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Protein

Protein ORF3

Gene

ORF3

Organism
Hepatitis E virus genotype 1 (isolate Human/Burma) (HEV-1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

May act as a viral regulatory protein involved in the modulation of mitogenic signaling pathways. May be involved in virion morphogenesis and viral pathogenesis. Expedites the processing and secretion of AMBP from the hepatocyte (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction

Names & Taxonomyi

Protein namesi
Recommended name:
Protein ORF3
Short name:
pORF3
Gene namesi
ORF Names:ORF3
OrganismiHepatitis E virus genotype 1 (isolate Human/Burma) (HEV-1)
Taxonomic identifieri31767 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageHepeviridaeHepevirus
Virus hostiBandicota bengalensis (lesser bandicoot rat) [TaxID: 69079]
Callithrix [TaxID: 9481]
Cercopithecus hamlyni (Owl-faced monkey) (Hamlyn's monkey) [TaxID: 9536]
Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops) [TaxID: 9534]
Gallus gallus (Chicken) [TaxID: 9031]
Homo sapiens (Human) [TaxID: 9606]
Macaca (macaques) [TaxID: 9539]
Mus musculus (Mouse) [TaxID: 10090]
Pan troglodytes (Chimpanzee) [TaxID: 9598]
Saimiri (squirrel monkeys) [TaxID: 9520]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000007243 Componenti: Chromosome

Subcellular locationi

  • Host cytoplasm By similarity

  • Note: The N-terminal region seems to associate with the cytoskeleton probably via one of its hydrophobic regions.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 114114Protein ORF3PRO_0000100136Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei71 – 711Phosphoserine; by hostBy similarity

Post-translational modificationi

Not glycosylated.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homodimer. Interacts with host SRC, HCK, FYN, PIK3R3 and GRB2 (via SH3 domain); binding does not activate the kinases. Interacts with host AMBP/bikunin and AMBP/alpha-1-microglobulin peptides. Interacts with host HPX/hemopexin. The phosphorylated form interacts with the unglycosylated capsid protein (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-1366939.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZZQX-ray3.81A61-114[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni6 – 2217HydrophobicAdd
BLAST
Regioni28 – 6841Interaction with host HPXBy similarityAdd
BLAST
Regioni33 – 5321HydrophobicAdd
BLAST
Regioni48 – 7225Interaction with the capsid proteinBy similarityAdd
BLAST
Regioni72 – 11443Homodimerization, and interaction with host AMBP/bikuninBy similarityAdd
BLAST
Regioni95 – 10410Interaction with host SRC, HCK, FYN, PIK3R3 and GRB2By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi6 – 2217Cys-richAdd
BLAST
Compositional biasi55 – 11258Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the hepevirus ORF3 protein family.Curated

Family and domain databases

InterProiIPR003384. HEV_Orf2.
[Graphical view]
PfamiPF02444. HEV_ORF1. 1 hit.
[Graphical view]
ProDomiPD006561. HEV_ORF2. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

P69616-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSRPCALGL FCCCSSCFCL CCPRHRPVSR LAAVVGGAAA VPAVVSGVTG
60 70 80 90 100
LILSPSQSPI FIQPTPSPPM SPLRPGLDLV FANPPDHSAP LGVTRPSAPP
110
LPHVVDLPQL GPRR
Length:114
Mass (Da):11,712
Last modified:May 20, 2008 - v2
Checksum:iC5AC40F482A1D7F6
GO

Sequence cautioni

The sequence AAA45735 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73218 Genomic RNA. Translation: AAA45735.1. Different initiation.
PIRiB40778. VHWWHE.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73218 Genomic RNA. Translation: AAA45735.1. Different initiation.
PIRiB40778. VHWWHE.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZZQX-ray3.81A61-114[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1366939.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR003384. HEV_Orf2.
[Graphical view]
PfamiPF02444. HEV_ORF1. 1 hit.
[Graphical view]
ProDomiPD006561. HEV_ORF2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiORF3_HEVBU
AccessioniPrimary (citable) accession number: P69616
Secondary accession number(s): P29325
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: May 20, 2008
Last modified: May 11, 2016
This is version 34 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.