Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P69739 (MBHS_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydrogenase-1 small chain

Short name=HYD1
EC=1.12.99.6
Alternative name(s):
Membrane-bound hydrogenase 1 small subunit
NiFe hydrogenase
Gene names
Name:hyaA
Ordered Locus Names:b0972, JW0954
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD1 is believed to have a role in hydrogen cycling during fermentative growth.

Catalytic activity

H2 + A = AH2.

Cofactor

Binds 1 3Fe-4S cluster By similarity.

Binds 2 4Fe-4S clusters By similarity.

Subunit structure

Heterodimer of a large and a small subunit.

Subcellular location

Cell inner membrane; Single-pass type I membrane protein; Periplasmic side.

Post-translational modification

Exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Sequence similarities

Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4545Tat-type signal Ref.1
Chain46 – 372327Hydrogenase-1 small chain
PRO_0000013427

Regions

Topological domain46 – 325280Periplasmic Potential
Transmembrane326 – 34823Helical; Potential
Topological domain349 – 37224Cytoplasmic Potential

Sites

Metal binding621Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding651Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1601Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1941Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding2321Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
Metal binding2351Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2601Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2661Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2751Iron-sulfur 3 (3Fe-4S) By similarity
Metal binding2941Iron-sulfur 3 (3Fe-4S) By similarity
Metal binding2971Iron-sulfur 3 (3Fe-4S) By similarity

Secondary structure

.......................................................... 372
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P69739 [UniParc].

Last modified April 26, 2005. Version 1.
Checksum: 4AD3ECB4220D2826

FASTA37240,681
        10         20         30         40         50         60 
MNNEETFYQA MRRQGVTRRS FLKYCSLAAT SLGLGAGMAP KIAWALENKP RIPVVWIHGL 

        70         80         90        100        110        120 
ECTCCTESFI RSAHPLAKDV ILSLISLDYD DTLMAAAGTQ AEEVFEDIIT QYNGKYILAV 

       130        140        150        160        170        180 
EGNPPLGEQG MFCISSGRPF IEKLKRAAAG ASAIIAWGTC ASWGCVQAAR PNPTQATPID 

       190        200        210        220        230        240 
KVITDKPIIK VPGCPPIPDV MSAIITYMVT FDRLPDVDRM GRPLMFYGQR IHDKCYRRAH 

       250        260        270        280        290        300 
FDAGEFVQSW DDDAARKGYC LYKMGCKGPT TYNACSSTRW NDGVSFPIQS GHGCLGCAEN 

       310        320        330        340        350        360 
GFWDRGSFYS RVVDIPQMGT HSTADTVGLT ALGVVAAAVG VHAVASAVDQ RRRHNQQPTE 

       370 
TEHQPGNEDK QA 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames."
Menon N.K., Robbins J., Peck H.D. Jr., Chatelus C.Y., Choi E.-S., Przybyla A.E.
J. Bacteriol. 172:1969-1977(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 46-50.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-57.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.
[6]"Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides."
Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B., Palmer T., Georgiou G.
J. Biol. Chem. 282:8309-8316(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: EXPORT VIA THE TAT-SYSTEM.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M34825 Genomic DNA. Translation: AAA23997.1.
U00096 Genomic DNA. Translation: AAC74057.1.
AP009048 Genomic DNA. Translation: BAA35737.2.
PIRHQECSN. JV0072.
RefSeqNP_415491.1. NC_000913.3.
YP_489243.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3UQYX-ray1.47S/T46-372[»]
3USCX-ray2.00S/T46-372[»]
3USEX-ray1.67S/T46-372[»]
4GD3X-ray3.30Q/R/S/T46-372[»]
ProteinModelPortalP69739.
SMRP69739. Positions 49-352.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-47848N.
IntActP69739. 2 interactions.
STRING511145.b0972.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74057; AAC74057; b0972.
BAA35737; BAA35737; BAA35737.
GeneID12932807.
945579.
KEGGecj:Y75_p0943.
eco:b0972.
PATRIC32117165. VBIEscCol129921_1006.

Organism-specific databases

EchoBASEEB0463.
EcoGeneEG10468. hyaA.

Phylogenomic databases

eggNOGCOG1740.
HOGENOMHOG000278822.
KOK06282.
OMATRIHDKC.
OrthoDBEOG6J74S9.
PhylomeDBP69739.
ProtClustDBCLSK879888.

Enzyme and pathway databases

BioCycEcoCyc:HYAA-MONOMER.
ECOL316407:JW0954-MONOMER.
MetaCyc:HYAA-MONOMER.

Gene expression databases

GenevestigatorP69739.

Family and domain databases

Gene3D3.40.50.700. 1 hit.
4.10.480.10. 1 hit.
InterProIPR027394. Cytochrome-c3_hydrogenase_C.
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR001821. NiFe_hydrogenase_ssu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF14720. NiFe_hyd_SSU_C. 1 hit.
PF01058. Oxidored_q6. 1 hit.
[Graphical view]
PIRSFPIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTSPR00614. NIHGNASESMLL.
TIGRFAMsTIGR00391. hydA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP69739.

Entry information

Entry nameMBHS_ECOLI
AccessionPrimary (citable) accession number: P69739
Secondary accession number(s): P19928
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: April 16, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene