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Protein

Endothelial differentiation-related factor 1

Gene

Edf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. Might function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi92 – 11120H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

Calmodulin-binding, DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endothelial differentiation-related factor 1
Short name:
EDF-1
Alternative name(s):
Calmodulin-associated peptide 19
Short name:
CAP-19
Multiprotein-bridging factor 1
Short name:
MBF1
Gene namesi
Name:Edf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1308073. Edf1.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Also nuclear upon binding to NR5A1 and treatment of cells with TPA or forskolin (By similarity). Localized mainly in soma and dendritic processes of neurons.By similarity1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi87 – 871S → D: Loss of binding to CALM. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 148147Endothelial differentiation-related factor 1PRO_0000149797Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei4 – 41PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP69736.
PRIDEiP69736.

PTM databases

iPTMnetiP69736.

Expressioni

Tissue specificityi

Expressed in cardiomyocytes, cortical and hippocampal neurons.2 Publications

Developmental stagei

Expressed in newborn and adult heart and brain tissues. Expression is not evident in prenatal days.2 Publications

Inductioni

Up-regulated upon cardiomyocytes hypertrophy.

Gene expression databases

ExpressionAtlasiP69736. baseline.
GenevisibleiP69736. RN.

Interactioni

Subunit structurei

Interacts with TBP and the transcription factor IID (TFIID) complex, NR5A2, NR1H3 and PPARG. Interaction with TBP is regulated by phosphorylation. Binds NR5A1, ATF1 and FOS via their conserved basic region (By similarity). Interacts with JUN. Binding to calmodulin is regulated by calcium and phosphorylation of the IQ motif.By similarity2 Publications

Protein-protein interaction databases

BioGridi255402. 1 interaction.
IntActiP69736. 1 interaction.
MINTiMINT-4578369.
STRINGi10116.ENSRNOP00000022196.

Structurei

3D structure databases

ProteinModelPortaliP69736.
SMRiP69736. Positions 71-148.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 13555HTH cro/C1-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni37 – 11377Interaction with NR5A2, PPARG and NR1H3By similarityAdd
BLAST
Regioni69 – 10840Interaction with TBP and NR5A1By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi81 – 888IQ motif

Domaini

The IQ motif, which is involved in calmodulin binding, overlaps with the binding domain for nuclear receptors and transcription factors. Its phosphorylation probably allows a switch between the two activities of the protein.

Sequence similaritiesi

Contains 1 HTH cro/C1-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3398. Eukaryota.
COG1813. LUCA.
GeneTreeiENSGT00390000008519.
HOGENOMiHOG000195726.
HOVERGENiHBG051440.
InParanoidiP69736.
KOiK03627.
OMAiTKVNEKP.
OrthoDBiEOG7RFTJZ.
PhylomeDBiP69736.
TreeFamiTF300064.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
InterProiIPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
IPR013729. MBF1_N.
[Graphical view]
PfamiPF01381. HTH_3. 1 hit.
PF08523. MBF1. 1 hit.
[Graphical view]
SMARTiSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
PROSITEiPS50943. HTH_CROC1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69736-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAESDWDTVT VLRKKGPTAA QAKSKQAILA AQRRGEDVET SKKWAAGQNK
60 70 80 90 100
QHSITKNTAK LDRETEELHH DRVTLEVGKV IQRGRQSKGL TQKDLATKIN
110 120 130 140
EKPQVIADYE SGRAIPNNQV LGKIERAIGL KLRGKDIGKP IEKGPKAK
Length:148
Mass (Da):16,369
Last modified:April 12, 2005 - v1
Checksum:iADC000D8492A8C11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CO400850 mRNA. No translation available.
RefSeqiNP_001100027.1. NM_001106557.1.
UniGeneiRn.17092.

Genome annotation databases

EnsembliENSRNOT00000022196; ENSRNOP00000022196; ENSRNOG00000016272.
GeneIDi296570.
KEGGirno:296570.
UCSCiRGD:1308073. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CO400850 mRNA. No translation available.
RefSeqiNP_001100027.1. NM_001106557.1.
UniGeneiRn.17092.

3D structure databases

ProteinModelPortaliP69736.
SMRiP69736. Positions 71-148.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi255402. 1 interaction.
IntActiP69736. 1 interaction.
MINTiMINT-4578369.
STRINGi10116.ENSRNOP00000022196.

PTM databases

iPTMnetiP69736.

Proteomic databases

PaxDbiP69736.
PRIDEiP69736.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022196; ENSRNOP00000022196; ENSRNOG00000016272.
GeneIDi296570.
KEGGirno:296570.
UCSCiRGD:1308073. rat.

Organism-specific databases

CTDi8721.
RGDi1308073. Edf1.

Phylogenomic databases

eggNOGiKOG3398. Eukaryota.
COG1813. LUCA.
GeneTreeiENSGT00390000008519.
HOGENOMiHOG000195726.
HOVERGENiHBG051440.
InParanoidiP69736.
KOiK03627.
OMAiTKVNEKP.
OrthoDBiEOG7RFTJZ.
PhylomeDBiP69736.
TreeFamiTF300064.

Miscellaneous databases

PROiP69736.

Gene expression databases

ExpressionAtlasiP69736. baseline.
GenevisibleiP69736. RN.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
InterProiIPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
IPR013729. MBF1_N.
[Graphical view]
PfamiPF01381. HTH_3. 1 hit.
PF08523. MBF1. 1 hit.
[Graphical view]
SMARTiSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
PROSITEiPS50943. HTH_CROC1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a neuronal calmodulin-binding peptide, CAP-19, containing an IQ motif."
    Smith M.L., Johanson R.A., Rogers K.E., Coleman P.D., Slemmon J.R.
    Brain Res. Mol. Brain Res. 62:12-24(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH CALM.
    Tissue: Brain cortex.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Multiprotein bridging factor 1 cooperates with c-Jun and is necessary for cardiac hypertrophy in vitro."
    Busk P.K., Wulf-Andersen L., Stroem C.C., Enevoldsen M., Thirstrup K., Haunsoe S., Sheikh S.P.
    Exp. Cell Res. 286:102-114(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CALM AND JUN, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-87, FUNCTION.

Entry informationi

Entry nameiEDF1_RAT
AccessioniPrimary (citable) accession number: P69736
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: April 12, 2005
Last modified: July 6, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.