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Protein

Nucleoprotein

Gene

N

Organism
Avian infectious bronchitis virus (strain Beaudette) (IBV)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid. Interaction with the M protein leads to the formation of virus particles. Binds to cellular membranes and phospholipids. Elicits cell-mediated immunity. May play roles in viral transcription and translation, and/or replication.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

RNA-binding, Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoprotein
Alternative name(s):
Nucleocapsid protein
Short name:
NC
Short name:
Protein N
Gene namesi
Name:N
ORF Names:6
OrganismiAvian infectious bronchitis virus (strain Beaudette) (IBV)
Taxonomic identifieri11122 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeGammacoronavirus
Virus hostiGallus gallus (Chicken) [TaxID: 9031]
Proteomesi
  • UP000006717 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host Golgi apparatus, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 409409NucleoproteinPRO_0000105976Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei190 – 1901Phosphoserine; by host1 Publication
Modified residuei192 – 1921Phosphoserine; by host1 Publication
Disulfide bondi281 ↔ 3081 Publication
Disulfide bondi320 ↔ 3231 Publication
Modified residuei378 – 3781Phosphothreonine; by host1 Publication
Modified residuei379 – 3791Phosphoserine; by host1 Publication

Post-translational modificationi

Phosphorylated on serine and threonine residues.1 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein

PTM databases

iPTMnetiP69596.

Interactioni

Subunit structurei

Monomer and oligomer. Both monomeric and oligomeric forms interact with RNA. Interacts with the M protein.

Structurei

Secondary structure

1
409
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi39 – 457Combined sources
Beta strandi51 – 533Combined sources
Beta strandi60 – 623Combined sources
Helixi64 – 663Combined sources
Beta strandi68 – 7912Combined sources
Beta strandi81 – 9515Combined sources
Turni100 – 1034Combined sources
Beta strandi113 – 1175Combined sources
Turni134 – 1363Combined sources
Helixi219 – 2235Combined sources
Helixi227 – 2293Combined sources
Helixi239 – 2424Combined sources
Beta strandi247 – 2493Combined sources
Helixi256 – 2616Combined sources
Helixi262 – 2643Combined sources
Helixi266 – 2727Combined sources
Helixi278 – 2847Combined sources
Beta strandi285 – 2928Combined sources
Beta strandi295 – 30612Combined sources
Helixi313 – 32311Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BTLX-ray1.95A/B29-160[»]
2BXXX-ray1.85A/B29-160[»]
2CA1X-ray2.60A/B218-326[»]
ProteinModelPortaliP69596.
SMRiP69596. Positions 29-160, 218-325.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69596.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni29 – 160132RNA-bindingAdd
BLAST
Regioni226 – 333108DimerizationBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi165 – 19026Ser-richAdd
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR001218. Corona_nucleocap.
[Graphical view]
PfamiPF00937. Corona_nucleoca. 1 hit.
[Graphical view]
PIRSFiPIRSF003888. Corona_nucleocap. 1 hit.

Sequencei

Sequence statusi: Complete.

P69596-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGKAAGKT DAPAPVIKLG GPKPPKVGSS GNASWFQAIK AKKLNTPPPK
60 70 80 90 100
FEGSGVPDNE NIKPSQQHGY WRRQARFKPG KGGRKPVPDA WYFYYTGTGP
110 120 130 140 150
AADLNWGDTQ DGIVWVAAKG ADTKSRSNQG TRDPDKFDQY PLRFSDGGPD
160 170 180 190 200
GNFRWDFIPL NRGRSGRSTA ASSAAASRAP SREGSRGRRS DSGDDLIARA
210 220 230 240 250
AKIIQDQQKK GSRITKAKAD EMAHRRYCKR TIPPNYRVDQ VFGPRTKGKE
260 270 280 290 300
GNFGDDKMNE EGIKDGRVTA MLNLVPSSHA CLFGSRVTPK LQLDGLHLRF
310 320 330 340 350
EFTTVVPCDD PQFDNYVKIC DQCVDGVGTR PKDDEPKPKS RSSSRPATRG
360 370 380 390 400
NSPAPRQQRP KKEKKLKKQD DEADKALTSD EERNNAQLEF YDEPKVINWG

DAALGENEL
Length:409
Mass (Da):45,032
Last modified:March 29, 2005 - v1
Checksum:i562F39C475DB8872
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti108 – 1081D → Y in strain: Isolate Vero cell-adapted p65.
Natural varianti208 – 2081Q → H in strain: Isolate Vero cell-adapted p65.
Natural varianti391 – 3922YD → DY in strain: Isolate Vero cell-adapted p65.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95169 Genomic RNA. Translation: AAA70242.1.
M28565 Genomic RNA. Translation: AAA46214.1.
DQ001339 Genomic RNA. Translation: AAY24440.1.
RefSeqiNP_040838.1. NC_001451.1.

Genome annotation databases

GeneIDi1489745.
KEGGivg:1489745.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95169 Genomic RNA. Translation: AAA70242.1.
M28565 Genomic RNA. Translation: AAA46214.1.
DQ001339 Genomic RNA. Translation: AAY24440.1.
RefSeqiNP_040838.1. NC_001451.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BTLX-ray1.95A/B29-160[»]
2BXXX-ray1.85A/B29-160[»]
2CA1X-ray2.60A/B218-326[»]
ProteinModelPortaliP69596.
SMRiP69596. Positions 29-160, 218-325.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiP69596.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1489745.
KEGGivg:1489745.

Miscellaneous databases

EvolutionaryTraceiP69596.

Family and domain databases

InterProiIPR001218. Corona_nucleocap.
[Graphical view]
PfamiPF00937. Corona_nucleoca. 1 hit.
[Graphical view]
PIRSFiPIRSF003888. Corona_nucleocap. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Sequences of the nucleocapsid genes from two strains of avian infectious bronchitis virus."
    Boursnell M.E.G., Binns M.M., Foulds I.J., Brown T.D.K.
    J. Gen. Virol. 66:573-580(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus."
    Boursnell M.E.G., Brown T.D.K., Foulds I.J., Green P.F., Tomley F.M., Binns M.M.
    J. Gen. Virol. 68:57-77(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Selection of and recombination between minor variants lead to the adaptation of an avian coronavirus to primate cells."
    Fang S.G., Shen S., Tay F.P., Liu D.X.
    Biochem. Biophys. Res. Commun. 336:417-423(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Vero cell-adapted p65.
  4. "The coronavirus infectious bronchitis virus nucleoprotein localizes to the nucleolus."
    Hiscox J.A., Wurm T., Wilson L., Britton P., Cavanagh D., Brooks G.
    J. Virol. 75:506-512(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. "Localization to the nucleolus is a common feature of coronavirus nucleoproteins, and the protein may disrupt host cell division."
    Wurm T., Chen H., Hodgson T., Britton P., Brooks G., Hiscox J.A.
    J. Virol. 75:9345-9356(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. "Mass spectroscopic characterization of the coronavirus infectious bronchitis virus nucleoprotein and elucidation of the role of phosphorylation in RNA binding by using surface plasmon resonance."
    Chen H., Gill A., Dove B.K., Emmett S.R., Kemp C.F., Ritchie M.A., Dee M., Hiscox J.A.
    J. Virol. 79:1164-1179(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-190; SER-192; THR-378 AND SER-379, DISULFIDE BONDS.
  7. "The nucleocapsid protein of coronavirus infectious bronchitis virus: crystal structure of its N-terminal domain and multimerization properties."
    Fan H., Ooi A., Tan Y.W., Wang S., Fang S., Liu D.X., Lescar J.
    Structure 13:1859-1868(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 29-160.

Entry informationi

Entry nameiNCAP_IBVB
AccessioniPrimary (citable) accession number: P69596
Secondary accession number(s): Q4ZJS4, Q89902
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: May 11, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.