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Protein

Fructose-like phosphotransferase enzyme IIB component 2

Gene

frwB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane.By similarity

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-fructose(Side 1) = [protein]-L-histidine + D-fructose 1-phosphate(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei10 – 101Phosphocysteine intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:FRWB-MONOMER.
ECOL316407:JW3922-MONOMER.

Protein family/group databases

TCDBi4.A.2.1.10. the pts fructose-mannitol (fru) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-like phosphotransferase enzyme IIB component 2 (EC:2.7.1.202)
Alternative name(s):
PTS system fructose-like EIIB component 2
Gene namesi
Name:frwB
Synonyms:yijK
Ordered Locus Names:b3950, JW3922
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11909. frwB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 106106Fructose-like phosphotransferase enzyme IIB component 2PRO_0000186501Add
BLAST

Proteomic databases

PaxDbiP69816.

Interactioni

Protein-protein interaction databases

BioGridi4262059. 13 interactions.
IntActiP69816. 4 interactions.
STRINGi511145.b3950.

Structurei

3D structure databases

ProteinModelPortaliP69816.
SMRiP69816. Positions 3-96.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 103103PTS EIIB type-2PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

Sequence similaritiesi

Contains 1 PTS EIIB type-2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108YRF. Bacteria.
COG1445. LUCA.
HOGENOMiHOG000231313.
KOiK11202.
OMAiGAMGMID.
PhylomeDBiP69816.

Family and domain databases

CDDicd05569. PTS_IIB_fructose. 1 hit.
InterProiIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003353. PTS_IIB_fruc.
[Graphical view]
PfamiPF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
TIGRFAMsiTIGR00829. FRU. 1 hit.
PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69816-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKIIAVTAC PSGVAHTYMA AEALESAAKA KGWEVKVETQ GSIGLENELT
60 70 80 90 100
AEDVASADMV ILTKDIGIKF EERFAGKTIV RVNISDAVKR ADAIMSKIEA

HLAQTA
Length:106
Mass (Da):11,248
Last modified:May 10, 2005 - v1
Checksum:iF712189F18489D3D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43056.1.
U00096 Genomic DNA. Translation: AAC76932.1.
AP009048 Genomic DNA. Translation: BAE77361.1.
PIRiA65202.
RefSeqiNP_418385.1. NC_000913.3.
WP_000161265.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76932; AAC76932; b3950.
BAE77361; BAE77361; BAE77361.
GeneIDi948447.
KEGGiecj:JW3922.
eco:b3950.
PATRICi32123421. VBIEscCol129921_4071.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43056.1.
U00096 Genomic DNA. Translation: AAC76932.1.
AP009048 Genomic DNA. Translation: BAE77361.1.
PIRiA65202.
RefSeqiNP_418385.1. NC_000913.3.
WP_000161265.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP69816.
SMRiP69816. Positions 3-96.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262059. 13 interactions.
IntActiP69816. 4 interactions.
STRINGi511145.b3950.

Protein family/group databases

TCDBi4.A.2.1.10. the pts fructose-mannitol (fru) family.

Proteomic databases

PaxDbiP69816.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76932; AAC76932; b3950.
BAE77361; BAE77361; BAE77361.
GeneIDi948447.
KEGGiecj:JW3922.
eco:b3950.
PATRICi32123421. VBIEscCol129921_4071.

Organism-specific databases

EchoBASEiEB1853.
EcoGeneiEG11909. frwB.

Phylogenomic databases

eggNOGiENOG4108YRF. Bacteria.
COG1445. LUCA.
HOGENOMiHOG000231313.
KOiK11202.
OMAiGAMGMID.
PhylomeDBiP69816.

Enzyme and pathway databases

BioCyciEcoCyc:FRWB-MONOMER.
ECOL316407:JW3922-MONOMER.

Miscellaneous databases

PROiP69816.

Family and domain databases

CDDicd05569. PTS_IIB_fructose. 1 hit.
InterProiIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003353. PTS_IIB_fruc.
[Graphical view]
PfamiPF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
TIGRFAMsiTIGR00829. FRU. 1 hit.
PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTFB2_ECOLI
AccessioniPrimary (citable) accession number: P69816
Secondary accession number(s): P32673, Q2M8P5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: September 7, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.