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Protein

N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIA component

Gene

chbA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in N,N'-diacetylchitobiose transport.1 Publication

Catalytic activityi

Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891Tele-phosphohistidine intermediate; by HPrBy similarity

GO - Molecular functioni

GO - Biological processi

  • carbohydrate transmembrane transport Source: GOC
  • N,N'-diacetylchitobiose import Source: EcoCyc
  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
  • protein homooligomerization Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:CELC-MONOMER.
ECOL316407:JW1725-MONOMER.
MetaCyc:CELC-MONOMER.

Protein family/group databases

TCDBi4.A.3.2.1. the pts lactose-n,n'-diacetylchitobiose--glucoside (lac) family.

Names & Taxonomyi

Protein namesi
Recommended name:
N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIA component (EC:2.7.1.-)
Alternative name(s):
EIIA-Chb
EIII-Chb
PTS system N,N'-diacetylchitobiose-specific EIIA component
Gene namesi
Name:chbA
Synonyms:celC
Ordered Locus Names:b1736, JW1725
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10142. chbA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 116116N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIA componentPRO_0000186494Add
BLAST

Proteomic databases

PaxDbiP69791.

Expressioni

Inductioni

By N,N'-diacetylchitobiose.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4259134. 10 interactions.
DIPiDIP-9265N.
IntActiP69791. 11 interactions.
STRINGi511145.b1736.

Structurei

Secondary structure

1
116
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi15 – 4329Combined sources
Helixi47 – 7428Combined sources
Beta strandi77 – 793Combined sources
Helixi85 – 11430Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WCRNMR-A/B/C14-116[»]
2LRKNMR-A/B/C14-116[»]
2LRLNMR-A/B/C14-116[»]
2WWVNMR-A/B/C14-116[»]
2WY2NMR-A/B/C14-116[»]
ProteinModelPortaliP69791.
SMRiP69791. Positions 14-116.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69791.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 11399PTS EIIA type-3PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.

Sequence similaritiesi

Contains 1 PTS EIIA type-3 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105VCP. Bacteria.
COG1447. LUCA.
HOGENOMiHOG000224338.
InParanoidiP69791.
KOiK02759.
OMAiMEQSRMA.
OrthoDBiEOG6CGCMS.
PhylomeDBiP69791.

Family and domain databases

Gene3Di1.20.58.80. 1 hit.
InterProiIPR003188. PTS_IIA_lac.
[Graphical view]
PfamiPF02255. PTS_IIA. 1 hit.
[Graphical view]
PIRSFiPIRSF000699. PTS_IILac_III. 1 hit.
SUPFAMiSSF46973. SSF46973. 1 hit.
TIGRFAMsiTIGR00823. EIIA-LAC. 1 hit.
PROSITEiPS51095. PTS_EIIA_TYPE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69791-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMDLDNIPDT QTEAEELEEV VMGLIINSGQ ARSLAYAALK QAKQGDFAAA
60 70 80 90 100
KAMMDQSRMA LNEAHLVQTK LIEGDAGEGK MKVSLVLVHA QDHLMTSMLA
110
RELITELIEL HEKLKA
Length:116
Mass (Da):12,748
Last modified:May 10, 2005 - v1
Checksum:iF2951DC6700FA8A9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti43 – 442KQ → NR in CAA37071 (PubMed:2179047).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti14 – 141A → V in strain: ECOR 1.
Natural varianti52 – 521A → T in strain: ECOR 61.
Natural varianti59 – 591M → I in strain: ECOR 50.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52890 Genomic DNA. Translation: CAA37071.1.
M93570 Genomic DNA. Translation: AAA23551.1.
M93571 Genomic DNA. Translation: AAA23553.1.
M93572 Genomic DNA. Translation: AAA23554.1.
M93573 Genomic DNA. Translation: AAA23559.1.
M93574 Genomic DNA. Translation: AAA23556.1.
M93575 Genomic DNA. Translation: AAA23552.1.
M93576 Genomic DNA. Translation: AAA23560.1.
M93577 Genomic DNA. Translation: AAA23557.1.
M93591 Genomic DNA. Translation: AAA23561.1.
M93592 Genomic DNA. Translation: AAA23555.1.
M93593 Genomic DNA. Translation: AAA23558.1.
U00096 Genomic DNA. Translation: AAC74806.1.
AP009048 Genomic DNA. Translation: BAA15517.1.
PIRiH64932.
RefSeqiNP_416250.1. NC_000913.3.
WP_000968919.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74806; AAC74806; b1736.
BAA15517; BAA15517; BAA15517.
GeneIDi946244.
KEGGiecj:JW1725.
eco:b1736.
PATRICi32118781. VBIEscCol129921_1808.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52890 Genomic DNA. Translation: CAA37071.1.
M93570 Genomic DNA. Translation: AAA23551.1.
M93571 Genomic DNA. Translation: AAA23553.1.
M93572 Genomic DNA. Translation: AAA23554.1.
M93573 Genomic DNA. Translation: AAA23559.1.
M93574 Genomic DNA. Translation: AAA23556.1.
M93575 Genomic DNA. Translation: AAA23552.1.
M93576 Genomic DNA. Translation: AAA23560.1.
M93577 Genomic DNA. Translation: AAA23557.1.
M93591 Genomic DNA. Translation: AAA23561.1.
M93592 Genomic DNA. Translation: AAA23555.1.
M93593 Genomic DNA. Translation: AAA23558.1.
U00096 Genomic DNA. Translation: AAC74806.1.
AP009048 Genomic DNA. Translation: BAA15517.1.
PIRiH64932.
RefSeqiNP_416250.1. NC_000913.3.
WP_000968919.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WCRNMR-A/B/C14-116[»]
2LRKNMR-A/B/C14-116[»]
2LRLNMR-A/B/C14-116[»]
2WWVNMR-A/B/C14-116[»]
2WY2NMR-A/B/C14-116[»]
ProteinModelPortaliP69791.
SMRiP69791. Positions 14-116.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259134. 10 interactions.
DIPiDIP-9265N.
IntActiP69791. 11 interactions.
STRINGi511145.b1736.

Protein family/group databases

TCDBi4.A.3.2.1. the pts lactose-n,n'-diacetylchitobiose--glucoside (lac) family.

Proteomic databases

PaxDbiP69791.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74806; AAC74806; b1736.
BAA15517; BAA15517; BAA15517.
GeneIDi946244.
KEGGiecj:JW1725.
eco:b1736.
PATRICi32118781. VBIEscCol129921_1808.

Organism-specific databases

EchoBASEiEB0140.
EcoGeneiEG10142. chbA.

Phylogenomic databases

eggNOGiENOG4105VCP. Bacteria.
COG1447. LUCA.
HOGENOMiHOG000224338.
InParanoidiP69791.
KOiK02759.
OMAiMEQSRMA.
OrthoDBiEOG6CGCMS.
PhylomeDBiP69791.

Enzyme and pathway databases

BioCyciEcoCyc:CELC-MONOMER.
ECOL316407:JW1725-MONOMER.
MetaCyc:CELC-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP69791.
PROiP69791.

Family and domain databases

Gene3Di1.20.58.80. 1 hit.
InterProiIPR003188. PTS_IIA_lac.
[Graphical view]
PfamiPF02255. PTS_IIA. 1 hit.
[Graphical view]
PIRSFiPIRSF000699. PTS_IILac_III. 1 hit.
SUPFAMiSSF46973. SSF46973. 1 hit.
TIGRFAMsiTIGR00823. EIIA-LAC. 1 hit.
PROSITEiPS51095. PTS_EIIA_TYPE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization and nucleotide sequence of the cryptic cel operon of Escherichia coli K12."
    Parker L.L., Hall B.G.
    Genetics 124:455-471(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Molecular population genetics of Escherichia coli: DNA sequence diversity at the celC, crr, and gutB loci of natural isolates."
    Hall B.G., Sharp P.M.
    Mol. Biol. Evol. 9:654-665(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 28, 35, 37, 50, 51, 58, 6, 61, 66, 69 and ECOR 1.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Wild-type Escherichia coli grows on the chitin disaccharide, N,N'-diacetylchitobiose, by expressing the cel operon."
    Keyhani N.O., Roseman S.
    Proc. Natl. Acad. Sci. U.S.A. 94:14367-14371(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF CHB OPERON.
  7. "The chitin disaccharide, N,N'-diacetylchitobiose, is catabolized by Escherichia coli and is transported/phosphorylated by the phosphoenolpyruvate:glycose phosphotransferase system."
    Keyhani N.O., Wang L.-X., Lee Y.C., Roseman S.
    J. Biol. Chem. 275:33084-33090(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Isolation and characterization of IIAChb, a soluble protein of the enzyme II complex required for the transport/phosphorylation of N, N'-diacetylchitobiose in Escherichia coli."
    Keyhani N.O., Boudker O., Roseman S.
    J. Biol. Chem. 275:33091-33101(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  9. "Analytical sedimentation of the IIAChb and IIBChb proteins of the Escherichia coli N,N'-diacetylchitobiose phosphotransferase system. Demonstration of a model phosphotransfer transition state complex."
    Keyhani N.O., Rodgers M.E., Demeler B., Hansen J.C., Roseman S.
    J. Biol. Chem. 275:33110-33115(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.

Entry informationi

Entry nameiPTQA_ECOLI
AccessioniPrimary (citable) accession number: P69791
Secondary accession number(s): P17335
, Q47092, Q47093, Q47094, Q57128
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: January 20, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally (PubMed:2179047) characterized as part of a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent and altering the substrate specificity.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.