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Protein

PTS system fructose-like EIIB component 2

Gene

frwB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II FrwABC PTS system is involved in fructose transport.By similarity1 Publication

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-fructose(Side 1) = [protein]-L-histidine + D-fructose 1-phosphate(Side 2).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei10Phosphocysteine intermediateBy similarityCurated1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:FRWB-MONOMER
MetaCyc:FRWB-MONOMER

Protein family/group databases

TCDBi4.A.2.1.10 the pts fructose-mannitol (fru) family

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system fructose-like EIIB component 2By similarity (EC:2.7.1.202By similarity)
Alternative name(s):
Fructose-like phosphotransferase enzyme IIB component 2By similarity
Gene namesi
Name:frwB
Synonyms:yijK
Ordered Locus Names:b3950, JW3922
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11909 frwB

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001865011 – 106PTS system fructose-like EIIB component 2Add BLAST106

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10Phosphocysteine; by EIIAPROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP69816
PRIDEiP69816

Interactioni

Protein-protein interaction databases

BioGridi4262059, 13 interactors
IntActiP69816, 4 interactors
STRINGi316385.ECDH10B_4138

Structurei

3D structure databases

ProteinModelPortaliP69816
SMRiP69816
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 103PTS EIIB type-2PROSITE-ProRule annotationAdd BLAST103

Domaini

The PTS EIIB type-2 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108YRF Bacteria
COG1445 LUCA
HOGENOMiHOG000231313
KOiK11202
OMAiTYMAADV
PhylomeDBiP69816

Family and domain databases

CDDicd05569 PTS_IIB_fructose, 1 hit
InterProiView protein in InterPro
IPR036095 PTS_EIIB-like_sf
IPR013011 PTS_EIIB_2
IPR003501 PTS_EIIB_2/3
IPR003353 PTS_IIB_fruc
PfamiView protein in Pfam
PF02302 PTS_IIB, 1 hit
SUPFAMiSSF52794 SSF52794, 1 hit
TIGRFAMsiTIGR00829 FRU, 1 hit
PROSITEiView protein in PROSITE
PS51099 PTS_EIIB_TYPE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P69816-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKIIAVTAC PSGVAHTYMA AEALESAAKA KGWEVKVETQ GSIGLENELT
60 70 80 90 100
AEDVASADMV ILTKDIGIKF EERFAGKTIV RVNISDAVKR ADAIMSKIEA

HLAQTA
Length:106
Mass (Da):11,248
Last modified:May 10, 2005 - v1
Checksum:iF712189F18489D3D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA Translation: AAC43056.1
U00096 Genomic DNA Translation: AAC76932.1
AP009048 Genomic DNA Translation: BAE77361.1
PIRiA65202
RefSeqiNP_418385.1, NC_000913.3
WP_000161265.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76932; AAC76932; b3950
BAE77361; BAE77361; BAE77361
GeneIDi948447
KEGGiecj:JW3922
eco:b3950
PATRICifig|1411691.4.peg.2755

Similar proteinsi

Entry informationi

Entry nameiPTFB2_ECOLI
AccessioniPrimary (citable) accession number: P69816
Secondary accession number(s): P32673, Q2M8P5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: March 28, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health