Reviewed, UniProtKB/Swiss-Prot P69741 (MBHT_ECOLI)

Names and origin

Protein names

Recommended name:
    Hydrogenase-2 small chain
      Short name=HYD2
    EC=1.12.99.6
Alternative name(s):
    Membrane-bound hydrogenase 2 small subunit
    NiFe hydrogenase

Gene names

Name:	hybO
Synonyms:	yghV
Ordered Locus Names:	b2997, JW2965

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake.
Catalytic activity	H(2) + A = AH(2).
Cofactor	Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein; Periplasmic side.
Post-translational modification	Exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Periplasm
Domain	Signal
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	mitochondrial electron transport, NADH to ubiquinone Inferred from electronic annotation. Source: InterPro
Cellular component	ferredoxin hydrogenase complex Inferred from electronic annotation. Source: InterPro periplasmic space Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
hybE	P0AAN1	2	EBI-552619,EBI-552588

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 37	37	Tat-type signal
	Chain	38 – 372	335	Hydrogenase-2 small chain		PRO_0000013430
Sites
	Metal binding	59	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	62	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	157	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	191	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	229	1	Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
	Metal binding	232	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	257	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	263	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	272	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	292	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	295	1	Iron-sulfur 3 (3Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P69741-1 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: C5821096BBAF9C66	FASTA	372	39,652
	10 20 30 40 50 60 MTGDNTLIHS HGINRRDFMK LCAALAATMG LSSKAAAEMA ESVTNPQRPP VIWIGAQECT 70 80 90 100 110 120 GCTESLLRAT HPTVENLVLE TISLEYHEVL SAAFGHQVEE NKHNALEKYK GQYVLVVDGS 130 140 150 160 170 180 IPLKDNGIYC MVAGEPIVDH IRKAAEGAAA IIAIGSCSAW GGVAAAGVNP TGAVSLQEVL 190 200 210 220 230 240 PGKTVINIPG CPPNPHNFLA TVAHIITYGK PPKLDDKNRP TFAYGRLIHE HCERRPHFDA 250 260 270 280 290 300 GRFAKEFGDE GHREGWCLYH LGCKGPETYG NCSTLQFCDV GGVWPVAIGH PCYGCNEEGI 310 320 330 340 350 360 GFHKGIHQLA NVENQTPRSQ KPDVNAKEGG NVSAGAIGLL GGVVGLVAGV SVMAVRELGR 370 QQKKDNADSR GE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Reassignment of the gene encoding the Escherichia coli hydrogenase 2 small subunit -- identification of a soluble precursor of the small subunit in a hypB mutant." Sargent F., Ballantine S.P., Rugman P.A., Palmer T., Boxer D.H. Eur. J. Biochem. 255:746-754(1998) [PubMed: 9738917] [Abstract] Cited for: PROTEIN SEQUENCE OF 38-47 AND 218-234, CHARACTERIZATION, EXPORT VIA THE TAT-SYSTEM. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U28377 Genomic DNA. Translation: AAA69164.1. U00096 Genomic DNA. Translation: AAC76033.1. AP009048 Genomic DNA. Translation: BAE77058.1.
PIR	C65086.
RefSeq	AP_003552.1. NP_417471.1.
3D structure databases
HSSP	HSSP built from PDB template 1FRF based on UniProtKB P18187.
ModBase	Search...
Protein-protein interaction databases
IntAct	P69741.
Genome annotation databases
GeneID	945902.
GenomeReviews	Gene locus b2997 in contig U00096_GR. Gene locus JW2965 in contig AP009048_GR.
KEGG	ecj:JW2965. eco:b2997.
Organism-specific databases
EchoBASE	EB2828.
EcoGene	EG13006. hybO.
CMR	Search...
Phylogenomic databases
HOGENOM	P69741.
Enzyme and pathway databases
BioCyc	EcoCyc:MON0-145. MetaCyc:MON0-145.
Family and domain databases
InterPro	IPR001821. NiFe_hyd_ssu. IPR013634. NiFe_hyd_ssu_N. IPR006137. OxRdtase_q6. IPR006311. Tat. [Graphical view]
Pfam	PF08425. NiFe_dehyd_N. 1 hit. PF01058. Oxidored_q6. 1 hit. [Graphical view]
PIRSF	PIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTS	PR00614. NIHGNASESMLL.
TIGRFAMs	TIGR00391. hydA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MBHT_ECOLI

Accession

Primary (citable) accession number: P69741
Secondary accession number(s): Q2M9J8, Q46847

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	April 26, 2005
Last modified:	November 25, 2008
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)