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Protein

Ascorbate-specific phosphotransferase enzyme IIA component

Gene

ulaC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in ascorbate transport.

Catalytic activityi

Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei68 – 681Tele-phosphohistidine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  • kinase activity Source: UniProtKB-KW
  • protein-phosphocysteine-L-ascorbate-phosphotransferase system transporter activity Source: EcoCyc

GO - Biological processi

  • L-ascorbic acid transport Source: EcoCyc
  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YJFU-MONOMER.
ECOL316407:JW4153-MONOMER.
MetaCyc:YJFU-MONOMER.

Protein family/group databases

TCDBi4.A.7.1.1. the pts l-ascorbate (l-asc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ascorbate-specific phosphotransferase enzyme IIA component (EC:2.7.1.-)
Alternative name(s):
PTS system ascorbate-specific EIIA component
Gene namesi
Name:ulaC
Synonyms:ptxA, sgaA, yjfU
Ordered Locus Names:b4195, JW4153
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12495. ulaC.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 154154Ascorbate-specific phosphotransferase enzyme IIA componentPRO_0000186683Add
BLAST

Proteomic databases

PaxDbiP69820.

Expressioni

Inductioni

Induced by L-ascorbate. Repressed by UlaR.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4262708. 9 interactions.
IntActiP69820. 1 interaction.
STRINGi511145.b4195.

Structurei

3D structure databases

ProteinModelPortaliP69820.
SMRiP69820. Positions 1-150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 150145PTS EIIA type-2PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.

Sequence similaritiesi

Contains 1 PTS EIIA type-2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4106ETB. Bacteria.
COG1762. LUCA.
HOGENOMiHOG000227560.
InParanoidiP69820.
KOiK02821.
OMAiVFNHEDN.
OrthoDBiEOG68DCVK.
PhylomeDBiP69820.

Family and domain databases

Gene3Di3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
[Graphical view]
SUPFAMiSSF55804. SSF55804. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69820-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLRDSLAEN KSIRLQAEAE TWQEAVKIGV DLLVAADVVE PRYYQAILDG
60 70 80 90 100
VEQFGPYFVI APGLAMPHGR PEEGVKKTGF SLVTLKKPLE FNHDDNDPVD
110 120 130 140 150
ILITMAAVDA NTHQEVGIMQ IVNLFEDEEN FDRLRACRTE QEVLDLIDRT

NAAA
Length:154
Mass (Da):17,238
Last modified:May 10, 2005 - v1
Checksum:iBE83931C5A98DB79
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti153 – 1542AA → GSLRRN in AAA97091 (PubMed:7610040).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97091.1.
U00096 Genomic DNA. Translation: AAC77152.1.
AP009048 Genomic DNA. Translation: BAE78196.1.
PIRiF65230.
RefSeqiNP_418616.1. NC_000913.3.
WP_000776517.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77152; AAC77152; b4195.
BAE78196; BAE78196; BAE78196.
GeneIDi948715.
KEGGiecj:JW4153.
eco:b4195.
PATRICi32123965. VBIEscCol129921_4327.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97091.1.
U00096 Genomic DNA. Translation: AAC77152.1.
AP009048 Genomic DNA. Translation: BAE78196.1.
PIRiF65230.
RefSeqiNP_418616.1. NC_000913.3.
WP_000776517.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP69820.
SMRiP69820. Positions 1-150.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262708. 9 interactions.
IntActiP69820. 1 interaction.
STRINGi511145.b4195.

Protein family/group databases

TCDBi4.A.7.1.1. the pts l-ascorbate (l-asc) family.

Proteomic databases

PaxDbiP69820.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77152; AAC77152; b4195.
BAE78196; BAE78196; BAE78196.
GeneIDi948715.
KEGGiecj:JW4153.
eco:b4195.
PATRICi32123965. VBIEscCol129921_4327.

Organism-specific databases

EchoBASEiEB2388.
EcoGeneiEG12495. ulaC.

Phylogenomic databases

eggNOGiENOG4106ETB. Bacteria.
COG1762. LUCA.
HOGENOMiHOG000227560.
InParanoidiP69820.
KOiK02821.
OMAiVFNHEDN.
OrthoDBiEOG68DCVK.
PhylomeDBiP69820.

Enzyme and pathway databases

BioCyciEcoCyc:YJFU-MONOMER.
ECOL316407:JW4153-MONOMER.
MetaCyc:YJFU-MONOMER.

Miscellaneous databases

PROiP69820.

Family and domain databases

Gene3Di3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
[Graphical view]
SUPFAMiSSF55804. SSF55804. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO C-TERMINUS.
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Novel phosphotransferases system genes revealed by bacterial genome analysis: operons encoding homologues of sugar-specific permease domains of the phosphotransferase system and pentose catabolic enzymes."
    Reizer J., Charbit A., Reizer A., Saier M.H. Jr.
    Genome Sci. Technol. 1:53-75(1996)
    Cited for: DISCUSSION OF SEQUENCE.
  5. "The gene yjfQ encodes the repressor of the yjfR-X regulon (ula), which is involved in L-ascorbate metabolism in Escherichia coli."
    Campos E., Aguilar J., Baldoma L., Badia J.
    J. Bacteriol. 184:6065-6068(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TRANSCRIPTIONAL REGULATION.
  6. Cited for: CHARACTERIZATION.
  7. "Regulation of expression of the divergent ulaG and ulaABCDEF operons involved in L-ascorbate dissimilation in Escherichia coli."
    Campos E., Baldoma L., Aguilar J., Badia J.
    J. Bacteriol. 186:1720-1728(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TRANSCRIPTIONAL REGULATION.

Entry informationi

Entry nameiULAC_ECOLI
AccessioniPrimary (citable) accession number: P69820
Secondary accession number(s): P39303, Q2M6B0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: July 6, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.