Hydrogenase-2 small chain precursor - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P69741 (MBHT_ECOLI)

Last modified June 16, 2009. Version 40. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Hydrogenase-2 small chain
      Short name=HYD2
    EC=1.12.99.6
Alternative name(s):
    Membrane-bound hydrogenase 2 small subunit
    NiFe hydrogenase

Gene names

Name:	hybO
Synonyms:	yghV
Ordered Locus Names:	b2997, JW2965

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake.
Catalytic activity	H₂ + A = AH₂.
Cofactor	Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein; Periplasmic side.
Post-translational modification	Exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

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Ontologies

Keywords
Cellular component	Cell membrane Membrane Periplasm
Domain	Signal
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ferredoxin hydrogenase complex Inferred from electronic annotation. Source: InterPro periplasmic space Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
hybE	P0AAN1	2	EBI-552619,EBI-552588

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 37

Tat-type signal Ref.3

Chain

38 – 372

335

Hydrogenase-2 small chain

PRO_0000013430

Sites

Metal binding

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

157

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

191

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

229

Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity

Metal binding

232

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

257

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

263

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

272

Iron-sulfur 3 (3Fe-4S) By similarity

Metal binding

292

Iron-sulfur 3 (3Fe-4S) By similarity

Metal binding

295

Iron-sulfur 3 (3Fe-4S) By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

P69741-1 [UniParc].

Last modified April 26, 2005. Version 1.
Checksum: C5821096BBAF9C66
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FASTA

372

39,652

        10         20         30         40         50         60 
MTGDNTLIHS HGINRRDFMK LCAALAATMG LSSKAAAEMA ESVTNPQRPP VIWIGAQECT 

        70         80         90        100        110        120 
GCTESLLRAT HPTVENLVLE TISLEYHEVL SAAFGHQVEE NKHNALEKYK GQYVLVVDGS 

       130        140        150        160        170        180 
IPLKDNGIYC MVAGEPIVDH IRKAAEGAAA IIAIGSCSAW GGVAAAGVNP TGAVSLQEVL 

       190        200        210        220        230        240 
PGKTVINIPG CPPNPHNFLA TVAHIITYGK PPKLDDKNRP TFAYGRLIHE HCERRPHFDA 

       250        260        270        280        290        300 
GRFAKEFGDE GHREGWCLYH LGCKGPETYG NCSTLQFCDV GGVWPVAIGH PCYGCNEEGI 

       310        320        330        340        350        360 
GFHKGIHQLA NVENQTPRSQ KPDVNAKEGG NVSAGAIGLL GGVVGLVAGV SVMAVRELGR 

       370 
QQKKDNADSR GE

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Reassignment of the gene encoding the Escherichia coli hydrogenase 2 small subunit -- identification of a soluble precursor of the small subunit in a hypB mutant." Sargent F., Ballantine S.P., Rugman P.A., Palmer T., Boxer D.H. Eur. J. Biochem. 255:746-754(1998) [PubMed: 9738917] [Abstract] Cited for: PROTEIN SEQUENCE OF 38-47 AND 218-234, CHARACTERIZATION, EXPORT VIA THE TAT-SYSTEM. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

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Cross-references

Sequence databases
	U28377 Genomic DNA. Translation: AAA69164.1. U00096 Genomic DNA. Translation: AAC76033.1. AP009048 Genomic DNA. Translation: BAE77058.1.
PIR	C65086.
RefSeq	AP_003552.1. NP_417471.1.
3D structure databases
HSSP	HSSP built from PDB template 1FRF based on UniProtKB P18187.
ModBase	Search...
Protein-protein interaction databases
IntAct	P69741. 6 interactions.
Genome annotation databases
GeneID	945902.
GenomeReviews	Gene locus JW2965 in contig AP009048_GR. Gene locus b2997 in contig U00096_GR.
KEGG	ecj:JW2965. eco:b2997.
Organism-specific databases
EchoBASE	EB2828.
EcoGene	EG13006. hybO.
CMR	Search...
Phylogenomic databases
HOGENOM	P69741.
OMA	P69741. RLIHENC.
Enzyme and pathway databases
BioCyc	EcoCyc:MON0-145. MetaCyc:MON0-145.
Family and domain databases
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001821. NiFe_hydrogenase_ssu. IPR006311. Tat. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
PIRSF	PIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTS	PR00614. NIHGNASESMLL.
TIGRFAMs	TIGR00391. hydA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

MBHT_ECOLI

Accession

Primary (citable) accession number: P69741
Secondary accession number(s): Q2M9J8, Q46847

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	April 26, 2005
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents