##gff-version 3
P69776	UniProtKB	Signal peptide	1	20	.	.	.	Ontology_term=ECO:0000255,ECO:0000269,ECO:0000269;evidence=ECO:0000255|HAMAP-Rule:MF_00843,ECO:0000269|PubMed:4261992,ECO:0000269|PubMed:4575979;Dbxref=PMID:4261992,PMID:4575979	
P69776	UniProtKB	Chain	21	78	.	.	.	ID=PRO_0000018331;Note=Major outer membrane lipoprotein Lpp;Ontology_term=ECO:0000255;evidence=ECO:0000255|HAMAP-Rule:MF_00843	
P69776	UniProtKB	Repeat	24	34	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|HAMAP-Rule:MF_00843	
P69776	UniProtKB	Repeat	38	48	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|HAMAP-Rule:MF_00843	
P69776	UniProtKB	Coiled coil	27	75	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|HAMAP-Rule:MF_00843	
P69776	UniProtKB	Modified residue	1	1	.	.	.	Note=N-formylmethionine;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:322142;Dbxref=PMID:322142	
P69776	UniProtKB	Modified residue	78	78	.	.	.	Note=N6-murein peptidoglycan lysine;Ontology_term=ECO:0000255,ECO:0000269,ECO:0000269;evidence=ECO:0000255|HAMAP-Rule:MF_00843,ECO:0000269|PubMed:4245367,ECO:0000269|PubMed:4261992;Dbxref=PMID:4245367,PMID:4261992	
P69776	UniProtKB	Lipidation	21	21	.	.	.	Note=N-palmitoyl cysteine;Ontology_term=ECO:0000255,ECO:0000269;evidence=ECO:0000255|HAMAP-Rule:MF_00843,ECO:0000269|PubMed:4575979;Dbxref=PMID:4575979	
P69776	UniProtKB	Lipidation	21	21	.	.	.	Note=S-diacylglycerol cysteine;Ontology_term=ECO:0000255,ECO:0000269;evidence=ECO:0000255|HAMAP-Rule:MF_00843,ECO:0000269|PubMed:4575979;Dbxref=PMID:4575979	
P69776	UniProtKB	Natural variant	29	29	.	.	.	Note=In strain: B. Q->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:4261992;Dbxref=PMID:4261992	
P69776	UniProtKB	Mutagenesis	22	22	.	.	.	Note=Localizes to the inner membrane%2C where it associates with peptidoglycan%2C thereby preventing separation of the two membranes. Prolonged expression is lethal for the cell. S->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9139900;Dbxref=PMID:9139900	
P69776	UniProtKB	Mutagenesis	37	57	.	.	.	Note=Does not affect the formation of murein-bound lipoprotein%2C has almost no surface-exposed Lpp. Cells are mucoid. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21219470;Dbxref=PMID:21219470	
P69776	UniProtKB	Mutagenesis	43	43	.	.	.	Note=Periplasm is 4 nm larger than wild-type%2C cells no longer trigger Rcs system. Q->TLSAKVEQLSNDVNAMRSDVDQ;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:29257832;Dbxref=PMID:29257832	
P69776	UniProtKB	Mutagenesis	43	43	.	.	.	Note=Periplasm is 3 nm larger than wild-type%2C cells no longer trigger Rcs system. Q->TLSAKVEQLSNDVNQ;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:29257832;Dbxref=PMID:29257832	
P69776	UniProtKB	Mutagenesis	70	70	.	.	.	Note=Does not affect the formation of murein-bound lipoprotein. D->E%2CG%2CS;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:1527073;Dbxref=PMID:1527073	
P69776	UniProtKB	Mutagenesis	75	78	.	.	.	Note=Protein is no longer biotinylated on the cell surface. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21219470;Dbxref=PMID:21219470	
P69776	UniProtKB	Mutagenesis	75	75	.	.	.	Note=Does not affect the formation of murein-bound lipoprotein. K->S%2CT;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:1527073;Dbxref=PMID:1527073	
P69776	UniProtKB	Mutagenesis	76	76	.	.	.	Note=Reduces the formation of murein-bound lipoprotein. Y->C%2CD%2CE%2CG%2CN%2CP%2CS;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:1527073;Dbxref=PMID:1527073	
P69776	UniProtKB	Mutagenesis	76	76	.	.	.	Note=Does not affect the formation of murein-bound lipoprotein. Y->F%2CH%2CI%2CL;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:1527073;Dbxref=PMID:1527073	
P69776	UniProtKB	Mutagenesis	77	77	.	.	.	Note=Reduces the formation of murein-bound lipoprotein. R->D%2CL;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:1527073;Dbxref=PMID:1527073	
P69776	UniProtKB	Mutagenesis	78	78	.	.	.	Note=Abolishes the formation of murein-bound lipoprotein. Reduced biotinylation on the cell surface. K->R;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:1527073,ECO:0000269|PubMed:21219470;Dbxref=PMID:1527073,PMID:21219470	
P69776	UniProtKB	Mutagenesis	78	78	.	.	.	Note=Cells no longer trigger Rcs system%2C outer membrane forms blebs%2C periplasm is about 3 nm larger than wild-type. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:29257832;Dbxref=PMID:29257832	
P69776	UniProtKB	Helix	25	71	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1JCD