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Protein

Capsid protein G8P

Gene

VIII

Organism
Enterobacteria phage fd (Bacteriophage fd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity).By similarity

Names & Taxonomyi

Protein namesi
Recommended name:
Capsid protein G8P
Alternative name(s):
Coat protein B
Gene 8 protein
Short name:
G8P
Major coat protein
Gene namesi
Name:VIII
OrganismiEnterobacteria phage fd (Bacteriophage fd)
Taxonomic identifieri10864 [NCBI]
Taxonomic lineageiVirusesssDNA virusesInoviridaeInovirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000001836 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 4421PeriplasmicAdd
BLAST
Transmembranei45 – 6521HelicalBy similarityAdd
BLAST
Topological domaini66 – 738Cytoplasmic

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23232 PublicationsAdd
BLAST
Chaini24 – 7350Capsid protein G8PPRO_0000003296Add
BLAST

Interactioni

Subunit structurei

Homomultimerizes. There are several thousands of this protein in the phage capsid (By similarity).By similarity

Structurei

Secondary structure

1
73
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi28 – 7043Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FDMNMR-A24-73[»]
1IFDfiber diffraction4.00A24-73[»]
1IFIfiber diffraction3.30A24-73[»]
1IFJfiber diffraction3.30A24-73[»]
1MZTNMR-A24-73[»]
2C0WX-ray3.20A24-73[»]
2C0XNMR-A24-73[»]
2HI5electron microscopy8.00A24-73[»]
ProteinModelPortaliP69539.
SMRiP69539. Positions 24-73.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69539.

Family & Domainsi

Sequence similaritiesi

Belongs to the inovirus capsid protein family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.20.5.80. 1 hit.
InterProiIPR008020. G8P.
IPR023390. Phage_M13_G8P_capsid-dom.
[Graphical view]
PfamiPF05371. Phage_Coat_Gp8. 1 hit.
[Graphical view]
PIRSFiPIRSF004117. Phage_coat_B. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69539-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKSLVLKAS VAVATLVPML SFAAEGDDPA KAAFDSLQAS ATEYIGYAWA
60 70
MVVVIVGATI GIKLFKKFTS KAS
Length:73
Mass (Da):7,626
Last modified:July 21, 1986 - v1
Checksum:i53ACF68DB18E6A52
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02451 Genomic DNA. Translation: AAA32308.1.
PIRiA04226. VCBPFD.
RefSeqiYP_009111302.1. NC_025824.1.

Genome annotation databases

GeneIDi22475003.
KEGGivg:22475003.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02451 Genomic DNA. Translation: AAA32308.1.
PIRiA04226. VCBPFD.
RefSeqiYP_009111302.1. NC_025824.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FDMNMR-A24-73[»]
1IFDfiber diffraction4.00A24-73[»]
1IFIfiber diffraction3.30A24-73[»]
1IFJfiber diffraction3.30A24-73[»]
1MZTNMR-A24-73[»]
2C0WX-ray3.20A24-73[»]
2C0XNMR-A24-73[»]
2HI5electron microscopy8.00A24-73[»]
ProteinModelPortaliP69539.
SMRiP69539. Positions 24-73.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi22475003.
KEGGivg:22475003.

Miscellaneous databases

EvolutionaryTraceiP69539.

Family and domain databases

Gene3Di1.20.5.80. 1 hit.
InterProiIPR008020. G8P.
IPR023390. Phage_M13_G8P_capsid-dom.
[Graphical view]
PfamiPF05371. Phage_Coat_Gp8. 1 hit.
[Graphical view]
PIRSFiPIRSF004117. Phage_coat_B. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 478 / Heidelberg.
  2. "Virus proteins. IV. Constitution of the coat protein of the fd phage."
    Asbeck F., Beyreuther K., Kohler H., von Wettstein G., Braunitzer G.
    Hoppe-Seyler's Z. Physiol. Chem. 350:1047-1066(1969) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-73.
    Strain: 478 / Heidelberg.
  3. "Reinvestigation of a region of the fd bacteriophage coat protein sequence."
    Nakashima Y., Konigsberg W.
    J. Mol. Biol. 88:598-600(1974) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-73, SEQUENCE REVISION.
  4. "fd coat protein structure in membrane environments: structural dynamics of the loop between the hydrophobic trans-membrane helix and the amphipathic in-plane helix."
    Almeida F.C., Opella S.J.
    J. Mol. Biol. 270:481-495(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
  5. "Molecular models and structural comparisons of native and mutant class I filamentous bacteriophages Ff (fd, f1, M13), If1 and IKe."
    Marvin D.A., Hale R.D., Nave C., Citterich M.H.
    J. Mol. Biol. 235:260-286(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS).

Entry informationi

Entry nameiCAPSD_BPFD
AccessioniPrimary (citable) accession number: P69539
Secondary accession number(s): P03617
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: December 9, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.