Fructose-like phosphotransferase enzyme IIB component 1

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P69808 (PTFB1_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 52. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Fructose-like phosphotransferase enzyme IIB component 1
EC=2.7.1.69

Alternative name(s):
PTS system fructose-like EIIB component 1

Gene names

Name:	fryB
Synonyms:	ypdH
Ordered Locus Names:	b2387, JW5389

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	108 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cytoplasm Potential.
Domain	The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-2 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 108

108

Fructose-like phosphotransferase enzyme IIB component 1

PRO_0000186700

Regions

Domain

1 – 104

104

PTS EIIB type-2

Sites

Active site

Phosphocysteine intermediate By similarity

Secondary structure

108

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P69808 [UniParc].

Last modified May 10, 2005. Version 1.
Checksum: EADDEFB237FB72A9
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FASTA

108

11,735

        10         20         30         40         50         60 
MSKKLIALCA CPMGLAHTFM AAQALEEAAV EAGYEVKIET QGADGIQNRL TAQDIAEATI 

        70         80         90        100 
IIHSVAVTPE DNERFESRDV YEITLQDAIK NAAGIIKEIE EMIASEQQ

« Hide

References

[1]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U00096 Genomic DNA. Translation: AAC75446.1.
AP009048 Genomic DNA. Translation: BAA16257.2.

PIR

H65012.

RefSeq

NP_416888.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KYR	NMR	-	A	1-108	[»]

ProteinModelPortal

P69808.

SMR

P69808. Positions 1-108.

ModBase

Search...

Protein family/group databases

TCDB

4.A.2.1.11. PTS fructose-mannitol (Fru) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000004424; EBESCP00000004424; EBESCG00000003611.
EBESCT00000016643; EBESCP00000015934; EBESCG00000015702.

GeneID

949087.

GenomeReviews

Gene locus JW5389 in contig AP009048_GR.
Gene locus b2387 in contig U00096_GR.

KEGG

ecj:JW5389.
eco:b2387.

PATRIC

32120153. VBIEscCol129921_2485.

Organism-specific databases

EchoBASE

EB3907.

EcoGene

EG14155. fryB.

Phylogenomic databases

eggNOG

COG1445.

GeneTree

EBGT00070000031719.

HOGENOM

HBG564028.

OMA

AVCACPM.

PhylomeDB

P69808.

ProtClustDB

CLSK880379.

Enzyme and pathway databases

BioCyc

EcoCyc:G7250-MONOMER.

Gene expression databases

Genevestigator

P69808.

Family and domain databases

InterPro

IPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003353. PTS_IIB_fruc.
[Graphical view]

K11202.

Pfam

PF02302. PTS_IIB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00829. FRU. 1 hit.

PROSITE

PS51099. PTS_EIIB_TYPE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PTFB1_ECOLI

Accession

Primary (citable) accession number: P69808
Secondary accession number(s): P76525, P78264

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 10, 2005
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents