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Protein

Transient receptor potential cation channel subfamily V member 5

Gene

Trpv5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Constitutively active calcium selective cation channel thought to be involved in Ca2+ reabsorption in kidney and intestine (PubMed:12077127, PubMed:14679186). Required for normal Ca2+ reabsorption in the kidney distal convoluted tubules (PubMed:14679186). The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca2+-dependent feedback regulation includes fast channel inactivation and slow current decay (PubMed:12077127). Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity).By similarity2 Publications

Enzyme regulationi

Activated by WNK3.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi535Calcium; shared with neighboring subunitsBy similarity1

GO - Molecular functioni

  • calcium channel activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • calcium ion homeostasis Source: UniProtKB
  • calcium ion import into cell Source: UniProtKB
  • calcium ion transmembrane transport Source: MGI
  • calcium ion transport Source: UniProtKB
  • calcium ion transport into cytosol Source: Ensembl
  • cellular calcium ion homeostasis Source: UniProtKB
  • protein tetramerization Source: UniProtKB
  • regulation of urine volume Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Calmodulin-binding, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-3295583. TRP channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Transient receptor potential cation channel subfamily V member 5
Short name:
TrpV5
Alternative name(s):
Calcium transport protein 2
Short name:
CaT2
Epithelial calcium channel 1
Short name:
ECaC1
Osm-9-like TRP channel 3
Short name:
OTRPC3
Gene namesi
Name:Trpv5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2429764. Trpv5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 320CytoplasmicSequence analysisAdd BLAST320
Transmembranei321 – 341HelicalSequence analysisAdd BLAST21
Topological domaini342 – 380ExtracellularSequence analysisAdd BLAST39
Transmembranei381 – 401HelicalSequence analysisAdd BLAST21
Topological domaini402 – 412ExtracellularSequence analysisAdd BLAST11
Transmembranei413 – 433HelicalSequence analysisAdd BLAST21
Topological domaini434 – 441CytoplasmicSequence analysis8
Transmembranei442 – 462HelicalSequence analysisAdd BLAST21
Topological domaini463 – 485ExtracellularSequence analysisAdd BLAST23
Transmembranei486 – 506HelicalSequence analysisAdd BLAST21
Intramembranei517 – 537Pore-formingCuratedAdd BLAST21
Transmembranei550 – 570HelicalSequence analysisAdd BLAST21
Topological domaini571 – 723CytoplasmicSequence analysisAdd BLAST153

GO - Cellular componenti

  • apical plasma membrane Source: MGI
  • integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant mice appear grossly normal and are fertile. They display polyuria and increased urinary excretion of Ca2+, due to defective Ca2+ reabsorption in the kidney distal convoluted tubules. Likewise, they display increased urinary excretion of phosphate. Besides, their urine has a lower pH. The polyuria and the urine acidification may be a response to the high urinary Ca2+ levels, preventing the formation of kidney stones (PubMed:14679186). Serum Ca2+ and phosphate levels are normal, probably due to increased expression of TRPV6 and increased Ca2+ absorption in the intestine. The increased expression ot TRPV6 may be due to the increased serum levels of 1,25-dihydroxy-vitamin D3 that are observed in mutant mice (PubMed:14679186, PubMed:27102152). Age-related changes in trabecular and cortical bone mass are accelerated in male mutant mice, including reduced trabecular and cortical bone thickness. Still, this has no effect on bone strength (PubMed:27102152).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi403 – 406VGAS → LGVT: Induces faster Ca(2+)-dependent channel inactivation. 1 Publication4
Mutagenesisi593T → A: Abolishes interaction with S100A10, plasma membrane localization and channel activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002153511 – 723Transient receptor potential cation channel subfamily V member 5Add BLAST723

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei678PhosphothreonineCombined sources1
Modified residuei682PhosphoserineCombined sources1

Post-translational modificationi

Glycosylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP69744.
PRIDEiP69744.

PTM databases

iPTMnetiP69744.
PhosphoSitePlusiP69744.

Expressioni

Tissue specificityi

Detected in kidney cortex (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000036899.
ExpressionAtlasiP69744. baseline and differential.

Interactioni

Subunit structurei

Homotetramer and probably heterotetramer with TRPV6. Interacts with TRPV6 (PubMed:12574114). Interacts with S100A10 and probably with the ANAX2-S100A10 heterotetramer. The interaction with S100A10 is required for the trafficking to the plasma membrane (PubMed:12660155). Interacts with calmodulin (PubMed:15123711). Interacts with BSPRY, which results in its inactivation (PubMed:16380433).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Wnk4Q80UE62EBI-538447,EBI-295378

Protein-protein interaction databases

BioGridi228781. 1 interactor.
IntActiP69744. 2 interactors.
STRINGi10090.ENSMUSP00000031901.

Structurei

3D structure databases

ProteinModelPortaliP69744.
SMRiP69744.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati38 – 68ANK 1Sequence analysisAdd BLAST31
Repeati72 – 101ANK 2Sequence analysisAdd BLAST30
Repeati110 – 139ANK 3Sequence analysisAdd BLAST30
Repeati156 – 185ANK 4Sequence analysisAdd BLAST30
Repeati189 – 222ANK 5Sequence analysisAdd BLAST34
Repeati232 – 261ANK 6Sequence analysisAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni591 – 595Interaction with S100A101 Publication5
Regioni643 – 646Involved in Ca(2+)-dependent inactivationBy similarity4
Regioni693 – 723Involved in Ca(2+)-dependent inactivationBy similarityAdd BLAST31

Sequence similaritiesi

Contains 6 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3676. Eukaryota.
ENOG4110DG4. LUCA.
GeneTreeiENSGT00550000074425.
HOGENOMiHOG000234397.
HOVERGENiHBG061442.
InParanoidiP69744.
KOiK04974.
OMAiVNLPFMF.
OrthoDBiEOG091G0314.
TreeFamiTF314711.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR008346. TRPV5.
IPR008344. TRPV5/TRPV6.
[Graphical view]
PANTHERiPTHR10582:SF11. PTHR10582:SF11. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
PR01765. ECACCHANNEL.
PR01767. ECACCHANNEL2.
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69744-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAKTPWIQL QKLLNWWVRD QDWNQHVDQL HMLQQKSIWE SPLLRAAKEN
60 70 80 90 100
DMCTLKKLQH DQNCDFRQRG ALGETALHVA ALYDNLDAAI MLMEAAPYLV
110 120 130 140 150
TESTLCEPFV GQTALHIAVM NQNVNLVRAL LARGASASAR ATGSAFHRSS
160 170 180 190 200
HNLIYYGEHP LSFAACVGSE EIVRLLIEHG ADIRAQDSLG NTVLHILVLQ
210 220 230 240 250
PNKTFACQMY NLLLSYDGGD HLKSLELVPN NQGLTPFKLA GVEGNTVMFQ
260 270 280 290 300
HLMQKRKRIQ WSFGPLTSSL YDLTEIDSWG EELSFLELVV SSKKKEARQI
310 320 330 340 350
LEQTPVKELV SLKWKKYGQP YFCLLGALYI FYMVCFTTCC VYRPLKFRDA
360 370 380 390 400
NRTHVRDNTI MEQKSLQEAY VTYQDKIRLV GELVTVIGAV IILLLEIPDI
410 420 430 440 450
FRVGASRYFG QTVLGGPFHV IIITYASLVL LTMAMRLTNV NGEVVPMSMA
460 470 480 490 500
LVLGWCSVMY FARGFQMLGP FTIMIQKMIF GDLLRFCWLM AMVILGFASA
510 520 530 540 550
FYIIFQTEDP DNLGEFSDYP TAMFSTFELF LTIIDGPANY RVDLPFMYSV
560 570 580 590 600
TYATFAIIAT LLMLNLFIAM MGDTHWRVAQ ERDELWRAQV VATTVMLERK
610 620 630 640 650
MPRFLWPRSG ICGCEYGLGD RWFLRVEHHQ EQNPYRVLRY VEAFKSSDKE
660 670 680 690 700
EVQEQLSEKQ PSGTETGTLA RGSVVLQTPP LSRTTSLSSN SHRGWEILRR
710 720
NTLGHLNLGL DPGEGDGEEI YQF
Length:723
Mass (Da):82,218
Last modified:July 27, 2011 - v2
Checksum:i6668C67C47CBB443
GO

Sequence cautioni

The sequence AK085479 differs from that shown. Reason: Frameshift at position 579.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti302E → K in AK085479 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF336378 Genomic DNA. Translation: AAM53408.1.
AK085479 mRNA. No translation available.
BC110554 mRNA. Translation: AAI10555.1.
BC110555 mRNA. Translation: AAI10556.1.
CH466533 Genomic DNA. Translation: EDL13503.1.
CCDSiCCDS20053.1.
RefSeqiNP_001007573.1. NM_001007572.2.
XP_017176952.1. XM_017321463.1.
UniGeneiMm.135734.

Genome annotation databases

EnsembliENSMUST00000031901; ENSMUSP00000031901; ENSMUSG00000036899.
GeneIDi194352.
KEGGimmu:194352.
UCSCiuc009bqd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF336378 Genomic DNA. Translation: AAM53408.1.
AK085479 mRNA. No translation available.
BC110554 mRNA. Translation: AAI10555.1.
BC110555 mRNA. Translation: AAI10556.1.
CH466533 Genomic DNA. Translation: EDL13503.1.
CCDSiCCDS20053.1.
RefSeqiNP_001007573.1. NM_001007572.2.
XP_017176952.1. XM_017321463.1.
UniGeneiMm.135734.

3D structure databases

ProteinModelPortaliP69744.
SMRiP69744.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228781. 1 interactor.
IntActiP69744. 2 interactors.
STRINGi10090.ENSMUSP00000031901.

PTM databases

iPTMnetiP69744.
PhosphoSitePlusiP69744.

Proteomic databases

PaxDbiP69744.
PRIDEiP69744.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031901; ENSMUSP00000031901; ENSMUSG00000036899.
GeneIDi194352.
KEGGimmu:194352.
UCSCiuc009bqd.1. mouse.

Organism-specific databases

CTDi56302.
MGIiMGI:2429764. Trpv5.

Phylogenomic databases

eggNOGiKOG3676. Eukaryota.
ENOG4110DG4. LUCA.
GeneTreeiENSGT00550000074425.
HOGENOMiHOG000234397.
HOVERGENiHBG061442.
InParanoidiP69744.
KOiK04974.
OMAiVNLPFMF.
OrthoDBiEOG091G0314.
TreeFamiTF314711.

Enzyme and pathway databases

ReactomeiR-MMU-3295583. TRP channels.

Miscellaneous databases

PROiP69744.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036899.
ExpressionAtlasiP69744. baseline and differential.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR008346. TRPV5.
IPR008344. TRPV5/TRPV6.
[Graphical view]
PANTHERiPTHR10582:SF11. PTHR10582:SF11. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
PR01765. ECACCHANNEL.
PR01767. ECACCHANNEL2.
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPV5_MOUSE
AccessioniPrimary (citable) accession number: P69744
Secondary accession number(s): Q2TB50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.