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P69771

- DID2_YEAST

UniProt

P69771 - DID2_YEAST

Protein

Vacuolar protein-sorting-associated protein 46

Gene

DID2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (26 Apr 2005)
      Previous versions | rss
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    Functioni

    Class E VPS protein implicated in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins will be targeted into the vacuole after fusion of the endosome with the vacuole. Probably acts as a peripherally associated component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruits late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. Regulates the membrane association of VPS4. Can stimulate VPS4 ATPase activity directly or via VTA1.7 Publications

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. late endosome to vacuole transport Source: SGD
    2. late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
    3. protein targeting to vacuole Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32071-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar protein-sorting-associated protein 46
    Alternative name(s):
    Charged multivesicular body protein 1
    DOA4-independent degradation protein 2
    Fifty two inhibitor 1
    Gene namesi
    Name:DID2
    Synonyms:CHM1, FTI1, VPS46
    Ordered Locus Names:YKR035W-A
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKR035w-a.
    SGDiS000006435. DID2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. endosome membrane Source: UniProtKB-SubCell
    3. late endosome Source: SGD

    Keywords - Cellular componenti

    Endosome, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi198 – 1981R → D: Impairs sorting. 1 Publication
    Mutagenesisi199 – 1991L → D: Impairs sorting; when associated with D-202. 1 Publication
    Mutagenesisi202 – 2021L → D: Impairs sorting; when associated with D-199. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 204204Vacuolar protein-sorting-associated protein 46PRO_0000211461Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei5 – 51Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP69771.
    PaxDbiP69771.

    Expressioni

    Gene expression databases

    GenevestigatoriP69771.

    Interactioni

    Subunit structurei

    Self-associates. Interacts with VPS4 and VTA1. Interacts with IST1.6 Publications

    Protein-protein interaction databases

    BioGridi34166. 81 interactions.
    DIPiDIP-52223N.
    IntActiP69771. 4 interactions.
    MINTiMINT-4493543.
    STRINGi4932.YKR035W-A.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3GGZX-ray3.80E/F/G/H176-204[»]
    ProteinModelPortaliP69771.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP69771.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 103103Interaction with VSP24Add
    BLAST
    Regioni104 – 204101Interaction with VSP4Add
    BLAST
    Regioni176 – 20429Interaction with VTA1Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili9 – 5648Sequence AnalysisAdd
    BLAST
    Coiled coili109 – 12921Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SNF7 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5491.
    GeneTreeiENSGT00550000074957.
    HOGENOMiHOG000241980.
    KOiK12197.
    OrthoDBiEOG77DJJM.

    Family and domain databases

    InterProiIPR005024. Snf7.
    [Graphical view]
    PfamiPF03357. Snf7. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P69771-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRNSAAGLE NTLFQLKFTS KQLQKQANKA SKEEKQETNK LKRALNENED    50
    ISRIYASNAI RKKNERLQLL KLASRVDSVA SRVQTAVTMR QVSASMGQVC 100
    KGMDKALQNM NLQQITMIMD KFEQQFEDLD TSVNVYEDMG VNSDAMLVDN 150
    DKVDELMSKV ADENGMELKQ SAKLDNVPEI KAKEVNVDDE KEDKLAQRLR 200
    ALRG 204
    Length:204
    Mass (Da):23,091
    Last modified:April 26, 2005 - v1
    Checksum:i70D1CF4588A8C6E6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28260 Genomic DNA. No translation available.
    BK006944 Genomic DNA. Translation: DAA09188.1.
    RefSeqiNP_012961.3. NM_001184309.3.

    Genome annotation databases

    EnsemblFungiiYKR035W-A; YKR035W-A; YKR035W-A.
    GeneIDi853906.
    KEGGisce:YKR035W-A.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28260 Genomic DNA. No translation available.
    BK006944 Genomic DNA. Translation: DAA09188.1 .
    RefSeqi NP_012961.3. NM_001184309.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3GGZ X-ray 3.80 E/F/G/H 176-204 [» ]
    ProteinModelPortali P69771.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34166. 81 interactions.
    DIPi DIP-52223N.
    IntActi P69771. 4 interactions.
    MINTi MINT-4493543.
    STRINGi 4932.YKR035W-A.

    Proteomic databases

    MaxQBi P69771.
    PaxDbi P69771.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKR035W-A ; YKR035W-A ; YKR035W-A .
    GeneIDi 853906.
    KEGGi sce:YKR035W-A.

    Organism-specific databases

    CYGDi YKR035w-a.
    SGDi S000006435. DID2.

    Phylogenomic databases

    eggNOGi COG5491.
    GeneTreei ENSGT00550000074957.
    HOGENOMi HOG000241980.
    KOi K12197.
    OrthoDBi EOG77DJJM.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32071-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P69771.
    NextBioi 975236.

    Gene expression databases

    Genevestigatori P69771.

    Family and domain databases

    InterProi IPR005024. Snf7.
    [Graphical view ]
    Pfami PF03357. Snf7. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways."
      Amerik A.Y., Nowak J., Swaminathan S., Hochstrasser M.
      Mol. Biol. Cell 11:3365-3380(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    4. "CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins."
      Howard T.L., Stauffer D.R., Degnin C.R., Hollenberg S.M.
      J. Cell Sci. 114:2395-2404(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae."
      Bowers K., Lottridge J., Helliwell S.B., Goldthwaite L.M., Luzio J.P., Stevens T.H.
      Traffic 5:194-210(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SELF-ASSOCIATION, INTERACTION WITH VPS4 AND VTA1.
    8. "Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes."
      Nickerson D.P., West M., Odorizzi G.
      J. Cell Biol. 175:715-720(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH VPS4 AND VPS24.
    9. Erratum
      Nickerson D.P., West M., Odorizzi G.
      J. Cell Biol. 175:1043-1043(2006)
    10. "Vta1p and Vps46p regulate the membrane association and ATPase activity of Vps4p at the yeast multivesicular body."
      Lottridge J.M., Flannery A.R., Vincelli J.L., Stevens T.H.
      Proc. Natl. Acad. Sci. U.S.A. 103:6202-6207(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH VSP4 AND VTA1.
    11. "Structural basis for selective recognition of ESCRT-III by the AAA ATPase Vps4."
      Obita T., Saksena S., Ghazi-Tabatabai S., Gill D.J., Perisic O., Emr S.D., Williams R.L.
      Nature 449:735-739(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH VSP4, MUTAGENESIS OF ARG-198; LEU-199 AND LEU-202.
    12. "ESCRT-III family members stimulate Vps4 ATPase activity directly or via Vta1."
      Azmi I.F., Davies B.A., Xiao J., Babst M., Xu Z., Katzmann D.J.
      Dev. Cell 14:50-61(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH VTA1.
    13. "Novel Ist1-Did2 complex functions at a late step in multivesicular body sorting."
      Rue S.M., Mattei S., Saksena S., Emr S.D.
      Mol. Biol. Cell 19:475-484(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH IST1.
    14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDID2_YEAST
    AccessioniPrimary (citable) accession number: P69771
    Secondary accession number(s): D6VX98
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2440 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3