Reviewed,
UniProtKB/Swiss-Prot P69739 (MBHS_ECOLI)
Last modified
November 3, 2009.
Version 43.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Hydrogenase-1 small chain Short name=HYD1 EC=1.12.99.6 Alternative name(s): Membrane-bound hydrogenase 1 small subunit NiFe hydrogenase | ||||
| Gene names |
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| Organism | Escherichia coli (strain K12) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 83333 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 372 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD1 is believed to have a role in hydrogen cycling during fermentative growth. |
| Catalytic activity | H2 + A = AH2. |
| Cofactor | Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity. |
| Subunit structure | Heterodimer of a large and a small subunit. |
| Subcellular location | Cell inner membrane; Single-pass type I membrane protein; Periplasmic side. |
| Post-translational modification | Exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven. |
| Sequence similarities | Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 45 | 45 | Tat-type signal Ref.1 | ||||||
| Chain | 46 – 372 | 327 | Hydrogenase-1 small chain | PRO_0000013427 | |||||
Regions | |||||||||
| Topological domain | 46 – 325 | 280 | Periplasmic Potential | ||||||
| Transmembrane | 326 – 348 | 23 | Potential | ||||||
| Topological domain | 349 – 372 | 24 | Cytoplasmic Potential | ||||||
Sites | |||||||||
| Metal binding | 62 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 65 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 160 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 194 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 232 | 1 | Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity | ||||||
| Metal binding | 235 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 260 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 266 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 275 | 1 | Iron-sulfur 3 (3Fe-4S) By similarity | ||||||
| Metal binding | 294 | 1 | Iron-sulfur 3 (3Fe-4S) By similarity | ||||||
| Metal binding | 297 | 1 | Iron-sulfur 3 (3Fe-4S) By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames." Menon N.K., Robbins J., Peck H.D. Jr., Chatelus C.Y., Choi E.-S., Przybyla A.E. J. Bacteriol. 172:1969-1977(1990) [PubMed: 2180913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 46-50. |
| [2] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [3] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [4] | "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T.DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-57. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [5] | "Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076. |
| [6] | "Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides." Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B., Palmer T., Georgiou G. J. Biol. Chem. 282:8309-8316(2007) [PubMed: 17218314] [Abstract] Cited for: EXPORT VIA THE TAT-SYSTEM. |
Cross-references
Sequence databases | |
|---|---|
| M34825 Genomic DNA. Translation: AAA23997.1. U00096 Genomic DNA. Translation: AAC74057.1. AP009048 Genomic DNA. Translation: BAA35737.2. | |
| PIR | HQECSN. JV0072. |
| RefSeq | AP_001601.1. NP_415491.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1FRF based on UniProtKB P18187. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P69739. |
Genome annotation databases | |
| GeneID | 945579. |
| GenomeReviews | Gene locus JW0954 in contig AP009048_GR. Gene locus b0972 in contig U00096_GR. |
| KEGG | ecj:JW0954. eco:b0972. |
Organism-specific databases | |
| EchoBASE | EB0463. |
| EcoGene | EG10468. hyaA. |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P69739. |
| OMA | LEYHETV. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:HYAA-MON. MetaCyc:HYAA-MON. |
Gene expression databases | |
| Genevestigator | P69739. |
Family and domain databases | |
| InterPro | IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001821. NiFe_hydrogenase_ssu. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view] |
| Pfam | PF01058. Oxidored_q6. 1 hit. [Graphical view] |
| PIRSF | PIRSF000310. NiFe_hyd_ssu. 1 hit. |
| PRINTS | PR00614. NIHGNASESMLL. |
| TIGRFAMs | TIGR00391. hydA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit. |
| PROSITE | PS51318. TAT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MBHS_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P69739 Secondary accession number(s): P19928 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| SIMILARITY comments Index of protein domains and families |

Clusters with


