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Protein

PTS system fructose-like EIIB component 1

Gene

fryB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II FryABC PTS system is involved in fructose transport.By similarity

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-fructose(Side 1) = [protein]-L-histidine + D-fructose 1-phosphate(Side 2).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei11Phosphocysteine intermediateBy similarityCurated1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G7250-MONOMER.

Protein family/group databases

TCDBi4.A.2.1.11. the pts fructose-mannitol (fru) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system fructose-like EIIB component 1By similarity (EC:2.7.1.202By similarity)
Alternative name(s):
Fructose-like phosphotransferase enzyme IIB component 1By similarity
Gene namesi
Name:fryB
Synonyms:ypdH
Ordered Locus Names:b2387, JW5389
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14155. fryB.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001867001 – 108PTS system fructose-like EIIB component 1Add BLAST108

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei11Phosphocysteine; by EIIAPROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP69808.
PRIDEiP69808.

Interactioni

Protein-protein interaction databases

BioGridi4260782. 10 interactors.
STRINGi316385.ECDH10B_2552.

Structurei

Secondary structure

1108
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 13Combined sources10
Helixi15 – 31Combined sources17
Beta strandi33 – 42Combined sources10
Beta strandi45 – 48Combined sources4
Helixi52 – 57Combined sources6
Beta strandi59 – 67Combined sources9
Helixi72 – 75Combined sources4
Beta strandi80 – 84Combined sources5
Helixi87 – 90Combined sources4
Helixi92 – 107Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KYRNMR-A1-108[»]
ProteinModelPortaliP69808.
SMRiP69808.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69808.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 104PTS EIIB type-2PROSITE-ProRule annotationAdd BLAST104

Domaini

The PTS EIIB type-2 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105R6B. Bacteria.
COG1445. LUCA.
HOGENOMiHOG000231313.
KOiK11202.
PhylomeDBiP69808.

Family and domain databases

CDDicd05569. PTS_IIB_fructose. 1 hit.
InterProiView protein in InterPro
IPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003353. PTS_IIB_fruc.
PfamiView protein in Pfam
PF02302. PTS_IIB. 1 hit.
SUPFAMiSSF52794. SSF52794. 1 hit.
TIGRFAMsiTIGR00829. FRU. 1 hit.
PROSITEiView protein in PROSITE
PS51099. PTS_EIIB_TYPE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P69808-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKKLIALCA CPMGLAHTFM AAQALEEAAV EAGYEVKIET QGADGIQNRL
60 70 80 90 100
TAQDIAEATI IIHSVAVTPE DNERFESRDV YEITLQDAIK NAAGIIKEIE

EMIASEQQ
Length:108
Mass (Da):11,735
Last modified:May 10, 2005 - v1
Checksum:iEADDEFB237FB72A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75446.1.
AP009048 Genomic DNA. Translation: BAA16257.2.
PIRiH65012.
RefSeqiNP_416888.1. NC_000913.3.
WP_000038456.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75446; AAC75446; b2387.
BAA16257; BAA16257; BAA16257.
GeneIDi949087.
KEGGiecj:JW5389.
eco:b2387.
PATRICifig|1411691.4.peg.4341.

Similar proteinsi

Entry informationi

Entry nameiPTFB1_ECOLI
AccessioniPrimary (citable) accession number: P69808
Secondary accession number(s): P76525, P78264
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: July 5, 2017
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references