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Protein

Aminopeptidase O

Gene

Aopep

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Aminopeptidases catalyze the hydrolysis of amino acid residues from the N-terminus of peptide or protein substrates. Able to cleave angiotensin III to generate angiotensin IV, a bioactive peptide of the renin-angiotensin pathway. Not able to cleave angiotensin I and angiotensin II. May play a role in the proteolytic processing of bioactive peptides in tissues such as testis and heart (By similarity).By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Strongly inhibited by ophenanthroline, a metalloprotease inhibitor. Inhibited by arphamenine A, a potent inhibitor of aminopeptidases. Not affected by AEBSF and E-64, which are common inhibitors of serine- and cysteine-proteases, respectively (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi478Zinc; catalyticBy similarity1
Active sitei479Proton acceptorBy similarity1
Metal bindingi482Zinc; catalyticBy similarity1
Metal bindingi501Zinc; catalyticBy similarity1
Sitei585Transition state stabilizerBy similarity1

GO - Molecular functioni

  • aminopeptidase activity Source: UniProtKB
  • metalloaminopeptidase activity Source: GO_Central
  • metallopeptidase activity Source: UniProtKB
  • peptide binding Source: GO_Central
  • zinc ion binding Source: GO_Central

GO - Biological processi

  • peptide catabolic process Source: GO_Central
  • proteolysis Source: UniProtKB

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM01.028

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase O (EC:3.4.11.-)
Short name:
AP-O
Gene namesi
Name:Aopep
Synonyms:Onpep
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1309592 RGD1309592

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000950931 – 816Aminopeptidase OAdd BLAST816

Proteomic databases

PaxDbiP69527
PRIDEiP69527

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023638

Structurei

3D structure databases

ProteinModelPortaliP69527
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Phylogenomic databases

eggNOGiKOG1047 Eukaryota
COG0308 LUCA
HOGENOMiHOG000033924
HOVERGENiHBG050496
InParanoidiP69527
KOiK09606
PhylomeDBiP69527

Family and domain databases

Gene3Di1.25.40.320, 1 hit
InterProiView protein in InterPro
IPR033577 AOPep
IPR016024 ARM-type_fold
IPR038502 M1_LTA-4_hydro/amino_C_sf
IPR001930 Peptidase_M1
IPR015211 Peptidase_M1_C
IPR014782 Peptidase_M1_N
PANTHERiPTHR11533 PTHR11533, 1 hit
PTHR11533:SF175 PTHR11533:SF175, 1 hit
PfamiView protein in Pfam
PF09127 Leuk-A4-hydro_C, 1 hit
PF01433 Peptidase_M1, 1 hit
SMARTiView protein in SMART
SM01263 Leuk-A4-hydro_C, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit

Sequencei

Sequence statusi: Complete.

P69527-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDINLDPSRD DLPLMANTSH LLVKHYVLDL DVDFGSRVLE GNIVLFFGDG
60 70 80 90 100
NRCKKQSSSS QETFQMESEE AYILRTAEPC HVPKMDSNTF SPKMGHREFA
110 120 130 140 150
VFGKSDQDAF DNDGNHDNKE HDSESSSSKY CCDTGNHGRE DFLLVLDCCD
160 170 180 190 200
LSVLKVEEVD VAAVPDLEKF TKAPNLTAAP EKRRCEIVRD LVALPADAWR
210 220 230 240 250
EQLDCYTRCS QAPGCGELLF DSDKWSLQIR KKGVPTAADF PHAIRIWYKT
260 270 280 290 300
KPEGQSVTWT SDQDGRPCVY TMGSPINNRA LFPCQEPPVA MSTWQATVRA
310 320 330 340 350
AASFVVLMSG EKSAKPTPLR ESWHYYVTMP MPASTFTIAV GCWTEMKPKT
360 370 380 390 400
SPLDDLTEHT LPLRPSDADF RFVFGNCSHM EYPCRFQSAS AATQNIIPYR
410 420 430 440 450
VFAPVCLEGA CREALLWLIP SCLSAAHSVL GTHPFSRLDI LIVPTNFPSL
460 470 480 490 500
GMASPHIIFL SQSTLTGTSH LCGTRLCHEI AHAWFGLAIG ARDWTEEWLS
510 520 530 540 550
EGFATHLEDI FWAEAQQLPP HEALEQQELR ACLRWHRLQD ELQNSPEGMQ
560 570 580 590 600
VLRPNKEKTG HVSASGASVV KNGLNPEKGF MQVHYLKGYF LLRFLARTLG
610 620 630 640 650
EETYFPFLRK FVHLFHGQLI LSQDFLQMLL ESIPENKRFG LSVENIVGDW
660 670 680 690 700
LECPGIPKAL QEERKAKDCS PSRLVRQVAK WIRVNRRPGK RQRRKREAAF
710 720 730 740 750
EKLSPDQIVL LLEWLLEQKT LSPQTLHRLQ QTYHLQEQDA EVRHRWCELV
760 770 780 790 800
IKHKYTKAYD QVKRFLQEDQ AMGIYLYGEL MVSEDARLQQ LAHRCFELVK
810
GHMDKASAQV VTEMLF
Length:816
Mass (Da):92,837
Last modified:March 15, 2005 - v1
Checksum:i6573B2F8C258CD81
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ810421 mRNA Translation: CAH17903.1
RefSeqiNP_001012346.1, NM_001012346.1
UniGeneiRn.229038
Rn.263

Genome annotation databases

GeneIDi290963
KEGGirno:290963
UCSCiRGD:1309592 rat

Similar proteinsi

Entry informationi

Entry nameiAMPO_RAT
AccessioniPrimary (citable) accession number: P69527
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: May 23, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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