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Protein

Ammonia channel

Gene

amtB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the uptake of ammonia.

GO - Molecular functioni

  • ammonium transmembrane transporter activity Source: UniProtKB
  • uniporter activity Source: EcoCyc

GO - Biological processi

  • ammonium transport Source: UniProtKB
  • carbon dioxide transport Source: UniProtKB
  • nitrogen utilization Source: GO_Central
  • organic cation transport Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Ammonia transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:AMTB-MONOMER.
ECOL316407:JW0441-MONOMER.
MetaCyc:AMTB-MONOMER.

Protein family/group databases

TCDBi1.A.11.1.1. the ammonia transporter channel (amt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ammonia channel
Alternative name(s):
Ammonia transporter
Gene namesi
Name:amtB
Synonyms:ybaG
Ordered Locus Names:b0451, JW0441
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11821. amtB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 286Periplasmic
Transmembranei29 – 5527Helical; Name=1Add
BLAST
Topological domaini56 – 594Cytoplasmic
Transmembranei60 – 9031Helical; Name=2Add
BLAST
Topological domaini91 – 11828PeriplasmicAdd
BLAST
Transmembranei119 – 14123Helical; Name=3Add
BLAST
Topological domaini142 – 1465Cytoplasmic
Transmembranei147 – 17024Helical; Name=4Add
BLAST
Topological domaini171 – 18414PeriplasmicAdd
BLAST
Transmembranei185 – 20319Helical; Name=5Add
BLAST
Topological domaini204 – 21512CytoplasmicAdd
BLAST
Transmembranei216 – 24227Helical; Name=6Add
BLAST
Topological domaini243 – 2453Periplasmic
Transmembranei246 – 27530Helical; Name=7Add
BLAST
Topological domaini276 – 2794Cytoplasmic
Transmembranei280 – 29415Helical; Name=8Add
BLAST
Topological domaini295 – 3028Periplasmic
Transmembranei303 – 32826Helical; Name=9Add
BLAST
Topological domaini329 – 3324Cytoplasmic
Transmembranei333 – 35422Helical; Name=10Add
BLAST
Topological domaini355 – 36915PeriplasmicAdd
BLAST
Transmembranei370 – 40233Helical; Name=11Add
BLAST
Topological domaini403 – 42826CytoplasmicAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 22221 PublicationAdd
BLAST
Chaini23 – 428406Ammonia channelPRO_0000001307Add
BLAST

Proteomic databases

PaxDbiP69681.
PRIDEiP69681.

Interactioni

Subunit structurei

Homotrimer. May interact with GlnK.1 Publication

Protein-protein interaction databases

BioGridi4259856. 9 interactions.
DIPiDIP-29874N.
IntActiP69681. 3 interactions.
STRINGi511145.b0451.

Structurei

Secondary structure

1
428
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi29 – 4618Combined sources
Helixi49 – 557Combined sources
Helixi60 – 8324Combined sources
Helixi85 – 906Combined sources
Beta strandi94 – 963Combined sources
Beta strandi100 – 1023Combined sources
Helixi103 – 1053Combined sources
Helixi119 – 14123Combined sources
Helixi142 – 1443Combined sources
Helixi147 – 16014Combined sources
Helixi162 – 1709Combined sources
Beta strandi171 – 1733Combined sources
Helixi174 – 1785Combined sources
Turni186 – 1894Combined sources
Helixi190 – 20314Combined sources
Turni208 – 2125Combined sources
Helixi217 – 2193Combined sources
Helixi220 – 23920Combined sources
Helixi240 – 2423Combined sources
Beta strandi243 – 2464Combined sources
Helixi247 – 27529Combined sources
Helixi280 – 29415Combined sources
Turni295 – 3006Combined sources
Helixi303 – 32826Combined sources
Helixi333 – 3353Combined sources
Helixi336 – 35419Combined sources
Helixi357 – 3593Combined sources
Helixi370 – 40233Combined sources
Helixi409 – 4146Combined sources
Helixi416 – 4216Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1U77X-ray2.00A23-407[»]
1U7CX-ray1.85A23-407[»]
1U7GX-ray1.40A23-407[»]
1XQEX-ray2.10A23-428[»]
1XQFX-ray1.80A23-428[»]
2NMRX-ray2.10A23-428[»]
2NOPX-ray2.00A23-428[»]
2NOWX-ray2.20A23-428[»]
2NPCX-ray2.10A23-428[»]
2NPDX-ray2.10A23-428[»]
2NPEX-ray2.10A23-428[»]
2NPGX-ray2.00A23-428[»]
2NPJX-ray2.00A23-428[»]
2NPKX-ray2.00A23-428[»]
2NS1X-ray1.96A23-428[»]
2NUUX-ray2.50A/B/C/D/E/F25-428[»]
3C1GX-ray2.30A23-428[»]
3C1HX-ray2.20A23-428[»]
3C1IX-ray2.30A23-428[»]
3C1JX-ray2.00A23-428[»]
ProteinModelPortaliP69681.
SMRiP69681. Positions 25-428.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69681.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000017736.
InParanoidiP69681.
KOiK03320.
OMAiCVLWVVY.
PhylomeDBiP69681.

