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Protein

Ascorbate-specific PTS system EIIB component

Gene

ulaB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II UlaABC PTS system is involved in ascorbate transport.1 Publication1 Publication

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + L-ascorbate(Side 1) = [protein]-L-histidine + L-ascorbate 6-phosphate(Side 2).1 Publication

Kineticsi

  1. KM=18 µM for L-ascorbate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei9Phosphocysteine intermediateBy similarityCurated1

    GO - Molecular functioni

    GO - Biological processi

    • L-ascorbic acid transmembrane transport Source: EcoCyc
    • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc

    Keywordsi

    Molecular functionKinase, Transferase
    Biological processPhosphotransferase system, Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:YJFT-MONOMER
    MetaCyc:YJFT-MONOMER

    Protein family/group databases

    TCDBi4.A.7.1.1 the pts l-ascorbate (l-asc) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ascorbate-specific PTS system EIIB component1 Publication (EC:2.7.1.1941 Publication)
    Alternative name(s):
    Ascorbate-specific phosphotransferase enzyme IIB component1 Publication
    Gene namesi
    Name:ulaB1 Publication
    Synonyms:sgaB, yjfT
    Ordered Locus Names:b4194, JW4152
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG12494 ulaB

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene are unable to use L-ascorbate.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001866861 – 101Ascorbate-specific PTS system EIIB componentAdd BLAST101

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei9PhosphocysteineBy similarityCurated1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP69822
    PRIDEiP69822

    Expressioni

    Inductioni

    Induced by L-ascorbate. Repressed by UlaR.3 Publications

    Interactioni

    Protein-protein interaction databases

    BioGridi4262714, 8 interactors
    IntActiP69822, 5 interactors
    STRINGi316385.ECDH10B_4389

    Structurei

    3D structure databases

    ProteinModelPortaliP69822
    SMRiP69822
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini1 – 100PTS EIIB type-2PROSITE-ProRule annotationAdd BLAST100

    Domaini

    The PTS EIIB type-2 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-2 domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiENOG410907W Bacteria
    COG3414 LUCA
    HOGENOMiHOG000081922
    KOiK02822
    OMAiMDVNEIK

    Family and domain databases

    InterProiView protein in InterPro
    IPR036095 PTS_EIIB-like_sf
    IPR013011 PTS_EIIB_2
    IPR003501 PTS_EIIB_2/3
    PfamiView protein in Pfam
    PF02302 PTS_IIB, 1 hit
    SUPFAMiSSF52794 SSF52794, 1 hit
    PROSITEiView protein in PROSITE
    PS51099 PTS_EIIB_TYPE_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P69822-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTVRILAVCG NGQGSSMIMK MKVDQFLTQS NIDHTVNSCA VGEYKSELSG
    60 70 80 90 100
    ADIIIASTHI AGEITVTGNK YVVGVRNMLS PADFGPKLLE VIKEHFPQDV

    K
    Length:101
    Mass (Da):10,896
    Last modified:May 10, 2005 - v1
    Checksum:i84CFB6B17250D47D
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U14003 Genomic DNA Translation: AAA97090.1
    U00096 Genomic DNA Translation: AAC77151.1
    AP009048 Genomic DNA Translation: BAE78195.1
    PIRiS56419
    RefSeqiNP_418615.1, NC_000913.3
    WP_000218362.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC77151; AAC77151; b4194
    BAE78195; BAE78195; BAE78195
    GeneIDi948716
    KEGGiecj:JW4152
    eco:b4194
    PATRICifig|1411691.4.peg.2507

    Entry informationi

    Entry nameiULAB_ECOLI
    AccessioniPrimary (citable) accession number: P69822
    Secondary accession number(s): P39302, Q2M6B1
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
    Last sequence update: May 10, 2005
    Last modified: April 25, 2018
    This is version 98 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
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