P69687 (CAPSD_TMV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Capsid protein

Alternative name(s):
Coat protein

Gene names

Name:

CP

Organism

Tobacco mosaic virus (strain vulgare) (TMV) (Tobacco mosaic virus (strain U1))

Taxonomic identifier

12243 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Virgaviridae › Tobamovirus

Virus host

Nicotiana tabacum (Common tobacco) [TaxID: 4097]

Protein attributes

Sequence length	159 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Capsid protein self-assembles to form rod-shaped virions about 18 nm in diameter with a central canal enclosing the viral genomic RNA.
Subcellular location	Virion Potential.
Sequence similarities	Belongs to the virgaviridae capsid protein family.

Ontologies

Keywords
Cellular component	Virion
Molecular function	Capsid protein
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular component	viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed; by host Ref.3 Ref.4 Ref.5

Chain

2 – 159

158

Capsid protein

PRO_0000144918

Amino acid modifications

Modified residue

2

1

N-acetylserine; by host

Natural variations

Natural variant

29

1

T → I in PM2; DT1 and DT2.

Natural variant

79

1

P → S in DT2.

Natural variant

96

1

E → D in PM2; DT1 and DT2.

Natural variant

104

1

T → M in PM2.

Natural variant

138

1

G → R in DT1 and DT2.

Natural variant

149

1

S → Y in DT2.

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

159

Helix Strand Turn

Details...

Helix

8 – 12

5

Helix

21 – 31

11

Helix

39 – 51

13

Beta strand

69 – 71

3

Helix

77 – 87

11

Helix

107 – 135

29

Turn

136 – 138

3

Helix

142 – 149

8

Sequences

	Sequence		Length	Mass (Da)
	P69687 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 5C90E8478A43DBC4	FASTA	159	17,624
	10 20 30 40 50 60 MSYSITTPSQ FVFLSSAWAD PIELINLCTN ALGNQFQTQQ ARTVVQRQFS EVWKPSPQVT 70 80 90 100 110 120 VRFPDSDFKV YRYNAVLDPL VTALLGAFDT RNRIIEVENQ ANPTTAETLD ATRRVDDATV 130 140 150 AIRSAINNLI VELIRGTGSY NRSSFESSSG LVWTSGPAT

References

[1]	"Nucleotide sequence of tobacco mosaic virus RNA." Goelet P., Lomonossoff G.P., Butler P.J.G., Akam M.E., Gait M.J., Karn J. Proc. Natl. Acad. Sci. U.S.A. 79:5818-5822(1982) [PubMed: 6964389] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Sequence of 1000 nucleotides at the 3' end of tobacco mosaic virus RNA." Guilley H., Jonard G., Kukla B., Richards K.E. Nucleic Acids Res. 6:1287-1308(1979) [PubMed: 109810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]	"The complete amino acid sequence of the protein of tobacco mosaic virus." Tsugita A., Gish D.T., Young J., Fraenkel-Conrat H., Knight C.A., Stanley W.M. Proc. Natl. Acad. Sci. U.S.A. 46:1463-1469(1960) [PubMed: 16590772] [Abstract] Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-159.
[4]	"Studies on the amino acid sequence of tobacco mosaic virus protein. V. Amino acid sequences of two peptides from tryptic digests and location of amide group." Funatsu G., Tsugita A., Fraenkel-Conrat H. Arch. Biochem. Biophys. 105:25-41(1964) [PubMed: 14165501] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-42 AND 94-113, SEQUENCE REVISION.
[5]	"The sequence of amino acids in the protein of tobacco mosaic virus. IV. Separation of tobacco mosaic virus protein with trypsin." Anderer F.A., Handschuh D. Z. Naturforsch. B 17:536-543(1962) Cited for: PROTEIN SEQUENCE OF 2-159.
[6]	"Further studies on amino acid sequence of proteins in tobacco mosaic virus." Anderer F.A., Wittmann-Liebold B., Wittmann H.G. Z. Naturforsch. B 20:1203-1213(1965) [PubMed: 4381389] [Abstract] Cited for: SEQUENCE REVISION.
[7]	"Amino acid sequences of some common Japanese strains of tobacco mosaic virus." Nozu Y., Ohno T., Okada Y. J. Biochem. 68:39-52(1970) [PubMed: 5452764] [Abstract] Cited for: SEQUENCE REVISION.
[8]	"Capsid protein gene sequences of four tobacco mosaic virus strains defective for virus assembly." Kuhlmann U., Sarkar S., Rohde W. Nucleic Acids Res. 21:3325-3325(1993) [PubMed: 8341610] [Abstract] Cited for: MUTANTS DEFECTIVE CAPSID PM2; DT1 AND DT2.
[9]	"Structure of tobacco mosaic virus at 3.6-A resolution: implications for assembly." Namba K., Stubbs G. Science 231:1401-1406(1986) [PubMed: 3952490] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.6 ANGSTROMS) OF 1-159.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

V01408 Unassigned RNA. No translation available.
V01409 Genomic RNA. No translation available.
V01406 Genomic RNA. Translation: CAA24685.1.
X70882 mRNA. Translation: CAA50227.1.
X70883 mRNA. Translation: CAA50228.1.
X70884 mRNA. Translation: CAA50229.1.

RefSeq

NP_597750.1. NC_001367.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EI7	X-ray	2.45	A/B	2-159	[»]
2TMV	fiber diffraction	2.90	P	2-159	[»]
3J06	electron microscopy	-	A	1-159	[»]
3KML	X-ray	3.01	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q	2-158	[»]

ProteinModelPortal

P69687.

SMR

P69687. Positions 2-159.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1494080.

Family and domain databases

InterPro

IPR001337. TMV-like_coat.
[Graphical view]

Pfam

PF00721. TMV_coat. 1 hit.
[Graphical view]

SUPFAM

SSF47195. TMV_coat. 1 hit.

ProtoNet

Search...

Entry information

Entry name

CAPSD_TMV

Accession

Primary (citable) accession number: P69687
Secondary accession number(s): P03570

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	September 21, 2011
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program