Family and domain databases

Gene3Di1.10.3430.10. 1 hit.
InterProiIPR029020. Ammonium/urea_transptr.
IPR001905. Ammonium_transpt.
IPR018047. Ammonium_transpt_CS.
IPR024041. NH4_transpt_AmtB-like_dom.
[Graphical view]
PANTHERiPTHR11730. PTHR11730. 1 hit.
PfamiPF00909. Ammonium_transp. 1 hit.
[Graphical view]
SUPFAMiSSF111352. SSF111352. 1 hit.
TIGRFAMsiTIGR00836. amt. 1 hit.
PROSITEiPS01219. AMMONIUM_TRANSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69681-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIATIKTGL ASLAMLPGLV MAAPAVADKA DNAFMMICTA LVLFMTIPGI
60 70 80 90 100
ALFYGGLIRG KNVLSMLTQV TVTFALVCIL WVVYGYSLAF GEGNNFFGNI
110 120 130 140 150
NWLMLKNIEL TAVMGSIYQY IHVAFQGSFA CITVGLIVGA LAERIRFSAV
160 170 180 190 200
LIFVVVWLTL SYIPIAHMVW GGGLLASHGA LDFAGGTVVH INAAIAGLVG
210 220 230 240 250
AYLIGKRVGF GKEAFKPHNL PMVFTGTAIL YIGWFGFNAG SAGTANEIAA
260 270 280 290 300
LAFVNTVVAT AAAILGWIFG EWALRGKPSL LGACSGAIAG LVGVTPACGY
310 320 330 340 350
IGVGGALIIG VVAGLAGLWG VTMLKRLLRV DDPCDVFGVH GVCGIVGCIM
360 370 380 390 400
TGIFAASSLG GVGFAEGVTM GHQLLVQLES IAITIVWSGV VAFIGYKLAD
410 420
LTVGLRVPEE QEREGLDVNS HGENAYNA
Length:428
Mass (Da):44,515
Last modified:March 29, 2005 - v1
Checksum:iB4AC96F0E5AE2B59
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti377 – 3826QLESIA → SWKASP in M63308 (PubMed:1645722).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40429 Genomic DNA. Translation: AAD14837.1.
U82664 Genomic DNA. Translation: AAB40207.1.
U00096 Genomic DNA. Translation: AAC73554.1.
AP009048 Genomic DNA. Translation: BAE76231.1.
M63308 Genomic DNA. No translation available.
PIRiA90692.
C64775.
E85542.
RefSeqiNP_414985.1. NC_000913.3.
WP_000685029.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73554; AAC73554; b0451.
BAE76231; BAE76231; BAE76231.
GeneIDi945084.
KEGGiecj:JW0441.
eco:b0451.
PATRICi32116057. VBIEscCol129921_0470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40429 Genomic DNA. Translation: AAD14837.1.
U82664 Genomic DNA. Translation: AAB40207.1.
U00096 Genomic DNA. Translation: AAC73554.1.
AP009048 Genomic DNA. Translation: BAE76231.1.
M63308 Genomic DNA. No translation available.
PIRiA90692.
C64775.
E85542.
RefSeqiNP_414985.1. NC_000913.3.
WP_000685029.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1U77X-ray2.00A23-407[»]
1U7CX-ray1.85A23-407[»]
1U7GX-ray1.40A23-407[»]
1XQEX-ray2.10A23-428[»]
1XQFX-ray1.80A23-428[»]
2NMRX-ray2.10A23-428[»]
2NOPX-ray2.00A23-428[»]
2NOWX-ray2.20A23-428[»]
2NPCX-ray2.10A23-428[»]
2NPDX-ray2.10A23-428[»]
2NPEX-ray2.10A23-428[»]
2NPGX-ray2.00A23-428[»]
2NPJX-ray2.00A23-428[»]
2NPKX-ray2.00A23-428[»]
2NS1X-ray1.96A23-428[»]
2NUUX-ray2.50A/B/C/D/E/F25-428[»]
3C1GX-ray2.30A23-428[»]
3C1HX-ray2.20A23-428[»]
3C1IX-ray2.30A23-428[»]
3C1JX-ray2.00A23-428[»]
ProteinModelPortaliP69681.
SMRiP69681. Positions 25-428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259856. 9 interactions.
DIPiDIP-29874N.
IntActiP69681. 3 interactions.
STRINGi511145.b0451.

Protein family/group databases

TCDBi1.A.11.1.1. the ammonia transporter channel (amt) family.

Proteomic databases

PaxDbiP69681.
PRIDEiP69681.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73554; AAC73554; b0451.
BAE76231; BAE76231; BAE76231.
GeneIDi945084.
KEGGiecj:JW0441.
eco:b0451.
PATRICi32116057. VBIEscCol129921_0470.

Organism-specific databases

EchoBASEiEB1768.
EcoGeneiEG11821. amtB.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000017736.
InParanoidiP69681.
KOiK03320.
OMAiCVLWVVY.
PhylomeDBiP69681.

Enzyme and pathway databases

BioCyciEcoCyc:AMTB-MONOMER.
ECOL316407:JW0441-MONOMER.
MetaCyc:AMTB-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP69681.
PROiP69681.

Family and domain databases

Gene3Di1.10.3430.10. 1 hit.
InterProiIPR029020. Ammonium/urea_transptr.
IPR001905. Ammonium_transpt.
IPR018047. Ammonium_transpt_CS.
IPR024041. NH4_transpt_AmtB-like_dom.
[Graphical view]
PANTHERiPTHR11730. PTHR11730. 1 hit.
PfamiPF00909. Ammonium_transp. 1 hit.
[Graphical view]
SUPFAMiSSF111352. SSF111352. 1 hit.
TIGRFAMsiTIGR00836. amt. 1 hit.
PROSITEiPS01219. AMMONIUM_TRANSP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMTB_ECOLI
AccessioniPrimary (citable) accession number: P69681
Secondary accession number(s): P37905, Q2MBX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: September 7, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.