Reviewed,
UniProtKB/Swiss-Prot P69741 (MBHT_ECOLI)
Last modified
June 16, 2009.
Version 40.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Hydrogenase-2 small chain Short name=HYD2 EC=1.12.99.6 Alternative name(s): Membrane-bound hydrogenase 2 small subunit NiFe hydrogenase | ||||||
| Gene names |
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| Organism | Escherichia coli (strain K12) [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 83333 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 372 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake. |
| Catalytic activity | H2 + A = AH2. |
| Cofactor | Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity. |
| Subunit structure | Heterodimer of a large and a small subunit. |
| Subcellular location | Cell membrane; Peripheral membrane protein; Periplasmic side. |
| Post-translational modification | Exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven. |
| Sequence similarities | Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 37 | 37 | Tat-type signal Ref.3 | ||||||
| Chain | 38 – 372 | 335 | Hydrogenase-2 small chain | PRO_0000013430 | |||||
Sites | |||||||||
| Metal binding | 59 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 62 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 157 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 191 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 229 | 1 | Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity | ||||||
| Metal binding | 232 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 257 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 263 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 272 | 1 | Iron-sulfur 3 (3Fe-4S) By similarity | ||||||
| Metal binding | 292 | 1 | Iron-sulfur 3 (3Fe-4S) By similarity | ||||||
| Metal binding | 295 | 1 | Iron-sulfur 3 (3Fe-4S) By similarity | ||||||
Sequences
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References
Cross-references
Sequence databases | |
|---|---|
| U28377 Genomic DNA. Translation: AAA69164.1. U00096 Genomic DNA. Translation: AAC76033.1. AP009048 Genomic DNA. Translation: BAE77058.1. | |
| PIR | C65086. |
| RefSeq | AP_003552.1. NP_417471.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1FRF based on UniProtKB P18187. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P69741. 6 interactions. |
Genome annotation databases | |
| GeneID | 945902. |
| GenomeReviews | Gene locus JW2965 in contig AP009048_GR. Gene locus b2997 in contig U00096_GR. |
| KEGG | ecj:JW2965. eco:b2997. |
Organism-specific databases | |
| EchoBASE | EB2828. |
| EcoGene | EG13006. hybO. |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P69741. |
| OMA | P69741. RLIHENC. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:MON0-145. MetaCyc:MON0-145. |
Family and domain databases | |
| InterPro | IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001821. NiFe_hydrogenase_ssu. IPR006311. Tat. IPR017909. Twin_arg_translocation_Tat. [Graphical view] |
| Pfam | PF01058. Oxidored_q6. 1 hit. [Graphical view] |
| PIRSF | PIRSF000310. NiFe_hyd_ssu. 1 hit. |
| PRINTS | PR00614. NIHGNASESMLL. |
| TIGRFAMs | TIGR00391. hydA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit. |
| PROSITE | PS51318. TAT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MBHT_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P69741 Secondary accession number(s): Q2M9J8, Q46847 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| SIMILARITY comments Index of protein domains and families |

Clusters with


