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P69739

- MBHS_ECOLI

UniProt

P69739 - MBHS_ECOLI

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Protein

Hydrogenase-1 small chain

Gene

hyaA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD1 is believed to have a role in hydrogen cycling during fermentative growth.

Catalytic activityi

H2 + A = AH2.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi160 – 1601Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi194 – 1941Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi232 – 2321Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity
Metal bindingi235 – 2351Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi260 – 2601Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi266 – 2661Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi275 – 2751Iron-sulfur 3 (3Fe-4S)By similarity
Metal bindingi294 – 2941Iron-sulfur 3 (3Fe-4S)By similarity
Metal bindingi297 – 2971Iron-sulfur 3 (3Fe-4S)By similarity

GO - Molecular functioni

  1. 3 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. ferredoxin hydrogenase activity Source: InterPro
  4. hydrogenase (acceptor) activity Source: UniProtKB-EC
  5. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:HYAA-MONOMER.
ECOL316407:JW0954-MONOMER.
MetaCyc:HYAA-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase-1 small chain (EC:1.12.99.6)
Short name:
HYD1
Alternative name(s):
Membrane-bound hydrogenase 1 small subunit
NiFe hydrogenase
Gene namesi
Name:hyaA
Ordered Locus Names:b0972, JW0954
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10468. hyaA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini46 – 325280PeriplasmicSequence AnalysisAdd
BLAST
Transmembranei326 – 34823HelicalSequence AnalysisAdd
BLAST
Topological domaini349 – 37224CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. ferredoxin hydrogenase complex Source: InterPro
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4545Tat-type signal1 PublicationPROSITE-ProRule annotationAdd
BLAST
Chaini46 – 372327Hydrogenase-1 small chainPRO_0000013427Add
BLAST

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Expressioni

Gene expression databases

GenevestigatoriP69739.

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Protein-protein interaction databases

DIPiDIP-47848N.
IntActiP69739. 2 interactions.
STRINGi511145.b0972.

Structurei

Secondary structure

1
372
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi52 – 587Combined sources
Helixi64 – 707Combined sources
Turni73 – 764Combined sources
Helixi77 – 837Combined sources
Beta strandi85 – 906Combined sources
Turni91 – 933Combined sources
Helixi98 – 11114Combined sources
Turni112 – 1154Combined sources
Beta strandi116 – 1238Combined sources
Helixi127 – 1304Combined sources
Beta strandi133 – 1353Combined sources
Helixi140 – 15011Combined sources
Beta strandi151 – 1577Combined sources
Helixi158 – 1625Combined sources
Helixi165 – 1684Combined sources
Helixi179 – 1813Combined sources
Beta strandi188 – 1914Combined sources
Beta strandi193 – 1953Combined sources
Helixi198 – 21114Combined sources
Helixi224 – 2274Combined sources
Beta strandi228 – 2303Combined sources
Helixi231 – 2333Combined sources
Helixi238 – 2436Combined sources
Helixi255 – 2573Combined sources
Helixi262 – 2643Combined sources
Helixi268 – 2703Combined sources
Beta strandi272 – 2743Combined sources
Turni275 – 2773Combined sources
Turni281 – 2844Combined sources
Turni287 – 2915Combined sources
Helixi302 – 3043Combined sources
Helixi320 – 33819Combined sources
Beta strandi346 – 3494Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3UQYX-ray1.47S/T46-372[»]
3USCX-ray2.00S/T46-372[»]
3USEX-ray1.67S/T46-372[»]
4GD3X-ray3.30Q/R/S/T46-372[»]
ProteinModelPortaliP69739.
SMRiP69739. Positions 49-352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1740.
HOGENOMiHOG000278822.
InParanoidiP69739.
KOiK06282.
OMAiMAEDAMA.
OrthoDBiEOG6J74S9.
PhylomeDBiP69739.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
4.10.480.10. 1 hit.
InterProiIPR027394. Cytochrome-c3_hydrogenase_C.
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR001821. NiFe_hydrogenase_ssu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF14720. NiFe_hyd_SSU_C. 1 hit.
PF01058. Oxidored_q6. 1 hit.
[Graphical view]
PIRSFiPIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTSiPR00614. NIHGNASESMLL.
TIGRFAMsiTIGR00391. hydA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69739-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNNEETFYQA MRRQGVTRRS FLKYCSLAAT SLGLGAGMAP KIAWALENKP
60 70 80 90 100
RIPVVWIHGL ECTCCTESFI RSAHPLAKDV ILSLISLDYD DTLMAAAGTQ
110 120 130 140 150
AEEVFEDIIT QYNGKYILAV EGNPPLGEQG MFCISSGRPF IEKLKRAAAG
160 170 180 190 200
ASAIIAWGTC ASWGCVQAAR PNPTQATPID KVITDKPIIK VPGCPPIPDV
210 220 230 240 250
MSAIITYMVT FDRLPDVDRM GRPLMFYGQR IHDKCYRRAH FDAGEFVQSW
260 270 280 290 300
DDDAARKGYC LYKMGCKGPT TYNACSSTRW NDGVSFPIQS GHGCLGCAEN
310 320 330 340 350
GFWDRGSFYS RVVDIPQMGT HSTADTVGLT ALGVVAAAVG VHAVASAVDQ
360 370
RRRHNQQPTE TEHQPGNEDK QA
Length:372
Mass (Da):40,681
Last modified:April 26, 2005 - v1
Checksum:i4AD3ECB4220D2826
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34825 Genomic DNA. Translation: AAA23997.1.
U00096 Genomic DNA. Translation: AAC74057.1.
AP009048 Genomic DNA. Translation: BAA35737.2.
PIRiJV0072. HQECSN.
RefSeqiNP_415491.1. NC_000913.3.
YP_489243.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74057; AAC74057; b0972.
BAA35737; BAA35737; BAA35737.
GeneIDi12932807.
945579.
KEGGiecj:Y75_p0943.
eco:b0972.
PATRICi32117165. VBIEscCol129921_1006.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34825 Genomic DNA. Translation: AAA23997.1 .
U00096 Genomic DNA. Translation: AAC74057.1 .
AP009048 Genomic DNA. Translation: BAA35737.2 .
PIRi JV0072. HQECSN.
RefSeqi NP_415491.1. NC_000913.3.
YP_489243.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3UQY X-ray 1.47 S/T 46-372 [» ]
3USC X-ray 2.00 S/T 46-372 [» ]
3USE X-ray 1.67 S/T 46-372 [» ]
4GD3 X-ray 3.30 Q/R/S/T 46-372 [» ]
ProteinModelPortali P69739.
SMRi P69739. Positions 49-352.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-47848N.
IntActi P69739. 2 interactions.
STRINGi 511145.b0972.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74057 ; AAC74057 ; b0972 .
BAA35737 ; BAA35737 ; BAA35737 .
GeneIDi 12932807.
945579.
KEGGi ecj:Y75_p0943.
eco:b0972.
PATRICi 32117165. VBIEscCol129921_1006.

Organism-specific databases

EchoBASEi EB0463.
EcoGenei EG10468. hyaA.

Phylogenomic databases

eggNOGi COG1740.
HOGENOMi HOG000278822.
InParanoidi P69739.
KOi K06282.
OMAi MAEDAMA.
OrthoDBi EOG6J74S9.
PhylomeDBi P69739.

Enzyme and pathway databases

BioCyci EcoCyc:HYAA-MONOMER.
ECOL316407:JW0954-MONOMER.
MetaCyc:HYAA-MONOMER.

Miscellaneous databases

PROi P69739.

Gene expression databases

Genevestigatori P69739.

Family and domain databases

Gene3Di 3.40.50.700. 1 hit.
4.10.480.10. 1 hit.
InterProi IPR027394. Cytochrome-c3_hydrogenase_C.
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR001821. NiFe_hydrogenase_ssu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF14720. NiFe_hyd_SSU_C. 1 hit.
PF01058. Oxidored_q6. 1 hit.
[Graphical view ]
PIRSFi PIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTSi PR00614. NIHGNASESMLL.
TIGRFAMsi TIGR00391. hydA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames."
    Menon N.K., Robbins J., Peck H.D. Jr., Chatelus C.Y., Choi E.-S., Przybyla A.E.
    J. Bacteriol. 172:1969-1977(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 46-50.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-57.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides."
    Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B., Palmer T., Georgiou G.
    J. Biol. Chem. 282:8309-8316(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: EXPORT VIA THE TAT-SYSTEM.

Entry informationi

Entry nameiMBHS_ECOLI
AccessioniPrimary (citable) accession number: P69739
Secondary accession number(s): P19928
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: November 26, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3