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Protein

Fructose-like phosphotransferase enzyme IIB component 1

Gene

fryB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane.By similarity

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-fructose(Side 1) = [protein]-L-histidine + D-fructose 1-phosphate(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei11 – 111Phosphocysteine intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G7250-MONOMER.
ECOL316407:JW5389-MONOMER.

Protein family/group databases

TCDBi4.A.2.1.11. the pts fructose-mannitol (fru) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-like phosphotransferase enzyme IIB component 1 (EC:2.7.1.202)
Alternative name(s):
PTS system fructose-like EIIB component 1
Gene namesi
Name:fryB
Synonyms:ypdH
Ordered Locus Names:b2387, JW5389
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14155. fryB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 108108Fructose-like phosphotransferase enzyme IIB component 1PRO_0000186700Add
BLAST

Proteomic databases

PaxDbiP69808.

Interactioni

Protein-protein interaction databases

BioGridi4260782. 10 interactions.
STRINGi511145.b2387.

Structurei

Secondary structure

1
108
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 1310Combined sources
Helixi15 – 3117Combined sources
Beta strandi33 – 4210Combined sources
Beta strandi45 – 484Combined sources
Helixi52 – 576Combined sources
Beta strandi59 – 679Combined sources
Helixi72 – 754Combined sources
Beta strandi80 – 845Combined sources
Helixi87 – 904Combined sources
Helixi92 – 10716Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KYRNMR-A1-108[»]
ProteinModelPortaliP69808.
SMRiP69808. Positions 1-108.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69808.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 104104PTS EIIB type-2PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

Sequence similaritiesi

Contains 1 PTS EIIB type-2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105R6B. Bacteria.
COG1445. LUCA.
HOGENOMiHOG000231313.
KOiK11202.
OMAiDNERFAT.
PhylomeDBiP69808.

Family and domain databases

CDDicd05569. PTS_IIB_fructose. 1 hit.
InterProiIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003353. PTS_IIB_fruc.
[Graphical view]
PfamiPF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
TIGRFAMsiTIGR00829. FRU. 1 hit.
PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69808-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKKLIALCA CPMGLAHTFM AAQALEEAAV EAGYEVKIET QGADGIQNRL
60 70 80 90 100
TAQDIAEATI IIHSVAVTPE DNERFESRDV YEITLQDAIK NAAGIIKEIE

EMIASEQQ
Length:108
Mass (Da):11,735
Last modified:May 10, 2005 - v1
Checksum:iEADDEFB237FB72A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75446.1.
AP009048 Genomic DNA. Translation: BAA16257.2.
PIRiH65012.
RefSeqiNP_416888.1. NC_000913.3.
WP_000038456.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75446; AAC75446; b2387.
BAA16257; BAA16257; BAA16257.
GeneIDi949087.
KEGGiecj:JW5389.
eco:b2387.
PATRICi32120153. VBIEscCol129921_2485.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75446.1.
AP009048 Genomic DNA. Translation: BAA16257.2.
PIRiH65012.
RefSeqiNP_416888.1. NC_000913.3.
WP_000038456.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KYRNMR-A1-108[»]
ProteinModelPortaliP69808.
SMRiP69808. Positions 1-108.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260782. 10 interactions.
STRINGi511145.b2387.

Protein family/group databases

TCDBi4.A.2.1.11. the pts fructose-mannitol (fru) family.

Proteomic databases

PaxDbiP69808.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75446; AAC75446; b2387.
BAA16257; BAA16257; BAA16257.
GeneIDi949087.
KEGGiecj:JW5389.
eco:b2387.
PATRICi32120153. VBIEscCol129921_2485.

Organism-specific databases

EchoBASEiEB3907.
EcoGeneiEG14155. fryB.

Phylogenomic databases

eggNOGiENOG4105R6B. Bacteria.
COG1445. LUCA.
HOGENOMiHOG000231313.
KOiK11202.
OMAiDNERFAT.
PhylomeDBiP69808.

Enzyme and pathway databases

BioCyciEcoCyc:G7250-MONOMER.
ECOL316407:JW5389-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP69808.
PROiP69808.

Family and domain databases

CDDicd05569. PTS_IIB_fructose. 1 hit.
InterProiIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003353. PTS_IIB_fruc.
[Graphical view]
PfamiPF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
TIGRFAMsiTIGR00829. FRU. 1 hit.
PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTFB1_ECOLI
AccessioniPrimary (citable) accession number: P69808
Secondary accession number(s): P76525, P78264
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: September 7, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.