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Protein

DNA-Binding protein G5P

Gene

V

Organism
Enterobacteria phage f1 (Bacteriophage f1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Binds to DNA in a highly cooperative manner without pronounced sequence specificity. During synthesis of the single-stranded (progeny) viral DNA, prevents the conversion into the double-stranded replicative form. G5P is displaced by the capsid protein G8P during phage assembly on the inner bacterial membrane (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei16Involved in DNA binding1
Sitei21Involved in DNA binding1
Sitei26Involved in DNA binding1
Sitei34Involved in DNA binding1
Sitei41Involved in DNA binding, and in the dimer-dimer interactions of the protein-ssDNA complex1
Sitei46Involved in DNA binding1

GO - Molecular functioni

GO - Biological processi

  • rolling circle single-stranded viral DNA replication Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-Binding protein G5P
Short name:
G5P
Alternative name(s):
GPV
Single-stranded DNA-binding protein
Gene namesi
Name:V
OrganismiEnterobacteria phage f1 (Bacteriophage f1)
Taxonomic identifieri10863 [NCBI]
Taxonomic lineageiVirusesssDNA virusesInoviridaeInovirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000002557 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000981951 – 87DNA-Binding protein G5PAdd BLAST87

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

187
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 6Combined sources3
Helixi8 – 10Combined sources3
Beta strandi14 – 19Combined sources6
Beta strandi21 – 23Combined sources3
Beta strandi25 – 35Combined sources11
Beta strandi38 – 41Combined sources4
Beta strandi43 – 48Combined sources6
Beta strandi58 – 63Combined sources6
Helixi65 – 67Combined sources3
Beta strandi68 – 70Combined sources3
Beta strandi74 – 80Combined sources7
Beta strandi83 – 85Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AE2X-ray2.00A1-87[»]
1AE3X-ray2.00A1-86[»]
1GKHX-ray1.70A1-87[»]
1GPVmodel-A/B1-87[»]
1GVPX-ray1.60A1-87[»]
1VQAX-ray1.80A1-87[»]
1VQBX-ray1.80A1-87[»]
1VQCX-ray1.80A1-87[»]
1VQDX-ray1.82A1-87[»]
1VQEX-ray1.80A1-87[»]
1VQFX-ray1.80A1-87[»]
1VQGX-ray1.82A1-87[»]
1VQHX-ray1.80A1-87[»]
1VQIX-ray1.80A1-87[»]
1VQJX-ray1.80A1-87[»]
1YHAX-ray2.50A/B1-87[»]
1YHBX-ray2.20A1-87[»]
ProteinModelPortaliP69543.
SMRiP69543.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69543.

Family & Domainsi

Sequence similaritiesi

Belongs to the inovirus G5P protein family.Curated

Family and domain databases

InterProiIPR012340. NA-bd_OB-fold.
IPR003512. Phage_M13_G5P_DNA-bd.
[Graphical view]
PfamiPF02303. Phage_DNA_bind. 1 hit.
[Graphical view]
ProDomiPD015272. Phage_DNA_bind. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50249. SSF50249. 1 hit.

Sequencei

Sequence statusi: Complete.

P69543-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKVEIKPSQ AQFTTRSGVS RQGKPYSLNE QLCYVDLGNE YPVLVKITLD
60 70 80
EGQPAYAPGL YTVHLSSFKV GQFGSLMIDR LRLVPAK
Length:87
Mass (Da):9,688
Last modified:July 21, 1986 - v1
Checksum:i2FAD178DDC248CF0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00606 Genomic DNA. Translation: CAA23868.1.
J02448 Genomic DNA. Translation: AAA32211.1.
M10677 Genomic DNA. Translation: AAA32226.1.
PIRiE04271. DDBPF1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00606 Genomic DNA. Translation: CAA23868.1.
J02448 Genomic DNA. Translation: AAA32211.1.
M10677 Genomic DNA. Translation: AAA32226.1.
PIRiE04271. DDBPF1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AE2X-ray2.00A1-87[»]
1AE3X-ray2.00A1-86[»]
1GKHX-ray1.70A1-87[»]
1GPVmodel-A/B1-87[»]
1GVPX-ray1.60A1-87[»]
1VQAX-ray1.80A1-87[»]
1VQBX-ray1.80A1-87[»]
1VQCX-ray1.80A1-87[»]
1VQDX-ray1.82A1-87[»]
1VQEX-ray1.80A1-87[»]
1VQFX-ray1.80A1-87[»]
1VQGX-ray1.82A1-87[»]
1VQHX-ray1.80A1-87[»]
1VQIX-ray1.80A1-87[»]
1VQJX-ray1.80A1-87[»]
1YHAX-ray2.50A/B1-87[»]
1YHBX-ray2.20A1-87[»]
ProteinModelPortaliP69543.
SMRiP69543.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP69543.

Family and domain databases

InterProiIPR012340. NA-bd_OB-fold.
IPR003512. Phage_M13_G5P_DNA-bd.
[Graphical view]
PfamiPF02303. Phage_DNA_bind. 1 hit.
[Graphical view]
ProDomiPD015272. Phage_DNA_bind. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50249. SSF50249. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiG5P_BPF1
AccessioniPrimary (citable) accession number: P69543
Secondary accession number(s): P03669
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.