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Reviewed, UniProtKB/Swiss-Prot P69787 (PTGCB_ECOL6)

Last modified June 16, 2009. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    PTS system glucose-specific EIICB component
Alternative name(s):
    EIICB-Glc
      Short name=EII-Glc
Including the following 2 domains:
    1- Recommended name:
            Glucose permease IIC component
        Alternative name(s):
            PTS system glucose-specific EIIC component
    2- Recommended name:
            Glucose-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system glucose-specific EIIB component
Gene names
Name: ptsG
Ordered Locus Names: c1373
OrganismEscherichia coli O6 [Complete proteome] [HAMAP]
Taxonomic identifier217992 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length477 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport. This enzyme is also a chemoreceptor monitoring the environment for changes in sugar concentration By similarity.

Catalytic activity

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.

Subcellular location

Cell inner membrane; Multi-pass membrane protein By similarity.

Domain

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

Sequence similarities

Contains 1 PTS EIIB type-1 domain.

Contains 1 PTS EIIC type-1 domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 477477PTS system glucose-specific EIICB component
PRO_0000186529

Regions

Topological domain1 – 1414Cytoplasmic Potential
Transmembrane15 – 3521 Potential
Topological domain36 – 5015Periplasmic Potential
Transmembrane51 – 7121 Potential
Topological domain72 – 798Cytoplasmic Potential
Transmembrane80 – 10021 Potential
Topological domain101 – 11111Periplasmic Potential
Transmembrane112 – 13221 Potential
Topological domain133 – 15119Cytoplasmic Potential
Transmembrane152 – 17221 Potential
Topological domain173 – 19018Periplasmic Potential
Transmembrane191 – 21121 Potential
Topological domain212 – 24938Cytoplasmic Potential
Transmembrane250 – 27021 Potential
Topological domain271 – 2799Periplasmic Potential
Transmembrane280 – 30021 Potential
Topological domain301 – 3099Cytoplasmic Potential
Transmembrane310 – 33021 Potential
Topological domain331 – 35525Periplasmic Potential
Transmembrane356 – 37621 Potential
Topological domain377 – 477101Cytoplasmic Potential
Domain1 – 388388PTS EIIC type-1
Domain399 – 47779PTS EIIB type-1

Sites

Active site4211Phosphocysteine intermediate; for EIIB activity By similarity

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Sequences

Sequence LengthMass (Da)Tools
P69787-1 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: D97A80FD64B74F73

FASTA47750,677
        10         20         30         40         50         60 
MFKNAFANLQ KVGKSLMLPV SVLPIAGILL GVGSANFSWL PAVVSHVMAE AGGSVFANMP 

        70         80         90        100        110        120 
LIFAIGVALG FTNNDGVSAL AAVVAYGIMV KTMAVVAPLV LHLPAEEIAS KHLADTGVLG 

       130        140        150        160        170        180 
GIISGAIAAY MFNRFYRIKL PEYLGFFAGK RFVPIISGLA AIFTGVVLSF IWPPIGSAIQ 

       190        200        210        220        230        240 
TFSQWAAYQN PVVAFGIYGF IERCLVPFGL HHIWNVPFQM QIGEYTNAAG QVFHGDIPRY 

       250        260        270        280        290        300 
MAGDPTAGKL SGGFLFKMYG LPAAAIAIWH SAKPENRAKV GGIMISAALT SFLTGITEPI 

       310        320        330        340        350        360 
EFSFMFVAPI LYIIHAILAG LAFPICILLG MRDGTSFSHG LIDFIVLSGN SSKLWLFPIV 

       370        380        390        400        410        420 
GIGYAIVYYT IFRVLIKALD LKTPGREDAT EDAKATGTSE MAPALVAAFG GKENITNLDA 

       430        440        450        460        470 
CITRLRVSVA DVSKVDQAGL KKLGAAGVVV AGSGVQAIFG TKSDNLKTEM DEYIRNH

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References

[1]"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli."
Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R.
Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed: 12471157] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O6:H1 / CFT073 / ATCC 700928 / UPEC.

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Cross-references

Sequence databases

AE014075 Genomic DNA. Translation: AAN79843.1.
RefSeqNP_753283.1.

3D structure databases

SMRP69787. Positions 400-476.
ModBaseSearch...

Genome annotation databases

GeneID1035072.
GenomeReviewsGene locus c1373 in contig AE014075_GR.
KEGGecc:c1373.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP69787.
OMAP69787. NNDGVAG.

Enzyme and pathway databases

BRENDA2.7.1.69. 292881.

Family and domain databases

InterProIPR018113. PTrfase_EIIB/Cys_phospho_site.
IPR001996. PTS_EIIB.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR011535. PTS_Glc-like_IIB_component.
IPR011299. PTS_IIBC_glc.
IPR004719. PTS_IIC_glc.
[Graphical view]
Gene3DG3DSA:3.30.1360.60. PTS_EIIB. 1 hit.
PfamPF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
TIGRFAMsTIGR00826. EIIB_glc. 1 hit.
TIGR00852. pts-Glc. 1 hit.
TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITEPS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePTGCB_ECOL6
AccessionPrimary (citable) accession number: P69787
Secondary accession number(s): P05053
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents