1 hit.
InterProiIPR026269. DmsD-type.
IPR028611. DmsD_chaperone.
IPR020945. DMSO/NO3_reduct_chaperone.
[Graphical view]
PfamiPF02613. Nitrate_red_del. 1 hit.
[Graphical view]
PIRSFiPIRSF004690. DmsD. 1 hit.
SUPFAMiSSF89155. SSF89155. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDMSD_ECOLI
AccessioniPrimary (citable) accession number: P69853
Secondary accession number(s): P76174, P77270
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.