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Protein

PTS system galactitol-specific EIIA component

Gene

gatA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. The enzyme II complex composed of GatA, GatB and GatC is involved in galactitol transport. It can also use D-glucitol.2 Publications

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + galactitol(Side 1) = [protein]-L-histidine + galactitol 1-phosphate(Side 2).1 Publication

Kineticsi

  1. KM=4.4 µM for D-galactitol1 Publication
  2. KM=800 µM for D-glucitol1 Publication
  1. Vmax=3.2 nmol/min/mg enzyme with D-galactitol as substrate1 Publication
  2. Vmax=3.2 nmol/min/mg enzyme with D-glucitol as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei62Tele-phosphohistidine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • galactitol metabolic process Source: UniProtKB-KW
  • galactitol transport Source: EcoCyc
  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Galactitol metabolism, Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:GATA-MONOMER.
ECOL316407:JW2078-MONOMER.
ECOL316407:JW2081-MONOMER.
MetaCyc:GATA-MONOMER.

Protein family/group databases

TCDBi4.A.5.1.1. the pts galactitol (gat) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system galactitol-specific EIIA component1 Publication
Alternative name(s):
EIIB-Gat1 Publication
Galactitol-specific phosphotransferase enzyme IIA component1 Publication (EC:2.7.1.2001 Publication)
Gene namesi
Name:gatA1 Publication
Ordered Locus Names:b2094, JW2078/JW2081
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12414. gatA.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001865761 – 150PTS system galactitol-specific EIIA componentAdd BLAST150

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei62Phosphohistidine; by HPrCurated1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP69828.
PRIDEiP69828.

Expressioni

Inductioni

Constitutively expressed.1 Publication

Interactioni

Subunit structurei

Forms a complex with one each of subunit of GatA, GatB and 2 subunits of GatC.1 Publication

Protein-protein interaction databases

DIPiDIP-47900N.
IntActiP69828. 3 interactors.
MINTiMINT-1300341.
STRINGi511145.b2094.

Structurei

3D structure databases

ProteinModelPortaliP69828.
SMRiP69828.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 144PTS EIIA type-2PROSITE-ProRule annotationAdd BLAST144

Domaini

The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 PTS EIIA type-2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG41082GB. Bacteria.
COG1762. LUCA.
HOGENOMiHOG000122110.
KOiK02773.
OMAiENMAVAI.
PhylomeDBiP69828.

Family and domain databases

Gene3Di3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
[Graphical view]
SUPFAMiSSF55804. SSF55804. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69828-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNLFVRSGI SFVDRSEVLT HIGNEMLAKG VVHDTWPQAL IAREAEFPTG
60 70 80 90 100
IMLEQHAIAI PHCEAIHAKS SAIYLLRPTN KVHFQQADDD NDVAVSLVIA
110 120 130 140 150
LIVENPQQQL KLLRCLFGKL QQPDIVETLI TLPETQLKEY FTKYVLDSDE
Length:150
Mass (Da):16,907
Last modified:May 10, 2005 - v1
Checksum:i1A760676BE5FB61D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti124D → E in CAA56228 (PubMed:7772602).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79837 Genomic DNA. Translation: CAA56228.1.
U00096 Genomic DNA. Translation: AAC75155.1.
AP009048 Genomic DNA. Translation: BAA15964.1. Sequence problems.
AP009048 Genomic DNA. Translation: BAA15957.2. Sequence problems.
PIRiE64976.
S55903.
RefSeqiNP_416597.1. NC_000913.3.
WP_000182899.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75155; AAC75155; b2094.
BAA15957; BAA15957; BAA15957.
BAA15964; BAA15964; BAA15964.
GeneIDi946633.
KEGGiecj:JW2078.
ecj:JW2081.
eco:b2094.
PATRICi32119521. VBIEscCol129921_2171.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79837 Genomic DNA. Translation: CAA56228.1.
U00096 Genomic DNA. Translation: AAC75155.1.
AP009048 Genomic DNA. Translation: BAA15964.1. Sequence problems.
AP009048 Genomic DNA. Translation: BAA15957.2. Sequence problems.
PIRiE64976.
S55903.
RefSeqiNP_416597.1. NC_000913.3.
WP_000182899.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP69828.
SMRiP69828.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-47900N.
IntActiP69828. 3 interactors.
MINTiMINT-1300341.
STRINGi511145.b2094.

Protein family/group databases

TCDBi4.A.5.1.1. the pts galactitol (gat) family.

Proteomic databases

PaxDbiP69828.
PRIDEiP69828.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75155; AAC75155; b2094.
BAA15957; BAA15957; BAA15957.
BAA15964; BAA15964; BAA15964.
GeneIDi946633.
KEGGiecj:JW2078.
ecj:JW2081.
eco:b2094.
PATRICi32119521. VBIEscCol129921_2171.

Organism-specific databases

EchoBASEiEB2313.
EcoGeneiEG12414. gatA.

Phylogenomic databases

eggNOGiENOG41082GB. Bacteria.
COG1762. LUCA.
HOGENOMiHOG000122110.
KOiK02773.
OMAiENMAVAI.
PhylomeDBiP69828.

Enzyme and pathway databases

BioCyciEcoCyc:GATA-MONOMER.
ECOL316407:JW2078-MONOMER.
ECOL316407:JW2081-MONOMER.
MetaCyc:GATA-MONOMER.

Miscellaneous databases

PROiP69828.

Family and domain databases

Gene3Di3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
[Graphical view]
SUPFAMiSSF55804. SSF55804. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTKA_ECOLI
AccessioniPrimary (citable) accession number: P69828
Secondary accession number(s): P37187
, P76413, P94757, P97243
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

In strain W3110, the sequence is interrupted by the insertion of an IS5 element between positions 69 and 70.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.