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Protein

Ascorbate-specific PTS system EIIA component

Gene

ulaC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II UlaABC PTS system is involved in ascorbate transport.1 Publication1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei68Tele-phosphohistidine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • kinase activity Source: UniProtKB-KW
  • protein-phosphocysteine-L-ascorbate-phosphotransferase system transporter activity Source: EcoCyc

GO - Biological processi

  • L-ascorbic acid transmembrane transport Source: EcoCyc
  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphotransferase system, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YJFU-MONOMER
MetaCyc:YJFU-MONOMER

Protein family/group databases

TCDBi4.A.7.1.1 the pts l-ascorbate (l-asc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Ascorbate-specific PTS system EIIA component1 Publication
Alternative name(s):
Ascorbate-specific phosphotransferase enzyme IIA component1 Publication
Gene namesi
Name:ulaC1 Publication
Synonyms:ptxA, sgaA, yjfU
Ordered Locus Names:b4195, JW4153
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12495 ulaC

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene are unable to use L-ascorbate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001866831 – 154Ascorbate-specific PTS system EIIA componentAdd BLAST154

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei68PhosphohistidineCurated1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP69820
PRIDEiP69820

Expressioni

Inductioni

Induced by L-ascorbate. Repressed by UlaR.3 Publications

Interactioni

Protein-protein interaction databases

BioGridi4262708, 9 interactors
IntActiP69820, 1 interactor
STRINGi316385.ECDH10B_4390

Structurei

3D structure databases

ProteinModelPortaliP69820
SMRiP69820
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 150PTS EIIA type-2PROSITE-ProRule annotationAdd BLAST145

Domaini

The PTS EIIA type-2 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4106ETB Bacteria
COG1762 LUCA
HOGENOMiHOG000227560
InParanoidiP69820
KOiK02821
OMAiVFNHEDN
PhylomeDBiP69820

Family and domain databases

Gene3Di3.40.930.10, 1 hit
InterProiView protein in InterPro
IPR016152 PTrfase/Anion_transptr
IPR002178 PTS_EIIA_type-2_dom
PfamiView protein in Pfam
PF00359 PTS_EIIA_2, 1 hit
SUPFAMiSSF55804 SSF55804, 1 hit
PROSITEiView protein in PROSITE
PS51094 PTS_EIIA_TYPE_2, 1 hit
PS00372 PTS_EIIA_TYPE_2_HIS, 1 hit

Sequencei

Sequence statusi: Complete.

P69820-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLRDSLAEN KSIRLQAEAE TWQEAVKIGV DLLVAADVVE PRYYQAILDG
60 70 80 90 100
VEQFGPYFVI APGLAMPHGR PEEGVKKTGF SLVTLKKPLE FNHDDNDPVD
110 120 130 140 150
ILITMAAVDA NTHQEVGIMQ IVNLFEDEEN FDRLRACRTE QEVLDLIDRT

NAAA
Length:154
Mass (Da):17,238
Last modified:May 10, 2005 - v1
Checksum:iBE83931C5A98DB79
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti153 – 154AA → GSLRRN in AAA97091 (PubMed:7610040).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA Translation: AAA97091.1
U00096 Genomic DNA Translation: AAC77152.1
AP009048 Genomic DNA Translation: BAE78196.1
PIRiF65230
RefSeqiNP_418616.1, NC_000913.3
WP_000776517.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77152; AAC77152; b4195
BAE78196; BAE78196; BAE78196
GeneIDi948715
KEGGiecj:JW4153
eco:b4195
PATRICifig|1411691.4.peg.2506

Similar proteinsi

Entry informationi

Entry nameiULAC_ECOLI
AccessioniPrimary (citable) accession number: P69820
Secondary accession number(s): P39303, Q2M6B0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: April 25, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health