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Protein

Nitrogen regulatory protein

Gene

ptsN

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Seems to have a role in regulating nitrogen assimilation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei73Tele-phosphohistidine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • enzyme inhibitor activity Source: EcoCyc
  • kinase activity Source: UniProtKB-KW
  • protein kinase activator activity Source: EcoCyc
  • protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Source: InterPro

GO - Biological processi

  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: InterPro
  • regulation of ion transmembrane transporter activity Source: EcoCyc
  • response to organonitrogen compound Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:EG11682-MONOMER.
ECOL316407:JW3171-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen regulatory protein (EC:2.7.1.-)
Alternative name(s):
Enzyme IIA-NTR
PTS system EIIA component
Phosphotransferase enzyme IIA component
Gene namesi
Name:ptsN
Synonyms:rpoP, yhbI
Ordered Locus Names:b3204, JW3171
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11682. ptsN.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001866941 – 163Nitrogen regulatory proteinAdd BLAST163

Proteomic databases

EPDiP69829.
PaxDbiP69829.
PRIDEiP69829.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
kdpDP218654EBI-547017,EBI-1123100

Protein-protein interaction databases

BioGridi4259284. 17 interactors.
DIPiDIP-10604N.
IntActiP69829. 9 interactors.
MINTiMINT-1239820.
STRINGi511145.b3204.

Structurei

Secondary structure

1163
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 12Combined sources3
Helixi16 – 18Combined sources3
Beta strandi19 – 23Combined sources5
Helixi28 – 43Combined sources16
Helixi47 – 58Combined sources12
Beta strandi64 – 66Combined sources3
Beta strandi73 – 76Combined sources4
Helixi78 – 80Combined sources3
Beta strandi84 – 94Combined sources11
Beta strandi104 – 113Combined sources10
Helixi114 – 116Combined sources3
Turni117 – 120Combined sources4
Helixi121 – 131Combined sources11
Helixi134 – 142Combined sources9
Helixi146 – 154Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A6JX-ray2.35A/B1-163[»]
ProteinModelPortaliP69829.
SMRiP69829.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69829.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 156PTS EIIA type-2PROSITE-ProRule annotationAdd BLAST145

Domaini

The EIIA domain is phosphorylated by phospho-NPr on a histidyl residue.

Sequence similaritiesi

Contains 1 PTS EIIA type-2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4107RM2. Bacteria.
COG1762. LUCA.
HOGENOMiHOG000227559.
InParanoidiP69829.
KOiK02806.
OMAiPHCRLAG.
PhylomeDBiP69829.

Family and domain databases

Gene3Di3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
IPR006320. PTS_Nitro_regul.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
[Graphical view]
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR01419. nitro_reg_IIA. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69829-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNNDTTLQL SSVLNRECTR SRVHCQSKKR ALEIISELAA KQLSLPPQVV
60 70 80 90 100
FEAILTREKM GSTGIGNGIA IPHGKLEEDT LRAVGVFVQL ETPIAFDAID
110 120 130 140 150
NQPVDLLFAL LVPADQTKTH LHTLSLVAKR LADKTICRRL RAAQSDEELY
160
QIITDTEGTP DEA
Length:163
Mass (Da):17,960
Last modified:May 10, 2005 - v1
Checksum:i57240CF2C550AC80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12684 Genomic DNA. Translation: AAB60165.1.
D12938 Genomic DNA. Translation: BAA02317.1.
Z27094 Genomic DNA. Translation: CAA81619.1.
U18997 Genomic DNA. Translation: AAA58006.1.
U00096 Genomic DNA. Translation: AAC76236.1.
AP009048 Genomic DNA. Translation: BAE77248.1.
PIRiI76720.
RefSeqiNP_417671.1. NC_000913.3.
WP_000183676.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76236; AAC76236; b3204.
BAE77248; BAE77248; BAE77248.
GeneIDi947721.
KEGGiecj:JW3171.
eco:b3204.
PATRICi32121828. VBIEscCol129921_3298.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12684 Genomic DNA. Translation: AAB60165.1.
D12938 Genomic DNA. Translation: BAA02317.1.
Z27094 Genomic DNA. Translation: CAA81619.1.
U18997 Genomic DNA. Translation: AAA58006.1.
U00096 Genomic DNA. Translation: AAC76236.1.
AP009048 Genomic DNA. Translation: BAE77248.1.
PIRiI76720.
RefSeqiNP_417671.1. NC_000913.3.
WP_000183676.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A6JX-ray2.35A/B1-163[»]
ProteinModelPortaliP69829.
SMRiP69829.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259284. 17 interactors.
DIPiDIP-10604N.
IntActiP69829. 9 interactors.
MINTiMINT-1239820.
STRINGi511145.b3204.

Proteomic databases

EPDiP69829.
PaxDbiP69829.
PRIDEiP69829.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76236; AAC76236; b3204.
BAE77248; BAE77248; BAE77248.
GeneIDi947721.
KEGGiecj:JW3171.
eco:b3204.
PATRICi32121828. VBIEscCol129921_3298.

Organism-specific databases

EchoBASEiEB1633.
EcoGeneiEG11682. ptsN.

Phylogenomic databases

eggNOGiENOG4107RM2. Bacteria.
COG1762. LUCA.
HOGENOMiHOG000227559.
InParanoidiP69829.
KOiK02806.
OMAiPHCRLAG.
PhylomeDBiP69829.

Enzyme and pathway databases

BioCyciEcoCyc:EG11682-MONOMER.
ECOL316407:JW3171-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP69829.
PROiP69829.

Family and domain databases

Gene3Di3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
IPR006320. PTS_Nitro_regul.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
[Graphical view]
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR01419. nitro_reg_IIA. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTSN_ECOLI
AccessioniPrimary (citable) accession number: P69829
Secondary accession number(s): P31222, Q2M908
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.