Nitrogen regulatory protein - Escherichia coli (strain K12)

Contribute

Send feedback

Read comments (?) or add your own

P69829 (PTSN_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 65. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Nitrogen regulatory protein
EC=2.7.1.-

Alternative name(s):
Enzyme IIA-NTR
PTS system EIIA component
Phosphotransferase enzyme IIA component

Gene names

Name:	ptsN
Synonyms:	rpoP, yhbI
Ordered Locus Names:	b3204, JW3171

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	163 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Seems to have a role in regulating nitrogen assimilation By similarity.
Subcellular location	Cytoplasm.
Domain	The EIIA domain is phosphorylated by phospho-NPr on a histidyl residue.
Sequence similarities	Contains 1 PTS EIIA type-2 domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: InterPro sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 163

163

Nitrogen regulatory protein

PRO_0000186694

Regions

Domain

12 – 156

145

PTS EIIA type-2

Sites

Active site

Tele-phosphohistidine intermediate By similarity

Secondary structure

163

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P69829 [UniParc].

Last modified May 10, 2005. Version 1.
Checksum: 57240CF2C550AC80
Show »

FASTA

163

17,960

        10         20         30         40         50         60 
MTNNDTTLQL SSVLNRECTR SRVHCQSKKR ALEIISELAA KQLSLPPQVV FEAILTREKM 

        70         80         90        100        110        120 
GSTGIGNGIA IPHGKLEEDT LRAVGVFVQL ETPIAFDAID NQPVDLLFAL LVPADQTKTH 

       130        140        150        160 
LHTLSLVAKR LADKTICRRL RAAQSDEELY QIITDTEGTP DEA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli. Enzyme IIANtr affects growth on organic nitrogen and the conditional lethality of an erats mutant." Powell B.S., Court D.L., Inada T., Nakamura Y., Michotey V., Cui X., Reizer A., Saier M.H. Jr., Reizer J. J. Biol. Chem. 270:4822-4839(1995) [PubMed: 7876255] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Physical map location of the rpoN gene of Escherichia coli." Imaishi H., Gomada M., Inouye S., Nakazawa A. J. Bacteriol. 175:1550-1551(1993) [PubMed: 8444818] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"Molecular analysis of the operon which encodes the RNA polymerase sigma factor sigma 54 of Escherichia coli." Jones D.H.A., Franklin C.F.H., Thomas C.M. Microbiology 140:1035-1043(1994) [PubMed: 8025669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"The three-dimensional structure of the nitrogen regulatory protein IIANtr from Escherichia coli." Bordo D., van Monfort R.L., Pijning T., Kalk K.H., Reizer J., Saier M.H. Jr., Dijkstra B.W. J. Mol. Biol. 279:245-255(1998) [PubMed: 9636714] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS). Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U12684 Genomic DNA. Translation: AAB60165.1.
D12938 Genomic DNA. Translation: BAA02317.1.
Z27094 Genomic DNA. Translation: CAA81619.1.
U18997 Genomic DNA. Translation: AAA58006.1.
U00096 Genomic DNA. Translation: AAC76236.1.
AP009048 Genomic DNA. Translation: BAE77248.1.

PIR

I76720.

RefSeq

NP_417671.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A6J	X-ray	2.35	A/B	1-163	[»]

ProteinModelPortal

P69829.

SMR

P69829. Positions 1-157.

ModBase

Search...

Protein-protein interaction databases

IntAct

P69829. 2 interactions.

MINT

MINT-1239820.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000002771; EBESCP00000002771; EBESCG00000002261.
EBESCT00000002772; EBESCP00000002772; EBESCG00000002261.
EBESCT00000018209; EBESCP00000017500; EBESCG00000017264.

GeneID

947721.

GenomeReviews

Gene locus JW3171 in contig AP009048_GR.
Gene locus b3204 in contig U00096_GR.

KEGG

ecj:JW3171.
eco:b3204.

PATRIC

32121828. VBIEscCol129921_3298.

Organism-specific databases

EchoBASE

EB1633.

EcoGene

EG11682. ptsN.

Phylogenomic databases

eggNOG

COG1762.

GeneTree

EBGT00050000009031.

HOGENOM

HBG697201.

OMA

CTSKKRA.

PhylomeDB

P69829.

ProtClustDB

PRK10896.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11682-MONOMER.

Gene expression databases

Genevestigator

P69829.

Family and domain databases

InterPro

IPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_2.
IPR006320. PTS_Nitro_regul.
[Graphical view]

Gene3D

G3DSA:3.40.930.10. PTS_EIIA_2. 1 hit.

K02806.

Pfam

PF00359. PTS_EIIA_2. 1 hit.
[Graphical view]

SUPFAM

SSF55804. PTrfase/Anion_transptr. 1 hit.

TIGRFAMs

TIGR01419. Nitro_reg_IIA. 1 hit.

PROSITE

PS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PTSN_ECOLI

Accession

Primary (citable) accession number: P69829
Secondary accession number(s): P31222, Q2M908

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 10, 2005
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents