PTS system mannitol-specific cryptic EIICB component

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P69826 (PTMCB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 68. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
PTS system mannitol-specific cryptic EIICB component

Alternative name(s):
EIICB-Mtl
Short name=EII-Mtl

Including the following 2 domains:

Mannitol permease IIC component
Alternative name(s):
PTS system mannitol-specific EIIC component
Mannitol-specific phosphotransferase enzyme IIB component
EC=2.7.1.69
Alternative name(s):
PTS system mannitol-specific EIIB component

Gene names

Name:	cmtA
Ordered Locus Names:	b2933, JW2900

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.5.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-2 domain. Contains 1 PTS EIIC type-2 domain.
Sequence caution	The sequence CAA51229.1 differs from that shown. Reason: Contaminating sequence. Vector contamination at the C-terminus.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 462

462

PTS system mannitol-specific cryptic EIICB component

PRO_0000186679

Regions

Topological domain

1 – 25

Cytoplasmic Potential

Transmembrane

26 – 45

Helical; Probable

Topological domain

46 – 51

Periplasmic Potential

Transmembrane

52 – 70

Helical; Probable

Topological domain

71 – 135

Cytoplasmic Potential

Transmembrane

136 – 155

Helical; Probable

Topological domain

156 – 166

Periplasmic Potential

Transmembrane

167 – 185

Helical; Probable

Topological domain

186 – 273

Cytoplasmic Potential

Transmembrane

274 – 294

Helical; Probable

Topological domain

295 – 316

Periplasmic Potential

Transmembrane

317 – 336

Helical; Probable

Topological domain

337 – 462

126

Cytoplasmic Potential

Domain

13 – 344

332

PTS EIIC type-2

Domain

371 – 461

PTS EIIB type-2

Sites

Active site

377

Phosphocysteine intermediate; for EIIB activity By similarity

Experimental info

Sequence conflict

274 – 275

MI → IEF in CAA51229. Ref.1

Sequence conflict

415 – 435

DADIV…RVTDK → GCRISGRLHPWPRSWKGAVE TGVRN in CAA51229. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P69826 [UniParc].

Last modified May 10, 2005. Version 1.
Checksum: 83F2D203D9E5A4C4
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FASTA

462

48,971

        10         20         30         40         50         60 
MENKSARAKV QAFGGFLTAM VIPNIGAFIA WGFITALFIP TGWLPNEHFA KIVGPMITYL 

        70         80         90        100        110        120 
LPVMIGSTGG HLVGGKRGAV MGGIGTIGVI VGAEIPMFLG SMIMGPLGGL VIKYVDKALE 

       130        140        150        160        170        180 
KRIPAGFEMV INNFSLGIAG MLLCLLGFEV IGPAVLIANT FVKECIEALV HAGYLPLLSV 

       190        200        210        220        230        240 
INEPAKVLFL NNAIDQGVYY PLGMQQASVN GKSIFFMVAS NPGPGLGLLL AFTLFGKGMS 

       250        260        270        280        290        300 
KRSAPGAMII HFLGGIHELY FPYVLMKPLT IIAMIAGGMS GTWMFNLLDG GLVAGPSPGS 

       310        320        330        340        350        360 
IFAYLALTPK GSFLATIAGV TVGTLVSFAI TSLILKMEKT VETESEDEFA QSANAVKAMK 

       370        380        390        400        410        420 
QEGAFSLSRV KRIAFVCDAG MGSSAMGATT FRKRLEKAGL AIEVKHYAIE NVPADADIVV 

       430        440        450        460 
THASLEGRVK RVTDKPLILI NNYIGDPKLD TLFNQLTAEH KH

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two open reading frames adjacent to the Escherichia coli K-12 transketolase (tkt) gene show high similarity to the mannitol phosphotransferase system enzymes from Escherichia coli and various Gram-positive bacteria." Sprenger G.A. Biochim. Biophys. Acta 1158:103-106(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-446. Strain: K12.
[2]	Rudd K.E. Unpublished observations (JUN-1993) Cited for: PRESENCE OF VECTOR SEQUENCE CONTAMINATION IN SEQUENCE DESCRIBED IN PUBMED:8353127.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X72677 Genomic DNA. Translation: CAA51229.1. Different termination. U28377 Genomic DNA. Translation: AAA69100.1. U00096 Genomic DNA. Translation: AAC75970.1. AP009048 Genomic DNA. Translation: BAE76996.1.
PIR	S36123. D65078.
RefSeq	NP_417408.1. NC_000913.2. YP_491132.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P69826.
SMR	P69826. Positions 368-462.
ModBase	Search...
Protein-protein interaction databases
IntAct	P69826. 3 interactions.
STRING	511145.b2933.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75970; AAC75970; b2933. BAE76996; BAE76996; BAE76996.
GeneID	12933074. 945256.
KEGG	ecj:Y75_p2863. eco:b2933.
PATRIC	32121278. VBIEscCol129921_3027.
Organism-specific databases
EchoBASE	EB1740.
EcoGene	EG11792. cmtA.
Phylogenomic databases
eggNOG	COG2213.
HOGENOM	HOG000252814.
KO	K02799. K02800.
OMA	MIVAMIA.
ProtClustDB	CLSK2460192.
Enzyme and pathway databases
BioCyc	EcoCyc:CMTA-MONOMER. ECOL316407:JW2900-MONOMER. MetaCyc:CMTA-MONOMER.
Gene expression databases
Genevestigator	P69826.
Family and domain databases
Gene3D	3.40.50.10370. 1 hit.
InterPro	IPR013011. PTS_EIIB_2. IPR003501. PTS_EIIB_2/3. IPR003352. PTS_EIIC. IPR013014. PTS_EIIC_2. IPR004718. PTS_IIC_mtl. [Graphical view]
Pfam	PF02378. PTS_EIIC. 1 hit. PF02302. PTS_IIB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00851. mtlA. 1 hit.
PROSITE	PS51099. PTS_EIIB_TYPE_2. 1 hit. PS51104. PTS_EIIC_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTMCB_ECOLI

Accession

Primary (citable) accession number: P69826
Secondary accession number(s): P32059, Q2M9R0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 10, 2005
Last modified:	May 29, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Including the following 2 domains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents