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Protein

Iron-sulfur cluster repair protein YtfE

Gene

ytfE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Di-iron-containing protein involved in the repair of iron-sulfur clusters damaged by oxidative and nitrosative stress conditions.3 Publications

GO - Molecular functioni

GO - Biological processi

  • protein repair Source: EcoCyc
  • response to stress Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

Stress response

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7866-MONOMER.
ECOL316407:JW4167-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-sulfur cluster repair protein YtfE
Alternative name(s):
Regulator of cell morphogenesis and NO signaling
Short name:
RCMNS
Gene namesi
Name:ytfE
Ordered Locus Names:b4209, JW4167
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12505. ytfE.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mutants show a severe growth impairment under nitrosative and oxidative stress conditions. Mutation increases the sensitivity of the bacterium to iron starvation, increases the intracellular levels of free iron, and decreases the activity of several iron-sulfur-containing proteins.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220Iron-sulfur cluster repair protein YtfEPRO_0000213049Add
BLAST

Proteomic databases

EPDiP69506.
PaxDbiP69506.
PRIDEiP69506.

Expressioni

Inductioni

Induced by nitric oxide. Negatively regulated by Fnr and Fur, but no obvious Fnr or Fur binding sites were found, suggesting that this regulation could be indirect.2 Publications

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4263431. 77 interactions.
DIPiDIP-35833N.
IntActiP69506. 1 interaction.
STRINGi511145.b4209.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5FNNX-ray2.09A/B1-220[»]
5FNPX-ray1.80A/B1-220[»]
5FNYX-ray2.01A/B1-220[»]
ProteinModelPortaliP69506.
SMRiP69506. Positions 2-60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RIC family. YtfE subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105FJH. Bacteria.
COG2846. LUCA.
HOGENOMiHOG000286324.
InParanoidiP69506.
KOiK07322.
OMAiACTTWRV.
OrthoDBiEOG68DD4M.
PhylomeDBiP69506.

Family and domain databases

HAMAPiMF_01606. RIC_YtfE.
InterProiIPR023742. FeS-repair_YftE.
IPR012312. Haemerythrin-like.
IPR019903. RIC_family.
[Graphical view]
PfamiPF01814. Hemerythrin. 1 hit.
PF04405. ScdA_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03652. FeS_repair_RIC. 1 hit.

Sequencei

Sequence statusi: Complete.

P69506-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYRDQPLGE LALSIPRASA LFRKYDMDYC CGGKQTLARA AARKELDVEV
60 70 80 90 100
IEAELAKLAE QPIEKDWRSA PLAEIIDHII VRYHDRHREQ LPELILQATK
110 120 130 140 150
VERVHADKPS VPKGLTKYLT MLHEELSSHM MKEEQILFPM IKQGMGSQAM
160 170 180 190 200
GPISVMESEH DEAGELLEVI KHTTNNVTPP PEACTTWKAM YNGINELIDD
210 220
LMDHISLENN VLFPRALAGE
Length:220
Mass (Da):24,883
Last modified:March 15, 2005 - v1
Checksum:iCC81A560EDA6A03B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97105.1.
U00096 Genomic DNA. Translation: AAC77166.1.
AP009048 Genomic DNA. Translation: BAE78210.1.
PIRiS56434.
RefSeqiNP_418630.1. NC_000913.3.
WP_000331456.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77166; AAC77166; b4209.
BAE78210; BAE78210; BAE78210.
GeneIDi948724.
KEGGiecj:JW4167.
eco:b4209.
PATRICi32123993. VBIEscCol129921_4341.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97105.1.
U00096 Genomic DNA. Translation: AAC77166.1.
AP009048 Genomic DNA. Translation: BAE78210.1.
PIRiS56434.
RefSeqiNP_418630.1. NC_000913.3.
WP_000331456.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5FNNX-ray2.09A/B1-220[»]
5FNPX-ray1.80A/B1-220[»]
5FNYX-ray2.01A/B1-220[»]
ProteinModelPortaliP69506.
SMRiP69506. Positions 2-60.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263431. 77 interactions.
DIPiDIP-35833N.
IntActiP69506. 1 interaction.
STRINGi511145.b4209.

Proteomic databases

EPDiP69506.
PaxDbiP69506.
PRIDEiP69506.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77166; AAC77166; b4209.
BAE78210; BAE78210; BAE78210.
GeneIDi948724.
KEGGiecj:JW4167.
eco:b4209.
PATRICi32123993. VBIEscCol129921_4341.

Organism-specific databases

EchoBASEiEB2398.
EcoGeneiEG12505. ytfE.

Phylogenomic databases

eggNOGiENOG4105FJH. Bacteria.
COG2846. LUCA.
HOGENOMiHOG000286324.
InParanoidiP69506.
KOiK07322.
OMAiACTTWRV.
OrthoDBiEOG68DD4M.
PhylomeDBiP69506.

Enzyme and pathway databases

BioCyciEcoCyc:G7866-MONOMER.
ECOL316407:JW4167-MONOMER.

Miscellaneous databases

PROiP69506.

Family and domain databases

HAMAPiMF_01606. RIC_YtfE.
InterProiIPR023742. FeS-repair_YftE.
IPR012312. Haemerythrin-like.
IPR019903. RIC_family.
[Graphical view]
PfamiPF01814. Hemerythrin. 1 hit.
PF04405. ScdA_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03652. FeS_repair_RIC. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "New genes implicated in the protection of anaerobically grown Escherichia coli against nitric oxide."
    Justino M.C., Vicente J.B., Teixeira M., Saraiva L.M.
    J. Biol. Chem. 280:2636-2643(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, DISRUPTION PHENOTYPE.
    Strain: K12 / ATCC 23716 / DSM 498.
  5. "Escherichia coli YtfE is a di-iron protein with an important function in assembly of iron-sulphur clusters."
    Justino M.C., Almeida C.C., Goncalves V.L., Teixeira M., Saraiva L.M.
    FEMS Microbiol. Lett. 257:278-284(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, DISRUPTION PHENOTYPE, IRON-BINDING.
    Strain: K12 / ATCC 23716 / DSM 498.
  6. "Escherichia coli di-iron YtfE protein is necessary for the repair of stress-damaged iron-sulfur clusters."
    Justino M.C., Almeida C.C., Teixeira M., Saraiva L.M.
    J. Biol. Chem. 282:10352-10359(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: K12 / ATCC 23716 / DSM 498.
  7. "Iron-sulfur repair YtfE protein from Escherichia coli: structural characterization of the di-iron center."
    Todorovic S., Justino M.C., Wellenreuther G., Hildebrandt P., Murgida D.H., Meyer-Klaucke W., Saraiva L.M.
    J. Biol. Inorg. Chem. 13:765-770(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, IRON-BINDING.

Entry informationi

Entry nameiYTFE_ECOLI
AccessioniPrimary (citable) accession number: P69506
Secondary accession number(s): P39313, Q2M696
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: June 8, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.