Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P69739

- MBHS_ECOLI

UniProt

P69739 - MBHS_ECOLI

Protein

Hydrogenase-1 small chain

Gene

hyaA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (26 Apr 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD1 is believed to have a role in hydrogen cycling during fermentative growth.

    Catalytic activityi

    H2 + A = AH2.

    Cofactori

    Binds 1 3Fe-4S cluster.By similarity
    Binds 2 4Fe-4S clusters.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi62 – 621Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi160 – 1601Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi194 – 1941Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi232 – 2321Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity
    Metal bindingi235 – 2351Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi260 – 2601Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi266 – 2661Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi275 – 2751Iron-sulfur 3 (3Fe-4S)By similarity
    Metal bindingi294 – 2941Iron-sulfur 3 (3Fe-4S)By similarity
    Metal bindingi297 – 2971Iron-sulfur 3 (3Fe-4S)By similarity

    GO - Molecular functioni

    1. 3 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. ferredoxin hydrogenase activity Source: InterPro
    4. hydrogenase (acceptor) activity Source: UniProtKB-EC
    5. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:HYAA-MONOMER.
    ECOL316407:JW0954-MONOMER.
    MetaCyc:HYAA-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydrogenase-1 small chain (EC:1.12.99.6)
    Short name:
    HYD1
    Alternative name(s):
    Membrane-bound hydrogenase 1 small subunit
    NiFe hydrogenase
    Gene namesi
    Name:hyaA
    Ordered Locus Names:b0972, JW0954
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10468. hyaA.

    Subcellular locationi

    GO - Cellular componenti

    1. ferredoxin hydrogenase complex Source: InterPro
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 4545Tat-type signal1 PublicationPROSITE-ProRule annotationAdd
    BLAST
    Chaini46 – 372327Hydrogenase-1 small chainPRO_0000013427Add
    BLAST

    Post-translational modificationi

    Exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

    Expressioni

    Gene expression databases

    GenevestigatoriP69739.

    Interactioni

    Subunit structurei

    Heterodimer of a large and a small subunit.

    Protein-protein interaction databases

    DIPiDIP-47848N.
    IntActiP69739. 2 interactions.
    STRINGi511145.b0972.

    Structurei

    Secondary structure

    1
    372
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi52 – 587
    Helixi64 – 707
    Turni73 – 764
    Helixi77 – 837
    Beta strandi85 – 906
    Turni91 – 933
    Helixi98 – 11114
    Turni112 – 1154
    Beta strandi116 – 1238
    Helixi127 – 1304
    Beta strandi133 – 1353
    Helixi140 – 15011
    Beta strandi151 – 1577
    Helixi158 – 1625
    Helixi165 – 1684
    Helixi179 – 1813
    Beta strandi188 – 1914
    Beta strandi193 – 1953
    Helixi198 – 21114
    Helixi224 – 2274
    Beta strandi228 – 2303
    Helixi231 – 2333
    Helixi238 – 2436
    Helixi255 – 2573
    Helixi262 – 2643
    Helixi268 – 2703
    Beta strandi272 – 2743
    Turni275 – 2773
    Turni281 – 2844
    Turni287 – 2915
    Helixi302 – 3043
    Helixi320 – 33819
    Beta strandi346 – 3494

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3UQYX-ray1.47S/T46-372[»]
    3USCX-ray2.00S/T46-372[»]
    3USEX-ray1.67S/T46-372[»]
    4GD3X-ray3.30Q/R/S/T46-372[»]
    ProteinModelPortaliP69739.
    SMRiP69739. Positions 49-352.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini46 – 325280PeriplasmicSequence AnalysisAdd
    BLAST
    Topological domaini349 – 37224CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei326 – 34823HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1740.
    HOGENOMiHOG000278822.
    KOiK06282.
    OMAiMAEDAMA.
    OrthoDBiEOG6J74S9.
    PhylomeDBiP69739.

    Family and domain databases

    Gene3Di3.40.50.700. 1 hit.
    4.10.480.10. 1 hit.
    InterProiIPR027394. Cytochrome-c3_hydrogenase_C.
    IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
    IPR001821. NiFe_hydrogenase_ssu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF14720. NiFe_hyd_SSU_C. 1 hit.
    PF01058. Oxidored_q6. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000310. NiFe_hyd_ssu. 1 hit.
    PRINTSiPR00614. NIHGNASESMLL.
    TIGRFAMsiTIGR00391. hydA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P69739-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNEETFYQA MRRQGVTRRS FLKYCSLAAT SLGLGAGMAP KIAWALENKP    50
    RIPVVWIHGL ECTCCTESFI RSAHPLAKDV ILSLISLDYD DTLMAAAGTQ 100
    AEEVFEDIIT QYNGKYILAV EGNPPLGEQG MFCISSGRPF IEKLKRAAAG 150
    ASAIIAWGTC ASWGCVQAAR PNPTQATPID KVITDKPIIK VPGCPPIPDV 200
    MSAIITYMVT FDRLPDVDRM GRPLMFYGQR IHDKCYRRAH FDAGEFVQSW 250
    DDDAARKGYC LYKMGCKGPT TYNACSSTRW NDGVSFPIQS GHGCLGCAEN 300
    GFWDRGSFYS RVVDIPQMGT HSTADTVGLT ALGVVAAAVG VHAVASAVDQ 350
    RRRHNQQPTE TEHQPGNEDK QA 372
    Length:372
    Mass (Da):40,681
    Last modified:April 26, 2005 - v1
    Checksum:i4AD3ECB4220D2826
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34825 Genomic DNA. Translation: AAA23997.1.
    U00096 Genomic DNA. Translation: AAC74057.1.
    AP009048 Genomic DNA. Translation: BAA35737.2.
    PIRiJV0072. HQECSN.
    RefSeqiNP_415491.1. NC_000913.3.
    YP_489243.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74057; AAC74057; b0972.
    BAA35737; BAA35737; BAA35737.
    GeneIDi12932807.
    945579.
    KEGGiecj:Y75_p0943.
    eco:b0972.
    PATRICi32117165. VBIEscCol129921_1006.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34825 Genomic DNA. Translation: AAA23997.1 .
    U00096 Genomic DNA. Translation: AAC74057.1 .
    AP009048 Genomic DNA. Translation: BAA35737.2 .
    PIRi JV0072. HQECSN.
    RefSeqi NP_415491.1. NC_000913.3.
    YP_489243.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3UQY X-ray 1.47 S/T 46-372 [» ]
    3USC X-ray 2.00 S/T 46-372 [» ]
    3USE X-ray 1.67 S/T 46-372 [» ]
    4GD3 X-ray 3.30 Q/R/S/T 46-372 [» ]
    ProteinModelPortali P69739.
    SMRi P69739. Positions 49-352.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-47848N.
    IntActi P69739. 2 interactions.
    STRINGi 511145.b0972.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74057 ; AAC74057 ; b0972 .
    BAA35737 ; BAA35737 ; BAA35737 .
    GeneIDi 12932807.
    945579.
    KEGGi ecj:Y75_p0943.
    eco:b0972.
    PATRICi 32117165. VBIEscCol129921_1006.

    Organism-specific databases

    EchoBASEi EB0463.
    EcoGenei EG10468. hyaA.

    Phylogenomic databases

    eggNOGi COG1740.
    HOGENOMi HOG000278822.
    KOi K06282.
    OMAi MAEDAMA.
    OrthoDBi EOG6J74S9.
    PhylomeDBi P69739.

    Enzyme and pathway databases

    BioCyci EcoCyc:HYAA-MONOMER.
    ECOL316407:JW0954-MONOMER.
    MetaCyc:HYAA-MONOMER.

    Miscellaneous databases

    PROi P69739.

    Gene expression databases

    Genevestigatori P69739.

    Family and domain databases

    Gene3Di 3.40.50.700. 1 hit.
    4.10.480.10. 1 hit.
    InterProi IPR027394. Cytochrome-c3_hydrogenase_C.
    IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
    IPR001821. NiFe_hydrogenase_ssu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF14720. NiFe_hyd_SSU_C. 1 hit.
    PF01058. Oxidored_q6. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000310. NiFe_hyd_ssu. 1 hit.
    PRINTSi PR00614. NIHGNASESMLL.
    TIGRFAMsi TIGR00391. hydA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames."
      Menon N.K., Robbins J., Peck H.D. Jr., Chatelus C.Y., Choi E.-S., Przybyla A.E.
      J. Bacteriol. 172:1969-1977(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 46-50.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-57.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Global topology analysis of the Escherichia coli inner membrane proteome."
      Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
      Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: K12 / MG1655 / ATCC 47076.
    6. "Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides."
      Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B., Palmer T., Georgiou G.
      J. Biol. Chem. 282:8309-8316(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: EXPORT VIA THE TAT-SYSTEM.

    Entry informationi

    Entry nameiMBHS_ECOLI
    AccessioniPrimary (citable) accession number: P69739
    Secondary accession number(s): P19928
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3