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Reviewed, UniProtKB/Swiss-Prot P69681 (AMTB_ECOLI)

Last modified February 9, 2010. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Ammonia channel
Alternative name(s):
    Ammonia transporter
Gene names
Name: amtB
Synonyms: ybaG
Ordered Locus Names: b0451, JW0441
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Involved in the uptake of ammonia.

Subunit structure

Homotrimer. May interact with glnK. Ref.9

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the ammonium transporter (TC 2.A.49) family. [View classification]

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222
Chain23 – 428406Ammonia channel
PRO_0000001307

Regions

Topological domain23 – 286Periplasmic Ref.7 Ref.10
Transmembrane29 – 55271
Topological domain56 – 594Cytoplasmic Ref.7 Ref.10
Transmembrane60 – 90312
Topological domain91 – 11828Periplasmic Ref.7 Ref.10
Transmembrane119 – 141233
Topological domain142 – 1465Cytoplasmic Ref.7 Ref.10
Transmembrane147 – 170244
Topological domain171 – 18414Periplasmic Ref.7 Ref.10
Transmembrane185 – 203195
Topological domain204 – 21512Cytoplasmic Ref.7 Ref.10
Transmembrane216 – 242276
Topological domain243 – 2453Periplasmic Ref.7 Ref.10
Transmembrane246 – 275307
Topological domain276 – 2794Cytoplasmic Ref.7 Ref.10
Transmembrane280 – 294158
Topological domain295 – 3028Periplasmic Ref.7 Ref.10
Transmembrane303 – 328269
Topological domain329 – 3324Cytoplasmic Ref.7 Ref.10
Transmembrane333 – 3542210
Topological domain355 – 36915Periplasmic Ref.7 Ref.10
Transmembrane370 – 4023311
Topological domain403 – 42826Cytoplasmic Ref.7 Ref.10

Experimental info

Sequence conflict377 – 3826QLESIA → SWKASP Ref.5

Secondary structure

1 ............................................... 428
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P69681-1 [UniParc].

Last modified March 29, 2005. Version 1.
Checksum: B4AC96F0E5AE2B59

FASTA42844,515
        10         20         30         40         50         60 
MKIATIKTGL ASLAMLPGLV MAAPAVADKA DNAFMMICTA LVLFMTIPGI ALFYGGLIRG 

        70         80         90        100        110        120 
KNVLSMLTQV TVTFALVCIL WVVYGYSLAF GEGNNFFGNI NWLMLKNIEL TAVMGSIYQY 

       130        140        150        160        170        180 
IHVAFQGSFA CITVGLIVGA LAERIRFSAV LIFVVVWLTL SYIPIAHMVW GGGLLASHGA 

       190        200        210        220        230        240 
LDFAGGTVVH INAAIAGLVG AYLIGKRVGF GKEAFKPHNL PMVFTGTAIL YIGWFGFNAG 

       250        260        270        280        290        300 
SAGTANEIAA LAFVNTVVAT AAAILGWIFG EWALRGKPSL LGACSGAIAG LVGVTPACGY 

       310        320        330        340        350        360 
IGVGGALIIG VVAGLAGLWG VTMLKRLLRV DDPCDVFGVH GVCGIVGCIM TGIFAASSLG 

       370        380        390        400        410        420 
GVGFAEGVTM GHQLLVQLES IAITIVWSGV VAFIGYKLAD LTVGLRVPEE QEREGLDVNS 


