P69743 (MBHT_ECO57) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 56.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Hydrogenase-2 small chain Short name=HYD2 EC=1.12.99.6 Alternative name(s): Membrane-bound hydrogenase 2 small subunit NiFe hydrogenase | ||||
| Gene names |
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| Organism | Escherichia coli O157:H7 [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 83334 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 372 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake. |
| Catalytic activity | H2 + A = AH2. |
| Cofactor | Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity. |
| Subunit structure | Heterodimer of a large and a small subunit. |
| Subcellular location | Cell membrane; Peripheral membrane protein; Periplasmic side. Periplasm. |
| Post-translational modification | Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. |
| Sequence similarities | Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 37 | 37 | Tat-type signal Potential | ||||||
| Chain | 38 – 372 | 335 | Hydrogenase-2 small chain | PRO_0000013432 | |||||
Sites | |||||||||
| Metal binding | 59 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 62 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 157 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 191 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 229 | 1 | Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity | ||||||
| Metal binding | 232 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 257 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 263 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 272 | 1 | Iron-sulfur 3 (3Fe-4S) By similarity | ||||||
| Metal binding | 292 | 1 | Iron-sulfur 3 (3Fe-4S) By similarity | ||||||
| Metal binding | 295 | 1 | Iron-sulfur 3 (3Fe-4S) By similarity | ||||||
Sequences
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References
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE005174 Genomic DNA. Translation: AAG58134.1. BA000007 Genomic DNA. Translation: BAB37305.1. |
| PIR | B85959. B91114. |
| RefSeq | NP_289575.1. NC_002655.2. NP_311909.1. NC_002695.1. |
3D structure databases | |
| ProteinModelPortal | P69743. |
| SMR | P69743. Positions 46-310. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBESCT00000024891; EBESCP00000023784; EBESCG00000023945. EBESCT00000060407; EBESCP00000058235; EBESCG00000059454. |
| GeneID | 916289. 958478. |
| GenomeReviews | Gene locus Z4351 in contig AE005174_GR. Gene locus ECs3882 in contig BA000007_GR. |
| KEGG | ece:Z4351. ecs:ECs3882. |
| PATRIC | 18357255. VBIEscCol44059_3805. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| GeneTree | EBGT00050000010145. |
| HOGENOM | HBG575615. |
| OMA | RLIHENC. |
| ProtClustDB | PRK10468. |
Enzyme and pathway databases | |
| BioCyc | ECOL83334:ECS3882-MONOMER. |
Family and domain databases | |
| InterPro | IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001821. NiFe_hydrogenase_ssu. IPR006311. TAT_signal. IPR019546. TAT_signal_bac_arc. [Graphical view] |
| Gene3D | G3DSA:3.40.50.700. G3DSA:3.40.50.700. 1 hit. |
| KO | K06282. |
| Pfam | PF01058. Oxidored_q6. 1 hit. [Graphical view] |
| PIRSF | PIRSF000310. NiFe_hyd_ssu. 1 hit. |
| PRINTS | PR00614. NIHGNASESMLL. |
| TIGRFAMs | TIGR00391. HydA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit. |
| PROSITE | PS51318. TAT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MBHT_ECO57 | ||||||||
| Accession | Primary (citable) accession number: P69743 Secondary accession number(s): Q46847 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

Clusters with