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Protein

PTS system mannitol-specific cryptic EIICB component

Gene

cmtA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport.By similarity

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-mannitol(Side 1) = [protein]-L-histidine + D-mannitol 1-phosphate(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei377 – 3771Phosphocysteine intermediate; for EIIB activityBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:CMTA-MONOMER.
ECOL316407:JW2900-MONOMER.
MetaCyc:CMTA-MONOMER.

Protein family/group databases

TCDBi4.A.2.1.24. the pts fructose-mannitol (fru) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system mannitol-specific cryptic EIICB component
Alternative name(s):
EIICB-Mtl
Short name:
EII-Mtl
Including the following 2 domains:
Mannitol permease IIC component
Alternative name(s):
PTS system mannitol-specific EIIC component
Mannitol-specific phosphotransferase enzyme IIB component (EC:2.7.1.197)
Alternative name(s):
PTS system mannitol-specific EIIB component
Gene namesi
Name:cmtA
Ordered Locus Names:b2933, JW2900
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11792. cmtA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2525CytoplasmicSequence analysisAdd
BLAST
Transmembranei26 – 4520HelicalCuratedAdd
BLAST
Topological domaini46 – 516PeriplasmicSequence analysis
Transmembranei52 – 7019HelicalCuratedAdd
BLAST
Topological domaini71 – 13565CytoplasmicSequence analysisAdd
BLAST
Transmembranei136 – 15520HelicalCuratedAdd
BLAST
Topological domaini156 – 16611PeriplasmicSequence analysisAdd
BLAST
Transmembranei167 – 18519HelicalCuratedAdd
BLAST
Topological domaini186 – 27388CytoplasmicSequence analysisAdd
BLAST
Transmembranei274 – 29421HelicalCuratedAdd
BLAST
Topological domaini295 – 31622PeriplasmicSequence analysisAdd
BLAST
Transmembranei317 – 33620HelicalCuratedAdd
BLAST
Topological domaini337 – 462126CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462PTS system mannitol-specific cryptic EIICB componentPRO_0000186679Add
BLAST

Proteomic databases

PaxDbiP69826.

Interactioni

Protein-protein interaction databases

BioGridi4262068. 76 interactions.
IntActiP69826. 3 interactions.
STRINGi511145.b2933.

Structurei

3D structure databases

ProteinModelPortaliP69826.
SMRiP69826. Positions 368-462.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 344332PTS EIIC type-2PROSITE-ProRule annotationAdd
BLAST
Domaini371 – 46191PTS EIIB type-2PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

Sequence similaritiesi

Contains 1 PTS EIIB type-2 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIC type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CTS. Bacteria.
COG2213. LUCA.
HOGENOMiHOG000252814.
KOiK02799.
K02800.
OMAiDIVVTHE.
OrthoDBiEOG6FBWWQ.
PhylomeDBiP69826.

Family and domain databases

Gene3Di3.40.50.10370. 1 hit.
InterProiIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR029503. PTS_EIIB_mannitol.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR004718. PTS_IIC_mtl.
[Graphical view]
PfamiPF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
TIGRFAMsiTIGR00851. mtlA. 1 hit.
PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69826-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENKSARAKV QAFGGFLTAM VIPNIGAFIA WGFITALFIP TGWLPNEHFA
60 70 80 90 100
KIVGPMITYL LPVMIGSTGG HLVGGKRGAV MGGIGTIGVI VGAEIPMFLG
110 120 130 140 150
SMIMGPLGGL VIKYVDKALE KRIPAGFEMV INNFSLGIAG MLLCLLGFEV
160 170 180 190 200
IGPAVLIANT FVKECIEALV HAGYLPLLSV INEPAKVLFL NNAIDQGVYY
210 220 230 240 250
PLGMQQASVN GKSIFFMVAS NPGPGLGLLL AFTLFGKGMS KRSAPGAMII
260 270 280 290 300
HFLGGIHELY FPYVLMKPLT IIAMIAGGMS GTWMFNLLDG GLVAGPSPGS
310 320 330 340 350
IFAYLALTPK GSFLATIAGV TVGTLVSFAI TSLILKMEKT VETESEDEFA
360 370 380 390 400
QSANAVKAMK QEGAFSLSRV KRIAFVCDAG MGSSAMGATT FRKRLEKAGL
410 420 430 440 450
AIEVKHYAIE NVPADADIVV THASLEGRVK RVTDKPLILI NNYIGDPKLD
460
TLFNQLTAEH KH
Length:462
Mass (Da):48,971
Last modified:May 10, 2005 - v1
Checksum:i83F2D203D9E5A4C4
GO

