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Protein

Hydrogenase-2 small chain

Gene

hybO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake.

Catalytic activityi

H2 + A = AH2.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi59Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi62Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi157Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi191Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi229Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity1
Metal bindingi232Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi257Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi263Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi272Iron-sulfur 3 (3Fe-4S)By similarity1
Metal bindingi292Iron-sulfur 3 (3Fe-4S)By similarity1
Metal bindingi295Iron-sulfur 3 (3Fe-4S)By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-145.
ECOL316407:JW2965-MONOMER.
MetaCyc:MONOMER0-145.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase-2 small chain (EC:1.12.99.6)
Short name:
HYD2
Alternative name(s):
Membrane-bound hydrogenase 2 small subunit
NiFe hydrogenase
Gene namesi
Name:hybO
Synonyms:yghV
Ordered Locus Names:b2997, JW2965
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13006. hybO.

Subcellular locationi

GO - Cellular componenti

  • extrinsic component of periplasmic side of plasma membrane Source: EcoCyc
  • ferredoxin hydrogenase complex Source: InterPro
  • membrane Source: UniProtKB
  • periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 37Tat-type signalPROSITE-ProRule annotation1 PublicationAdd BLAST37
ChainiPRO_000001343038 – 372Hydrogenase-2 small chainAdd BLAST335

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven. Can also be exported by the Sec system.

Proteomic databases

PaxDbiP69741.
PRIDEiP69741.

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Binary interactionsi

WithEntry#Exp.IntActNotes
hybEP0AAN14EBI-552619,EBI-552588

Protein-protein interaction databases

BioGridi4261299. 221 interactors.
DIPiDIP-36024N.
IntActiP69741. 17 interactors.
MINTiMINT-8046452.
STRINGi511145.b2997.

Structurei

3D structure databases

ProteinModelPortaliP69741.
SMRiP69741.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105E3S. Bacteria.
COG1740. LUCA.
HOGENOMiHOG000278822.
InParanoidiP69741.
KOiK06282.
OMAiRSFNAHN.
PhylomeDBiP69741.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
4.10.480.10. 1 hit.
InterProiIPR027394. Cytochrome-c3_hydrogenase_C.
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR001821. NiFe_hydrogenase_ssu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PANTHERiPTHR30013. PTHR30013. 1 hit.
PfamiPF14720. NiFe_hyd_SSU_C. 1 hit.
PF01058. Oxidored_q6. 1 hit.
[Graphical view]
PIRSFiPIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTSiPR00614. NIHGNASESMLL.
TIGRFAMsiTIGR00391. hydA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69741-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGDNTLIHS HGINRRDFMK LCAALAATMG LSSKAAAEMA ESVTNPQRPP
60 70 80 90 100
VIWIGAQECT GCTESLLRAT HPTVENLVLE TISLEYHEVL SAAFGHQVEE
110 120 130 140 150
NKHNALEKYK GQYVLVVDGS IPLKDNGIYC MVAGEPIVDH IRKAAEGAAA
160 170 180 190 200
IIAIGSCSAW GGVAAAGVNP TGAVSLQEVL PGKTVINIPG CPPNPHNFLA
210 220 230 240 250
TVAHIITYGK PPKLDDKNRP TFAYGRLIHE HCERRPHFDA GRFAKEFGDE
260 270 280 290 300
GHREGWCLYH LGCKGPETYG NCSTLQFCDV GGVWPVAIGH PCYGCNEEGI
310 320 330 340 350
GFHKGIHQLA NVENQTPRSQ KPDVNAKEGG NVSAGAIGLL GGVVGLVAGV
360 370
SVMAVRELGR QQKKDNADSR GE
Length:372
Mass (Da):39,652
Last modified:April 26, 2005 - v1
Checksum:iC5821096BBAF9C66
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28377 Genomic DNA. Translation: AAA69164.1.
U00096 Genomic DNA. Translation: AAC76033.1.
AP009048 Genomic DNA. Translation: BAE77058.1.
PIRiC65086.
RefSeqiNP_417471.1. NC_000913.3.
WP_000145410.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76033; AAC76033; b2997.
BAE77058; BAE77058; BAE77058.
GeneIDi945902.
KEGGiecj:JW2965.
eco:b2997.
PATRICi32121410. VBIEscCol129921_3092.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28377 Genomic DNA. Translation: AAA69164.1.
U00096 Genomic DNA. Translation: AAC76033.1.
AP009048 Genomic DNA. Translation: BAE77058.1.
PIRiC65086.
RefSeqiNP_417471.1. NC_000913.3.
WP_000145410.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP69741.
SMRiP69741.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261299. 221 interactors.
DIPiDIP-36024N.
IntActiP69741. 17 interactors.
MINTiMINT-8046452.
STRINGi511145.b2997.

Proteomic databases

PaxDbiP69741.
PRIDEiP69741.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76033; AAC76033; b2997.
BAE77058; BAE77058; BAE77058.
GeneIDi945902.
KEGGiecj:JW2965.
eco:b2997.
PATRICi32121410. VBIEscCol129921_3092.

Organism-specific databases

EchoBASEiEB2828.
EcoGeneiEG13006. hybO.

Phylogenomic databases

eggNOGiENOG4105E3S. Bacteria.
COG1740. LUCA.
HOGENOMiHOG000278822.
InParanoidiP69741.
KOiK06282.
OMAiRSFNAHN.
PhylomeDBiP69741.

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-145.
ECOL316407:JW2965-MONOMER.
MetaCyc:MONOMER0-145.

Miscellaneous databases

PROiP69741.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
4.10.480.10. 1 hit.
InterProiIPR027394. Cytochrome-c3_hydrogenase_C.
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR001821. NiFe_hydrogenase_ssu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PANTHERiPTHR30013. PTHR30013. 1 hit.
PfamiPF14720. NiFe_hyd_SSU_C. 1 hit.
PF01058. Oxidored_q6. 1 hit.
[Graphical view]
PIRSFiPIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTSiPR00614. NIHGNASESMLL.
TIGRFAMsiTIGR00391. hydA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMBHT_ECOLI
AccessioniPrimary (citable) accession number: P69741
Secondary accession number(s): Q2M9J8, Q46847
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.