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Protein

Vacuolar protein-sorting-associated protein 46

Gene

DID2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Class E VPS protein implicated in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins will be targeted into the vacuole after fusion of the endosome with the vacuole. Probably acts as a peripherally associated component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruits late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. Regulates the membrane association of VPS4. Can stimulate VPS4 ATPase activity directly or via VTA1.7 Publications

GO - Biological processi

  • late endosome to vacuole transport Source: SGD
  • late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
  • protein targeting to vacuole Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32071-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein-sorting-associated protein 46
Alternative name(s):
Charged multivesicular body protein 1
DOA4-independent degradation protein 2
Fifty two inhibitor 1
Gene namesi
Name:DID2
Synonyms:CHM1, FTI1, VPS46
Ordered Locus Names:YKR035W-A
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR035W-A.
SGDiS000006435. DID2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • endosome membrane Source: UniProtKB-SubCell
  • late endosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi198R → D: Impairs sorting. 1 Publication1
Mutagenesisi199L → D: Impairs sorting; when associated with D-202. 1 Publication1
Mutagenesisi202L → D: Impairs sorting; when associated with D-199. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002114611 – 204Vacuolar protein-sorting-associated protein 46Add BLAST204

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP69771.
PRIDEiP69771.

PTM databases

iPTMnetiP69771.

Interactioni

Subunit structurei

Self-associates. Interacts with VPS4 and VTA1. Interacts with IST1.6 Publications

Protein-protein interaction databases

BioGridi34166. 98 interactors.
DIPiDIP-52223N.
IntActiP69771. 4 interactors.
MINTiMINT-4493543.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GGZX-ray3.80E/F/G/H176-204[»]
ProteinModelPortaliP69771.
SMRiP69771.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69771.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 103Interaction with VSP24Add BLAST103
Regioni104 – 204Interaction with VSP4Add BLAST101
Regioni176 – 204Interaction with VTA1Add BLAST29

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili9 – 56Sequence analysisAdd BLAST48
Coiled coili109 – 129Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the SNF7 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00550000074957.
HOGENOMiHOG000241980.
InParanoidiP69771.
KOiK12197.
OrthoDBiEOG092C5BJC.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
InterProiIPR006674. HD_domain.
IPR005024. Snf7_fam.
[Graphical view]
PfamiPF03357. Snf7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69771-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRNSAAGLE NTLFQLKFTS KQLQKQANKA SKEEKQETNK LKRALNENED
60 70 80 90 100
ISRIYASNAI RKKNERLQLL KLASRVDSVA SRVQTAVTMR QVSASMGQVC
110 120 130 140 150
KGMDKALQNM NLQQITMIMD KFEQQFEDLD TSVNVYEDMG VNSDAMLVDN
160 170 180 190 200
DKVDELMSKV ADENGMELKQ SAKLDNVPEI KAKEVNVDDE KEDKLAQRLR

ALRG
Length:204
Mass (Da):23,091
Last modified:April 26, 2005 - v1
Checksum:i70D1CF4588A8C6E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28260 Genomic DNA. No translation available.
BK006944 Genomic DNA. Translation: DAA09188.1.
RefSeqiNP_012961.3. NM_001184309.3.

Genome annotation databases

EnsemblFungiiYKR035W-A; YKR035W-A; YKR035W-A.
GeneIDi853906.
KEGGisce:YKR035W-A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28260 Genomic DNA. No translation available.
BK006944 Genomic DNA. Translation: DAA09188.1.
RefSeqiNP_012961.3. NM_001184309.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GGZX-ray3.80E/F/G/H176-204[»]
ProteinModelPortaliP69771.
SMRiP69771.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34166. 98 interactors.
DIPiDIP-52223N.
IntActiP69771. 4 interactors.
MINTiMINT-4493543.

PTM databases

iPTMnetiP69771.

Proteomic databases

MaxQBiP69771.
PRIDEiP69771.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKR035W-A; YKR035W-A; YKR035W-A.
GeneIDi853906.
KEGGisce:YKR035W-A.

Organism-specific databases

EuPathDBiFungiDB:YKR035W-A.
SGDiS000006435. DID2.

Phylogenomic databases

GeneTreeiENSGT00550000074957.
HOGENOMiHOG000241980.
InParanoidiP69771.
KOiK12197.
OrthoDBiEOG092C5BJC.

Enzyme and pathway databases

BioCyciYEAST:G3O-32071-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP69771.
PROiP69771.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
InterProiIPR006674. HD_domain.
IPR005024. Snf7_fam.
[Graphical view]
PfamiPF03357. Snf7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDID2_YEAST
AccessioniPrimary (citable) accession number: P69771
Secondary accession number(s): D6VX98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: November 30, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2440 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.