ID PTNAB_SHIFL Reviewed; 323 AA. AC P69800; P08186; Q47350; DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot. DT 23-JAN-2007, sequence version 2. DT 16-JUN-2009, entry version 38. DE RecName: Full=PTS system mannose-specific EIIAB component; DE AltName: Full=EIIAB-Man; DE Includes: DE RecName: Full=Mannose-specific phosphotransferase enzyme IIA component; DE EC=2.7.1.-; DE AltName: Full=PTS system mannose-specific EIIA component; DE AltName: Full=EIII-Man; DE Includes: DE RecName: Full=Mannose-specific phosphotransferase enzyme IIB component; DE EC=2.7.1.69; DE AltName: Full=PTS system mannose-specific EIIB component; GN Name=manX; OrderedLocusNames=SF1411, S1526; OS Shigella flexneri. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Shigella. OX NCBI_TaxID=623; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=301 / Serotype 2a; RX MEDLINE=22272406; PubMed=12384590; DOI=10.1093/nar/gkf566; RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., RA Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., RA Sun L., Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., RA Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., RA Yu J.; RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity RT through comparison with genomes of Escherichia coli K12 and O157."; RL Nucleic Acids Res. 30:4432-4441(2002). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 700930 / 2457T / Serotype 2a; RX MEDLINE=22590274; PubMed=12704152; RX DOI=10.1128/IAI.71.5.2775-2786.2003; RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., RA Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., RA Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., RA Schwartz D.C., Blattner F.R.; RT "Complete genome sequence and comparative genomics of Shigella RT flexneri serotype 2a strain 2457T."; RL Infect. Immun. 71:2775-2786(2003). CC -!- FUNCTION: The phosphoenolpyruvate-dependent sugar CC phosphotransferase system (sugar PTS), a major carbohydrate active CC -transport system, catalyzes the phosphorylation of incoming sugar CC substrates concomitantly with their translocation across the cell CC membrane. This system is involved in mannose transport (By CC similarity). CC -!- CATALYTIC ACTIVITY: Protein EIIA N(pi)-phospho-L-histidine + CC protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L- CC histidine/cysteine. CC -!- CATALYTIC ACTIVITY: Protein EIIB N(pi)-phospho-L- CC histidine/cysteine + sugar = protein EIIB + sugar phosphate. CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). CC -!- DOMAIN: The EIIA domain is phosphorylated by phospho-HPr on a CC histidyl residue. Then, it transfers the phosphoryl group to the CC EIIB domain. CC -!- DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a CC cysteinyl or histidyl residue, depending on the transported sugar. CC Then, it transfers the phosphoryl group to the sugar substrate CC concomitantly with the sugar uptake processed by the EIIC domain. CC -!- SIMILARITY: Contains 1 PTS EIIA type-4 domain. CC -!- SIMILARITY: Contains 1 PTS EIIB type-4 domain. CC ----------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution-NoDerivs License CC ----------------------------------------------------------------------- DR EMBL; AE005674; AAN43012.1; -; Genomic_DNA. DR EMBL; AE014073; AAP16907.1; -; Genomic_DNA. DR RefSeq; NP_707305.1; -. DR RefSeq; NP_837100.1; -. DR SMR; P69800; 2-130. DR GeneID; 1024605; -. DR GeneID; 1077900; -. DR GenomeReviews; AE005674_GR; SF1411. DR GenomeReviews; AE014073_GR; S1526. DR KEGG; sfl:SF1411; -. DR KEGG; sfx:S1526; -. DR HOGENOM; P69800; -. DR OMA; P69800; PSEGPDD. DR BioCyc; SFLE198214:AAN43012.1-MON; -. DR BRENDA; 2.7.1.69; 189495. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0016021; C:integral to membrane; IEA:InterPro. DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW. DR GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosph...; IEA:EC. DR GO; GO:0005351; F:sugar:hydrogen symporter activity; IEA:UniProtKB-KW. DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phospho...; IEA:UniProtKB-KW. DR InterPro; IPR004701; PTS_EIIA_fruc. DR InterPro; IPR013789; PTS_EIIA_fruc_sub. DR InterPro; IPR004720; PTS_IIB_sorbose-sp. DR InterPro; IPR018455; PTS_IIB_sorbose-sp_subgr. DR Gene3D; G3DSA:3.40.35.10; PTS_IIB_sorb; 1. DR Pfam; PF03610; EIIA-man; 1. DR Pfam; PF03830; PTSIIB_sorb; 1. DR ProDom; PD008332; PTSIIB_sorb; 1. DR TIGRFAMs; TIGR00824; EIIA-man; 1. DR TIGRFAMs; TIGR00854; pts-sorbose; 1. DR PROSITE; PS51096; PTS_EIIA_TYPE_4; 1. DR PROSITE; PS51101; PTS_EIIB_TYPE_4; 1. PE 3: Inferred from homology; KW Acetylation; Complete proteome; Cytoplasm; Kinase; KW Phosphotransferase system; Sugar transport; Transferase; Transport. FT INIT_MET 1 1 Removed (By similarity). FT CHAIN 2 323 PTS system mannose-specific EIIAB FT component. FT /FTId=PRO_0000186656. FT DOMAIN 2 126 PTS EIIA type-4. FT DOMAIN 160 323 PTS EIIB type-4. FT REGION 137 155 Hinge. FT ACT_SITE 10 10 Tele-phosphohistidine intermediate; for FT EIIA activity (By similarity). FT ACT_SITE 175 175 Pros-phosphohistidine intermediate; for FT EIIB activity (By similarity). FT MOD_RES 55 55 N6-acetyllysine (By similarity). FT MOD_RES 234 234 N6-acetyllysine (By similarity). SQ SEQUENCE 323 AA; 35048 MW; A446B79421B8C040 CRC64; MTIAIVIGTH GWAAEQLLKT AEMLLGEQEN VGWIDFVPGE NAETLIEKYN AQLAKLDTTK GVLFLVDTWG GSPFNAASRI VVDKEHYEVI AGVNIPMLVE TLMARDDDPS FDELVALAVE TGREGVKALK AKPVEKAAPA PAAAAPKAAP TPAKPMGPND YMVIGLARID DRLIHGQVAT RWTKETNVSR IIVVSDEVAA DTVRKTLLTQ VAPPGVTAHV VDVAKMIRVY NNPKYAGERV MLLFTNPTDV ERLVEGGVKI TSVNVGGMAF RQGKTQVNNA VSVDEKDIEA FKKLNARGIE LEVRKVSTDP KLKMMDLISK IDK //