2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic precursor

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P69834 (ISPD_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 62. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=AtMECT
Short name=AtMEPCT
Short name=MCT

Gene names

Name:	ISPD
Synonyms:	MECT, MEPCT
Ordered Locus Names:	At2g02500
ORF Names:	T8K22.20

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP). Involved in pigments and gibberellins biosynthesis. Ref.1 Ref.2
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.
Cofactor	Divalent metal cations. Magnesium, nickel and manganese are the most effective. Cobalt and calcium are only minimally effective. Ref.1
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6.
Subcellular location	Plastid › chloroplast Probable.
Tissue specificity	Expressed in leaves, stems and flowers, but not in roots. Ref.2
Sequence similarities	Belongs to the IspD family.
Biophysicochemical properties	Kinetic parameters: K_M=114 µM for CTP Ref.1 K_M=500 µM for MEP V_max=67 µmol/min/mg enzyme

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Nucleotidyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Traceable author statement Ref.1. Source: TAIR
Cellular component	chloroplast stroma Inferred from direct assay. Source: TAIR
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from direct assay Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 61

Chloroplast Potential

Chain

62 – 302

241

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic

PRO_0000016478

Sites

Site

Transition state stabilizer By similarity

Site

Transition state stabilizer By similarity

Site

228

Positions MEP for the nucleophilic attack By similarity

Site

284

Positions MEP for the nucleophilic attack By similarity

Secondary structure

302

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P69834 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 7881DC5C8CE37B06
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FASTA

302

33,937

        10         20         30         40         50         60 
MAMLQTNLGF ITSPTFLCPK LKVKLNSYLW FSYRSQVQKL DFSKRVNRSY KRDALLLSIK 

        70         80         90        100        110        120 
CSSSTGFDNS NVVVKEKSVS VILLAGGQGK RMKMSMPKQY IPLLGQPIAL YSFFTFSRMP 

       130        140        150        160        170        180 
EVKEIVVVCD PFFRDIFEEY EESIDVDLRF AIPGKERQDS VYSGLQEIDV NSELVCIHDS 

       190        200        210        220        230        240 
ARPLVNTEDV EKVLKDGSAV GAAVLGVPAK ATIKEVNSDS LVVKTLDRKT LWEMQTPQVI 

       250        260        270        280        290        300 
KPELLKKGFE LVKSEGLEVT DDVSIVEYLK HPVYVSQGSY TNIKVTTPDD LLLAERILSE 


DS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biosynthesis of terpenoids: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase of Arabidopsis thaliana." Rohdich F., Wungsintaweekul J., Eisenreich W., Richter G., Schuhr C.A., Hecht S., Zenk M.H., Bacher A. Proc. Natl. Acad. Sci. U.S.A. 97:6451-6456(2000) [PubMed: 10841550] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES. Strain: cv. Columbia.
[2]	"Antisense and chemical suppression of the nonmevalonate pathway affects ent-kaurene biosynthesis in Arabidopsis." Okada K., Kawaide H., Kuzuyama T., Seto H., Curtis I.S., Kamiya Y. Planta 215:339-344(2002) [PubMed: 12029484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF230737 mRNA. Translation: AAF61714.1.
AB037876 mRNA. Translation: BAB21592.1.
AC004136 Genomic DNA. Translation: AAC18936.2.
CP002685 Genomic DNA. Translation: AEC05588.1.
AK118110 mRNA. Translation: BAC42737.1.
BT006120 mRNA. Translation: AAP04105.1.

IPI

IPI00546361.

PIR

T00613.

RefSeq

NP_565286.1. NM_126305.2.

UniGene

At.10212.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1W77	X-ray	2.00	A	76-302	[»]
2YC3	X-ray	1.40	A	76-302	[»]
2YC5	X-ray	1.60	A	76-302	[»]
2YCM	X-ray	1.80	A	76-302	[»]

ProteinModelPortal

P69834.

SMR

P69834. Positions 76-300.

ModBase

Search...

Protein-protein interaction databases

STRING

P69834.

Proteomic databases

PRIDE

P69834.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT2G02500.1; AT2G02500.1; AT2G02500.

GeneID

814779.

GenomeReviews

Gene locus AT2G02500 in contig CT485783_GR.

KEGG

ath:AT2G02500.

NMPDR

fig|3702.1.peg.7845.

Organism-specific databases

GeneFarm

4908.

TAIR

At2g02500.

Phylogenomic databases

eggNOG

euNOG05012.

GeneTree

EPGT00050000014038.

HOGENOM

HBG672839.

InParanoid

P69834.

OMA

SKVIVVC.

PhylomeDB

P69834.

ProtClustDB

PLN02728.

Enzyme and pathway databases

BioCyc

ARA:AT2G02500-MONOMER.
MetaCyc:AT2G02500-MONOMER.

Gene expression databases

Genevestigator

P69834.

GermOnline

AT2G02500. Arabidopsis thaliana.

Family and domain databases

InterPro

IPR001228. ISPD_synthase.
IPR018294. ISPD_synthase_CS.
[Graphical view]

K00991.

Pfam

PF01128. IspD. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00453. IspD. 1 hit.

PROSITE

PS01295. ISPD. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ISPD_ARATH

Accession

Primary (citable) accession number: P69834
Secondary accession number(s): O64726, Q9LL91

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2005
Last sequence update:	May 24, 2005
Last modified:	November 16, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents