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Protein

Nucleoprotein

Gene

N

Organism
Avian infectious bronchitis virus (strain Beaudette) (IBV)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid. Interaction with the M protein leads to the formation of virus particles. Binds to cellular membranes and phospholipids. Elicits cell-mediated immunity. May play roles in viral transcription and translation, and/or replication.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

RNA-binding, Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoprotein
Alternative name(s):
Nucleocapsid protein
Short name:
NC
Short name:
Protein N
Gene namesi
Name:N
ORF Names:6
OrganismiAvian infectious bronchitis virus (strain Beaudette) (IBV)
Taxonomic identifieri11122 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeGammacoronavirus
Virus hostiGallus gallus (Chicken) [TaxID: 9031]
Proteomesi
  • UP000006717 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host Golgi apparatus, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001059761 – 409NucleoproteinAdd BLAST409

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei190Phosphoserine; by host1 Publication1
Modified residuei192Phosphoserine; by host1 Publication1
Disulfide bondi281 ↔ 3081 Publication
Disulfide bondi320 ↔ 3231 Publication
Modified residuei378Phosphothreonine; by host1 Publication1
Modified residuei379Phosphoserine; by host1 Publication1

Post-translational modificationi

Phosphorylated on serine and threonine residues.1 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein

PTM databases

iPTMnetiP69596.

Interactioni

Subunit structurei

Monomer and oligomer. Both monomeric and oligomeric forms interact with RNA. Interacts with the M protein.

Structurei

Secondary structure

1409
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi39 – 45Combined sources7
Beta strandi51 – 53Combined sources3
Beta strandi60 – 62Combined sources3
Helixi64 – 66Combined sources3
Beta strandi68 – 79Combined sources12
Beta strandi81 – 95Combined sources15
Turni100 – 103Combined sources4
Beta strandi113 – 117Combined sources5
Turni134 – 136Combined sources3
Helixi219 – 223Combined sources5
Helixi227 – 229Combined sources3
Helixi239 – 242Combined sources4
Beta strandi247 – 249Combined sources3
Helixi256 – 261Combined sources6
Helixi262 – 264Combined sources3
Helixi266 – 272Combined sources7
Helixi278 – 284Combined sources7
Beta strandi285 – 292Combined sources8
Beta strandi295 – 306Combined sources12
Helixi313 – 323Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BTLX-ray1.95A/B29-160[»]
2BXXX-ray1.85A/B29-160[»]
2CA1X-ray2.60A/B218-326[»]
ProteinModelPortaliP69596.
SMRiP69596.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69596.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni29 – 160RNA-bindingAdd BLAST132
Regioni226 – 333DimerizationBy similarityAdd BLAST108

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi165 – 190Ser-richAdd BLAST26

Sequence similaritiesi

Family and domain databases

InterProiIPR001218. Corona_nucleocap.
[Graphical view]
PfamiPF00937. Corona_nucleoca. 1 hit.
[Graphical view]
PIRSFiPIRSF003888. Corona_nucleocap. 1 hit.

Sequencei

Sequence statusi: Complete.

P69596-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGKAAGKT DAPAPVIKLG GPKPPKVGSS GNASWFQAIK AKKLNTPPPK
60 70 80 90 100
FEGSGVPDNE NIKPSQQHGY WRRQARFKPG KGGRKPVPDA WYFYYTGTGP
110 120 130 140 150
AADLNWGDTQ DGIVWVAAKG ADTKSRSNQG TRDPDKFDQY PLRFSDGGPD
160 170 180 190 200
GNFRWDFIPL NRGRSGRSTA ASSAAASRAP SREGSRGRRS DSGDDLIARA
210 220 230 240 250
AKIIQDQQKK GSRITKAKAD EMAHRRYCKR TIPPNYRVDQ VFGPRTKGKE
260 270 280 290 300
GNFGDDKMNE EGIKDGRVTA MLNLVPSSHA CLFGSRVTPK LQLDGLHLRF
310 320 330 340 350
EFTTVVPCDD PQFDNYVKIC DQCVDGVGTR PKDDEPKPKS RSSSRPATRG
360 370 380 390 400
NSPAPRQQRP KKEKKLKKQD DEADKALTSD EERNNAQLEF YDEPKVINWG

DAALGENEL
Length:409
Mass (Da):45,032
Last modified:March 29, 2005 - v1
Checksum:i562F39C475DB8872
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti108D → Y in strain: Isolate Vero cell-adapted p65. 1
Natural varianti208Q → H in strain: Isolate Vero cell-adapted p65. 1
Natural varianti391 – 392YD → DY in strain: Isolate Vero cell-adapted p65. 2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95169 Genomic RNA. Translation: AAA70242.1.
M28565 Genomic RNA. Translation: AAA46214.1.
DQ001339 Genomic RNA. Translation: AAY24440.1.
RefSeqiNP_040838.1. NC_001451.1.

Genome annotation databases

GeneIDi1489745.
KEGGivg:1489745.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95169 Genomic RNA. Translation: AAA70242.1.
M28565 Genomic RNA. Translation: AAA46214.1.
DQ001339 Genomic RNA. Translation: AAY24440.1.
RefSeqiNP_040838.1. NC_001451.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BTLX-ray1.95A/B29-160[»]
2BXXX-ray1.85A/B29-160[»]
2CA1X-ray2.60A/B218-326[»]
ProteinModelPortaliP69596.
SMRiP69596.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiP69596.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1489745.
KEGGivg:1489745.

Miscellaneous databases

EvolutionaryTraceiP69596.

Family and domain databases

InterProiIPR001218. Corona_nucleocap.
[Graphical view]
PfamiPF00937. Corona_nucleoca. 1 hit.
[Graphical view]
PIRSFiPIRSF003888. Corona_nucleocap. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNCAP_IBVB
AccessioniPrimary (citable) accession number: P69596
Secondary accession number(s): Q4ZJS4, Q89902
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: November 30, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.