Reviewed, UniProtKB/Swiss-Prot P69819 (PTFB2_SHIFL)

Names and origin

Protein names

Recommended name:
    Fructose-like phosphotransferase enzyme IIB component 2
    EC=2.7.1.69
Alternative name(s):
    PTS system fructose-like EIIB component 2

Gene names

Name:	frwB
Ordered Locus Names:	SF4027, S3719

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	106 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cytoplasm.
Domain	The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-2 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 106	106	Fructose-like phosphotransferase enzyme IIB component 2		PRO_0000186504
Regions
	Domain	1 – 103	103	PTS EIIB type-2
Sites
	Active site	10	1	Phosphocysteine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P69819-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: F712189F18489D3D	FASTA	106	11,248
	10 20 30 40 50 60 MTKIIAVTAC PSGVAHTYMA AEALESAAKA KGWEVKVETQ GSIGLENELT AEDVASADMV 70 80 90 100 ILTKDIGIKF EERFAGKTIV RVNISDAVKR ADAIMSKIEA HLAQTA

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005674 Genomic DNA. Translation: AAN45457.1. AE014073 Genomic DNA. Translation: AAP18745.1.
RefSeq	NP_709750.1. NP_838934.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1026574. 1079932.
GenomeReviews	Gene locus SF4027 in contig AE005674_GR. Gene locus S3719 in contig AE014073_GR.
KEGG	sfl:SF4027. sfx:S3719.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P69819.
OMA	DIDEATC.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN45457.1-MON.
BRENDA	2.7.1.69. 189495.
Family and domain databases
InterPro	IPR013011. PTS_EIIB_2. IPR003353. PTS_IIB_fruc. [Graphical view]
Pfam	PF02379. PTS_IIB_fruc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00829. FRU. 1 hit.
PROSITE	PS51099. PTS_EIIB_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTFB2_SHIFL

Accession

Primary (citable) accession number: P69819
Secondary accession number(s): P32673

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 10, 2005
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)