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Protein

Hydrogenase-1 small chain

Gene

hyaA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD1 is believed to have a role in hydrogen cycling during fermentative growth.

Catalytic activityi

H2 + A = AH2.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi62Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi65Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi160Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi194Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi232Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity1
Metal bindingi235Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi260Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi266Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi275Iron-sulfur 3 (3Fe-4S)By similarity1
Metal bindingi294Iron-sulfur 3 (3Fe-4S)By similarity1
Metal bindingi297Iron-sulfur 3 (3Fe-4S)By similarity1

GO - Molecular functioni

  • 3 iron, 4 sulfur cluster binding Source: EcoCyc
  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • electron carrier activity Source: EcoCyc
  • ferredoxin hydrogenase activity Source: InterPro
  • hydrogenase (acceptor) activity Source: UniProtKB-EC
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • anaerobic respiration Source: EcoCyc
  • fermentation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:HYAA-MONOMER.
ECOL316407:JW0954-MONOMER.
MetaCyc:HYAA-MONOMER.
BRENDAi1.12.99.6. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase-1 small chain (EC:1.12.99.6)
Short name:
HYD1
Alternative name(s):
Membrane-bound hydrogenase 1 small subunit
NiFe hydrogenase
Gene namesi
Name:hyaA
Ordered Locus Names:b0972, JW0954
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10468. hyaA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini46 – 325PeriplasmicSequence analysisAdd BLAST280
Transmembranei326 – 348HelicalSequence analysisAdd BLAST23
Topological domaini349 – 372CytoplasmicSequence analysisAdd BLAST24

GO - Cellular componenti

  • [Ni-Fe] hydrogenase complex Source: EcoCyc
  • ferredoxin hydrogenase complex Source: InterPro
  • integral component of membrane Source: EcoCyc
  • intrinsic component of periplasmic side of plasma membrane Source: EcoCyc
  • membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 45Tat-type signalPROSITE-ProRule annotation1 PublicationAdd BLAST45
ChainiPRO_000001342746 – 372Hydrogenase-1 small chainAdd BLAST327

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Proteomic databases

PaxDbiP69739.
PRIDEiP69739.

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Protein-protein interaction databases

BioGridi4261801. 9 interactors.
DIPiDIP-47848N.
IntActiP69739. 2 interactors.
STRINGi511145.b0972.

Structurei

Secondary structure

1372
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi52 – 58Combined sources7
Helixi64 – 70Combined sources7
Turni73 – 76Combined sources4
Helixi77 – 83Combined sources7
Beta strandi85 – 90Combined sources6
Turni91 – 93Combined sources3
Helixi98 – 111Combined sources14
Turni112 – 115Combined sources4
Beta strandi116 – 123Combined sources8
Helixi127 – 130Combined sources4
Beta strandi133 – 135Combined sources3
Helixi140 – 150Combined sources11
Beta strandi151 – 157Combined sources7
Helixi158 – 162Combined sources5
Helixi166 – 168Combined sources3
Helixi179 – 181Combined sources3
Beta strandi188 – 191Combined sources4
Beta strandi193 – 195Combined sources3
Helixi198 – 211Combined sources14
Helixi224 – 227Combined sources4
Beta strandi228 – 230Combined sources3
Helixi231 – 233Combined sources3
Helixi238 – 243Combined sources6
Helixi255 – 257Combined sources3
Helixi262 – 264Combined sources3
Helixi268 – 270Combined sources3
Beta strandi272 – 274Combined sources3
Turni275 – 277Combined sources3
Turni281 – 284Combined sources4
Turni287 – 291Combined sources5
Helixi302 – 304Combined sources3
Helixi320 – 338Combined sources19
Beta strandi346 – 349Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UQYX-ray1.47S/T46-372[»]
3USCX-ray2.00S/T46-372[»]
3USEX-ray1.67S/T46-372[»]
4GD3X-ray3.30Q/R/S/T46-372[»]
4UE3X-ray1.40S/T46-372[»]
5A4FX-ray1.25S/T46-372[»]
5A4IX-ray1.23S/T46-372[»]
5A4MX-ray1.70S/T46-311[»]
5ADUX-ray1.10S/T46-372[»]
ProteinModelPortaliP69739.
SMRiP69739.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105E3S. Bacteria.
COG1740. LUCA.
HOGENOMiHOG000278822.
InParanoidiP69739.
KOiK06282.
OMAiMFVESFD.
PhylomeDBiP69739.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
4.10.480.10. 1 hit.
InterProiIPR027394. Cytochrome-c3_hydrogenase_C.
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR001821. NiFe_hydrogenase_ssu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PANTHERiPTHR30013. PTHR30013. 1 hit.
PfamiPF14720. NiFe_hyd_SSU_C. 1 hit.
PF01058. Oxidored_q6. 1 hit.
[Graphical view]
PIRSFiPIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTSiPR00614. NIHGNASESMLL.
TIGRFAMsiTIGR00391. hydA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69739-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNEETFYQA MRRQGVTRRS FLKYCSLAAT SLGLGAGMAP KIAWALENKP
60 70 80 90 100
RIPVVWIHGL ECTCCTESFI RSAHPLAKDV ILSLISLDYD DTLMAAAGTQ
110 120 130 140 150
AEEVFEDIIT QYNGKYILAV EGNPPLGEQG MFCISSGRPF IEKLKRAAAG
160 170 180 190 200
ASAIIAWGTC ASWGCVQAAR PNPTQATPID KVITDKPIIK VPGCPPIPDV
210 220 230 240 250
MSAIITYMVT FDRLPDVDRM GRPLMFYGQR IHDKCYRRAH FDAGEFVQSW
260 270 280 290 300
DDDAARKGYC LYKMGCKGPT TYNACSSTRW NDGVSFPIQS GHGCLGCAEN
310 320 330 340 350
GFWDRGSFYS RVVDIPQMGT HSTADTVGLT ALGVVAAAVG VHAVASAVDQ
360 370
RRRHNQQPTE TEHQPGNEDK QA
Length:372
Mass (Da):40,681
Last modified:April 26, 2005 - v1
Checksum:i4AD3ECB4220D2826
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34825 Genomic DNA. Translation: AAA23997.1.
U00096 Genomic DNA. Translation: AAC74057.1.
AP009048 Genomic DNA. Translation: BAA35737.2.
PIRiJV0072. HQECSN.
RefSeqiNP_415491.1. NC_000913.3.
WP_001058323.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74057; AAC74057; b0972.
BAA35737; BAA35737; BAA35737.
GeneIDi945579.
KEGGiecj:JW0954.
eco:b0972.
PATRICi32117165. VBIEscCol129921_1006.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34825 Genomic DNA. Translation: AAA23997.1.
U00096 Genomic DNA. Translation: AAC74057.1.
AP009048 Genomic DNA. Translation: BAA35737.2.
PIRiJV0072. HQECSN.
RefSeqiNP_415491.1. NC_000913.3.
WP_001058323.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UQYX-ray1.47S/T46-372[»]
3USCX-ray2.00S/T46-372[»]
3USEX-ray1.67S/T46-372[»]
4GD3X-ray3.30Q/R/S/T46-372[»]
4UE3X-ray1.40S/T46-372[»]
5A4FX-ray1.25S/T46-372[»]
5A4IX-ray1.23S/T46-372[»]
5A4MX-ray1.70S/T46-311[»]
5ADUX-ray1.10S/T46-372[»]
ProteinModelPortaliP69739.
SMRiP69739.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261801. 9 interactors.
DIPiDIP-47848N.
IntActiP69739. 2 interactors.
STRINGi511145.b0972.

Proteomic databases

PaxDbiP69739.
PRIDEiP69739.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74057; AAC74057; b0972.
BAA35737; BAA35737; BAA35737.
GeneIDi945579.
KEGGiecj:JW0954.
eco:b0972.
PATRICi32117165. VBIEscCol129921_1006.

Organism-specific databases

EchoBASEiEB0463.
EcoGeneiEG10468. hyaA.

Phylogenomic databases

eggNOGiENOG4105E3S. Bacteria.
COG1740. LUCA.
HOGENOMiHOG000278822.
InParanoidiP69739.
KOiK06282.
OMAiMFVESFD.
PhylomeDBiP69739.

Enzyme and pathway databases

BioCyciEcoCyc:HYAA-MONOMER.
ECOL316407:JW0954-MONOMER.
MetaCyc:HYAA-MONOMER.
BRENDAi1.12.99.6. 2026.

Miscellaneous databases

PROiP69739.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
4.10.480.10. 1 hit.
InterProiIPR027394. Cytochrome-c3_hydrogenase_C.
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR001821. NiFe_hydrogenase_ssu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PANTHERiPTHR30013. PTHR30013. 1 hit.
PfamiPF14720. NiFe_hyd_SSU_C. 1 hit.
PF01058. Oxidored_q6. 1 hit.
[Graphical view]
PIRSFiPIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTSiPR00614. NIHGNASESMLL.
TIGRFAMsiTIGR00391. hydA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMBHS_ECOLI
AccessioniPrimary (citable) accession number: P69739
Secondary accession number(s): P19928
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.