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Protein

PTS system mannitol-specific cryptic EIICB component

Gene

cmtA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II CmtAB PTS system is involved in D-mannitol transport.By similarity

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-mannitol(Side 1) = [protein]-L-histidine + D-mannitol 1-phosphate(Side 2).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei377Phosphocysteine intermediate; for EIIB activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:CMTA-MONOMER.
ECOL316407:JW2900-MONOMER.
MetaCyc:CMTA-MONOMER.

Protein family/group databases

TCDBi4.A.2.1.24. the pts fructose-mannitol (fru) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system mannitol-specific cryptic EIICB component1 Publication
Alternative name(s):
EIICB-MtlBy similarity
Short name:
EII-MtlBy similarity
Including the following 2 domains:
Mannitol permease IIC componentBy similarity
Alternative name(s):
PTS system mannitol-specific EIIC componentBy similarity
Mannitol-specific phosphotransferase enzyme IIB componentBy similarity (EC:2.7.1.197By similarity)
Alternative name(s):
PTS system mannitol-specific EIIB componentBy similarity
Gene namesi
Name:cmtA1 Publication
Ordered Locus Names:b2933, JW2900
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11792. cmtA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 24CytoplasmicBy similarityAdd BLAST24
Transmembranei25 – 46HelicalBy similarityAdd BLAST22
Topological domaini47 – 50PeriplasmicBy similarity4
Transmembranei51 – 71HelicalBy similarityAdd BLAST21
Topological domaini72 – 134CytoplasmicBy similarityAdd BLAST63
Transmembranei135 – 156HelicalBy similarityAdd BLAST22
Topological domaini157 – 165PeriplasmicBy similarity9
Transmembranei166 – 186HelicalBy similarityAdd BLAST21
Topological domaini187 – 273CytoplasmicBy similarityAdd BLAST87
Transmembranei274 – 293HelicalBy similarityAdd BLAST20
Topological domaini294 – 313PeriplasmicBy similarityAdd BLAST20
Transmembranei314 – 335HelicalBy similarityAdd BLAST22
Topological domaini336 – 462CytoplasmicBy similarityAdd BLAST127

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001866791 – 462PTS system mannitol-specific cryptic EIICB componentAdd BLAST462

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei377Phosphocysteine; by EIIAPROSITE-ProRule annotationBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP69826.
PRIDEiP69826.

Interactioni

Protein-protein interaction databases

BioGridi4262068. 76 interactors.
IntActiP69826. 3 interactors.
STRINGi511145.b2933.

Structurei

3D structure databases

ProteinModelPortaliP69826.
SMRiP69826.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 344PTS EIIC type-2PROSITE-ProRule annotationAdd BLAST332
Domaini371 – 461PTS EIIB type-2PROSITE-ProRule annotationAdd BLAST91

Domaini

The EIIC type-2 domain forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation
The PTS EIIB type-2 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-2 domain.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 PTS EIIB type-2 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIC type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CTS. Bacteria.
COG2213. LUCA.
HOGENOMiHOG000252814.
KOiK02799.
K02800.
OMAiDIVVTHE.
PhylomeDBiP69826.

Family and domain databases

CDDicd05567. PTS_IIB_mannitol. 1 hit.
Gene3Di3.40.50.10370. 1 hit.
InterProiIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR029503. PTS_EIIB_mannitol.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR004718. PTS_IIC_mtl.
[Graphical view]
PfamiPF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
TIGRFAMsiTIGR00851. mtlA. 1 hit.
PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69826-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENKSARAKV QAFGGFLTAM VIPNIGAFIA WGFITALFIP TGWLPNEHFA
60 70 80 90 100
KIVGPMITYL LPVMIGSTGG HLVGGKRGAV MGGIGTIGVI VGAEIPMFLG
110 120 130 140 150
SMIMGPLGGL VIKYVDKALE KRIPAGFEMV INNFSLGIAG MLLCLLGFEV
160 170 180 190 200
IGPAVLIANT FVKECIEALV HAGYLPLLSV INEPAKVLFL NNAIDQGVYY
210 220 230 240 250
PLGMQQASVN GKSIFFMVAS NPGPGLGLLL AFTLFGKGMS KRSAPGAMII
260 270 280 290 300
HFLGGIHELY FPYVLMKPLT IIAMIAGGMS GTWMFNLLDG GLVAGPSPGS
310 320 330 340 350
IFAYLALTPK GSFLATIAGV TVGTLVSFAI TSLILKMEKT VETESEDEFA
360 370 380 390 400
QSANAVKAMK QEGAFSLSRV KRIAFVCDAG MGSSAMGATT FRKRLEKAGL
410 420 430 440 450
AIEVKHYAIE NVPADADIVV THASLEGRVK RVTDKPLILI NNYIGDPKLD
460
TLFNQLTAEH KH
Length:462
Mass (Da):48,971
Last modified:May 10, 2005 - v1
Checksum:i83F2D203D9E5A4C4
GO

Sequence cautioni

The sequence CAA51229 differs from that shown. Contaminating sequence. Vector contamination at the C-terminus.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti274 – 275MI → IEF in CAA51229 (PubMed:8353127).Curated2
Sequence conflicti415 – 435DADIV…RVTDK → GCRISGRLHPWPRSWKGAVE TGVRN in CAA51229 (PubMed:8353127).CuratedAdd BLAST21

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72677 Genomic DNA. Translation: CAA51229.1. Different termination.
U28377 Genomic DNA. Translation: AAA69100.1.
U00096 Genomic DNA. Translation: AAC75970.1.
AP009048 Genomic DNA. Translation: BAE76996.1.
PIRiD65078. S36123.
RefSeqiNP_417408.1. NC_000913.3.
WP_000428805.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75970; AAC75970; b2933.
BAE76996; BAE76996; BAE76996.
GeneIDi945256.
KEGGiecj:JW2900.
eco:b2933.
PATRICi32121278. VBIEscCol129921_3027.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72677 Genomic DNA. Translation: CAA51229.1. Different termination.
U28377 Genomic DNA. Translation: AAA69100.1.
U00096 Genomic DNA. Translation: AAC75970.1.
AP009048 Genomic DNA. Translation: BAE76996.1.
PIRiD65078. S36123.
RefSeqiNP_417408.1. NC_000913.3.
WP_000428805.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP69826.
SMRiP69826.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262068. 76 interactors.
IntActiP69826. 3 interactors.
STRINGi511145.b2933.

Protein family/group databases

TCDBi4.A.2.1.24. the pts fructose-mannitol (fru) family.

Proteomic databases

PaxDbiP69826.
PRIDEiP69826.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75970; AAC75970; b2933.
BAE76996; BAE76996; BAE76996.
GeneIDi945256.
KEGGiecj:JW2900.
eco:b2933.
PATRICi32121278. VBIEscCol129921_3027.

Organism-specific databases

EchoBASEiEB1740.
EcoGeneiEG11792. cmtA.

Phylogenomic databases

eggNOGiENOG4105CTS. Bacteria.
COG2213. LUCA.
HOGENOMiHOG000252814.
KOiK02799.
K02800.
OMAiDIVVTHE.
PhylomeDBiP69826.

Enzyme and pathway databases

BioCyciEcoCyc:CMTA-MONOMER.
ECOL316407:JW2900-MONOMER.
MetaCyc:CMTA-MONOMER.

Miscellaneous databases

PROiP69826.

Family and domain databases

CDDicd05567. PTS_IIB_mannitol. 1 hit.
Gene3Di3.40.50.10370. 1 hit.
InterProiIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR029503. PTS_EIIB_mannitol.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR004718. PTS_IIC_mtl.
[Graphical view]
PfamiPF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
TIGRFAMsiTIGR00851. mtlA. 1 hit.
PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTMCB_ECOLI
AccessioniPrimary (citable) accession number: P69826
Secondary accession number(s): P32059, Q2M9R0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.