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Protein

Ammonia channel

Gene

amtB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the uptake of ammonia.

GO - Molecular functioni

  • ammonium transmembrane transporter activity Source: UniProtKB
  • uniporter activity Source: EcoCyc

GO - Biological processi

  • ammonium transport Source: UniProtKB
  • carbon dioxide transport Source: UniProtKB
  • nitrogen utilization Source: GO_Central
  • organic cation transport Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Ammonia transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:AMTB-MONOMER.
ECOL316407:JW0441-MONOMER.
MetaCyc:AMTB-MONOMER.

Protein family/group databases

TCDBi1.A.11.1.1. the ammonium transporter channel (amt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ammonia channel
Alternative name(s):
Ammonia transporter
Gene namesi
Name:amtB
Synonyms:ybaG
Ordered Locus Names:b0451, JW0441
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11821. amtB.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 28Periplasmic6
Transmembranei29 – 55Helical; Name=1Add BLAST27
Topological domaini56 – 59Cytoplasmic4
Transmembranei60 – 90Helical; Name=2Add BLAST31
Topological domaini91 – 118PeriplasmicAdd BLAST28
Transmembranei119 – 141Helical; Name=3Add BLAST23
Topological domaini142 – 146Cytoplasmic5
Transmembranei147 – 170Helical; Name=4Add BLAST24
Topological domaini171 – 184PeriplasmicAdd BLAST14
Transmembranei185 – 203Helical; Name=5Add BLAST19
Topological domaini204 – 215CytoplasmicAdd BLAST12
Transmembranei216 – 242Helical; Name=6Add BLAST27
Topological domaini243 – 245Periplasmic3
Transmembranei246 – 275Helical; Name=7Add BLAST30
Topological domaini276 – 279Cytoplasmic4
Transmembranei280 – 294Helical; Name=8Add BLAST15
Topological domaini295 – 302Periplasmic8
Transmembranei303 – 328Helical; Name=9Add BLAST26
Topological domaini329 – 332Cytoplasmic4
Transmembranei333 – 354Helical; Name=10Add BLAST22
Topological domaini355 – 369PeriplasmicAdd BLAST15
Transmembranei370 – 402Helical; Name=11Add BLAST33
Topological domaini403 – 428CytoplasmicAdd BLAST26

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000000130723 – 428Ammonia channelAdd BLAST406

Proteomic databases

PaxDbiP69681.
PRIDEiP69681.

Interactioni

Subunit structurei

Homotrimer. May interact with GlnK.1 Publication

Protein-protein interaction databases

BioGridi4259856. 9 interactors.
DIPiDIP-29874N.
IntActiP69681. 3 interactors.
STRINGi511145.b0451.

Structurei

Secondary structure

1428
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi29 – 46Combined sources18
Helixi49 – 55Combined sources7
Helixi60 – 83Combined sources24
Helixi85 – 90Combined sources6
Beta strandi94 – 96Combined sources3
Beta strandi100 – 102Combined sources3
Helixi103 – 105Combined sources3
Helixi119 – 141Combined sources23
Helixi142 – 144Combined sources3
Helixi147 – 160Combined sources14
Helixi162 – 170Combined sources9
Beta strandi171 – 173Combined sources3
Helixi174 – 178Combined sources5
Turni186 – 189Combined sources4
Helixi190 – 203Combined sources14
Turni208 – 212Combined sources5
Helixi217 – 219Combined sources3
Helixi220 – 239Combined sources20
Helixi240 – 242Combined sources3
Beta strandi243 – 246Combined sources4
Helixi247 – 275Combined sources29
Helixi280 – 294Combined sources15
Turni295 – 300Combined sources6
Helixi303 – 328Combined sources26
Helixi333 – 335Combined sources3
Helixi336 – 354Combined sources19
Helixi357 – 359Combined sources3
Helixi370 – 402Combined sources33
Helixi409 – 414Combined sources6
Helixi416 – 421Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U77X-ray2.00A23-407[»]
1U7CX-ray1.85A23-407[»]
1U7GX-ray1.40A23-407[»]
1XQEX-ray2.10A23-428[»]
1XQFX-ray1.80A23-428[»]
2NMRX-ray2.10A23-428[»]
2NOPX-ray2.00A23-428[»]
2NOWX-ray2.20A23-428[»]
2NPCX-ray2.10A23-428[»]
2NPDX-ray2.10A23-428[»]
2NPEX-ray2.10A23-428[»]
2NPGX-ray2.00A23-428[»]
2NPJX-ray2.00A23-428[»]
2NPKX-ray2.00A23-428[»]
2NS1X-ray1.96A23-428[»]
2NUUX-ray2.50A/B/C/D/E/F25-428[»]
3C1GX-ray2.30A23-428[»]
3C1HX-ray2.20A23-428[»]
3C1IX-ray2.30A23-428[»]
3C1JX-ray2.00A23-428[»]
ProteinModelPortaliP69681.
SMRiP69681.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69681.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000017736.
InParanoidiP69681.
KOiK03320.
OMAiCVLWVVY.
PhylomeDBiP69681.

