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Protein

Vacuolar protein-sorting-associated protein 46

Gene

DID2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Class E VPS protein implicated in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins will be targeted into the vacuole after fusion of the endosome with the vacuole. Probably acts as a peripherally associated component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruits late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. Regulates the membrane association of VPS4. Can stimulate VPS4 ATPase activity directly or via VTA1.7 Publications

GO - Biological processi

  1. late endosome to vacuole transport Source: SGD
  2. late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
  3. protein targeting to vacuole Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32071-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein-sorting-associated protein 46
Alternative name(s):
Charged multivesicular body protein 1
DOA4-independent degradation protein 2
Fifty two inhibitor 1
Gene namesi
Name:DID2
Synonyms:CHM1, FTI1, VPS46
Ordered Locus Names:YKR035W-A
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKR035w-a.
SGDiS000006435. DID2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. endosome membrane Source: UniProtKB-SubCell
  3. late endosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi198 – 1981R → D: Impairs sorting. 1 Publication
Mutagenesisi199 – 1991L → D: Impairs sorting; when associated with D-202. 1 Publication
Mutagenesisi202 – 2021L → D: Impairs sorting; when associated with D-199. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 204204Vacuolar protein-sorting-associated protein 46PRO_0000211461Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei5 – 51Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP69771.
PaxDbiP69771.

Expressioni

Gene expression databases

GenevestigatoriP69771.

Interactioni

Subunit structurei

Self-associates. Interacts with VPS4 and VTA1. Interacts with IST1.6 Publications

Protein-protein interaction databases

BioGridi34166. 84 interactions.
DIPiDIP-52223N.
IntActiP69771. 4 interactions.
MINTiMINT-4493543.
STRINGi4932.YKR035W-A.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GGZX-ray3.80E/F/G/H176-204[»]
ProteinModelPortaliP69771.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69771.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 103103Interaction with VSP24Add
BLAST
Regioni104 – 204101Interaction with VSP4Add
BLAST
Regioni176 – 20429Interaction with VTA1Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili9 – 5648Sequence AnalysisAdd
BLAST
Coiled coili109 – 12921Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the SNF7 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5491.
GeneTreeiENSGT00550000074957.
HOGENOMiHOG000241980.
InParanoidiP69771.
KOiK12197.
OrthoDBiEOG77DJJM.

Family and domain databases

InterProiIPR005024. Snf7_fam.
[Graphical view]
PfamiPF03357. Snf7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69771-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRNSAAGLE NTLFQLKFTS KQLQKQANKA SKEEKQETNK LKRALNENED
60 70 80 90 100
ISRIYASNAI RKKNERLQLL KLASRVDSVA SRVQTAVTMR QVSASMGQVC
110 120 130 140 150
KGMDKALQNM NLQQITMIMD KFEQQFEDLD TSVNVYEDMG VNSDAMLVDN
160 170 180 190 200
DKVDELMSKV ADENGMELKQ SAKLDNVPEI KAKEVNVDDE KEDKLAQRLR

ALRG
Length:204
Mass (Da):23,091
Last modified:April 26, 2005 - v1
Checksum:i70D1CF4588A8C6E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28260 Genomic DNA. No translation available.
BK006944 Genomic DNA. Translation: DAA09188.1.
RefSeqiNP_012961.3. NM_001184309.3.

Genome annotation databases

EnsemblFungiiYKR035W-A; YKR035W-A; YKR035W-A.
GeneIDi853906.
KEGGisce:YKR035W-A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28260 Genomic DNA. No translation available.
BK006944 Genomic DNA. Translation: DAA09188.1.
RefSeqiNP_012961.3. NM_001184309.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GGZX-ray3.80E/F/G/H176-204[»]
ProteinModelPortaliP69771.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34166. 84 interactions.
DIPiDIP-52223N.
IntActiP69771. 4 interactions.
MINTiMINT-4493543.
STRINGi4932.YKR035W-A.

Proteomic databases

MaxQBiP69771.
PaxDbiP69771.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKR035W-A; YKR035W-A; YKR035W-A.
GeneIDi853906.
KEGGisce:YKR035W-A.

Organism-specific databases

CYGDiYKR035w-a.
SGDiS000006435. DID2.

Phylogenomic databases

eggNOGiCOG5491.
GeneTreeiENSGT00550000074957.
HOGENOMiHOG000241980.
InParanoidiP69771.
KOiK12197.
OrthoDBiEOG77DJJM.

Enzyme and pathway databases

BioCyciYEAST:G3O-32071-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP69771.
NextBioi975236.

Gene expression databases

GenevestigatoriP69771.

Family and domain databases

InterProiIPR005024. Snf7_fam.
[Graphical view]
PfamiPF03357. Snf7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways."
    Amerik A.Y., Nowak J., Swaminathan S., Hochstrasser M.
    Mol. Biol. Cell 11:3365-3380(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  4. "CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins."
    Howard T.L., Stauffer D.R., Degnin C.R., Hollenberg S.M.
    J. Cell Sci. 114:2395-2404(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae."
    Bowers K., Lottridge J., Helliwell S.B., Goldthwaite L.M., Luzio J.P., Stevens T.H.
    Traffic 5:194-210(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SELF-ASSOCIATION, INTERACTION WITH VPS4 AND VTA1.
  8. "Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes."
    Nickerson D.P., West M., Odorizzi G.
    J. Cell Biol. 175:715-720(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH VPS4 AND VPS24.
  9. Erratum
    Nickerson D.P., West M., Odorizzi G.
    J. Cell Biol. 175:1043-1043(2006)
  10. "Vta1p and Vps46p regulate the membrane association and ATPase activity of Vps4p at the yeast multivesicular body."
    Lottridge J.M., Flannery A.R., Vincelli J.L., Stevens T.H.
    Proc. Natl. Acad. Sci. U.S.A. 103:6202-6207(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH VSP4 AND VTA1.
  11. "Structural basis for selective recognition of ESCRT-III by the AAA ATPase Vps4."
    Obita T., Saksena S., Ghazi-Tabatabai S., Gill D.J., Perisic O., Emr S.D., Williams R.L.
    Nature 449:735-739(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VSP4, MUTAGENESIS OF ARG-198; LEU-199 AND LEU-202.
  12. "ESCRT-III family members stimulate Vps4 ATPase activity directly or via Vta1."
    Azmi I.F., Davies B.A., Xiao J., Babst M., Xu Z., Katzmann D.J.
    Dev. Cell 14:50-61(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH VTA1.
  13. "Novel Ist1-Did2 complex functions at a late step in multivesicular body sorting."
    Rue S.M., Mattei S., Saksena S., Emr S.D.
    Mol. Biol. Cell 19:475-484(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH IST1.
  14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDID2_YEAST
AccessioniPrimary (citable) accession number: P69771
Secondary accession number(s): D6VX98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: January 7, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2440 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.