P69506 (YTFE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Iron-sulfur cluster repair protein YtfE

Alternative name(s):
Regulator of cell morphogenesis and NO signaling
Short name=RCMNS

Gene names

Name:	ytfE
Ordered Locus Names:	b4209, JW4167

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Di-iron-containing protein involved in the repair of iron-sulfur clusters damaged by oxidative and nitrosative stress conditions. Ref.5 Ref.6 Ref.7
Subunit structure	Homodimer. Ref.7
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01606.
Induction	Induced by nitric oxide. Negatively regulated by Fnr and Fur, but no obvious Fnr or Fur binding sites were found, suggesting that this regulation could be indirect. Ref.4 Ref.5
Disruption phenotype	Mutants show a severe growth impairment under nitrosative and oxidative stress conditions. Mutation increases the sensitivity of the bacterium to iron starvation, increases the intracellular levels of free iron, and decreases the activity of several iron-sulfur-containing proteins. Ref.4 Ref.5 Ref.6
Sequence similarities	Belongs to the RIC family. YtfE subfamily.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein repair Inferred from direct assay Ref.6. Source: EcoCyc response to stress Inferred from mutant phenotype Ref.4. Source: EcoCyc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Iron-sulfur cluster repair protein YtfE HAMAP-Rule MF_01606		PRO_0000213049

Sequences

	Sequence		Length	Mass (Da)
	P69506 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: CC81A560EDA6A03B	FASTA	220	24,883
	10 20 30 40 50 60 MAYRDQPLGE LALSIPRASA LFRKYDMDYC CGGKQTLARA AARKELDVEV IEAELAKLAE 70 80 90 100 110 120 QPIEKDWRSA PLAEIIDHII VRYHDRHREQ LPELILQATK VERVHADKPS VPKGLTKYLT 130 140 150 160 170 180 MLHEELSSHM MKEEQILFPM IKQGMGSQAM GPISVMESEH DEAGELLEVI KHTTNNVTPP 190 200 210 220 PEACTTWKAM YNGINELIDD LMDHISLENN VLFPRALAGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"New genes implicated in the protection of anaerobically grown Escherichia coli against nitric oxide." Justino M.C., Vicente J.B., Teixeira M., Saraiva L.M. J. Biol. Chem. 280:2636-2643(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION, DISRUPTION PHENOTYPE. Strain: K12 / ATCC 23716 / DSM 498.
[5]	"Escherichia coli YtfE is a di-iron protein with an important function in assembly of iron-sulphur clusters." Justino M.C., Almeida C.C., Goncalves V.L., Teixeira M., Saraiva L.M. FEMS Microbiol. Lett. 257:278-284(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION, DISRUPTION PHENOTYPE, IRON-BINDING. Strain: K12 / ATCC 23716 / DSM 498.
[6]	"Escherichia coli di-iron YtfE protein is necessary for the repair of stress-damaged iron-sulfur clusters." Justino M.C., Almeida C.C., Teixeira M., Saraiva L.M. J. Biol. Chem. 282:10352-10359(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE. Strain: K12 / ATCC 23716 / DSM 498.
[7]	"Iron-sulfur repair YtfE protein from Escherichia coli: structural characterization of the di-iron center." Todorovic S., Justino M.C., Wellenreuther G., Hildebrandt P., Murgida D.H., Meyer-Klaucke W., Saraiva L.M. J. Biol. Inorg. Chem. 13:765-770(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, IRON-BINDING.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U14003 Genomic DNA. Translation: AAA97105.1. U00096 Genomic DNA. Translation: AAC77166.1. AP009048 Genomic DNA. Translation: BAE78210.1.
PIR	S56434.
RefSeq	NP_418630.1. NC_000913.2. YP_492351.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P69506.
SMR	P69506. Positions 2-62.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-35833N.
IntAct	P69506. 1 interaction.
STRING	511145.b4209.
Proteomic databases
PaxDb	P69506.
PRIDE	P69506.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC77166; AAC77166; b4209. BAE78210; BAE78210; BAE78210.
GeneID	12934437. 948724.
KEGG	ecj:Y75_p4095. eco:b4209.
PATRIC	32123993. VBIEscCol129921_4341.
Organism-specific databases
EchoBASE	EB2398.
EcoGene	EG12505. ytfE.
Phylogenomic databases
eggNOG	COG2846.
HOGENOM	HOG000286324.
KO	K07322.
OMA	GNPINAM.
ProtClustDB	PRK10992.
Enzyme and pathway databases
BioCyc	EcoCyc:G7866-MONOMER. ECOL316407:JW4167-MONOMER.
Gene expression databases
Genevestigator	P69506.
Family and domain databases
HAMAP	MF_01606. RIC_YtfE.
InterPro	IPR007500. DUF542_ScdA_N. IPR023742. FeS-repair_YftE. IPR012312. Haemerythrin/HHE_cat-bd_motif. IPR019903. RIC_family. [Graphical view]
Pfam	PF01814. Hemerythrin. 1 hit. PF04405. ScdA_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR03652. FeS_repair_RIC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

YTFE_ECOLI

Accession

Primary (citable) accession number: P69506
Secondary accession number(s): P39313, Q2M696

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program