HGENAYNA

« Hide

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References

« Hide 'large scale' references
[1]"An alternative PII protein in the regulation of glutamine synthetase in Escherichia coli."
van Heeswijk W.C., Hoving S., Molenaar D., Stegeman B., Kahn D., Westerhoff H.V.
Mol. Microbiol. 21:133-146(1996) [PubMed: 8843440] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Cloning, sequencing, and characterization of Escherichia coli thioesterase II."
Naggert J., Narasimhan M.L., Deveaux L., Cho H., Randhawa Z.I., Cronan J.E. Jr., Green B.N., Smith S.
J. Biol. Chem. 266:11044-11050(1991) [PubMed: 1645722] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 377-428.
Strain: K12.
[6]"Intrinsic and extrinsic approaches for detecting genes in a bacterial genome."
Borodovsky M., Rudd K.E., Koonin E.V.
Nucleic Acids Res. 22:4756-4767(1994) [PubMed: 7984428] [Abstract]
Cited for: IDENTIFICATION.
[7]"Membrane topology of the Mep/Amt family of ammonium transporters."
Thomas G.H., Mullins J.G., Merrick M.
Mol. Microbiol. 37:331-344(2000) [PubMed: 10931328] [Abstract]
Cited for: TOPOLOGY.
[8]"The glnKamtB operon. A conserved gene pair in prokaryotes."
Thomas G.H., Coutts G., Merrick M.
Trends Genet. 16:11-14(2000) [PubMed: 10637624] [Abstract]
Cited for: POSSIBLE INTERACTION WITH GLNK.
[9]"Purification of the Escherichia coli ammonium transporter AmtB reveals a trimeric stoichiometry."
Blakey D., Leech A., Thomas G.H., Coutts G., Findlay K., Merrick M.
Biochem. J. 364:527-535(2002) [PubMed: 12023896] [Abstract]
Cited for: SUBUNIT.
[10]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.
[11]"The mechanism of ammonia transport based on the crystal structure of AmtB of Escherichia coli."
Zheng L., Kostrewa D., Berneche S., Winkler F.K., Li X.-D.
Proc. Natl. Acad. Sci. U.S.A. 101:17090-17095(2004) [PubMed: 15563598] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
[12]"Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A."
Khademi S., O'Connell J.D. III, Remis J., Robles-Colmenares Y., Miercke L.J., Stroud R.M.
Science 305:1587-1594(2004) [PubMed: 15361618] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS), PROTEIN SEQUENCE OF N-TERMINUS.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U40429 Genomic DNA. Translation: AAD14837.1.
U82664 Genomic DNA. Translation: AAB40207.1.
U00096 Genomic DNA. Translation: AAC73554.1.
AP009048 Genomic DNA. Translation: BAE76231.1.
M63308 Genomic DNA. No translation available.
PIRA90692.
C64775.
E85542.
RefSeqAP_001101.1.
NP_414985.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1U77X-ray2.00A23-407[»]
1U7CX-ray1.85A23-407[»]
1U7GX-ray1.40A23-407[»]
1XQEX-ray2.10A23-428[»]
1XQFX-ray1.80A23-428[»]
2NMRX-ray2.10A23-428[»]
2NOPX-ray2.00A23-428[»]
2NOWX-ray2.20A23-428[»]
2NPCX-ray2.10A23-428[»]
2NPDX-ray2.10A23-428[»]
2NPEX-ray2.10A23-428[»]
2NPGX-ray2.00A23-428[»]
2NPJX-ray2.00A23-428[»]
2NPKX-ray2.00A23-428[»]
2NS1X-ray1.96A23-428[»]
2NUUX-ray2.50A/B/C/D/E/F25-428[»]
3C1GX-ray2.30A23-428[»]
3C1HX-ray2.20A23-428[»]
3C1IX-ray2.30A23-428[»]
3C1JX-ray2.00A23-428[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-29874N.
STRINGP69681.

Protein family/group databases

TCDB1.A.11.1.1. ammonia transporter channel (Amt) family.

Genome annotation databases

GeneID945084.
GenomeReviewsGene locus JW0441 in contig AP009048_GR.
Gene locus b0451 in contig U00096_GR.
KEGGecj:JW0441.
eco:b0451.

Organism-specific databases

EchoBASEEB1768.
EcoGeneEG11821. amtB.
CMRSearch...

Phylogenomic databases

eggNOGCOG0004.
HOGENOMHBG677626.
OMALKRWLRV.

Enzyme and pathway databases

BioCycEcoCyc:AMTB-MONOMER.
ECOL168927:B0451-MONOMER.

Gene expression databases

GenevestigatorP69681.

Family and domain databases

InterProIPR001905. Ammonium_transpt.
IPR018047. Ammonium_transpt_CS.
[Graphical view]
PANTHERPTHR11730. Ammonium_transpt. 1 hit.
PfamPF00909. Ammonium_transp. 1 hit.
[Graphical view]
TIGRFAMsTIGR00836. amt. 1 hit.
PROSITEPS01219. AMMONIUM_TRANSP. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameAMTB_ECOLI
AccessionPrimary (citable) accession number: P69681
Secondary accession number(s): P37905, Q2MBX5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: February 9, 2010
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents