P69805 (PTND_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannose permease IID component

Alternative name(s):
EII-M-Man
EIID-Man
PTS system mannose-specific EIID component

Gene names

Name:	manZ
Synonyms:	gptB, ptsM
Ordered Locus Names:	b1819, JW1808

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in mannose transport.
Subcellular location	Cell inner membrane; Single-pass membrane protein.
Domain	The EIID domain, with its homologous EIIC domain, forms the PTS system translocation channel and contains part of its specific substrate-binding site.
Sequence similarities	Contains 1 PTS EIID domain.
Sequence caution	The sequence AAA24445.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAA15631.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from direct assay PubMed 2999119. Source: EcoCyc
Cellular_component	integral to plasma membrane Inferred from direct assay PubMed 2999119. Source: EcoCyc
Molecular_function	protein-N(PI)-phosphohistidine-mannose phosphotransferase system transporter activity Inferred from direct assay PubMed 2999119. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Mannose permease IID component		PRO_0000186650
Regions
	Topological domain	1 – 258	258	Cytoplasmic Probable
	Transmembrane	259 – 278	20	Helical; Probable
	Topological domain	279 – 283	5	Periplasmic Probable
	Domain	11 – 281	271	PTS EIID

Sequences

	Sequence		Length	Mass (Da)
	P69805 [UniParc]. Last modified November 16, 2011. Version 2. Checksum: E29ED1D7040AE67D	FASTA	283	30,955
	10 20 30 40 50 60 MVDTTQTTTE KKLTQSDIRG VFLRSNLFQG SWNFERMQAL GFCFSMVPAI RRLYPENNEA 70 80 90 100 110 120 RKQAIRRHLE FFNTQPFVAA PILGVTLALE EQRANGAEID DGAINGIKVG LMGPLAGVGD 130 140 150 160 170 180 PIFWGTVRPV FAALGAGIAM SGSLLGPLLF FILFNLVRLA TRYYGVAYGY SKGIDIVKDM 190 200 210 220 230 240 GGGFLQKLTE GASILGLFVM GALVNKWTHV NIPLVVSRIT DQTGKEHVTT VQTILDQLMP 250 260 270 280 GLVPLLLTFA CMWLLRKKVN PLWIIVGFFV IGIAGYACGL LGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mannose permease of Escherichia coli consists of three different proteins. Amino acid sequence and function in sugar transport, sugar phosphorylation, and penetration of phage lambda DNA." Erni B., Zanolari B., Kocher H.P. J. Biol. Chem. 262:5238-5247(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Topology of the Escherichia coli mannose transporter." Huber F., Erni B. Protein Sci. 4 Suppl. 1:111-111(1995) Cited for: TOPOLOGY.
[6]	"Membrane topology of the mannose transporter of Escherichia coli K12." Huber F., Erni B. Eur. J. Biochem. 239:810-817(1996) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY.
[7]	"The mannose transporter of Escherichia coli K12: oligomeric structure, and function of two conserved cysteines." Rhiel E., Flukiger K., Wehrli C., Erni B. Biol. Chem. Hoppe-Seyler 375:551-559(1994) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY.
[8]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02699 Genomic DNA. Translation: AAA24445.1. Different initiation. U00096 Genomic DNA. Translation: AAC74889.2. AP009048 Genomic DNA. Translation: BAA15631.1. Different initiation.
PIR	WQECMM. A30288.
RefSeq	NP_416333.4. NC_000913.2. YP_490080.1. NC_007779.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P69805. 1 interaction.
STRING	511145.b1819.
Protein family/group databases
TCDB	4.A.6.1.1. PTS mannose-fructose-sorbose (Man) family.
Proteomic databases
PaxDb	P69805.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74889; AAC74889; b1819. BAA15631; BAA15631; BAA15631.
GeneID	12930152. 946342.
KEGG	ecj:Y75_p1794. eco:b1819.
PATRIC	32118957. VBIEscCol129921_1896.
Organism-specific databases
EchoBASE	EB0564.
EcoGene	EG10569. manZ.
Phylogenomic databases
eggNOG	COG3716.
HOGENOM	HOG000098670.
KO	K02796.
ProtClustDB	PRK11103.
Enzyme and pathway databases
BioCyc	EcoCyc:MANZ-MONOMER. ECOL316407:JW1808-MONOMER. MetaCyc:MANZ-MONOMER.
Gene expression databases
Genevestigator	P69805.
Family and domain databases
InterPro	IPR004704. PTS_IID_comp. IPR018405. PTS_IID_comp_subgr. [Graphical view]
Pfam	PF03613. EIID-AGA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00828. EIID-AGA. 1 hit.
PROSITE	PS51108. PTS_EIID. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTND_ECOLI

Accession

Primary (citable) accession number: P69805
Secondary accession number(s): P08188

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	November 16, 2011
Last modified:	May 1, 2013
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program