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Protein

Ascorbate-specific PTS system EIIB component

Gene

ulaB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II UlaABC PTS system is involved in ascorbate transport.1 Publication1 Publication

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + L-ascorbate(Side 1) = [protein]-L-histidine + L-ascorbate 6-phosphate(Side 2).1 Publication

Kineticsi

  1. KM=18 µM for L-ascorbate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei9 – 91Phosphocysteine intermediateBy similarityCurated

    GO - Molecular functioni

    GO - Biological processi

    • L-ascorbic acid transport Source: EcoCyc
    • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Phosphotransferase system, Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:YJFT-MONOMER.
    ECOL316407:JW4152-MONOMER.
    MetaCyc:YJFT-MONOMER.

    Protein family/group databases

    TCDBi4.A.7.1.1. the pts l-ascorbate (l-asc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ascorbate-specific PTS system EIIB component1 Publication (EC:2.7.1.1941 Publication)
    Alternative name(s):
    Ascorbate-specific phosphotransferase enzyme IIB component1 Publication
    Gene namesi
    Name:ulaB1 Publication
    Synonyms:sgaB, yjfT
    Ordered Locus Names:b4194, JW4152
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG12494. ulaB.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene are unable to use L-ascorbate.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 101101Ascorbate-specific PTS system EIIB componentPRO_0000186686Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei9 – 91PhosphocysteineBy similarityCurated

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP69822.

    Expressioni

    Inductioni

    Induced by L-ascorbate. Repressed by UlaR.3 Publications

    Interactioni

    Protein-protein interaction databases

    BioGridi4262714. 8 interactions.
    IntActiP69822. 5 interactions.
    STRINGi511145.b4194.

    Structurei

    3D structure databases

    ProteinModelPortaliP69822.
    SMRiP69822. Positions 3-93.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 100100PTS EIIB type-2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The PTS EIIB type-2 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-2 domain.PROSITE-ProRule annotation

    Sequence similaritiesi

    Contains 1 PTS EIIB type-2 domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiENOG410907W. Bacteria.
    COG3414. LUCA.
    HOGENOMiHOG000081922.
    KOiK02822.
    OMAiIVASTHV.

    Family and domain databases

    InterProiIPR013011. PTS_EIIB_2.
    IPR003501. PTS_EIIB_2/3.
    [Graphical view]
    PfamiPF02302. PTS_IIB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52794. SSF52794. 1 hit.
    PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P69822-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTVRILAVCG NGQGSSMIMK MKVDQFLTQS NIDHTVNSCA VGEYKSELSG
    60 70 80 90 100
    ADIIIASTHI AGEITVTGNK YVVGVRNMLS PADFGPKLLE VIKEHFPQDV

    K
    Length:101
    Mass (Da):10,896
    Last modified:May 10, 2005 - v1
    Checksum:i84CFB6B17250D47D
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U14003 Genomic DNA. Translation: AAA97090.1.
    U00096 Genomic DNA. Translation: AAC77151.1.
    AP009048 Genomic DNA. Translation: BAE78195.1.
    PIRiS56419.
    RefSeqiNP_418615.1. NC_000913.3.
    WP_000218362.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77151; AAC77151; b4194.
    BAE78195; BAE78195; BAE78195.
    GeneIDi948716.
    KEGGiecj:JW4152.
    eco:b4194.
    PATRICi32123963. VBIEscCol129921_4326.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U14003 Genomic DNA. Translation: AAA97090.1.
    U00096 Genomic DNA. Translation: AAC77151.1.
    AP009048 Genomic DNA. Translation: BAE78195.1.
    PIRiS56419.
    RefSeqiNP_418615.1. NC_000913.3.
    WP_000218362.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP69822.
    SMRiP69822. Positions 3-93.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4262714. 8 interactions.
    IntActiP69822. 5 interactions.
    STRINGi511145.b4194.

    Protein family/group databases

    TCDBi4.A.7.1.1. the pts l-ascorbate (l-asc) family.

    Proteomic databases

    PaxDbiP69822.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC77151; AAC77151; b4194.
    BAE78195; BAE78195; BAE78195.
    GeneIDi948716.
    KEGGiecj:JW4152.
    eco:b4194.
    PATRICi32123963. VBIEscCol129921_4326.

    Organism-specific databases

    EchoBASEiEB2387.
    EcoGeneiEG12494. ulaB.

    Phylogenomic databases

    eggNOGiENOG410907W. Bacteria.
    COG3414. LUCA.
    HOGENOMiHOG000081922.
    KOiK02822.
    OMAiIVASTHV.

    Enzyme and pathway databases

    BioCyciEcoCyc:YJFT-MONOMER.
    ECOL316407:JW4152-MONOMER.
    MetaCyc:YJFT-MONOMER.

    Miscellaneous databases

    PROiP69822.

    Family and domain databases

    InterProiIPR013011. PTS_EIIB_2.
    IPR003501. PTS_EIIB_2/3.
    [Graphical view]
    PfamiPF02302. PTS_IIB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52794. SSF52794. 1 hit.
    PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiULAB_ECOLI
    AccessioniPrimary (citable) accession number: P69822
    Secondary accession number(s): P39302, Q2M6B1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2005
    Last sequence update: May 10, 2005
    Last modified: September 7, 2016
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.