Vacuolar protein-sorting-associated protein 46 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P69771 (DID2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 76. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Vacuolar protein-sorting-associated protein 46

Alternative name(s):
Charged multivesicular body protein 1
DOA4-independent degradation protein 2
Fifty two inhibitor 1

Gene names

Name:	DID2
Synonyms:	CHM1, FTI1, VPS46
Ordered Locus Names:	YKR035W-A

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Class E VPS protein implicated in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins will be targeted into the vacuole after fusion of the endosome with the vacuole. Probably acts as a peripherally associated component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruits late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. Regulates the membrane association of VPS4. Can stimulate VPS4 ATPase activity directly or via VTA1. Ref.3 Ref.4 Ref.7 Ref.8 Ref.10 Ref.12 Ref.13
Subunit structure	Self-associates. Interacts with VPS4 and VTA1. Interacts with IST1. Ref.7 Ref.8 Ref.10 Ref.11 Ref.12 Ref.13
Subcellular location	Endosome membrane; Peripheral membrane protein. Endomembrane system; Peripheral membrane protein. Note: Endosomal and other punctate structures. Ref.3 Ref.5
Miscellaneous	Present with 2440 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SNF7 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Endosome Membrane
Domain	Coiled coil
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	late endosome to vacuole transport via multivesicular body sorting pathway Inferred from mutant phenotype Ref.8. Source: SGD protein targeting to vacuole Inferred from mutant phenotype Ref.3. Source: SGD
Cellular_component	endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell late endosome Inferred from direct assay Ref.3. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 204

204

Vacuolar protein-sorting-associated protein 46

PRO_0000211461

Regions

Region

1 – 103

103

Interaction with VSP24

Region

104 – 204

101

Interaction with VSP4

Region

176 – 204

Interaction with VTA1

Coiled coil

9 – 56

Potential

Coiled coil

109 – 129

Potential

Experimental info

Mutagenesis

198

R → D: Impairs sorting. Ref.11

Mutagenesis

199

L → D: Impairs sorting; when associated with D-202. Ref.11

Mutagenesis

202

L → D: Impairs sorting; when associated with D-199. Ref.11

Sequences

Sequence

Length

Mass (Da)

Tools

P69771 [UniParc].

Last modified April 26, 2005. Version 1.
Checksum: 70D1CF4588A8C6E6
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FASTA

204

23,091

        10         20         30         40         50         60 
MSRNSAAGLE NTLFQLKFTS KQLQKQANKA SKEEKQETNK LKRALNENED ISRIYASNAI 

        70         80         90        100        110        120 
RKKNERLQLL KLASRVDSVA SRVQTAVTMR QVSASMGQVC KGMDKALQNM NLQQITMIMD 

       130        140        150        160        170        180 
KFEQQFEDLD TSVNVYEDMG VNSDAMLVDN DKVDELMSKV ADENGMELKQ SAKLDNVPEI 

       190        200 
KAKEVNVDDE KEDKLAQRLR ALRG

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways." Amerik A.Y., Nowak J., Swaminathan S., Hochstrasser M. Mol. Biol. Cell 11:3365-3380(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]	"CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins." Howard T.L., Stauffer D.R., Degnin C.R., Hollenberg S.M. J. Cell Sci. 114:2395-2404(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae." Bowers K., Lottridge J., Helliwell S.B., Goldthwaite L.M., Luzio J.P., Stevens T.H. Traffic 5:194-210(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SELF-ASSOCIATION, INTERACTION WITH VPS4 AND VTA1.
[8]	"Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes." Nickerson D.P., West M., Odorizzi G. J. Cell Biol. 175:715-720(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH VPS4 AND VPS24.
[9]	Erratum Nickerson D.P., West M., Odorizzi G. J. Cell Biol. 175:1043-1043(2006)
[10]	"Vta1p and Vps46p regulate the membrane association and ATPase activity of Vps4p at the yeast multivesicular body." Lottridge J.M., Flannery A.R., Vincelli J.L., Stevens T.H. Proc. Natl. Acad. Sci. U.S.A. 103:6202-6207(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH VSP4 AND VTA1.
[11]	"Structural basis for selective recognition of ESCRT-III by the AAA ATPase Vps4." Obita T., Saksena S., Ghazi-Tabatabai S., Gill D.J., Perisic O., Emr S.D., Williams R.L. Nature 449:735-739(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH VSP4, MUTAGENESIS OF ARG-198; LEU-199 AND LEU-202.
[12]	"ESCRT-III family members stimulate Vps4 ATPase activity directly or via Vta1." Azmi I.F., Davies B.A., Xiao J., Babst M., Xu Z., Katzmann D.J. Dev. Cell 14:50-61(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH VTA1.
[13]	"Novel Ist1-Did2 complex functions at a late step in multivesicular body sorting." Rue S.M., Mattei S., Saksena S., Emr S.D. Mol. Biol. Cell 19:475-484(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH IST1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z28260 Genomic DNA. No translation available.
BK006944 Genomic DNA. Translation: DAA09188.1.

RefSeq

NP_012961.3. NM_001184309.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3GGZ	X-ray	3.80	E/F/G/H	176-204	[»]

ProteinModelPortal

P69771.

ModBase

Search...

Protein-protein interaction databases

IntAct

P69771. 4 interactions.

MINT

MINT-4493543.

STRING

4932.YKR035W-A.

Proteomic databases

PaxDb

P69771.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YKR035W-A; YKR035W-A; YKR035W-A.

GeneID

853906.

KEGG

sce:YKR035W-A.

Organism-specific databases

CYGD

YKR035w-a.

SGD

S000006435. DID2.

Phylogenomic databases

eggNOG

COG5491.

GeneTree

ENSGT00550000074957.

HOGENOM

HOG000241980.

K12197.

OrthoDB

EOG42Z80Q.

Enzyme and pathway databases

BioCyc

YEAST:G3O-32071-MONOMER.

Gene expression databases

Genevestigator

P69771.

GermOnline

YKR035W-A. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR005024. Snf7.
[Graphical view]

Pfam

PF03357. Snf7. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P69771.

NextBio

975236.

Entry information

Entry name

DID2_YEAST

Accession

Primary (citable) accession number: P69771
Secondary accession number(s): D6VX98

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	April 26, 2005
Last modified:	May 29, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents