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Protein

Aminopeptidase O

Gene

Aopep

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Aminopeptidases catalyze the hydrolysis of amino acid residues from the N-terminus of peptide or protein substrates. Able to cleave angiotensin III to generate angiotensin IV, a bioactive peptide of the renin-angiotensin pathway. Not able to cleave angiotensin I and angiotensin II. May play a role in the proteolytic processing of bioactive peptides in tissues such as testis and heart (By similarity).By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Strongly inhibited by ophenanthroline, a metalloprotease inhibitor. Inhibited by arphamenine A, a potent inhibitor of aminopeptidases. Not affected by AEBSF and E-64, which are common inhibitors of serine- and cysteine-proteases, respectively (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi478 – 4781Zinc; catalyticBy similarity
Active sitei479 – 4791Proton acceptorBy similarity
Metal bindingi482 – 4821Zinc; catalyticBy similarity
Metal bindingi501 – 5011Zinc; catalyticBy similarity
Sitei585 – 5851Transition state stabilizerBy similarity

GO - Molecular functioni

  • aminopeptidase activity Source: UniProtKB
  • metallopeptidase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • leukotriene biosynthetic process Source: InterPro
  • proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM01.028.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase O (EC:3.4.11.-)
Short name:
AP-O
Gene namesi
Name:Aopep
Synonyms:Onpep
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1309592. RGD1309592.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 816816Aminopeptidase OPRO_0000095093Add
BLAST

Proteomic databases

PaxDbiP69527.
PRIDEiP69527.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023638.

Structurei

3D structure databases

ProteinModelPortaliP69527.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Phylogenomic databases

eggNOGiKOG1047. Eukaryota.
COG0308. LUCA.
HOGENOMiHOG000033924.
HOVERGENiHBG050496.
InParanoidiP69527.
KOiK09606.
PhylomeDBiP69527.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR001930. Peptidase_M1.
IPR015211. Peptidase_M1_C.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 4 hits.
PfamiPF09127. Leuk-A4-hydro_C. 1 hit.
PF01433. Peptidase_M1. 2 hits.
[Graphical view]
SMARTiSM01263. Leuk-A4-hydro_C. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

P69527-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDINLDPSRD DLPLMANTSH LLVKHYVLDL DVDFGSRVLE GNIVLFFGDG
60 70 80 90 100
NRCKKQSSSS QETFQMESEE AYILRTAEPC HVPKMDSNTF SPKMGHREFA
110 120 130 140 150
VFGKSDQDAF DNDGNHDNKE HDSESSSSKY CCDTGNHGRE DFLLVLDCCD
160 170 180 190 200
LSVLKVEEVD VAAVPDLEKF TKAPNLTAAP EKRRCEIVRD LVALPADAWR
210 220 230 240 250
EQLDCYTRCS QAPGCGELLF DSDKWSLQIR KKGVPTAADF PHAIRIWYKT
260 270 280 290 300
KPEGQSVTWT SDQDGRPCVY TMGSPINNRA LFPCQEPPVA MSTWQATVRA
310 320 330 340 350
AASFVVLMSG EKSAKPTPLR ESWHYYVTMP MPASTFTIAV GCWTEMKPKT
360 370 380 390 400
SPLDDLTEHT LPLRPSDADF RFVFGNCSHM EYPCRFQSAS AATQNIIPYR
410 420 430 440 450
VFAPVCLEGA CREALLWLIP SCLSAAHSVL GTHPFSRLDI LIVPTNFPSL
460 470 480 490 500
GMASPHIIFL SQSTLTGTSH LCGTRLCHEI AHAWFGLAIG ARDWTEEWLS
510 520 530 540 550
EGFATHLEDI FWAEAQQLPP HEALEQQELR ACLRWHRLQD ELQNSPEGMQ
560 570 580 590 600
VLRPNKEKTG HVSASGASVV KNGLNPEKGF MQVHYLKGYF LLRFLARTLG
610 620 630 640 650
EETYFPFLRK FVHLFHGQLI LSQDFLQMLL ESIPENKRFG LSVENIVGDW
660 670 680 690 700
LECPGIPKAL QEERKAKDCS PSRLVRQVAK WIRVNRRPGK RQRRKREAAF
710 720 730 740 750
EKLSPDQIVL LLEWLLEQKT LSPQTLHRLQ QTYHLQEQDA EVRHRWCELV
760 770 780 790 800
IKHKYTKAYD QVKRFLQEDQ AMGIYLYGEL MVSEDARLQQ LAHRCFELVK
810
GHMDKASAQV VTEMLF
Length:816
Mass (Da):92,837
Last modified:March 15, 2005 - v1
Checksum:i6573B2F8C258CD81
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ810421 mRNA. Translation: CAH17903.1.
RefSeqiNP_001012346.1. NM_001012346.1.
UniGeneiRn.229038.

Genome annotation databases

GeneIDi290963.
KEGGirno:290963.
UCSCiRGD:1309592. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ810421 mRNA. Translation: CAH17903.1.
RefSeqiNP_001012346.1. NM_001012346.1.
UniGeneiRn.229038.

3D structure databases

ProteinModelPortaliP69527.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023638.

Protein family/group databases

MEROPSiM01.028.

Proteomic databases

PaxDbiP69527.
PRIDEiP69527.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi290963.
KEGGirno:290963.
UCSCiRGD:1309592. rat.

Organism-specific databases

CTDi290963.
RGDi1309592. RGD1309592.

Phylogenomic databases

eggNOGiKOG1047. Eukaryota.
COG0308. LUCA.
HOGENOMiHOG000033924.
HOVERGENiHBG050496.
InParanoidiP69527.
KOiK09606.
PhylomeDBiP69527.

Miscellaneous databases

NextBioi631897.
PROiP69527.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR001930. Peptidase_M1.
IPR015211. Peptidase_M1_C.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 4 hits.
PfamiPF09127. Leuk-A4-hydro_C. 1 hit.
PF01433. Peptidase_M1. 2 hits.
[Graphical view]
SMARTiSM01263. Leuk-A4-hydro_C. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Identification of human aminopeptidase O, a novel metalloprotease with structural similarity to aminopeptidase B and leukotriene A4 hydrolase."
    Diaz-Perales A., Quesada V., Sanchez L.M., Ugalde A.P., Suarez M.F., Fueyo A., Lopez-Otin C.
    J. Biol. Chem. 280:14310-14317(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Lung.

Entry informationi

Entry nameiAMPO_RAT
AccessioniPrimary (citable) accession number: P69527
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: May 11, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.