Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P69771 (DID2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar protein-sorting-associated protein 46
Alternative name(s):
Charged multivesicular body protein 1
DOA4-independent degradation protein 2
Fifty two inhibitor 1
Gene names
Name:DID2
Synonyms:CHM1, FTI1, VPS46
Ordered Locus Names:YKR035W-A
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length204 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Class E VPS protein implicated in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins will be targeted into the vacuole after fusion of the endosome with the vacuole. Probably acts as a peripherally associated component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruits late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. Regulates the membrane association of VPS4. Can stimulate VPS4 ATPase activity directly or via VTA1. Ref.3 Ref.4 Ref.7 Ref.8 Ref.10 Ref.12 Ref.13

Subunit structure

Self-associates. Interacts with VPS4 and VTA1. Interacts with IST1. Ref.7 Ref.8 Ref.10 Ref.11 Ref.12 Ref.13

Subcellular location

Endosome membrane; Peripheral membrane protein. Endomembrane system; Peripheral membrane protein. Note: Endosomal and other punctate structures. Ref.3 Ref.5

Miscellaneous

Present with 2440 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SNF7 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 204204Vacuolar protein-sorting-associated protein 46
PRO_0000211461

Regions

Region1 – 103103Interaction with VSP24
Region104 – 204101Interaction with VSP4
Region176 – 20429Interaction with VTA1
Coiled coil9 – 5648 Potential
Coiled coil109 – 12921 Potential

Amino acid modifications

Modified residue51Phosphoserine Ref.14

Experimental info

Mutagenesis1981R → D: Impairs sorting. Ref.11
Mutagenesis1991L → D: Impairs sorting; when associated with D-202. Ref.11
Mutagenesis2021L → D: Impairs sorting; when associated with D-199. Ref.11

Sequences

Sequence LengthMass (Da)Tools
P69771 [UniParc].

Last modified April 26, 2005. Version 1.
Checksum: 70D1CF4588A8C6E6

FASTA20423,091
        10         20         30         40         50         60 
MSRNSAAGLE NTLFQLKFTS KQLQKQANKA SKEEKQETNK LKRALNENED ISRIYASNAI 

        70         80         90        100        110        120 
RKKNERLQLL KLASRVDSVA SRVQTAVTMR QVSASMGQVC KGMDKALQNM NLQQITMIMD 

       130        140        150        160        170        180 
KFEQQFEDLD TSVNVYEDMG VNSDAMLVDN DKVDELMSKV ADENGMELKQ SAKLDNVPEI 

       190        200 
KAKEVNVDDE KEDKLAQRLR ALRG 

« Hide

References

« Hide 'large scale' references
[1]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways."
Amerik A.Y., Nowak J., Swaminathan S., Hochstrasser M.
Mol. Biol. Cell 11:3365-3380(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]"CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins."
Howard T.L., Stauffer D.R., Degnin C.R., Hollenberg S.M.
J. Cell Sci. 114:2395-2404(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae."
Bowers K., Lottridge J., Helliwell S.B., Goldthwaite L.M., Luzio J.P., Stevens T.H.
Traffic 5:194-210(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SELF-ASSOCIATION, INTERACTION WITH VPS4 AND VTA1.
[8]"Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes."
Nickerson D.P., West M., Odorizzi G.
J. Cell Biol. 175:715-720(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH VPS4 AND VPS24.
[9]Erratum
Nickerson D.P., West M., Odorizzi G.
J. Cell Biol. 175:1043-1043(2006)
[10]"Vta1p and Vps46p regulate the membrane association and ATPase activity of Vps4p at the yeast multivesicular body."
Lottridge J.M., Flannery A.R., Vincelli J.L., Stevens T.H.
Proc. Natl. Acad. Sci. U.S.A. 103:6202-6207(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH VSP4 AND VTA1.
[11]"Structural basis for selective recognition of ESCRT-III by the AAA ATPase Vps4."
Obita T., Saksena S., Ghazi-Tabatabai S., Gill D.J., Perisic O., Emr S.D., Williams R.L.
Nature 449:735-739(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH VSP4, MUTAGENESIS OF ARG-198; LEU-199 AND LEU-202.
[12]"ESCRT-III family members stimulate Vps4 ATPase activity directly or via Vta1."
Azmi I.F., Davies B.A., Xiao J., Babst M., Xu Z., Katzmann D.J.
Dev. Cell 14:50-61(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH VTA1.
[13]"Novel Ist1-Did2 complex functions at a late step in multivesicular body sorting."
Rue S.M., Mattei S., Saksena S., Emr S.D.
Mol. Biol. Cell 19:475-484(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH IST1.
[14]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z28260 Genomic DNA. No translation available.
BK006944 Genomic DNA. Translation: DAA09188.1.
RefSeqNP_012961.3. NM_001184309.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3GGZX-ray3.80E/F/G/H176-204[»]
ProteinModelPortalP69771.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34166. 81 interactions.
DIPDIP-52223N.
IntActP69771. 4 interactions.
MINTMINT-4493543.
STRING4932.YKR035W-A.

Proteomic databases

MaxQBP69771.
PaxDbP69771.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKR035W-A; YKR035W-A; YKR035W-A.
GeneID853906.
KEGGsce:YKR035W-A.

Organism-specific databases

CYGDYKR035w-a.
SGDS000006435. DID2.

Phylogenomic databases

eggNOGCOG5491.
GeneTreeENSGT00550000074957.
HOGENOMHOG000241980.
KOK12197.
OrthoDBEOG77DJJM.

Enzyme and pathway databases

BioCycYEAST:G3O-32071-MONOMER.

Gene expression databases

GenevestigatorP69771.

Family and domain databases

InterProIPR005024. Snf7.
[Graphical view]
PfamPF03357. Snf7. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP69771.
NextBio975236.

Entry information

Entry nameDID2_YEAST
AccessionPrimary (citable) accession number: P69771
Secondary accession number(s): D6VX98
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: June 11, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references