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Protein

Hydrogenase-2 small chain

Gene

hybO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake.

Catalytic activityi

H2 + A = AH2.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi59Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi62Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi157Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi191Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi229Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity1
Metal bindingi232Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi257Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi263Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi272Iron-sulfur 3 (3Fe-4S)By similarity1
Metal bindingi292Iron-sulfur 3 (3Fe-4S)By similarity1
Metal bindingi295Iron-sulfur 3 (3Fe-4S)By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
Ligand3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-145
MetaCyc:MONOMER0-145

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase-2 small chain (EC:1.12.99.6)
Short name:
HYD2
Alternative name(s):
Membrane-bound hydrogenase 2 small subunit
NiFe hydrogenase
Gene namesi
Name:hybO
Synonyms:yghV
Ordered Locus Names:b2997, JW2965
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13006 hybO

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane, Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 37Tat-type signalPROSITE-ProRule annotation1 PublicationAdd BLAST37
ChainiPRO_000001343038 – 372Hydrogenase-2 small chainAdd BLAST335

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven. Can also be exported by the Sec system.

Proteomic databases

PaxDbiP69741
PRIDEiP69741

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Binary interactionsi

WithEntry#Exp.IntActNotes
hybEP0AAN15EBI-552619,EBI-552588

Protein-protein interaction databases

BioGridi4261299, 260 interactors
DIPiDIP-36024N
IntActiP69741, 17 interactors
MINTiP69741
STRINGi316385.ECDH10B_3174

Structurei

3D structure databases

ProteinModelPortaliP69741
SMRiP69741
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105E3S Bacteria
COG1740 LUCA
HOGENOMiHOG000278822
InParanoidiP69741
KOiK06282
OMAiVPKRGWM
PhylomeDBiP69741

Family and domain databases

Gene3Di3.40.50.700, 1 hit
4.10.480.10, 1 hit
InterProiView protein in InterPro
IPR027394 Cytochrome-c3_hydrogenase_C
IPR006137 NADH_UbQ_OxRdtase-like_20kDa
IPR037148 NiFe-Hase_small_C_sf
IPR037024 NiFe_Hase_small_N_sf
IPR001821 NiFe_hydrogenase_ssu
IPR006311 TAT_signal
IPR019546 TAT_signal_bac_arc
PANTHERiPTHR30013 PTHR30013, 1 hit
PfamiView protein in Pfam
PF14720 NiFe_hyd_SSU_C, 1 hit
PF01058 Oxidored_q6, 1 hit
PIRSFiPIRSF000310 NiFe_hyd_ssu, 1 hit
TIGRFAMsiTIGR00391 hydA, 1 hit
TIGR01409 TAT_signal_seq, 1 hit
PROSITEiView protein in PROSITE
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69741-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGDNTLIHS HGINRRDFMK LCAALAATMG LSSKAAAEMA ESVTNPQRPP
60 70 80 90 100
VIWIGAQECT GCTESLLRAT HPTVENLVLE TISLEYHEVL SAAFGHQVEE
110 120 130 140 150
NKHNALEKYK GQYVLVVDGS IPLKDNGIYC MVAGEPIVDH IRKAAEGAAA
160 170 180 190 200
IIAIGSCSAW GGVAAAGVNP TGAVSLQEVL PGKTVINIPG CPPNPHNFLA
210 220 230 240 250
TVAHIITYGK PPKLDDKNRP TFAYGRLIHE HCERRPHFDA GRFAKEFGDE
260 270 280 290 300
GHREGWCLYH LGCKGPETYG NCSTLQFCDV GGVWPVAIGH PCYGCNEEGI
310 320 330 340 350
GFHKGIHQLA NVENQTPRSQ KPDVNAKEGG NVSAGAIGLL GGVVGLVAGV
360 370
SVMAVRELGR QQKKDNADSR GE
Length:372
Mass (Da):39,652
Last modified:April 26, 2005 - v1
Checksum:iC5821096BBAF9C66
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28377 Genomic DNA Translation: AAA69164.1
U00096 Genomic DNA Translation: AAC76033.1
AP009048 Genomic DNA Translation: BAE77058.1
PIRiC65086
RefSeqiNP_417471.1, NC_000913.3
WP_000145410.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76033; AAC76033; b2997
BAE77058; BAE77058; BAE77058
GeneIDi945902
KEGGiecj:JW2965
eco:b2997
PATRICifig|1411691.4.peg.3732

Similar proteinsi

Entry informationi

Entry nameiMBHT_ECOLI
AccessioniPrimary (citable) accession number: P69741
Secondary accession number(s): Q2M9J8, Q46847
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: March 28, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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