Family and domain databases

Gene3Di1.10.3430.10. 1 hit.
InterProiIPR029020. Ammonium/urea_transptr.
IPR001905. Ammonium_transpt.
IPR018047. Ammonium_transpt_CS.
IPR024041. NH4_transpt_AmtB-like_dom.
[Graphical view]
PANTHERiPTHR11730. PTHR11730. 1 hit.
PfamiPF00909. Ammonium_transp. 1 hit.
[Graphical view]
SUPFAMiSSF111352. SSF111352. 1 hit.
TIGRFAMsiTIGR00836. amt. 1 hit.
PROSITEiPS01219. AMMONIUM_TRANSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69681-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIATIKTGL ASLAMLPGLV MAAPAVADKA DNAFMMICTA LVLFMTIPGI
60 70 80 90 100
ALFYGGLIRG KNVLSMLTQV TVTFALVCIL WVVYGYSLAF GEGNNFFGNI
110 120 130 140 150
NWLMLKNIEL TAVMGSIYQY IHVAFQGSFA CITVGLIVGA LAERIRFSAV
160 170 180 190 200
LIFVVVWLTL SYIPIAHMVW GGGLLASHGA LDFAGGTVVH INAAIAGLVG
210 220 230 240 250
AYLIGKRVGF GKEAFKPHNL PMVFTGTAIL YIGWFGFNAG SAGTANEIAA
260 270 280 290 300
LAFVNTVVAT AAAILGWIFG EWALRGKPSL LGACSGAIAG LVGVTPACGY
310 320 330 340 350
IGVGGALIIG VVAGLAGLWG VTMLKRLLRV DDPCDVFGVH GVCGIVGCIM
360 370 380 390 400
TGIFAASSLG GVGFAEGVTM GHQLLVQLES IAITIVWSGV VAFIGYKLAD
410 420
LTVGLRVPEE QEREGLDVNS HGENAYNA
Length:428
Mass (Da):44,515
Last modified:March 29, 2005 - v1
Checksum:iB4AC96F0E5AE2B59
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti377 – 382QLESIA → SWKASP in M63308 (PubMed:1645722).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40429 Genomic DNA. Translation: AAD14837.1.
U82664 Genomic DNA. Translation: AAB40207.1.
U00096 Genomic DNA. Translation: AAC73554.1.
AP009048 Genomic DNA. Translation: BAE76231.1.
M63308 Genomic DNA. No translation available.
PIRiA90692.
C64775.
E85542.
RefSeqiNP_414985.1. NC_000913.3.
WP_000685029.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73554; AAC73554; b0451.
BAE76231; BAE76231; BAE76231.
GeneIDi945084.
KEGGiecj:JW0441.
eco:b0451.
PATRICi32116057. VBIEscCol129921_0470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40429 Genomic DNA. Translation: AAD14837.1.
U82664 Genomic DNA. Translation: AAB40207.1.
U00096 Genomic DNA. Translation: AAC73554.1.
AP009048 Genomic DNA. Translation: BAE76231.1.
M63308 Genomic DNA. No translation available.
PIRiA90692.
C64775.
E85542.
RefSeqiNP_414985.1. NC_000913.3.
WP_000685029.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U77X-ray2.00A23-407[»]
1U7CX-ray1.85A23-407[»]
1U7GX-ray1.40A23-407[»]
1XQEX-ray2.10A23-428[»]
1XQFX-ray1.80A23-428[»]
2NMRX-ray2.10A23-428[»]
2NOPX-ray2.00A23-428[»]
2NOWX-ray2.20A23-428[»]
2NPCX-ray2.10A23-428[»]
2NPDX-ray2.10A23-428[»]
2NPEX-ray2.10A23-428[»]
2NPGX-ray2.00A23-428[»]
2NPJX-ray2.00A23-428[»]
2NPKX-ray2.00A23-428[»]
2NS1X-ray1.96A23-428[»]
2NUUX-ray2.50A/B/C/D/E/F25-428[»]
3C1GX-ray2.30A23-428[»]
3C1HX-ray2.20A23-428[»]
3C1IX-ray2.30A23-428[»]
3C1JX-ray2.00A23-428[»]
ProteinModelPortaliP69681.
SMRiP69681.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259856. 9 interactors.
DIPiDIP-29874N.
IntActiP69681. 3 interactors.
STRINGi511145.b0451.

Protein family/group databases

TCDBi1.A.11.1.1. the ammonium transporter channel (amt) family.

Proteomic databases

PaxDbiP69681.
PRIDEiP69681.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73554; AAC73554; b0451.
BAE76231; BAE76231; BAE76231.
GeneIDi945084.
KEGGiecj:JW0441.
eco:b0451.
PATRICi32116057. VBIEscCol129921_0470.

Organism-specific databases

EchoBASEiEB1768.
EcoGeneiEG11821. amtB.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000017736.
InParanoidiP69681.
KOiK03320.
OMAiCVLWVVY.
PhylomeDBiP69681.

Enzyme and pathway databases

BioCyciEcoCyc:AMTB-MONOMER.
ECOL316407:JW0441-MONOMER.
MetaCyc:AMTB-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP69681.
PROiP69681.

Family and domain databases

Gene3Di1.10.3430.10. 1 hit.
InterProiIPR029020. Ammonium/urea_transptr.
IPR001905. Ammonium_transpt.
IPR018047. Ammonium_transpt_CS.
IPR024041. NH4_transpt_AmtB-like_dom.
[Graphical view]
PANTHERiPTHR11730. PTHR11730. 1 hit.
PfamiPF00909. Ammonium_transp. 1 hit.
[Graphical view]
SUPFAMiSSF111352. SSF111352. 1 hit.
TIGRFAMsiTIGR00836. amt. 1 hit.
PROSITEiPS01219. AMMONIUM_TRANSP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMTB_ECOLI
AccessioniPrimary (citable) accession number: P69681
Secondary accession number(s): P37905, Q2MBX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.