Sequence cautioni

The sequence CAA51229.1 differs from that shown.Contaminating sequence. Vector contamination at the C-terminus.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti274 – 2752MI → IEF in CAA51229 (PubMed:8353127).Curated
Sequence conflicti415 – 43521DADIV…RVTDK → GCRISGRLHPWPRSWKGAVE TGVRN in CAA51229 (PubMed:8353127).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72677 Genomic DNA. Translation: CAA51229.1. Different termination.
U28377 Genomic DNA. Translation: AAA69100.1.
U00096 Genomic DNA. Translation: AAC75970.1.
AP009048 Genomic DNA. Translation: BAE76996.1.
PIRiD65078. S36123.
RefSeqiNP_417408.1. NC_000913.3.
WP_000428805.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75970; AAC75970; b2933.
BAE76996; BAE76996; BAE76996.
GeneIDi945256.
KEGGiecj:JW2900.
eco:b2933.
PATRICi32121278. VBIEscCol129921_3027.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72677 Genomic DNA. Translation: CAA51229.1. Different termination.
U28377 Genomic DNA. Translation: AAA69100.1.
U00096 Genomic DNA. Translation: AAC75970.1.
AP009048 Genomic DNA. Translation: BAE76996.1.
PIRiD65078. S36123.
RefSeqiNP_417408.1. NC_000913.3.
WP_000428805.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP69826.
SMRiP69826. Positions 368-462.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262068. 76 interactions.
IntActiP69826. 3 interactions.
STRINGi511145.b2933.

Protein family/group databases

TCDBi4.A.2.1.24. the pts fructose-mannitol (fru) family.

Proteomic databases

PaxDbiP69826.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75970; AAC75970; b2933.
BAE76996; BAE76996; BAE76996.
GeneIDi945256.
KEGGiecj:JW2900.
eco:b2933.
PATRICi32121278. VBIEscCol129921_3027.

Organism-specific databases

EchoBASEiEB1740.
EcoGeneiEG11792. cmtA.

Phylogenomic databases

eggNOGiENOG4105CTS. Bacteria.
COG2213. LUCA.
HOGENOMiHOG000252814.
KOiK02799.
K02800.
OMAiDIVVTHE.
OrthoDBiEOG6FBWWQ.
PhylomeDBiP69826.

Enzyme and pathway databases

BioCyciEcoCyc:CMTA-MONOMER.
ECOL316407:JW2900-MONOMER.
MetaCyc:CMTA-MONOMER.

Miscellaneous databases

PROiP69826.

Family and domain databases

Gene3Di3.40.50.10370. 1 hit.
InterProiIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR029503. PTS_EIIB_mannitol.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR004718. PTS_IIC_mtl.
[Graphical view]
PfamiPF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
TIGRFAMsiTIGR00851. mtlA. 1 hit.
PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two open reading frames adjacent to the Escherichia coli K-12 transketolase (tkt) gene show high similarity to the mannitol phosphotransferase system enzymes from Escherichia coli and various Gram-positive bacteria."
    Sprenger G.A.
    Biochim. Biophys. Acta 1158:103-106(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-446.
    Strain: K12.
  2. Rudd K.E.
    Unpublished observations (JUN-1993)
    Cited for: PRESENCE OF VECTOR SEQUENCE CONTAMINATION IN SEQUENCE DESCRIBED IN PUBMED:8353127.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiPTMCB_ECOLI
AccessioniPrimary (citable) accession number: P69826
Secondary accession number(s): P32059, Q2M9R0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: May 11, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.