N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component

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P69795 (PTQB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 63. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component
EC=2.7.1.69

Alternative name(s):
PTS system N,N'-diacetylchitobiose-specific EIIB component

Gene names

Name:	chbB
Synonyms:	celA
Ordered Locus Names:	b1738, JW1727

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	106 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in N,N'-diacetylchitobiose transport. Ref.7
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subunit structure	Monomer; in both its unphosphorylated and phosphorylated forms. Ref.9
Subcellular location	Cytoplasm.
Induction	By N,N'-diacetylchitobiose.
Domain	The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-3 domain.
Caution	Was originally (Ref.5) characterized as part of a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent and altering the substrate specificity.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 106

106

N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component

PRO_0000186489

Regions

Domain

3 – 106

104

PTS EIIB type-3

Sites

Active site

Phosphocysteine intermediate By similarity

Secondary structure

106

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P69795 [UniParc].

Last modified May 10, 2005. Version 1.
Checksum: DE4AADE300D18F53
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FASTA

106

11,427

        10         20         30         40         50         60 
MEKKHIYLFC SAGMSTSLLV SKMRAQAEKY EVPVIIEAFP ETLAGEKGQN ADVVLLGPQI 

        70         80         90        100 
AYMLPEIQRL LPNKPVEVID SLLYGKVDGL GVLKAAVAAI KKAAAN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization and nucleotide sequence of the cryptic cel operon of Escherichia coli K12." Parker L.L., Hall B.G. Genetics 124:455-471(1990) [PubMed: 2179047] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The cellobiose permease of Escherichia coli consists of three proteins and is homologous to the lactose permease of Staphylococcus aureus." Reizer J., Reizer A., Saier M.H. Jr. Res. Microbiol. 141:1061-1067(1990) [PubMed: 2092358] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Wild-type Escherichia coli grows on the chitin disaccharide, N,N'-diacetylchitobiose, by expressing the cel operon." Keyhani N.O., Roseman S. Proc. Natl. Acad. Sci. U.S.A. 94:14367-14371(1997) [PubMed: 9405618] [Abstract] Cited for: IDENTIFICATION OF CHB OPERON.
[7]	"The chitin disaccharide, N,N'-diacetylchitobiose, is catabolized by Escherichia coli and is transported/phosphorylated by the phosphoenolpyruvate:glycose phosphotransferase system." Keyhani N.O., Wang L.-X., Lee Y.C., Roseman S. J. Biol. Chem. 275:33084-33090(2000) [PubMed: 10913117] [Abstract] Cited for: FUNCTION.
[8]	"The transport/phosphorylation of N,N'-diacetylchitobiose in Escherichia coli. Characterization of phospho-IIB(Chb) and of a potential transition state analogue in the phosphotransfer reaction between the proteins IIA(Chb) and IIB(Chb)." Keyhani N.O., Bacia K., Roseman S. J. Biol. Chem. 275:33102-33109(2000) [PubMed: 10913119] [Abstract] Cited for: CHARACTERIZATION.
[9]	"Analytical sedimentation of the IIAChb and IIBChb proteins of the Escherichia coli N,N'-diacetylchitobiose phosphotransferase system. Demonstration of a model phosphotransfer transition state complex." Keyhani N.O., Rodgers M.E., Demeler B., Hansen J.C., Roseman S. J. Biol. Chem. 275:33110-33115(2000) [PubMed: 10913122] [Abstract] Cited for: SUBUNIT.
[10]	"Enzyme IIBcellobiose of the phosphoenol-pyruvate-dependent phosphotransferase system of Escherichia coli: backbone assignment and secondary structure determined by three-dimensional NMR spectroscopy." Ab E., Schuurman-Wolters G.K., Saier M.H. Jr., Reizer J., Jacuinod M., Roepstorff P., Dijkstra K., Scheek R.M., Robillard G.T. Protein Sci. 3:282-290(1994) [PubMed: 8003964] [Abstract] Cited for: STRUCTURE BY NMR.
[11]	"The NMR side-chain assignments and solution structure of enzyme IIBcellobiose of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli." Ab E., Schuurman-Wolters G.K., Reizer J., Saier M.H. Jr., Dijkstra K., Scheek R.M., Robillard G.T. Protein Sci. 6:304-314(1997) [PubMed: 9041631] [Abstract] Cited for: STRUCTURE BY NMR OF MUTANT SER-10.
[12]	"The structure of an energy-coupling protein from bacteria, IIBcellobiose, reveals similarity to eukaryotic protein tyrosine phosphatases." van Montfort R.L.M., Pijning T., Kalk K.H., Reizer J., Saier M.H. Jr., Thunnissen M.M.G.M., Robillard G.T., Dijkstra B.W. Structure 5:217-225(1997) [PubMed: 9032081] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X52890 Genomic DNA. Translation: CAA37069.1.
U00096 Genomic DNA. Translation: AAC74808.1.
AP009048 Genomic DNA. Translation: BAA15519.1.

PIR

S10870.

RefSeq

NP_416252.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1E2B	NMR	-	A	1-106	[»]
1H9C	NMR	-	A	1-106	[»]
1IIB	X-ray	1.80	A/B	1-106	[»]
2WWV	NMR	-	D	3-105	[»]
2WY2	NMR	-	D	3-105	[»]

ProteinModelPortal

P69795.

SMR

P69795. Positions 3-93.

ModBase

Search...

Protein-protein interaction databases

IntAct

P69795. 1 interaction.

Protein family/group databases

TCDB

4.A.3.2.1. PTS lactose-N,N'-diacetylchitobiose-beta,-glucoside (Lac) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000003020; EBESCP00000003020; EBESCG00000002476.
EBESCT00000017508; EBESCP00000016799; EBESCG00000016564.

GeneID

945339.

GenomeReviews

Gene locus JW1727 in contig AP009048_GR.
Gene locus b1738 in contig U00096_GR.

KEGG

ecj:JW1727.
eco:b1738.

PATRIC

32118785. VBIEscCol129921_1810.

Organism-specific databases

EchoBASE

EB0138.

EcoGene

EG10140. chbB.

Phylogenomic databases

eggNOG

COG1440.

GeneTree

EBGT00050000011047.

HOGENOM

HBG697211.

OMA

VFMEKKH.

PhylomeDB

P69795.

ProtClustDB

PRK10499.

Enzyme and pathway databases

BioCyc

EcoCyc:CELA-MONOMER.

Gene expression databases

Genevestigator

P69795.

Family and domain databases

InterPro

IPR014350. PTrfase_system_EIIB_3_subgr.
IPR003501. PTS_EIIB_2/3.
IPR013012. PTS_EIIB_3.
[Graphical view]

K02760.

Pfam

PF02302. PTS_IIB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00853. Pts-lac. 1 hit.

PROSITE

PS51100. PTS_EIIB_TYPE_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PTQB_ECOLI

Accession

Primary (citable) accession number: P69795
Secondary accession number(s): P17409

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 10, 2005
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents