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Protein

Transient receptor potential cation channel subfamily V member 5

Gene

Trpv5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Constitutively active calcium selective cation channel thought to be involved in Ca2+ reabsorption in kidney and intestine. The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca2+-dependent feedback regulation includes fast channel inactivation and slow current decay. Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity).By similarity1 Publication

Enzyme regulationi

Activated by WNK3.By similarity

GO - Molecular functioni

  • calcium channel activity Source: UniProtKB

GO - Biological processi

  • calcium ion transport Source: UniProtKB
  • calcium ion transport into cytosol Source: Ensembl
  • cellular calcium ion homeostasis Source: UniProtKB
  • protein tetramerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Calmodulin-binding

Enzyme and pathway databases

ReactomeiR-MMU-3295583. TRP channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Transient receptor potential cation channel subfamily V member 5
Short name:
TrpV5
Alternative name(s):
Calcium transport protein 2
Short name:
CaT2
Epithelial calcium channel 1
Short name:
ECaC1
Osm-9-like TRP channel 3
Short name:
OTRPC3
Gene namesi
Name:Trpv5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2429764. Trpv5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 320320CytoplasmicSequence analysisAdd
BLAST
Transmembranei321 – 34121HelicalSequence analysisAdd
BLAST
Topological domaini342 – 38039ExtracellularSequence analysisAdd
BLAST
Transmembranei381 – 40121HelicalSequence analysisAdd
BLAST
Topological domaini402 – 41211ExtracellularSequence analysisAdd
BLAST
Transmembranei413 – 43321HelicalSequence analysisAdd
BLAST
Topological domaini434 – 4418CytoplasmicSequence analysis
Transmembranei442 – 46221HelicalSequence analysisAdd
BLAST
Topological domaini463 – 48523ExtracellularSequence analysisAdd
BLAST
Transmembranei486 – 50621HelicalSequence analysisAdd
BLAST
Intramembranei517 – 53721Pore-formingCuratedAdd
BLAST
Transmembranei550 – 57021HelicalSequence analysisAdd
BLAST
Topological domaini571 – 723153CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: MGI
  • integral component of plasma membrane Source: UniProtKB
  • intracellular Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi403 – 4064VGAS → LGVT: Induces faster Ca(2+)-dependent channel inactivation. 1 Publication
Mutagenesisi593 – 5931T → A: Abolishes interaction with S100A10, plasma membrane localization and channel activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 723723Transient receptor potential cation channel subfamily V member 5PRO_0000215351Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei678 – 6781PhosphothreonineCombined sources
Modified residuei682 – 6821PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP69744.
PRIDEiP69744.

PTM databases

iPTMnetiP69744.
PhosphoSiteiP69744.

Expressioni

Gene expression databases

BgeeiENSMUSG00000036899.
ExpressionAtlasiP69744. baseline and differential.

Interactioni

Subunit structurei

Homotetramer and probably heterotetramer with TRPV6. Interacts with TRPV6 (By similarity). Interacts with S100A10 and probably with the ANAX2-S100A10 heterotetramer. The interaction with S100A10 is required for the trafficking to the plasma membrane. Interacts with calmodulin. Interacts with BSPRY, which results in its inactivation.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Wnk4Q80UE62EBI-538447,EBI-295378

Protein-protein interaction databases

BioGridi228781. 1 interaction.
IntActiP69744. 2 interactions.
STRINGi10090.ENSMUSP00000031901.

Structurei

3D structure databases

ProteinModelPortaliP69744.
SMRiP69744. Positions 38-606.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati72 – 10130ANK 1Add
BLAST
Repeati110 – 13930ANK 2Add
BLAST
Repeati156 – 18530ANK 3Add
BLAST
Repeati232 – 26130ANK 4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni591 – 5955Interaction with S100A10
Regioni643 – 6464Involved in Ca(2+)-dependent inactivationBy similarity
Regioni693 – 72331Involved in Ca(2+)-dependent inactivationBy similarityAdd
BLAST

