PTS system glucose-specific EIICB component - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P69786 (PTGCB_ECOLI)

Last modified November 3, 2009. Version 51. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    PTS system glucose-specific EIICB component
Alternative name(s):
    EIICB-Glc
      Short name=EII-Glc
Including the following 2 domains:
    1- Recommended name:
            Glucose permease IIC component
        Alternative name(s):
            PTS system glucose-specific EIIC component
    2- Recommended name:
            Glucose-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system glucose-specific EIIB component

Gene names

Name:	ptsG
Synonyms:	glcA, umg
Ordered Locus Names:	b1101, JW1087

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport. This enzyme is also a chemoreceptor monitoring the environment for changes in sugar concentration.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Enzyme regulation	Transporter activity may be inhibited by sgrT. Ref.8
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Induction	The ptsG transcript is degraded under conditions of glucose-phosphate stress (due to intracellular accumulation of glucose-6-phosphate caused by disruption of glycolytic flux), or in the presence of (toxic) non-metabolizable glucose phosphate analogs due to hybridization with the small RNA sgrS (originally known as ryaA). This hybridization is sufficient to repress mRNA translation (Ref.8). The hybrid is subsequently degraded by RNase E. This reduces the quantity of transporter and relieves glucose phosphate stress. Ref.6
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-1 domain. Contains 1 PTS EIIC type-1 domain.

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Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	glucose transport Inferred from electronic annotation. Source: InterPro phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucose transmembrane transporter activity Inferred from electronic annotation. Source: InterPro kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
crr	P69783	1	EBI-903497,EBI-369856

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 477

477

PTS system glucose-specific EIICB component

PRO_0000186528

Regions

Topological domain

1 – 14

Cytoplasmic Potential

Transmembrane

15 – 35

Potential

Topological domain

36 – 50

Periplasmic Potential

Transmembrane

51 – 71

Potential

Topological domain

72 – 79

Cytoplasmic Potential

Transmembrane

80 – 100

Potential

Topological domain

101 – 111

Periplasmic Potential

Transmembrane

112 – 132

Potential

Topological domain

133 – 151

Cytoplasmic Potential

Transmembrane

152 – 172

Potential

Topological domain

173 – 190

Periplasmic Potential

Transmembrane

191 – 211

Potential

Topological domain

212 – 249

Cytoplasmic Potential

Transmembrane

250 – 270

Potential

Topological domain

271 – 279

Periplasmic Potential

Transmembrane

280 – 300

Potential

Topological domain

301 – 309

Cytoplasmic Potential

Transmembrane

310 – 330

Potential

Topological domain

331 – 355

Periplasmic Potential

Transmembrane

356 – 376

Potential

Topological domain

377 – 477

101

Cytoplasmic Potential

Domain

1 – 388

388

PTS EIIC type-1

Domain

399 – 477

PTS EIIB type-1

Sites

Active site

421

Phosphocysteine intermediate; for EIIB activity

Secondary structure

477

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P69786-1 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: D97A80FD64B74F73
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FASTA

477

50,677

        10         20         30         40         50         60 
MFKNAFANLQ KVGKSLMLPV SVLPIAGILL GVGSANFSWL PAVVSHVMAE AGGSVFANMP 

        70         80         90        100        110        120 
LIFAIGVALG FTNNDGVSAL AAVVAYGIMV KTMAVVAPLV LHLPAEEIAS KHLADTGVLG 

       130        140        150        160        170        180 
GIISGAIAAY MFNRFYRIKL PEYLGFFAGK RFVPIISGLA AIFTGVVLSF IWPPIGSAIQ 

       190        200        210        220        230        240 
TFSQWAAYQN PVVAFGIYGF IERCLVPFGL HHIWNVPFQM QIGEYTNAAG QVFHGDIPRY 

       250        260        270        280        290        300 
MAGDPTAGKL SGGFLFKMYG LPAAAIAIWH SAKPENRAKV GGIMISAALT SFLTGITEPI 

       310        320        330        340        350        360 
EFSFMFVAPI LYIIHAILAG LAFPICILLG MRDGTSFSHG LIDFIVLSGN SSKLWLFPIV 

       370        380        390        400        410        420 
GIGYAIVYYT IFRVLIKALD LKTPGREDAT EDAKATGTSE MAPALVAAFG GKENITNLDA 

       430        440        450        460        470 
CITRLRVSVA DVSKVDQAGL KKLGAAGVVV AGSGVQAIFG TKSDNLKTEM DEYIRNH

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Glucose permease of the bacterial phosphotransferase system. Gene cloning, overproduction, and amino acid sequence of enzyme IIGlc." Erni B., Zanolari B. J. Biol. Chem. 261:16398-16403(1986) [PubMed: 3023349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-16. Strain: K12.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Cysteine phosphorylation of the glucose transporter of Escherichia coli." Meins M., Jenoe P., Mueller D., Richter W.J., Rosenbusch J.P., Erni B. J. Biol. Chem. 268:11604-11609(1993) [PubMed: 8505292] [Abstract] Cited for: PHOSPHORYLATION AT CYS-421.
[6]	"Involvement of a novel transcriptional activator and small RNA in post-transcriptional regulation of the glucose phosphoenolpyruvate phosphotransferase system." Vanderpool C.K., Gottesman S. Mol. Microbiol. 54:1076-1089(2004) [PubMed: 15522088] [Abstract] Cited for: INDUCTION BY POST-TRANSCRIPTIONAL RNA REGULATION. Strain: K12 / MG1655 / ATCC 47076.
[7]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
[8]	"Translational repression is sufficient for gene silencing by bacterial small noncoding RNAs in the absence of mRNA destruction." Morita T., Mochizuki Y., Aiba H. Proc. Natl. Acad. Sci. U.S.A. 103:4858-4863(2006) [PubMed: 16549791] [Abstract] Cited for: ENZYME REGULATION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[9]	"The glucose transporter of Escherichia coli. Assignment of the 1H, 13C and 15N resonances and identification of the secondary structure of the soluble IIB domain." Golic Grdadolnik S., Eberstadt M., Gemmecker G., Kessler H., Buhr A., Erni B. Eur. J. Biochem. 219:945-952(1994) [PubMed: 8112346] [Abstract] Cited for: STRUCTURE BY NMR OF 391-476.
[10]	"Solution structure of the IIB domain of the glucose transporter of Escherichia coli." Eberstadt M., Golic Grdadolnik S., Gemmecker G., Kessler H., Buhr A., Erni B. Biochemistry 35:11286-11292(1996) [PubMed: 8784182] [Abstract] Cited for: STRUCTURE BY NMR OF 386-477.
[11]	"Glucose transporter of Escherichia coli: NMR characterization of the phosphocysteine form of the IIB(Glc) domain and its binding interface with the IIA(Glc) subunit." Gemmecker G., Eberstadt M., Buhr A., Lanz R., Golic Grdadolnik S., Kessler H., Erni B. Biochemistry 36:7408-7417(1997) [PubMed: 9200688] [Abstract] Cited for: STRUCTURE BY NMR OF 386-477.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

J02618 Genomic DNA. Translation: AAA24437.1.
U00096 Genomic DNA. Translation: AAC74185.1.
AP009048 Genomic DNA. Translation: BAA35908.1.

PIR

WQEC2G. A25336.

RefSeq

AP_001727.1.
NP_415619.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IBA	NMR	-	A	391-476	[»]
1O2F	NMR	-	B	387-476	[»]
3BP3	X-ray	1.65	A/B	396-477	[»]
3BP8	X-ray	2.85	C/D	401-475	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P69786. 1 interaction.

STRING

P69786.

Protein family/group databases

TCDB

4.A.1.1.1. PTS glucose-glucoside (Glc) family.

2-D gel databases

ECO2DBASE

H042.6. 6TH EDITION.
H042.7. 6TH EDITION.

Proteomic databases

PRIDE

P69786.

Genome annotation databases

GeneID

945651.

GenomeReviews

Gene locus JW1087 in contig AP009048_GR.
Gene locus b1101 in contig U00096_GR.

KEGG

ecj:JW1087.
eco:b1101.

Organism-specific databases

EchoBASE

EB0780.

EcoGene

EG10787. ptsG.

CMR

Search...

Phylogenomic databases

HOGENOM

P69786.

OMA

NNDGVAG.

Enzyme and pathway databases

BioCyc

EcoCyc:PTSG-MON.

Gene expression databases

Genevestigator

P69786.

Family and domain databases

InterPro

IPR018113. PTrfase_EIIB/Cys_phosph_CS.
IPR004719. PTrfase_sys_maltose/Glc-sp_IIC.
IPR001996. PTS_EIIB.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR011535. PTS_Glc-like_IIB_component.
IPR011299. PTS_IIBC_glc.
[Graphical view]

Gene3D

G3DSA:3.30.1360.60. PTS_EIIB. 1 hit.

Pfam

PF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00826. EIIB_glc. 1 hit.
TIGR00852. pts-Glc. 1 hit.
TIGR02002. PTS-II-BC-glcB. 1 hit.

PROSITE

PS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

PTGCB_ECOLI

Accession

Primary (citable) accession number: P69786
Secondary accession number(s): P05053

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	November 3, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

2-D gel databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents