Adenine phosphoribosyltransferase - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P69503 (APT_ECOLI)

Last modified June 16, 2009. Version 41. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Adenine phosphoribosyltransferase
      Short name=APRT
    EC=2.4.2.7

Gene names

Name:	apt
Ordered Locus Names:	b0469, JW0458

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. HAMAP MF_00004
Catalytic activity	AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00004
Pathway	Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1. HAMAP MF_00004
Subunit structure	Homodimer. HAMAP MF_00004
Subcellular location	Cytoplasm. HAMAP MF_00004
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family.

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Ontologies

Keywords
Biological process	Purine salvage
Cellular component	Cytoplasm
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	adenine salvage Inferred from electronic annotation. Source: HAMAP purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: UniProtKB
Molecular function	adenine phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 183

183

Adenine phosphoribosyltransferase HAMAP MF_00004

PRO_0000149379

Secondary structure

183

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P69503-1 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: AB45F0B5A219480D
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FASTA

183

19,859

        10         20         30         40         50         60 
MTATAQQLEY LKNSIKSIQD YPKPGILFRD VTSLLEDPKA YALSIDLLVE RYKNAGITKV 

        70         80         90        100        110        120 
VGTEARGFLF GAPVALGLGV GFVPVRKPGK LPRETISETY DLEYGTDQLE IHVDAIKPGD 

       130        140        150        160        170        180 
KVLVVDDLLA TGGTIEATVK LIRRLGGEVA DAAFIINLFD LGGEQRLEKQ GITSYSLVPF 


PGH

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and deduced amino acid sequence of Escherichia coli adenine phosphoribosyltransferase and comparison with other analogous enzymes." Hershey H.V., Taylor M.W. Gene 43:287-293(1986) [PubMed: 3527873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Oligoribonuclease is encoded by a highly conserved gene in the 3'-5' exonuclease superfamily." Zhang X., Zhu L., Deutscher M.P. J. Bacteriol. 180:2779-2781(1998) [PubMed: 9573169] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-7.
[6]	"The adjacent dnaZ and dnaX genes of Escherichia coli are contained within one continuous open reading frame." Flower A.M., McHenry C.S. Nucleic Acids Res. 14:8091-8101(1986) [PubMed: 3534795] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 40-183.

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Cross-references

Sequence databases

M14040 Genomic DNA. Translation: AAA23455.1.
X04487 Genomic DNA. Translation: CAA28173.1.
U82664 Genomic DNA. Translation: AAB40223.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73571.1.
AP009048 Genomic DNA. Translation: BAE76248.1.
M38777 Genomic DNA. Translation: AAA23456.1.

PIR

RTECA. A25635.

RefSeq

AP_001118.1.
NP_415002.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DY0	X-ray	1.25	A/B	1-183	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P69503. 5 interactions.

2-D gel databases

SWISS-2DPAGE

P69503.

Genome annotation databases

GeneID

945113.

GenomeReviews

Gene locus JW0458 in contig AP009048_GR.
Gene locus b0469 in contig U00096_GR.

KEGG

ecj:JW0458.
eco:b0469.

Organism-specific databases

EchoBASE

EB0049.

EcoGene

EG10051. apt.

CMR

Search...

Phylogenomic databases

HOGENOM

P69503.

OMA

P69503. KPGKLPA.

Enzyme and pathway databases

BioCyc

EcoCyc:ADENPRIBOSYLTRAN-MON.
MetaCyc:ADENPRIBOSYLTRAN-MON.

Family and domain databases

HAMAP

MF_00004.
[Tree]

InterPro

IPR005764. Ade_phspho_trans.
IPR002375. Pr/py_Pribosyl_transf_CS.
IPR000836. PRibTrfase.
[Graphical view]

Pfam

PF00156. Pribosyltran. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01090. apt. 1 hit.

PROSITE

PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

APT_ECOLI

Accession

Primary (citable) accession number: P69503
Secondary accession number(s): P07672

Q2MBV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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