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Protein

Multiphosphoryl transfer protein

Gene

fruB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in fructose transport.

Catalytic activityi

Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei62 – 621Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation
Active sitei299 – 2991Pros-phosphohistidine intermediate; for HPr activityPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • fructose import Source: EcoCyc
  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:FRUB-MONOMER.
ECOL316407:JW2156-MONOMER.

Protein family/group databases

TCDBi4.A.2.1.1. the pts fructose-mannitol (fru) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Multiphosphoryl transfer protein
Short name:
MTP
Alternative name(s):
Phosphotransferase FPr protein
Pseudo-HPr
Including the following 2 domains:
Phosphocarrier protein HPr
Short name:
Protein H
Fructose-specific phosphotransferase enzyme IIA component (EC:2.7.1.-)
Alternative name(s):
PTS system fructose-specific EIIA component
Gene namesi
Name:fruB
Synonyms:fpr, fruF
Ordered Locus Names:b2169, JW2156
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10005. fruB.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extrinsic component of plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Multiphosphoryl transfer proteinPRO_0000186516Add
BLAST

Proteomic databases

EPDiP69811.
PaxDbiP69811.
PRIDEiP69811.

2D gel databases

SWISS-2DPAGEP69811.

Expressioni

Inductioni

By fructose.

Interactioni

Protein-protein interaction databases

BioGridi4260464. 13 interactions.
DIPiDIP-48096N.
IntActiP69811. 7 interactions.
MINTiMINT-1263101.
STRINGi511145.b2169.

Structurei

3D structure databases

ProteinModelPortaliP69811.
SMRiP69811. Positions 1-143, 289-364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 142141PTS EIIA type-2PROSITE-ProRule annotationAdd
BLAST
Domaini285 – 37591HPrPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni156 – 284129M domainAdd
BLAST

Domaini

In contrast to classical PTS systems, the fructose PTS has no requirement for HPr; FPr combines a IIA domain with a HPr domain.
The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.

Sequence similaritiesi

Contains 1 HPr domain.PROSITE-ProRule annotation
Contains 1 PTS EIIA type-2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105DTZ. Bacteria.
COG1925. LUCA.
COG4668. LUCA.
HOGENOMiHOG000278131.
KOiK02768.
K11183.
OMAiQGIEWGE.
OrthoDBiEOG6XDGX2.

Family and domain databases

Gene3Di3.30.1340.10. 1 hit.
3.40.930.10. 2 hits.
InterProiIPR000032. HPr-like.
IPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
IPR001020. PTS_HPr_His_P_site.
IPR002114. PTS_HPr_Ser_P_site.
[Graphical view]
PfamiPF00381. PTS-HPr. 1 hit.
PF00359. PTS_EIIA_2. 1 hit.
[Graphical view]
PRINTSiPR00107. PHOSPHOCPHPR.
SUPFAMiSSF55594. SSF55594. 1 hit.
SSF55804. SSF55804. 2 hits.
TIGRFAMsiTIGR01003. PTS_HPr_family. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
PS51350. PTS_HPR_DOM. 1 hit.
PS00369. PTS_HPR_HIS. 1 hit.
PS00589. PTS_HPR_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFQLSVQDIH PGEKAGDKEE AIRQVAAALV QAGNVAEGYV NGMLAREQQT
60 70 80 90 100
STFLGNGIAI PHGTTDTRDQ VLKTGVQVFQ FPEGVTWGDG QVAYVAIGIA
110 120 130 140 150
ASSDEHLGLL RQLTHVLSDD SVAEQLKSAT TAEELRALLM GEKQSEQLKL
160 170 180 190 200
DNEMLTLDIV ASDLLTLQAL NAARLKEAGA VDATFVTKAI NEQPLNLGQG
210 220 230 240 250
IWLSDSAEGN LRSAIAVSRA ANAFDVDGET AAMLVSVAMN DDQPIAVLKR
260 270 280 290 300
LADLLLDNKA DRLLKADAAT LLALLTSDDA PTDDVLSAEF VVRNEHGLHA
310 320 330 340 350
RPGTMLVNTI KQFNSDITVT NLDGTGKPAN GRSLMKVVAL GVKKGHRLRF
360 370
TAQGADAEQA LKAIGDAIAA GLGEGA
Length:376
Mass (Da):39,648
Last modified:May 10, 2005 - v1
Checksum:iD4D953E69E2090FF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti59 – 591A → T (PubMed:9097040).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S72443 Genomic DNA. Translation: AAB31084.1.
U00007 Genomic DNA. Translation: AAA60534.1.
U00096 Genomic DNA. Translation: AAC75230.1.
AP009048 Genomic DNA. Translation: BAA15978.2.
X53948 Genomic DNA. Translation: CAA37895.1.
PIRiI53564.
RefSeqiNP_416674.1. NC_000913.3.
WP_000487246.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75230; AAC75230; b2169.
BAA15978; BAA15978; BAA15978.
GeneIDi946677.
KEGGiecj:JW2156.
eco:b2169.
PATRICi32119687. VBIEscCol129921_2254.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S72443 Genomic DNA. Translation: AAB31084.1.
U00007 Genomic DNA. Translation: AAA60534.1.
U00096 Genomic DNA. Translation: AAC75230.1.
AP009048 Genomic DNA. Translation: BAA15978.2.
X53948 Genomic DNA. Translation: CAA37895.1.
PIRiI53564.
RefSeqiNP_416674.1. NC_000913.3.
WP_000487246.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP69811.
SMRiP69811. Positions 1-143, 289-364.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260464. 13 interactions.
DIPiDIP-48096N.
IntActiP69811. 7 interactions.
MINTiMINT-1263101.
STRINGi511145.b2169.

Protein family/group databases

TCDBi4.A.2.1.1. the pts fructose-mannitol (fru) family.

2D gel databases

SWISS-2DPAGEP69811.

Proteomic databases

EPDiP69811.
PaxDbiP69811.
PRIDEiP69811.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75230; AAC75230; b2169.
BAA15978; BAA15978; BAA15978.
GeneIDi946677.
KEGGiecj:JW2156.
eco:b2169.
PATRICi32119687. VBIEscCol129921_2254.

Organism-specific databases

EchoBASEiEB0005.
EcoGeneiEG10005. fruB.

Phylogenomic databases

eggNOGiENOG4105DTZ. Bacteria.
COG1925. LUCA.
COG4668. LUCA.
HOGENOMiHOG000278131.
KOiK02768.
K11183.
OMAiQGIEWGE.
OrthoDBiEOG6XDGX2.

Enzyme and pathway databases

BioCyciEcoCyc:FRUB-MONOMER.
ECOL316407:JW2156-MONOMER.

Miscellaneous databases

PROiP69811.

Family and domain databases

Gene3Di3.30.1340.10. 1 hit.
3.40.930.10. 2 hits.
InterProiIPR000032. HPr-like.
IPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
IPR001020. PTS_HPr_His_P_site.
IPR002114. PTS_HPr_Ser_P_site.
[Graphical view]
PfamiPF00381. PTS-HPr. 1 hit.
PF00359. PTS_EIIA_2. 1 hit.
[Graphical view]
PRINTSiPR00107. PHOSPHOCPHPR.
SUPFAMiSSF55594. SSF55594. 1 hit.
SSF55804. SSF55804. 2 hits.
TIGRFAMsiTIGR01003. PTS_HPr_family. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
PS51350. PTS_HPR_DOM. 1 hit.
PS00369. PTS_HPR_HIS. 1 hit.
PS00589. PTS_HPR_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Automated multiplex sequencing of the E.coli genome."
    Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M.
    Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / BHB2600.
  2. "Sequence of the fruB gene of Escherichia coli encoding the diphosphoryl transfer protein (DTP) of the phosphoenolpyruvate: sugar phosphotransferase system."
    Reizer J., Reizer A., Kornberg H.L., Saier M.H. Jr.
    FEMS Microbiol. Lett. 118:159-162(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 59.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Sequence similarities between the gene specifying 1-phosphofructokinase (fruK), genes specifying other kinases in Escherichia coli K12, and lacC of Staphylococcus aureus."
    Orchard L.M.D., Kornberg H.L.
    Proc. R. Soc. B 242:87-90(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 274-376.
    Strain: K12.

Entry informationi

Entry nameiPTFAH_ECOLI
AccessioniPrimary (citable) accession number: P69811
Secondary accession number(s): P24217, P94759
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: July 6, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.