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Protein

PTS system mannose-specific EIID component

Gene

manZ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane (PubMed:2951378, PubMed:2999119). The enzyme II ManXYZ PTS system is involved in mannose transport (PubMed:2951378, PubMed:2999119). Also functions as a receptor for bacterial chemotaxis and is required for infection of the cell by bacteriophage lambda where it most likely functions as a pore for penetration of lambda DNA (PubMed:4604906, PubMed:353494).4 Publications

GO - Molecular functioni

GO - Biological processi

  • glucose import into cell Source: EcoCyc
  • mannose transport Source: EcoCyc
  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:MANZ-MONOMER.
ECOL316407:JW1808-MONOMER.
MetaCyc:MANZ-MONOMER.

Protein family/group databases

TCDBi4.A.6.1.1. the pts mannose-fructose-sorbose (man) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system mannose-specific EIID component1 Publication
Alternative name(s):
EII-M-Man1 Publication
EIID-Man1 Publication
Mannose permease IID component1 Publication
Gene namesi
Name:manZ
Synonyms:gptB, ptsM
Ordered Locus Names:b1819, JW1808
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10569. manZ.

Subcellular locationi

  • Cell inner membrane 1 Publication; Single-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 258258Cytoplasmic1 PublicationAdd
BLAST
Transmembranei259 – 27820Helical1 PublicationAdd
BLAST
Topological domaini279 – 2835Periplasmic1 Publication

GO - Cellular componenti

  • integral component of plasma membrane Source: CACAO
  • membrane Source: UniProtKB
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 283283PTS system mannose-specific EIID componentPRO_0000186650Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-formylmethionine1 Publication

Keywords - PTMi

Formylation

Proteomic databases

EPDiP69805.
PaxDbiP69805.

Interactioni

Protein-protein interaction databases

BioGridi4259146. 324 interactions.
IntActiP69805. 1 interaction.
STRINGi511145.b1819.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 281271PTS EIIDPROSITE-ProRule annotationAdd
BLAST

Domaini

The EIID domain, with its homologous EIIC domain, forms the PTS system translocation channel and contains part of its specific substrate-binding site.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 PTS EIID domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105F3R. Bacteria.
COG3716. LUCA.
HOGENOMiHOG000098670.
InParanoidiP69805.
KOiK02796.

Family and domain databases

InterProiIPR004704. PTS_IID_comp.
IPR018405. PTS_IID_comp_subgr.
[Graphical view]
PfamiPF03613. EIID-AGA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00828. EIID-AGA. 1 hit.
PROSITEiPS51108. PTS_EIID. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69805-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDTTQTTTE KKLTQSDIRG VFLRSNLFQG SWNFERMQAL GFCFSMVPAI
60 70 80 90 100
RRLYPENNEA RKQAIRRHLE FFNTQPFVAA PILGVTLALE EQRANGAEID
110 120 130 140 150
DGAINGIKVG LMGPLAGVGD PIFWGTVRPV FAALGAGIAM SGSLLGPLLF
160 170 180 190 200
FILFNLVRLA TRYYGVAYGY SKGIDIVKDM GGGFLQKLTE GASILGLFVM
210 220 230 240 250
GALVNKWTHV NIPLVVSRIT DQTGKEHVTT VQTILDQLMP GLVPLLLTFA
260 270 280
CMWLLRKKVN PLWIIVGFFV IGIAGYACGL LGL
Length:283
Mass (Da):30,955
Last modified:November 16, 2011 - v2
Checksum:iE29ED1D7040AE67D
GO

Sequence cautioni

The sequence AAA24445 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAA15631 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02699 Genomic DNA. Translation: AAA24445.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74889.2.
AP009048 Genomic DNA. Translation: BAA15631.1. Different initiation.
PIRiA30288. WQECMM.
RefSeqiNP_416333.4. NC_000913.3.
WP_000228655.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74889; AAC74889; b1819.
BAA15631; BAA15631; BAA15631.
GeneIDi946342.
KEGGiecj:JW1808.
eco:b1819.
PATRICi32118957. VBIEscCol129921_1896.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02699 Genomic DNA. Translation: AAA24445.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74889.2.
AP009048 Genomic DNA. Translation: BAA15631.1. Different initiation.
PIRiA30288. WQECMM.
RefSeqiNP_416333.4. NC_000913.3.
WP_000228655.1. NZ_LN832404.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259146. 324 interactions.
IntActiP69805. 1 interaction.
STRINGi511145.b1819.

Protein family/group databases

TCDBi4.A.6.1.1. the pts mannose-fructose-sorbose (man) family.

Proteomic databases

EPDiP69805.
PaxDbiP69805.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74889; AAC74889; b1819.
BAA15631; BAA15631; BAA15631.
GeneIDi946342.
KEGGiecj:JW1808.
eco:b1819.
PATRICi32118957. VBIEscCol129921_1896.

Organism-specific databases

EchoBASEiEB0564.
EcoGeneiEG10569. manZ.

Phylogenomic databases

eggNOGiENOG4105F3R. Bacteria.
COG3716. LUCA.
HOGENOMiHOG000098670.
InParanoidiP69805.
KOiK02796.

Enzyme and pathway databases

BioCyciEcoCyc:MANZ-MONOMER.
ECOL316407:JW1808-MONOMER.
MetaCyc:MANZ-MONOMER.

Miscellaneous databases

PROiP69805.

Family and domain databases

InterProiIPR004704. PTS_IID_comp.
IPR018405. PTS_IID_comp_subgr.
[Graphical view]
PfamiPF03613. EIID-AGA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00828. EIID-AGA. 1 hit.
PROSITEiPS51108. PTS_EIID. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTND_ECOLI
AccessioniPrimary (citable) accession number: P69805
Secondary accession number(s): P08188
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 16, 2011
Last modified: September 7, 2016
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.