Hydrogenase-1 small chain precursor

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P69740 (MBHS_ECOL6) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 54. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Hydrogenase-1 small chain
Short name=HYD1
EC=1.12.99.6

Alternative name(s):
Membrane-bound hydrogenase 1 small subunit
NiFe hydrogenase

Gene names

Name:	hyaA
Ordered Locus Names:	c1113

Organism

Escherichia coli O6 [Complete proteome] [HAMAP]

Taxonomic identifier

217992 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD1 is believed to have a role in hydrogen cycling during fermentative growth.
Catalytic activity	H₂ + A = AH₂.
Cofactor	Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell inner membrane; Single-pass type I membrane protein; Periplasmic side By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.
Sequence caution	The sequence AAN79581.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	ferredoxin hydrogenase complex Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 45

Tat-type signal Potential

Chain

46 – 372

327

Hydrogenase-1 small chain

PRO_0000013428

Regions

Topological domain

46 – 326

281

Periplasmic Potential

Transmembrane

327 – 347

Helical; Potential

Topological domain

348 – 372

Cytoplasmic Potential

Sites

Metal binding

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

160

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

194

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

232

Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity

Metal binding

235

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

260

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

266

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

275

Iron-sulfur 3 (3Fe-4S) By similarity

Metal binding

294

Iron-sulfur 3 (3Fe-4S) By similarity

Metal binding

297

Iron-sulfur 3 (3Fe-4S) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P69740 [UniParc].

Last modified April 26, 2005. Version 1.
Checksum: 4AD3ECB4220D2826
Show »

FASTA

372

40,681

        10         20         30         40         50         60 
MNNEETFYQA MRRQGVTRRS FLKYCSLAAT SLGLGAGMAP KIAWALENKP RIPVVWIHGL 

        70         80         90        100        110        120 
ECTCCTESFI RSAHPLAKDV ILSLISLDYD DTLMAAAGTQ AEEVFEDIIT QYNGKYILAV 

       130        140        150        160        170        180 
EGNPPLGEQG MFCISSGRPF IEKLKRAAAG ASAIIAWGTC ASWGCVQAAR PNPTQATPID 

       190        200        210        220        230        240 
KVITDKPIIK VPGCPPIPDV MSAIITYMVT FDRLPDVDRM GRPLMFYGQR IHDKCYRRAH 

       250        260        270        280        290        300 
FDAGEFVQSW DDDAARKGYC LYKMGCKGPT TYNACSSTRW NDGVSFPIQS GHGCLGCAEN 

       310        320        330        340        350        360 
GFWDRGSFYS RVVDIPQMGT HSTADTVGLT ALGVVAAAVG VHAVASAVDQ RRRHNQQPTE 

       370 
TEHQPGNEDK QA

« Hide

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli."
Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R.
Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed: 12471157] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O6:H1 / CFT073 / ATCC 700928 / UPEC.

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014075 Genomic DNA. Translation: AAN79581.1. Different initiation.
RefSeq	NP_753038.1. NC_004431.1.
3D structure databases
ProteinModelPortal	P69740.
SMR	P69740. Positions 49-316.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000040478; EBESCP00000038827; EBESCG00000039528.
GeneID	1040006.
GenomeReviews	Gene locus c1113 in contig AE014075_GR.
KEGG	ecc:c1113.
PATRIC	18280254. VBIEscCol75197_1054.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000010145.
HOGENOM	HBG575615.
OMA	LEYHETV.
ProtClustDB	CLSK879888.
Family and domain databases
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001821. NiFe_hydrogenase_ssu. IPR006311. TAT_signal. IPR019546. TAT_signal_bac_arc. [Graphical view]
Gene3D	G3DSA:3.40.50.700. G3DSA:3.40.50.700. 1 hit.
KO	K06282.
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
PIRSF	PIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTS	PR00614. NIHGNASESMLL.
TIGRFAMs	TIGR00391. HydA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MBHS_ECOL6

Accession

Primary (citable) accession number: P69740
Secondary accession number(s): P19928

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	April 26, 2005
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents