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Protein

Translation repressor protein

Gene

regA

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Controls the translation of a number of proteins (such as regA itself, rIIB and at least 35 others) by binding to their mRNA.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi15 – 3723H-T-H motifSequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Translation repressor protein
Gene namesi
Name:regA
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 122122Translation repressor proteinPRO_0000164968Add
BLAST

Structurei

Secondary structure

1
122
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 54Combined sources
Helixi11 – 2010Combined sources
Beta strandi22 – 265Combined sources
Turni27 – 304Combined sources
Beta strandi31 – 344Combined sources
Beta strandi36 – 416Combined sources
Beta strandi44 – 496Combined sources
Helixi50 – 567Combined sources
Helixi65 – 8016Combined sources
Turni106 – 1083Combined sources
Helixi109 – 1113Combined sources
Beta strandi112 – 1165Combined sources
Turni117 – 1193Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1REGX-ray1.90X/Y1-122[»]
ProteinModelPortaliP69702.
SMRiP69702. Positions 1-122.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69702.

Family & Domainsi

Family and domain databases

Gene3Di3.30.70.650. 1 hit.
InterProiIPR002702. Transl_repress_RegA.
[Graphical view]
PfamiPF01818. Translat_reg. 1 hit.
[Graphical view]
ProDomiPD031547. Translat_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55064. SSF55064. 1 hit.

Sequencei

Sequence statusi: Complete.

P69702-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEITLKKPE DFLKVKETLT RMGIANNKDK VLYQSCHILQ KKGLYYIVHF
60 70 80 90 100
KEMLRMDGRQ VEMTEEDEVR RDSIAWLLED WGLIEIVPGQ RTFMKDLTNN
110 120
FRVISFKQKH EWKLVPKYTI GN
Length:122
Mass (Da):14,619
Last modified:August 13, 1987 - v1
Checksum:i616DD4E11D70EC07
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00769 Genomic DNA. Translation: CAA25343.1.
M10160 Genomic DNA. Translation: AAC05396.1.
AF158101 Genomic DNA. Translation: AAD42651.1.
PIRiA93530. RGBPT4.
RefSeqiNP_049663.1. NC_000866.4.

Genome annotation databases

GeneIDi1258694.
KEGGivg:1258694.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00769 Genomic DNA. Translation: CAA25343.1.
M10160 Genomic DNA. Translation: AAC05396.1.
AF158101 Genomic DNA. Translation: AAD42651.1.
PIRiA93530. RGBPT4.
RefSeqiNP_049663.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1REGX-ray1.90X/Y1-122[»]
ProteinModelPortaliP69702.
SMRiP69702. Positions 1-122.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258694.
KEGGivg:1258694.

Miscellaneous databases

EvolutionaryTraceiP69702.

Family and domain databases

Gene3Di3.30.70.650. 1 hit.
InterProiIPR002702. Transl_repress_RegA.
[Graphical view]
PfamiPF01818. Translat_reg. 1 hit.
[Graphical view]
ProDomiPD031547. Translat_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55064. SSF55064. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The bacteriophage T4 regA gene: primary sequence of a translational repressor."
    Trojanowska M., Miller E.S., Karam J., Stormo G., Gold L.
    Nucleic Acids Res. 12:5979-5993(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning, nucleotide sequence, and overexpression of the bacteriophage T4 regA gene."
    Adari H.Y., Rose K., Williams K.R., Konigsberg W.H., Lin T.C., Spicer E.K.
    Proc. Natl. Acad. Sci. U.S.A. 82:1901-1905(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Bacteriophage T4 regA protein binds to mRNAs and prevents translation initiation."
    Winter R.B., Morrisey L., Gauss P., Gold L., Hsu T., Karam J.
    Proc. Natl. Acad. Sci. U.S.A. 84:7822-7826(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: MECHANISM OF TRANSLATION REGULATION.
  5. "Identification of amino acid residues at the interface of a bacteriophage T4 regA protein-nucleic acid complex."
    Webster K.R., Keill S., Konigsberg W., Williams K.R., Spicer E.K.
    J. Biol. Chem. 267:26097-26103(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Crystal structure of the T4 regA translational regulator protein at 1.9-A resolution."
    Kang C., Chan R., Berger I., Lockshin C., Green L., Gold L., Rich A.
    Science 268:1170-1173(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

Entry informationi

Entry nameiREGA_BPT4
AccessioniPrimary (citable) accession number: P69702
Secondary accession number(s): P04528
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: December 9, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.