Sequence similaritiesi

Contains 4 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3676. Eukaryota.
ENOG4110DG4. LUCA.
GeneTreeiENSGT00550000074425.
HOGENOMiHOG000234397.
HOVERGENiHBG061442.
InParanoidiP69744.
KOiK04974.
OMAiVNLPFMF.
OrthoDBiEOG091G0314.
TreeFamiTF314711.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR008346. TRPV5.
IPR008344. TRPV5/TRPV6.
[Graphical view]
PANTHERiPTHR10582:SF11. PTHR10582:SF11. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
PR01765. ECACCHANNEL.
PR01767. ECACCHANNEL2.
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69744-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAKTPWIQL QKLLNWWVRD QDWNQHVDQL HMLQQKSIWE SPLLRAAKEN
60 70 80 90 100
DMCTLKKLQH DQNCDFRQRG ALGETALHVA ALYDNLDAAI MLMEAAPYLV
110 120 130 140 150
TESTLCEPFV GQTALHIAVM NQNVNLVRAL LARGASASAR ATGSAFHRSS
160 170 180 190 200
HNLIYYGEHP LSFAACVGSE EIVRLLIEHG ADIRAQDSLG NTVLHILVLQ
210 220 230 240 250
PNKTFACQMY NLLLSYDGGD HLKSLELVPN NQGLTPFKLA GVEGNTVMFQ
260 270 280 290 300
HLMQKRKRIQ WSFGPLTSSL YDLTEIDSWG EELSFLELVV SSKKKEARQI
310 320 330 340 350
LEQTPVKELV SLKWKKYGQP YFCLLGALYI FYMVCFTTCC VYRPLKFRDA
360 370 380 390 400
NRTHVRDNTI MEQKSLQEAY VTYQDKIRLV GELVTVIGAV IILLLEIPDI
410 420 430 440 450
FRVGASRYFG QTVLGGPFHV IIITYASLVL LTMAMRLTNV NGEVVPMSMA
460 470 480 490 500
LVLGWCSVMY FARGFQMLGP FTIMIQKMIF GDLLRFCWLM AMVILGFASA
510 520 530 540 550
FYIIFQTEDP DNLGEFSDYP TAMFSTFELF LTIIDGPANY RVDLPFMYSV
560 570 580 590 600
TYATFAIIAT LLMLNLFIAM MGDTHWRVAQ ERDELWRAQV VATTVMLERK
610 620 630 640 650
MPRFLWPRSG ICGCEYGLGD RWFLRVEHHQ EQNPYRVLRY VEAFKSSDKE
660 670 680 690 700
EVQEQLSEKQ PSGTETGTLA RGSVVLQTPP LSRTTSLSSN SHRGWEILRR
710 720
NTLGHLNLGL DPGEGDGEEI YQF
Length:723
Mass (Da):82,218
Last modified:July 27, 2011 - v2
Checksum:i6668C67C47CBB443
GO

Sequence cautioni

The sequence AK085479 differs from that shown. Reason: Frameshift at position 579. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti302 – 3021E → K in AK085479 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF336378 Genomic DNA. Translation: AAM53408.1.
AK085479 mRNA. No translation available.
BC110554 mRNA. Translation: AAI10555.1.
BC110555 mRNA. Translation: AAI10556.1.
CH466533 Genomic DNA. Translation: EDL13503.1.
CCDSiCCDS20053.1.
RefSeqiNP_001007573.1. NM_001007572.2.
XP_006505831.1. XM_006505768.2.
XP_011239559.1. XM_011241257.1.
UniGeneiMm.135734.

Genome annotation databases

EnsembliENSMUST00000031901; ENSMUSP00000031901; ENSMUSG00000036899.
GeneIDi194352.
KEGGimmu:194352.
UCSCiuc009bqd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF336378 Genomic DNA. Translation: AAM53408.1.
AK085479 mRNA. No translation available.
BC110554 mRNA. Translation: AAI10555.1.
BC110555 mRNA. Translation: AAI10556.1.
CH466533 Genomic DNA. Translation: EDL13503.1.
CCDSiCCDS20053.1.
RefSeqiNP_001007573.1. NM_001007572.2.
XP_006505831.1. XM_006505768.2.
XP_011239559.1. XM_011241257.1.
UniGeneiMm.135734.

3D structure databases

ProteinModelPortaliP69744.
SMRiP69744. Positions 38-606.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228781. 1 interaction.
IntActiP69744. 2 interactions.
STRINGi10090.ENSMUSP00000031901.

PTM databases

iPTMnetiP69744.
PhosphoSiteiP69744.

Proteomic databases

PaxDbiP69744.
PRIDEiP69744.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031901; ENSMUSP00000031901; ENSMUSG00000036899.
GeneIDi194352.
KEGGimmu:194352.
UCSCiuc009bqd.1. mouse.

Organism-specific databases

CTDi56302.
MGIiMGI:2429764. Trpv5.

Phylogenomic databases

eggNOGiKOG3676. Eukaryota.
ENOG4110DG4. LUCA.
GeneTreeiENSGT00550000074425.
HOGENOMiHOG000234397.
HOVERGENiHBG061442.
InParanoidiP69744.
KOiK04974.
OMAiVNLPFMF.
OrthoDBiEOG091G0314.
TreeFamiTF314711.

Enzyme and pathway databases

ReactomeiR-MMU-3295583. TRP channels.

Miscellaneous databases

PROiP69744.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036899.
ExpressionAtlasiP69744. baseline and differential.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR008346. TRPV5.
IPR008344. TRPV5/TRPV6.
[Graphical view]
PANTHERiPTHR10582:SF11. PTHR10582:SF11. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
PR01765. ECACCHANNEL.
PR01767. ECACCHANNEL2.
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPV5_MOUSE
AccessioniPrimary (citable) accession number: P69744
Secondary accession number(s): Q2TB50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.