Reviewed, UniProtKB/Swiss-Prot P69771 (DID2_YEAST)

Names and origin

Protein names

Recommended name:
    Vacuolar protein-sorting-associated protein 46
Alternative name(s):
    Charged multivesicular body protein 1
    DOA4-independent degradation protein 2
    Fifty two inhibitor 1

Gene names

Name:	DID2
Synonyms:	CHM1, FTI1, VPS46
Ordered Locus Names:	YKR035W-A

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Class E VPS protein implicated in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins will be targeted into the vacuole after fusion of the endosome with the vacuole. Probably acts as a peripherally asocciated component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruits late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. Regulates the membrane association of VPS4. Can stimulate VPS4 ATPase activity directly or via VTA1. Ref.2 Ref.3 Ref.6 Ref.7 Ref.9 Ref.11 Ref.12
Subunit structure	Self-associates. Interacts with VPS4 and VTA1. Interacts with IST1. Ref.6 Ref.7 Ref.9 Ref.11 Ref.12 Ref.10
Subcellular location	Endosome membrane; Peripheral membrane protein. Endomembrane system; Peripheral membrane protein. Note: Endosomal and other punctate structures. Ref.2 Ref.4
Miscellaneous	Present with 2440 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the SNF7 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Endosome Membrane
Domain	Coiled coil
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	late endosome to vacuole transport via multivesicular body sorting pathway Ref.7 Inferred from mutant phenotype. Source: SGD protein targeting to vacuole Ref.2 Inferred from mutant phenotype. Source: SGD
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell late endosome Ref.2 Inferred from direct assay. Source: SGD
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-2053489,EBI-2053489
DID4	P36108	1	EBI-2053489,EBI-26574
IST1	P53843	1	EBI-2053489,EBI-28245
SNF7	P39929	1	EBI-2053489,EBI-17554
VPS24	P36095	1	EBI-2053489,EBI-26653

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 204	204	Vacuolar protein-sorting-associated protein 46		PRO_0000211461
Regions
	Region	1 – 103	103	Interaction with VSP24
	Region	104 – 204	101	Interaction with VSP4
	Region	176 – 204	29	Interaction with VTA1
	Coiled coil	9 – 56	48	Potential
	Coiled coil	109 – 129	21	Potential
Experimental info
	Mutagenesis	198	1	R → D: Impairs sorting. Ref.10
	Mutagenesis	199	1	L → D: Impairs sorting; when associated with D-202. Ref.10
	Mutagenesis	202	1	L → D: Impairs sorting; when associated with D-199. Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P69771-1 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 70D1CF4588A8C6E6	FASTA	204	23,091
	10 20 30 40 50 60 MSRNSAAGLE NTLFQLKFTS KQLQKQANKA SKEEKQETNK LKRALNENED ISRIYASNAI 70 80 90 100 110 120 RKKNERLQLL KLASRVDSVA SRVQTAVTMR QVSASMGQVC KGMDKALQNM NLQQITMIMD 130 140 150 160 170 180 KFEQQFEDLD TSVNVYEDMG VNSDAMLVDN DKVDELMSKV ADENGMELKQ SAKLDNVPEI 190 200 KAKEVNVDDE KEDKLAQRLR ALRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways." Amerik A.Y., Nowak J., Swaminathan S., Hochstrasser M. Mol. Biol. Cell 11:3365-3380(2000) [PubMed: 11029042] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[3]	"CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins." Howard T.L., Stauffer D.R., Degnin C.R., Hollenberg S.M. J. Cell Sci. 114:2395-2404(2001) [PubMed: 11559748] [Abstract] Cited for: FUNCTION.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae." Bowers K., Lottridge J., Helliwell S.B., Goldthwaite L.M., Luzio J.P., Stevens T.H. Traffic 5:194-210(2004) [PubMed: 15086794] [Abstract] Cited for: FUNCTION, SELF-ASSOCIATION, INTERACTION WITH VPS4 AND VTA1.
[7]	"Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes." Nickerson D.P., West M., Odorizzi G. J. Cell Biol. 175:715-720(2006) [PubMed: 17130288] [Abstract] Cited for: FUNCTION, INTERACTION WITH VPS4 AND VPS24.
[8]	Erratum Nickerson D.P., West M., Odorizzi G. J. Cell Biol. 175:1043-1043(2006)
[9]	"Vta1p and Vps46p regulate the membrane association and ATPase activity of Vps4p at the yeast multivesicular body." Lottridge J.M., Flannery A.R., Vincelli J.L., Stevens T.H. Proc. Natl. Acad. Sci. U.S.A. 103:6202-6207(2006) [PubMed: 16601096] [Abstract] Cited for: FUNCTION, INTERACTION WITH VSP4 AND VTA1.
[10]	"Structural basis for selective recognition of ESCRT-III by the AAA ATPase Vps4." Obita T., Saksena S., Ghazi-Tabatabai S., Gill D.J., Perisic O., Emr S.D., Williams R.L. Nature 449:735-739(2007) [PubMed: 17928861] [Abstract] Cited for: INTERACTION WITH VSP4, MUTAGENESIS OF ARG-198; LEU-199 AND LEU-202.
[11]	"ESCRT-III family members stimulate Vps4 ATPase activity directly or via Vta1." Azmi I.F., Davies B.A., Xiao J., Babst M., Xu Z., Katzmann D.J. Dev. Cell 14:50-61(2008) [PubMed: 18194652] [Abstract] Cited for: FUNCTION, INTERACTION WITH VTA1.
[12]	"Novel Ist1-Did2 complex functions at a late step in multivesicular body sorting." Rue S.M., Mattei S., Saksena S., Emr S.D. Mol. Biol. Cell 19:475-484(2008) [PubMed: 18032584] [Abstract] Cited for: FUNCTION, INTERACTION WITH IST1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Z28260 Genomic DNA. No translation available.

RefSeq

NP_012961.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3GGZ	X-ray	3.80	E/F/G/H	176-204	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P69771. 5 interactions.

STRING

P69771.

Genome annotation databases

Ensembl

YKR035W-A; YKR035W-A; YKR035W-A; Saccharomyces cerevisiae. [Genome view]

GeneID

853906.

KEGG

sce:YKR035W-A.

NMPDR

fig|4932.3.peg.3947.

Organism-specific databases

CYGD

YKR035w-a.

SGD

S000006435. DID2.

Phylogenomic databases

HOGENOM

HBG379179.

OMA

MDLQKVS.

OrthoDB

EOG91ZGT6.

Gene expression databases

ArrayExpress

P69771.

Genevestigator

P69771.

GermOnline

YKR035W-A. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR005024. Snf7.
[Graphical view]

Pfam

PF03357. Snf7. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

975236.

Entry information

Entry name

DID2_YEAST

Accession

Primary (citable) accession number: P69771

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	April 26, 2005
Last modified:	December 15, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)