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Protein

Mannitol-specific cryptic phosphotransferase enzyme IIA component

Gene

cmtB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport (By similarity).By similarity

Catalytic activityi

Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671Tele-phosphohistidine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:CMTB-MONOMER.
ECOL316407:JW2901-MONOMER.
MetaCyc:CMTB-MONOMER.

Protein family/group databases

TCDBi4.A.2.1.24. the pts fructose-mannitol (fru) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannitol-specific cryptic phosphotransferase enzyme IIA component (EC:2.7.1.-)
Alternative name(s):
EIIA-Mtl
EIII-Mtl
PTS system mannitol-specific EIIA component
Gene namesi
Name:cmtB
Ordered Locus Names:b2934, JW2901
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11791. cmtB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 147147Mannitol-specific cryptic phosphotransferase enzyme IIA componentPRO_0000186681Add
BLAST

Proteomic databases

PaxDbiP69824.

Interactioni

Protein-protein interaction databases

BioGridi4261871. 15 interactions.
IntActiP69824. 2 interactions.
STRINGi511145.b2934.

Structurei

Secondary structure

1
147
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 63Combined sources
Beta strandi12 – 165Combined sources
Helixi21 – 277Combined sources
Helixi30 – 334Combined sources
Turni34 – 363Combined sources
Helixi40 – 5213Combined sources
Beta strandi58 – 603Combined sources
Helixi70 – 723Combined sources
Beta strandi78 – 8811Combined sources
Beta strandi97 – 1048Combined sources
Helixi108 – 12215Combined sources
Helixi125 – 1328Combined sources
Helixi137 – 1459Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OQ3NMR-A1-147[»]
ProteinModelPortaliP69824.
SMRiP69824. Positions 1-147.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69824.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 147143PTS EIIA type-2PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.

Sequence similaritiesi

Contains 1 PTS EIIA type-2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108X7Y. Bacteria.
COG1762. LUCA.
HOGENOMiHOG000227560.
KOiK02798.
OMAiYKYGPYI.
PhylomeDBiP69824.

Family and domain databases

Gene3Di3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
[Graphical view]
SUPFAMiSSF55804. SSF55804. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69824-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLSDYFPES SISVIHSAKD WQEAIDFSMV SLLDKNYISE NYIQAIKDST
60 70 80 90 100
INNGPYYILA PGVAMPHARP ECGALKTGMS LTLLEQGVYF PGNDEPIKLL
110 120 130 140
IGLSAADADS HIGAIQALSE LLCEEEILEQ LLTASSEKQL ADIISRG
Length:147
Mass (Da):16,046
Last modified:May 10, 2005 - v1
Checksum:i4E09A4E6D88D9190
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72677 Genomic DNA. Translation: CAA51228.1.
U28377 Genomic DNA. Translation: AAA69101.1.
U00096 Genomic DNA. Translation: AAC75971.1.
AP009048 Genomic DNA. Translation: BAE76997.1.
PIRiS36122.
RefSeqiNP_417409.1. NC_000913.3.
WP_001239650.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75971; AAC75971; b2934.
BAE76997; BAE76997; BAE76997.
GeneIDi945125.
KEGGiecj:JW2901.
eco:b2934.
PATRICi32121280. VBIEscCol129921_3028.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72677 Genomic DNA. Translation: CAA51228.1.
U28377 Genomic DNA. Translation: AAA69101.1.
U00096 Genomic DNA. Translation: AAC75971.1.
AP009048 Genomic DNA. Translation: BAE76997.1.
PIRiS36122.
RefSeqiNP_417409.1. NC_000913.3.
WP_001239650.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OQ3NMR-A1-147[»]
ProteinModelPortaliP69824.
SMRiP69824. Positions 1-147.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261871. 15 interactions.
IntActiP69824. 2 interactions.
STRINGi511145.b2934.

Protein family/group databases

TCDBi4.A.2.1.24. the pts fructose-mannitol (fru) family.

Proteomic databases

PaxDbiP69824.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75971; AAC75971; b2934.
BAE76997; BAE76997; BAE76997.
GeneIDi945125.
KEGGiecj:JW2901.
eco:b2934.
PATRICi32121280. VBIEscCol129921_3028.

Organism-specific databases

EchoBASEiEB1739.
EcoGeneiEG11791. cmtB.

Phylogenomic databases

eggNOGiENOG4108X7Y. Bacteria.
COG1762. LUCA.
HOGENOMiHOG000227560.
KOiK02798.
OMAiYKYGPYI.
PhylomeDBiP69824.

Enzyme and pathway databases

BioCyciEcoCyc:CMTB-MONOMER.
ECOL316407:JW2901-MONOMER.
MetaCyc:CMTB-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP69824.
PROiP69824.

Family and domain databases

Gene3Di3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
[Graphical view]
SUPFAMiSSF55804. SSF55804. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTMA_ECOLI
AccessioniPrimary (citable) accession number: P69824
Secondary accession number(s): P32058, Q2M9Q9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: September 7, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.