ID   PTNAB_ECOL6             Reviewed;         323 AA.
AC   P69798; P08186; Q47350;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   16-JUN-2009, entry version 35.
DE   RecName: Full=PTS system mannose-specific EIIAB component;
DE   AltName: Full=EIIAB-Man;
DE   Includes:
DE     RecName: Full=Mannose-specific phosphotransferase enzyme IIA component;
DE              EC=2.7.1.-;
DE     AltName: Full=PTS system mannose-specific EIIA component;
DE     AltName: Full=EIII-Man;
DE   Includes:
DE     RecName: Full=Mannose-specific phosphotransferase enzyme IIB component;
DE              EC=2.7.1.69;
DE     AltName: Full=PTS system mannose-specific EIIB component;
GN   Name=manX; OrderedLocusNames=c2223;
OS   Escherichia coli O6.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=217992;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O6:H1 / CFT073 / ATCC 700928 / UPEC;
RX   MEDLINE=22388234; PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P.,
RA   Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D.,
RA   Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T.,
RA   Mobley H.L.T., Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence
RT   of uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: The phosphoenolpyruvate-dependent sugar
CC       phosphotransferase system (sugar PTS), a major carbohydrate active
CC       -transport system, catalyzes the phosphorylation of incoming sugar
CC       substrates concomitantly with their translocation across the cell
CC       membrane. This system is involved in mannose transport (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: Protein EIIA N(pi)-phospho-L-histidine +
CC       protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-
CC       histidine/cysteine.
CC   -!- CATALYTIC ACTIVITY: Protein EIIB N(pi)-phospho-L-
CC       histidine/cysteine + sugar = protein EIIB + sugar phosphate.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- DOMAIN: The EIIA domain is phosphorylated by phospho-HPr on a
CC       histidyl residue. Then, it transfers the phosphoryl group to the
CC       EIIB domain.
CC   -!- DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a
CC       cysteinyl or histidyl residue, depending on the transported sugar.
CC       Then, it transfers the phosphoryl group to the sugar substrate
CC       concomitantly with the sugar uptake processed by the EIIC domain.
CC   -!- SIMILARITY: Contains 1 PTS EIIA type-4 domain.
CC   -!- SIMILARITY: Contains 1 PTS EIIB type-4 domain.
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DR   EMBL; AE014075; AAN80682.1; -; Genomic_DNA.
DR   RefSeq; NP_754117.1; -.
DR   SMR; P69798; 2-130.
DR   GeneID; 1036702; -.
DR   GenomeReviews; AE014075_GR; c2223.
DR   KEGG; ecc:c2223; -.
DR   HOGENOM; P69798; -.
DR   OMA; P69798; PSEGPDD.
DR   BRENDA; 2.7.1.69; 292881.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:InterPro.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosph...; IEA:EC.
DR   GO; GO:0005351; F:sugar:hydrogen symporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phospho...; IEA:UniProtKB-KW.
DR   InterPro; IPR004701; PTS_EIIA_fruc.
DR   InterPro; IPR013789; PTS_EIIA_fruc_sub.
DR   InterPro; IPR004720; PTS_IIB_sorbose-sp.
DR   InterPro; IPR018455; PTS_IIB_sorbose-sp_subgr.
DR   Gene3D; G3DSA:3.40.35.10; PTS_IIB_sorb; 1.
DR   Pfam; PF03610; EIIA-man; 1.
DR   Pfam; PF03830; PTSIIB_sorb; 1.
DR   ProDom; PD008332; PTSIIB_sorb; 1.
DR   TIGRFAMs; TIGR00824; EIIA-man; 1.
DR   TIGRFAMs; TIGR00854; pts-sorbose; 1.
DR   PROSITE; PS51096; PTS_EIIA_TYPE_4; 1.
DR   PROSITE; PS51101; PTS_EIIB_TYPE_4; 1.
PE   3: Inferred from homology;
KW   Acetylation; Complete proteome; Cytoplasm; Kinase;
KW   Phosphotransferase system; Sugar transport; Transferase; Transport.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    323       PTS system mannose-specific EIIAB
FT                                component.
FT                                /FTId=PRO_0000186654.
FT   DOMAIN        2    124       PTS EIIA type-4.
FT   DOMAIN      157    320       PTS EIIB type-4.
FT   REGION      137    155       Hinge.
FT   ACT_SITE     10     10       Tele-phosphohistidine intermediate; for
FT                                EIIA activity (By similarity).
FT   ACT_SITE    175    175       Pros-phosphohistidine intermediate; for
FT                                EIIB activity (By similarity).
FT   MOD_RES      55     55       N6-acetyllysine (By similarity).
FT   MOD_RES     234    234       N6-acetyllysine (By similarity).
SQ   SEQUENCE   323 AA;  35048 MW;  A446B79421B8C040 CRC64;
     MTIAIVIGTH GWAAEQLLKT AEMLLGEQEN VGWIDFVPGE NAETLIEKYN AQLAKLDTTK
     GVLFLVDTWG GSPFNAASRI VVDKEHYEVI AGVNIPMLVE TLMARDDDPS FDELVALAVE
     TGREGVKALK AKPVEKAAPA PAAAAPKAAP TPAKPMGPND YMVIGLARID DRLIHGQVAT
     RWTKETNVSR IIVVSDEVAA DTVRKTLLTQ VAPPGVTAHV VDVAKMIRVY NNPKYAGERV
     MLLFTNPTDV ERLVEGGVKI TSVNVGGMAF RQGKTQVNNA VSVDEKDIEA FKKLNARGIE
     LEVRKVSTDP KLKMMDLISK IDK
//