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Protein

PTS system N,N'-diacetylchitobiose-specific EIIA component

Gene

chbA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II ChbABC PTS system is involved in the transport of the chitin disaccharide N,N'-diacetylchitobiose (GlcNAc2) (PubMed:10913118, PubMed:10913117). Also able to use N,N',N''-triacetyl chitotriose (GlcNAc3) (PubMed:10913117).1 Publication2 Publications

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + N,N'-diacetylchitobiose(Side 1) = [protein]-L-histidine + N,N'-diacetylchitobiose 6'-phosphate(Side 2).2 Publications

Cofactori

Mg2+1 Publication1 PublicationNote: Can also use copper and nickel with lower efficiency.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei89Tele-phosphohistidine intermediate4 Publications1

GO - Molecular functioni

GO - Biological processi

  • N,N'-diacetylchitobiose import Source: EcoCyc
  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
  • protein homooligomerization Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:CELC-MONOMER.
ECOL316407:JW1725-MONOMER.
MetaCyc:CELC-MONOMER.

Protein family/group databases

TCDBi4.A.3.2.1. the pts lactose-n,n'-diacetylchitobiose-Beta-glucoside (lac) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system N,N'-diacetylchitobiose-specific EIIA component1 Publication
Alternative name(s):
EIIA-Chb1 Publication
EIII-Chb1 Publication
IIIcel1 Publication
N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIA component1 Publication (EC:2.7.1.1962 Publications)
Gene namesi
Name:chbA1 Publication
Synonyms:celC1 Publication
Ordered Locus Names:b1736, JW1725
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10142. chbA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi92D → L: Eliminates the need for a metal cation at the homotrimer interface by substituting hydrophobic methyl-methyl interactions in place of the metal cation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001864941 – 116PTS system N,N'-diacetylchitobiose-specific EIIA componentAdd BLAST116

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei89Phosphohistidine; by HPrPROSITE-ProRule annotation3 Publications1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP69791.
PRIDEiP69791.

Expressioni

Inductioni

By GlcNAc2, GlcNAc3 and beta-N,N'-diacetylchitobiose (Me-TCB).1 Publication

Interactioni

Subunit structurei

Forms a complex with ChbB (EIIB) (PubMed:10913119, PubMed:10913122, PubMed:19959833). ChbA is a homotrimer (PubMed:10913122, PubMed:15654077, PubMed:19959833, PubMed:22593574). The interface of the homotrimer is stabilized by metal cations such as magnesium, copper or nickel (PubMed:15654077).1 Publication5 Publications

Protein-protein interaction databases

BioGridi4259134. 10 interactors.
DIPiDIP-9265N.
IntActiP69791. 11 interactors.
STRINGi511145.b1736.

Structurei

Secondary structure

1116
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 43Combined sources29
Helixi47 – 74Combined sources28
Beta strandi77 – 79Combined sources3
Helixi85 – 114Combined sources30

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WCRNMR-A/B/C14-116[»]
2LRKNMR-A/B/C14-116[»]
2LRLNMR-A/B/C14-116[»]
2WWVNMR-A/B/C14-116[»]
2WY2NMR-A/B/C14-116[»]
ProteinModelPortaliP69791.
SMRiP69791.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69791.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 113PTS EIIA type-3PROSITE-ProRule annotationAdd BLAST99

Domaini

The PTS EIIA type-3 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-3 domain.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 PTS EIIA type-3 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105VCP. Bacteria.
COG1447. LUCA.
HOGENOMiHOG000224338.
InParanoidiP69791.
KOiK02759.
OMAiMEQSRMA.
PhylomeDBiP69791.

