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P69732 (GAG_EIAVY) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gag polyprotein

Cleaved into the following 5 chains:

  1. Matrix protein p15
    Short name=MA
  2. Capsid protein p26
    Short name=CA
  3. p1
  4. Nucleocapsid protein p11
    Short name=NC
  5. p9
Gene names
Name:gag
OrganismEquine infectious anemia virus (strain Wyoming) (EIAV)
Taxonomic identifier11672 [NCBI]
Taxonomic lineageVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeLentivirusEquine lentivirus group
Virus hostEquus asinus (Donkey) [TaxID: 9793]
Equus caballus (Horse) [TaxID: 9796]

Protein attributes

Sequence length486 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Matrix protein p15 forms the outer shell of the core of the virus, lining the inner surface of the viral membrane By similarity.

Capsid protein p26 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex By similarity.

Nucleocapsid protein p11 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers By similarity.

p9 plays a role in budding of the assembled particle by interacting with PDCD6IP/AIP1 By similarity.

Subunit structure

p9 interacts with human PDCD6IP/AIP1 By similarity.

Subcellular location

Virion Potential.

Domain

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. p9 contains one L domain: a LYPX(n)L motif, which interacts with PDCD6IP/AIP1 By similarity.

Sequence similarities

Belongs to the equine lentivirus group gag polyprotein family.

Contains 2 CCHC-type zinc fingers.

Caution

The original EMBL accession numbers (M11337 and M14855) assigned to this isolate (isolate Wyoming) have been made secondary to M16575 which is from a different isolate (clone 1365).

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 124124Matrix protein p15
PRO_0000038780
Chain125 – 354230Capsid protein p26
PRO_0000038781
Peptide355 – 3595p1 Potential
PRO_0000272315
Chain360 – 43576Nucleocapsid protein p11
PRO_0000038782
Chain436 – 48651p9
PRO_0000038783

Regions

Zinc finger381 – 39818CCHC-type 1
Zinc finger400 – 41718CCHC-type 2
Motif457 – 4615LYPX(n)L motif

Amino acid modifications

Disulfide bond322 ↔ 342

Secondary structure

............................................................. 486
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P69732 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 97137FA5933D1DDA

FASTA48654,809
        10         20         30         40         50         60 
MGDPLTWSKA LKKLEKVTVQ GSQKLTTGNC NWALSLVDLF HDTNFVKEKD WQLRDVIPLL 

        70         80         90        100        110        120 
EDVTQTLSGQ EREAFERTWW AISAVKMGLQ INNVVDGKAS FQLLRAKYEK KTANKKQSEP 

       130        140        150        160        170        180 
SEEYPIMIDG AGNRNFRPLT PRGYTTWVNT IQTNGLLNEA SQNLFGILSV DCTSEEMNAF 

       190        200        210        220        230        240 
LDVVPGQAGQ KQILLDAIDK IADDWDNRHP LPNAPLVAPP QGPIPMTARF IRGLGVPRER 

       250        260        270        280        290        300 
QMEPAFDQFR QTYRQWIIEA MSEGIKVMIG KPKAQNIRQG AKEPYPEFVD RLLSQIKSEG 

       310        320        330        340        350        360 
HPQEISKFLT DTLTIQNANE ECRNAMRHLR PEDTLEEKMY ACRDIGTTKQ KMMLLAKALQ 

       370        380        390        400        410        420 
TGLAGPFKGG ALKGGPLKAA QTCYNCGKPG HLSSQCRAPK VCFKCKQPGH FSKQCRSVPK 

       430        440        450        460        470        480 
NGKQGAQGRP QKQTFPIQQK SQHNKSVVQE TPQTQNLYPD LSEIKKEYNV KEKDQVEDLN 


LDSLWE 

« Hide

References

[1]"Equine infectious anemia virus gag and pol genes: relatedness to visna and AIDS virus."
Stephens R.M., Casey J.W., Rice N.R.
Science 231:589-594(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Cloning, expression, purification, and characterization of the major core protein (p26) from equine infectious anemia virus."
Birkett A.J., Yelamos B., Rodriguez-Crespo I., Gavilanes F., Peterson D.L.
Biochim. Biophys. Acta 1339:62-72(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION OF P26.
[3]"Model for lentivirus capsid core assembly based on crystal dimers of EIAV p26."
Jin Z., Jin L., Peterson D.L., Lawson C.L.
J. Mol. Biol. 286:83-93(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF P26.
+Additional computationally mapped references.

Cross-references

Sequence databases

PIRFOLJEV. A03949.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EIAX-ray2.70A140-346[»]
1HEKX-ray2.80A/B1-124[»]
2BL6NMR-A381-417[»]
2EIAX-ray2.70A/B141-346[»]
2K84NMR-A457-486[»]
2R03X-ray2.59B456-463[»]
ProteinModelPortalP69732.
SMRP69732. Positions 1-109, 140-346, 381-417.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.1200.30. 1 hit.
1.10.150.90. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProIPR014834. Gag_p15.
IPR000721. Gag_p24.
IPR012344. Matrix_N_HIV/RSV.
IPR008916. Retrov_capsid_C.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
IPR001878. Znf_CCHC.
[Graphical view]
PfamPF08723. Gag_p15. 1 hit.
PF00607. Gag_p24. 1 hit.
PF00098. zf-CCHC. 2 hits.
[Graphical view]
SMARTSM00343. ZnF_C2HC. 2 hits.
[Graphical view]
SUPFAMSSF47353. SSF47353. 1 hit.
SSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEPS50158. ZF_CCHC. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP69732.

Entry information

Entry nameGAG_EIAVY
AccessionPrimary (citable) accession number: P69732
Secondary accession number(s): P03351
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: February 19, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references