Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Adaptin ear-binding coat-associated protein 1

Gene

Necap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in endocytosis.By similarity

GO - Biological processi

Keywordsi

Biological processEndocytosis, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-432722 Golgi Associated Vesicle Biogenesis
R-RNO-8856825 Cargo recognition for clathrin-mediated endocytosis
R-RNO-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Adaptin ear-binding coat-associated protein 1
Alternative name(s):
NECAP endocytosis-associated protein 1
Short name:
NECAP-1
Gene namesi
Name:Necap1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi1306053 Necap1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002130701 – 277Adaptin ear-binding coat-associated protein 1Add BLAST277

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei211PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP69682
PRIDEiP69682

PTM databases

iPTMnetiP69682
PhosphoSitePlusiP69682

Expressioni

Tissue specificityi

Expressed predominantly in brain (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000009236
GenevisibleiP69682 RN

Interactioni

Subunit structurei

Interacts with AP1G1 and AP2A1 components of the adapter protein complexes AP-1 and AP-2. Interacts with the GAE domain proteins GGA1, GGA2 and GGA3 (By similarity). Interacts with AP2A2.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AMPHP494183EBI-7592718,EBI-7121510From Homo sapiens.

Protein-protein interaction databases

BioGridi300059, 9 interactors
DIPiDIP-44064N
IntActiP69682, 5 interactors
MINTiP69682
STRINGi10116.ENSRNOP00000013272

Structurei

3D structure databases

ProteinModelPortaliP69682
SMRiP69682
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi254 – 257WXXF motif 14
Motifi274 – 277WXXF motif 24

Domaini

The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself. The WXXF motif 1, which is preceded by an acidic residue and has a glycine in second position mediates specific interaction with AP-1. The WXXF motif 2, which is followed by the C-terminal carboxyl group negative charge, allows specific interaction with AP-2.2 Publications

Sequence similaritiesi

Belongs to the NECAP family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2500 Eukaryota
ENOG41113PS LUCA
GeneTreeiENSGT00390000009359
HOGENOMiHOG000231188
HOVERGENiHBG060621
InParanoidiP69682
KOiK20069
OMAiERTDSFD
OrthoDBiEOG091G0PZV
PhylomeDBiP69682
TreeFamiTF314482

Family and domain databases

CDDicd13228 PHear_NECAP, 1 hit
Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR012466 NECAP_PHear
IPR011993 PH-like_dom_sf
PfamiView protein in Pfam
PF07933 DUF1681, 1 hit

Sequencei

Sequence statusi: Complete.

P69682-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAELEYESV LCVKPDVSVY RIPPRASNRG YRASDWKLDQ PDWTGRLRIT
60 70 80 90 100
SKGKIAYIKL EDKVSGELFA QAPVEQYPGI AVETVADSSR YFVIRIQDGT
110 120 130 140 150
GRSAFIGIGF TDRGDAFDFN VSLQDHFKWV KQETEISKES QEMDSRPKLD
160 170 180 190 200
LGFKEGQTIK LSIGNITAKK GGTSKPRASG TGGLSLLPPP PGGKVTIPPP
210 220 230 240 250
SSSVAISNHV TPPPIPKSNH GSNDSDILLD LDSPAPVPTS APAPAPASTS
260 270
NDLWGDFSTA SSSVPNQAPQ PSNWVQF
Length:277
Mass (Da):29,792
Last modified:March 29, 2005 - v1
Checksum:iF8DCDABF95C838C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03032682 Genomic DNA No translation available.
BC097496 mRNA Translation: AAH97496.1
RefSeqiNP_001025090.1, NM_001029919.2
UniGeneiRn.58356

Genome annotation databases

EnsembliENSRNOT00000013272; ENSRNOP00000013272; ENSRNOG00000009236
GeneIDi312694
KEGGirno:312694
UCSCiRGD:1306053 rat

Similar proteinsi

Entry informationi

Entry nameiNECP1_RAT
AccessioniPrimary (citable) accession number: P69682
Secondary accession number(s): Q4QR67
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: March 28, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health