Family and domain databases

CDDicd00215. PTS_IIA_lac. 1 hit.
Gene3Di1.20.58.80. 1 hit.
InterProiIPR003188. PTS_IIA_lac/cel.
[Graphical view]
PfamiPF02255. PTS_IIA. 1 hit.
[Graphical view]
PIRSFiPIRSF000699. PTS_IILac_III. 1 hit.
SUPFAMiSSF46973. SSF46973. 1 hit.
TIGRFAMsiTIGR00823. EIIA-LAC. 1 hit.
PROSITEiPS51095. PTS_EIIA_TYPE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69791-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMDLDNIPDT QTEAEELEEV VMGLIINSGQ ARSLAYAALK QAKQGDFAAA
60 70 80 90 100
KAMMDQSRMA LNEAHLVQTK LIEGDAGEGK MKVSLVLVHA QDHLMTSMLA
110
RELITELIEL HEKLKA
Length:116
Mass (Da):12,748
Last modified:May 10, 2005 - v1
Checksum:iF2951DC6700FA8A9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti43 – 44KQ → NR in CAA37071 (PubMed:2179047).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti14A → V in strain: ECOR 1. 1
Natural varianti52A → T in strain: ECOR 61. 1
Natural varianti59M → I in strain: ECOR 50. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52890 Genomic DNA. Translation: CAA37071.1.
M93570 Genomic DNA. Translation: AAA23551.1.
M93571 Genomic DNA. Translation: AAA23553.1.
M93572 Genomic DNA. Translation: AAA23554.1.
M93573 Genomic DNA. Translation: AAA23559.1.
M93574 Genomic DNA. Translation: AAA23556.1.
M93575 Genomic DNA. Translation: AAA23552.1.
M93576 Genomic DNA. Translation: AAA23560.1.
M93577 Genomic DNA. Translation: AAA23557.1.
M93591 Genomic DNA. Translation: AAA23561.1.
M93592 Genomic DNA. Translation: AAA23555.1.
M93593 Genomic DNA. Translation: AAA23558.1.
U00096 Genomic DNA. Translation: AAC74806.1.
AP009048 Genomic DNA. Translation: BAA15517.1.
PIRiH64932.
RefSeqiNP_416250.1. NC_000913.3.
WP_000968919.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74806; AAC74806; b1736.
BAA15517; BAA15517; BAA15517.
GeneIDi946244.
KEGGiecj:JW1725.
eco:b1736.
PATRICi32118781. VBIEscCol129921_1808.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52890 Genomic DNA. Translation: CAA37071.1.
M93570 Genomic DNA. Translation: AAA23551.1.
M93571 Genomic DNA. Translation: AAA23553.1.
M93572 Genomic DNA. Translation: AAA23554.1.
M93573 Genomic DNA. Translation: AAA23559.1.
M93574 Genomic DNA. Translation: AAA23556.1.
M93575 Genomic DNA. Translation: AAA23552.1.
M93576 Genomic DNA. Translation: AAA23560.1.
M93577 Genomic DNA. Translation: AAA23557.1.
M93591 Genomic DNA. Translation: AAA23561.1.
M93592 Genomic DNA. Translation: AAA23555.1.
M93593 Genomic DNA. Translation: AAA23558.1.
U00096 Genomic DNA. Translation: AAC74806.1.
AP009048 Genomic DNA. Translation: BAA15517.1.
PIRiH64932.
RefSeqiNP_416250.1. NC_000913.3.
WP_000968919.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WCRNMR-A/B/C14-116[»]
2LRKNMR-A/B/C14-116[»]
2LRLNMR-A/B/C14-116[»]
2WWVNMR-A/B/C14-116[»]
2WY2NMR-A/B/C14-116[»]
ProteinModelPortaliP69791.
SMRiP69791.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259134. 10 interactors.
DIPiDIP-9265N.
IntActiP69791. 11 interactors.
STRINGi511145.b1736.

Protein family/group databases

TCDBi4.A.3.2.1. the pts lactose-n,n'-diacetylchitobiose-Beta-glucoside (lac) family.

Proteomic databases

PaxDbiP69791.
PRIDEiP69791.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74806; AAC74806; b1736.
BAA15517; BAA15517; BAA15517.
GeneIDi946244.
KEGGiecj:JW1725.
eco:b1736.
PATRICi32118781. VBIEscCol129921_1808.

Organism-specific databases

EchoBASEiEB0140.
EcoGeneiEG10142. chbA.

Phylogenomic databases

eggNOGiENOG4105VCP. Bacteria.
COG1447. LUCA.
HOGENOMiHOG000224338.
InParanoidiP69791.
KOiK02759.
OMAiMEQSRMA.
PhylomeDBiP69791.

Enzyme and pathway databases

BioCyciEcoCyc:CELC-MONOMER.
ECOL316407:JW1725-MONOMER.
MetaCyc:CELC-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP69791.
PROiP69791.

Family and domain databases

CDDicd00215. PTS_IIA_lac. 1 hit.
Gene3Di1.20.58.80. 1 hit.
InterProiIPR003188. PTS_IIA_lac/cel.
[Graphical view]
PfamiPF02255. PTS_IIA. 1 hit.
[Graphical view]
PIRSFiPIRSF000699. PTS_IILac_III. 1 hit.
SUPFAMiSSF46973. SSF46973. 1 hit.
TIGRFAMsiTIGR00823. EIIA-LAC. 1 hit.
PROSITEiPS51095. PTS_EIIA_TYPE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTQA_ECOLI
AccessioniPrimary (citable) accession number: P69791
Secondary accession number(s): P17335
, Q47092, Q47093, Q47094, Q57128
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: November 30, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally (PubMed:2179047) characterized as part of a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent and altering the substrate specificity.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.