Reviewed, UniProtKB/Swiss-Prot P69684 (ILVB_PORUM)

Names and origin

Protein names

Recommended name:
    Acetolactate synthase large subunit
      Short name=AHAS
    EC=2.2.1.6
Alternative name(s):
    Acetohydroxy-acid synthase large subunit
      Short name=ALS

Gene names

Name:

ilvB

Encoded on

Plastid; Chloroplast

Organism

Porphyra umbilicalis (Laver)

Taxonomic identifier

2786 [NCBI]

Taxonomic lineage

Eukaryota › Rhodophyta › Bangiophyceae › Bangiales › Bangiaceae › Porphyra

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 pyruvate = 2-acetolactate + CO2.
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.
Subunit structure	Dimer of large and small chains By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Cellular component	Chloroplast Plastid
Ligand	FAD Flavoprotein Magnesium Metal-binding Thiamine pyrophosphate
Molecular function	Transferase
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro acetolactate synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW thiamin pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	Acetolactate synthase large subunit		PRO_0000090812
Regions
	Nucleotide binding	271 – 292	22	FAD By similarity
	Nucleotide binding	314 – 333	20	FAD By similarity
	Region	405 – 484	80	Thiamine pyrophosphate binding
Sites
	Metal binding	455	1	Magnesium By similarity
	Metal binding	482	1	Magnesium By similarity
	Binding site	61	1	Thiamine pyrophosphate By similarity
	Binding site	163	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P69684-1 [UniParc]. Last modified March 29, 2005. Version 1. Checksum: 1C35F7DF0F2E97AD	FASTA	590	64,930
	10 20 30 40 50 60 MLSKQIIGSE KTGRFALLDS IVRHGVIHIF GYPGGAILPI YDELYAWEEL SLIKNILVRH 70 80 90 100 110 120 EQGASHAADA YSRSTGKVGV CFATSGPGAT NLVSGIATAH IDSVPILAIT GQVGRPFIGT 130 140 150 160 170 180 DAFQEVDIFG ITLPIVKHSY VVRDPRDMSR IVAEAFYICK HGRPGPVLID VPKDVGLEKF 190 200 210 220 230 240 NYFSVEPGQV KIPGCRPLSN LKSRQILMAA KMIQQSSQPL LYIGGGAIIS DAHSIIKELV 250 260 270 280 290 300 DLYKIPVTTT LMGKGIFNED SEFCLGMLGM HGTAYANFAV SECDLLIALG ARFDDRVTGK 310 320 330 340 350 360 LDEFACNAQV IHVDIDPAEV GKNRIPQVAI VGDVTEVVTS LLNLLKNNFK PYPEQIISWQ 370 380 390 400 410 420 ERIHRWRQQY PLLVPKKSTS ISPQEILVTT NQLAQDAYFT TDVGQHQMWS AQFLKVKSKH 430 440 450 460 470 480 WISSAGLGTM GYGLPAAIGA QVAHPNELVI CVSGDSSFQM NMQELGTIAQ YKLPIKIVII 490 500 510 520 530 540 NNRWQGMVRQ WQQAFYGERY SHSRMTEGAP NFQKLAEAFG IKAFTVNNRQ NMESSLKDAM 550 560 570 580 590 KYPGPVLLDC QVTENENCYP MVAPGKSNAQ MIGIAKPQRG TASNYVSRNI

References

[1]	"Two amino-acid biosynthetic genes are encoded on the plastid genome of the red alga Porphyra umbilicalis." Reith M., Munholland J.M. Curr. Genet. 23:59-65(1993) [PubMed: 8381336] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Avonport.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M94625 Unassigned DNA. Translation: AAA03052.1.
PIR	S28920.
3D structure databases
HSSP	HSSP built from PDB template 1JSC based on UniProtKB P07342.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.2.1.6. 268420.
Family and domain databases
InterPro	IPR012846. Acetolactate_synth_lsu. IPR000399. TPP_bd_CS. IPR012001. TPP_bd_enzyme_N. IPR011766. TPP_enzyme_bd_C. IPR012000. TPP_enzyme_M. [Graphical view]
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00118. acolac_lg. 1 hit.
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVB_PORUM

Accession

Primary (citable) accession number: P69684
Secondary accession number(s): P31594

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 29, 2005
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)