P69503 (APT_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenine phosphoribosyltransferase
Short name=APRT
EC=2.4.2.7

Gene names

Name:	apt
Ordered Locus Names:	b0469, JW0458

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. HAMAP MF_00004
Catalytic activity	AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00004
Pathway	Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1. HAMAP MF_00004
Subunit structure	Homodimer.
Subcellular location	Cytoplasm HAMAP MF_00004.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family.
Sequence caution	The sequence AAB40223.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Purine salvage
Cellular component	Cytoplasm
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	adenine salvage Inferred from direct assay. Source: EcoCyc purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: UniProtKB
Molecular function	adenine phosphoribosyltransferase activity Inferred from direct assay. Source: EcoCyc identical protein binding Inferred from direct assay. Source: EcoCyc magnesium ion binding Inferred from direct assay. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 183

183

Adenine phosphoribosyltransferase HAMAP MF_00004

PRO_0000149379

Secondary structure

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183

Helix Strand Turn

Details...

Helix

5 – 14

10

Beta strand

15 – 18

4

Beta strand

28 – 30

3

Helix

32 – 36

5

Helix

38 – 52

15

Turn

53 – 56

4

Beta strand

59 – 63

5

Helix

66 – 78

13

Beta strand

81 – 87

7

Beta strand

95 – 102

8

Beta strand

105 – 112

8

Helix

113 – 115

3

Beta strand

121 – 131

11

Helix

133 – 144

12

Beta strand

148 – 158

11

Helix

159 – 161

3

Helix

163 – 168

6

Turn

169 – 171

3

Beta strand

173 – 180

8

Sequences

	Sequence		Length	Mass (Da)
	P69503 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: AB45F0B5A219480D	FASTA	183	19,859
	10 20 30 40 50 60 MTATAQQLEY LKNSIKSIQD YPKPGILFRD VTSLLEDPKA YALSIDLLVE RYKNAGITKV 70 80 90 100 110 120 VGTEARGFLF GAPVALGLGV GFVPVRKPGK LPRETISETY DLEYGTDQLE IHVDAIKPGD 130 140 150 160 170 180 KVLVVDDLLA TGGTIEATVK LIRRLGGEVA DAAFIINLFD LGGEQRLEKQ GITSYSLVPF PGH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and deduced amino acid sequence of Escherichia coli adenine phosphoribosyltransferase and comparison with other analogous enzymes." Hershey H.V., Taylor M.W. Gene 43:287-293(1986) [PubMed: 3527873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Oligoribonuclease is encoded by a highly conserved gene in the 3'-5' exonuclease superfamily." Zhang X., Zhu L., Deutscher M.P. J. Bacteriol. 180:2779-2781(1998) [PubMed: 9573169] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-7.
[6]	"The adjacent dnaZ and dnaX genes of Escherichia coli are contained within one continuous open reading frame." Flower A.M., McHenry C.S. Nucleic Acids Res. 14:8091-8101(1986) [PubMed: 3534795] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 40-183.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M14040 Genomic DNA. Translation: AAA23455.1.
X04487 Genomic DNA. Translation: CAA28173.1.
U82664 Genomic DNA. Translation: AAB40223.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73571.1.
AP009048 Genomic DNA. Translation: BAE76248.1.
M38777 Genomic DNA. Translation: AAA23456.1.

PIR

RTECA. A25635.

RefSeq

NP_415002.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DY0	X-ray	1.25	A/B	1-183	[»]

ProteinModelPortal

P69503.

SMR

P69503. Positions 2-183.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-36165N.

IntAct

P69503. 7 interactions.

2D gel databases

SWISS-2DPAGE

P69503.

Proteomic databases

PRIDE

P69503.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000001461; EBESCP00000001461; EBESCG00000001216.
EBESCT00000015958; EBESCP00000015249; EBESCG00000015018.

GeneID

945113.

GenomeReviews

Gene locus JW0458 in contig AP009048_GR.
Gene locus b0469 in contig U00096_GR.

KEGG

ecj:JW0458.
eco:b0469.

PATRIC

32116095. VBIEscCol129921_0489.

Organism-specific databases

EchoBASE

EB0049.

EcoGene

EG10051. apt.

Phylogenomic databases

eggNOG

COG0503.

GeneTree

EBGT00050000010453.

HOGENOM

HBG703830.

OMA

GMEARGF.

PhylomeDB

P69503.

ProtClustDB

PRK02304.

Enzyme and pathway databases

BioCyc

EcoCyc:ADENPRIBOSYLTRAN-MONOMER.
MetaCyc:ADENPRIBOSYLTRAN-MONOMER.

Gene expression databases

Genevestigator

P69503.

Family and domain databases

HAMAP

MF_00004. Aden_phosphoribosyltr.
[Tree]

InterPro

IPR005764. Ade_phspho_trans.
IPR000836. PRibTrfase.
[Graphical view]

KO

K00759.

Pfam

PF00156. Pribosyltran. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01090. Apt. 1 hit.

PROSITE

PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

APT_ECOLI

Accession

Primary (citable) accession number: P69503
Secondary accession number(s): P07672 , P09993, P77121, Q2MBV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program