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Protein

Ascorbate-specific phosphotransferase enzyme IIB component

Gene

ulaB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in ascorbate transport.

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + L-ascorbate(Side 1) = [protein]-L-histidine + L-ascorbate 6-phosphate(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei9 – 91Phosphocysteine intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

  • carbohydrate transmembrane transport Source: GOC
  • L-ascorbic acid transport Source: EcoCyc
  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YJFT-MONOMER.
ECOL316407:JW4152-MONOMER.
MetaCyc:YJFT-MONOMER.

Protein family/group databases

TCDBi4.A.7.1.1. the pts l-ascorbate (l-asc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ascorbate-specific phosphotransferase enzyme IIB component (EC:2.7.1.194)
Alternative name(s):
Ascorbate-specific PTS system EIIB component
Gene namesi
Name:ulaB
Synonyms:sgaB, yjfT
Ordered Locus Names:b4194, JW4152
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12494. ulaB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 101101Ascorbate-specific phosphotransferase enzyme IIB componentPRO_0000186686Add
BLAST

Proteomic databases

PaxDbiP69822.

Expressioni

Inductioni

Induced by L-ascorbate. Repressed by UlaR.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4262714. 8 interactions.
IntActiP69822. 5 interactions.
STRINGi511145.b4194.

Structurei

3D structure databases

ProteinModelPortaliP69822.
SMRiP69822. Positions 3-93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 100100PTS EIIB type-2PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

Sequence similaritiesi

Contains 1 PTS EIIB type-2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410907W. Bacteria.
COG3414. LUCA.
HOGENOMiHOG000081922.
KOiK02822.
OMAiIVASTHV.
OrthoDBiEOG6J48VN.

Family and domain databases

InterProiIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
[Graphical view]
PfamiPF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69822-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVRILAVCG NGQGSSMIMK MKVDQFLTQS NIDHTVNSCA VGEYKSELSG
60 70 80 90 100
ADIIIASTHI AGEITVTGNK YVVGVRNMLS PADFGPKLLE VIKEHFPQDV

K
Length:101
Mass (Da):10,896
Last modified:May 10, 2005 - v1
Checksum:i84CFB6B17250D47D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97090.1.
U00096 Genomic DNA. Translation: AAC77151.1.
AP009048 Genomic DNA. Translation: BAE78195.1.
PIRiS56419.
RefSeqiNP_418615.1. NC_000913.3.
WP_000218362.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77151; AAC77151; b4194.
BAE78195; BAE78195; BAE78195.
GeneIDi948716.
KEGGiecj:JW4152.
eco:b4194.
PATRICi32123963. VBIEscCol129921_4326.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97090.1.
U00096 Genomic DNA. Translation: AAC77151.1.
AP009048 Genomic DNA. Translation: BAE78195.1.
PIRiS56419.
RefSeqiNP_418615.1. NC_000913.3.
WP_000218362.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP69822.
SMRiP69822. Positions 3-93.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262714. 8 interactions.
IntActiP69822. 5 interactions.
STRINGi511145.b4194.

Protein family/group databases

TCDBi4.A.7.1.1. the pts l-ascorbate (l-asc) family.

Proteomic databases

PaxDbiP69822.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77151; AAC77151; b4194.
BAE78195; BAE78195; BAE78195.
GeneIDi948716.
KEGGiecj:JW4152.
eco:b4194.
PATRICi32123963. VBIEscCol129921_4326.

Organism-specific databases

EchoBASEiEB2387.
EcoGeneiEG12494. ulaB.

Phylogenomic databases

eggNOGiENOG410907W. Bacteria.
COG3414. LUCA.
HOGENOMiHOG000081922.
KOiK02822.
OMAiIVASTHV.
OrthoDBiEOG6J48VN.

Enzyme and pathway databases

BioCyciEcoCyc:YJFT-MONOMER.
ECOL316407:JW4152-MONOMER.
MetaCyc:YJFT-MONOMER.

Miscellaneous databases

PROiP69822.

Family and domain databases

InterProiIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
[Graphical view]
PfamiPF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Novel phosphotransferases system genes revealed by bacterial genome analysis: operons encoding homologues of sugar-specific permease domains of the phosphotransferase system and pentose catabolic enzymes."
    Reizer J., Charbit A., Reizer A., Saier M.H. Jr.
    Genome Sci. Technol. 1:53-75(1996)
    Cited for: DISCUSSION OF SEQUENCE.
  5. "The gene yjfQ encodes the repressor of the yjfR-X regulon (ula), which is involved in L-ascorbate metabolism in Escherichia coli."
    Campos E., Aguilar J., Baldoma L., Badia J.
    J. Bacteriol. 184:6065-6068(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TRANSCRIPTIONAL REGULATION.
  6. Cited for: CHARACTERIZATION.
  7. "Regulation of expression of the divergent ulaG and ulaABCDEF operons involved in L-ascorbate dissimilation in Escherichia coli."
    Campos E., Baldoma L., Aguilar J., Badia J.
    J. Bacteriol. 186:1720-1728(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TRANSCRIPTIONAL REGULATION.

Entry informationi

Entry nameiULAB_ECOLI
AccessioniPrimary (citable) accession number: P69822
Secondary accession number(s): P39302, Q2M6B1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: June 8, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.