Reviewed, UniProtKB/Swiss-Prot P69826 (PTMCB_ECOLI)

Names and origin

Protein names

Recommended name:
    PTS system mannitol-specific cryptic EIICB component
Alternative name(s):
    EIICB-Mtl
      Short name=EII-Mtl
Including the following 2 domains:
    1- Recommended name:
            Mannitol permease IIC component
        Alternative name(s):
            PTS system mannitol-specific EIIC component
    2- Recommended name:
            Mannitol-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system mannitol-specific EIIB component

Gene names

Name:	cmtA
Ordered Locus Names:	b2933, JW2900

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell inner membrane; Multi-pass membrane protein. Ref.5
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-2 domain. Contains 1 PTS EIIC type-2 domain.
Sequence caution	The sequence CAA51229.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Vector contamination at the C-terminus.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
tsr	P02942	1	EBI-1131045,EBI-560870

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	PTS system mannitol-specific cryptic EIICB component		PRO_0000186679
Regions
	Topological domain	1 – 25	25	Cytoplasmic Potential
	Transmembrane	26 – 45	20	Probable
	Topological domain	46 – 51	6	Periplasmic Potential
	Transmembrane	52 – 70	19	Probable
	Topological domain	71 – 135	65	Cytoplasmic Potential
	Transmembrane	136 – 155	20	Probable
	Topological domain	156 – 166	11	Periplasmic Potential
	Transmembrane	167 – 185	19	Probable
	Topological domain	186 – 273	88	Cytoplasmic Potential
	Transmembrane	274 – 294	21	Probable
	Topological domain	295 – 316	22	Periplasmic Potential
	Transmembrane	317 – 336	20	Probable
	Topological domain	337 – 462	126	Cytoplasmic Potential
	Domain	13 – 344	332	PTS EIIC type-2
	Domain	371 – 461	91	PTS EIIB type-2
Sites
	Active site	377	1	Phosphocysteine intermediate; for EIIB activity By similarity
Experimental info
	Sequence conflict	274 – 275	2	MI → IEF in CAA51229. Ref.1
	Sequence conflict	415 – 435	21	DADIV…RVTDK → GCRISGRLHPWPRSWKGAVE TGVRN in CAA51229. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P69826-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 83F2D203D9E5A4C4	FASTA	462	48,971
	10 20 30 40 50 60 MENKSARAKV QAFGGFLTAM VIPNIGAFIA WGFITALFIP TGWLPNEHFA KIVGPMITYL 70 80 90 100 110 120 LPVMIGSTGG HLVGGKRGAV MGGIGTIGVI VGAEIPMFLG SMIMGPLGGL VIKYVDKALE 130 140 150 160 170 180 KRIPAGFEMV INNFSLGIAG MLLCLLGFEV IGPAVLIANT FVKECIEALV HAGYLPLLSV 190 200 210 220 230 240 INEPAKVLFL NNAIDQGVYY PLGMQQASVN GKSIFFMVAS NPGPGLGLLL AFTLFGKGMS 250 260 270 280 290 300 KRSAPGAMII HFLGGIHELY FPYVLMKPLT IIAMIAGGMS GTWMFNLLDG GLVAGPSPGS 310 320 330 340 350 360 IFAYLALTPK GSFLATIAGV TVGTLVSFAI TSLILKMEKT VETESEDEFA QSANAVKAMK 370 380 390 400 410 420 QEGAFSLSRV KRIAFVCDAG MGSSAMGATT FRKRLEKAGL AIEVKHYAIE NVPADADIVV 430 440 450 460 THASLEGRVK RVTDKPLILI NNYIGDPKLD TLFNQLTAEH KH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two open reading frames adjacent to the Escherichia coli K-12 transketolase (tkt) gene show high similarity to the mannitol phosphotransferase system enzymes from Escherichia coli and various Gram-positive bacteria." Sprenger G.A. Biochim. Biophys. Acta 1158:103-106(1993) [PubMed: 8353127] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-446. Strain: K12.
[2]	Rudd K.E. Unpublished observations (JUN-1993) Cited for: PRESENCE OF VECTOR SEQUENCE CONTAMINATION IN SEQUENCE DESCRIBED IN PUBMED:8353127.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X72677 Genomic DNA. Translation: CAA51229.1. Different termination. U28377 Genomic DNA. Translation: AAA69100.1. U00096 Genomic DNA. Translation: AAC75970.1. AP009048 Genomic DNA. Translation: BAE76996.1.
PIR	S36123. D65078.
RefSeq	AP_003490.1. NP_417408.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P69826. 3 interactions.
Genome annotation databases
GeneID	945256.
GenomeReviews	Gene locus JW2900 in contig AP009048_GR. Gene locus b2933 in contig U00096_GR.
KEGG	ecj:JW2900. eco:b2933.
Organism-specific databases
EchoBASE	EB1740.
EcoGene	EG11792. cmtA.
CMR	Search...
Phylogenomic databases
HOGENOM	P69826.
OMA	P69826. DQGIYYP.
Enzyme and pathway databases
BioCyc	EcoCyc:CMTA-MON.
Family and domain databases
InterPro	IPR013011. PTS_EIIB_2. IPR003352. PTS_EIIC. IPR013014. PTS_EIIC_2. IPR003501. PTS_IIB_lac. IPR004718. PTS_IIC_mtl. [Graphical view]
Pfam	PF02378. PTS_EIIC. 1 hit. PF02302. PTS_IIB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00851. mtlA. 1 hit.
PROSITE	PS51099. PTS_EIIB_TYPE_2. 1 hit. PS51104. PTS_EIIC_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTMCB_ECOLI

Accession

Primary (citable) accession number: P69826
Secondary accession number(s): P32059, Q2M9R0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 10, 2005
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)