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Protein

Adenine phosphoribosyltransferase

Gene

apt

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.

Catalytic activityi

AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Pathwayi: AMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from adenine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Adenine phosphoribosyltransferase (apt)
This subpathway is part of the pathway AMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from adenine, the pathway AMP biosynthesis via salvage pathway and in Purine metabolism.

GO - Molecular functioni

  • adenine phosphoribosyltransferase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • magnesium ion binding Source: EcoCyc

GO - Biological processi

  • adenine salvage Source: EcoCyc
  • AMP salvage Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciEcoCyc:ADENPRIBOSYLTRAN-MONOMER.
ECOL316407:JW0458-MONOMER.
MetaCyc:ADENPRIBOSYLTRAN-MONOMER.
UniPathwayiUPA00588; UER00646.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine phosphoribosyltransferaseUniRule annotation (EC:2.4.2.7UniRule annotation)
Short name:
APRTUniRule annotation
Gene namesi
Name:aptUniRule annotation
Ordered Locus Names:b0469, JW0458
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10051. apt.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 183183Adenine phosphoribosyltransferasePRO_0000149379Add
BLAST

Proteomic databases

EPDiP69503.
PaxDbiP69503.
PRIDEiP69503.

2D gel databases

SWISS-2DPAGEP69503.

Interactioni

Subunit structurei

Homodimer.

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4261347. 8 interactions.
DIPiDIP-36165N.
IntActiP69503. 5 interactions.
STRINGi511145.b0469.

Structurei

Secondary structure

1
183
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 1410Combined sources
Beta strandi15 – 184Combined sources
Beta strandi28 – 303Combined sources
Helixi32 – 365Combined sources
Helixi38 – 5215Combined sources
Turni53 – 564Combined sources
Beta strandi59 – 635Combined sources
Helixi66 – 7813Combined sources
Beta strandi81 – 877Combined sources
Beta strandi95 – 1028Combined sources
Beta strandi105 – 1128Combined sources
Helixi113 – 1153Combined sources
Beta strandi121 – 13111Combined sources
Helixi133 – 14412Combined sources
Beta strandi148 – 15811Combined sources
Helixi159 – 1613Combined sources
Helixi163 – 1686Combined sources
Turni169 – 1713Combined sources
Beta strandi173 – 1808Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DY0X-ray1.25A/B1-183[»]
ProteinModelPortaliP69503.
SMRiP69503. Positions 2-183.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69503.

Family & Domainsi

Sequence similaritiesi

Belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4109003. Bacteria.
COG0503. LUCA.
HOGENOMiHOG000036776.
InParanoidiP69503.
KOiK00759.
OMAiATVEIHA.
OrthoDBiEOG6T4S55.
PhylomeDBiP69503.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_00004. Aden_phosphoribosyltr.
InterProiIPR005764. Ade_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01090. apt. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69503-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTATAQQLEY LKNSIKSIQD YPKPGILFRD VTSLLEDPKA YALSIDLLVE
60 70 80 90 100
RYKNAGITKV VGTEARGFLF GAPVALGLGV GFVPVRKPGK LPRETISETY
110 120 130 140 150
DLEYGTDQLE IHVDAIKPGD KVLVVDDLLA TGGTIEATVK LIRRLGGEVA
160 170 180
DAAFIINLFD LGGEQRLEKQ GITSYSLVPF PGH
Length:183
Mass (Da):19,859
Last modified:April 1, 1988 - v1
Checksum:iAB45F0B5A219480D
GO

Sequence cautioni

The sequence AAB40223.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14040 Genomic DNA. Translation: AAA23455.1.
X04487 Genomic DNA. Translation: CAA28173.1.
U82664 Genomic DNA. Translation: AAB40223.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73571.1.
AP009048 Genomic DNA. Translation: BAE76248.1.
M38777 Genomic DNA. Translation: AAA23456.1.
PIRiA25635. RTECA.
RefSeqiNP_415002.1. NC_000913.3.
WP_000127356.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73571; AAC73571; b0469.
BAE76248; BAE76248; BAE76248.
GeneIDi945113.
KEGGiecj:JW0458.
eco:b0469.
PATRICi32116095. VBIEscCol129921_0489.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14040 Genomic DNA. Translation: AAA23455.1.
X04487 Genomic DNA. Translation: CAA28173.1.
U82664 Genomic DNA. Translation: AAB40223.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73571.1.
AP009048 Genomic DNA. Translation: BAE76248.1.
M38777 Genomic DNA. Translation: AAA23456.1.
PIRiA25635. RTECA.
RefSeqiNP_415002.1. NC_000913.3.
WP_000127356.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DY0X-ray1.25A/B1-183[»]
ProteinModelPortaliP69503.
SMRiP69503. Positions 2-183.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261347. 8 interactions.
DIPiDIP-36165N.
IntActiP69503. 5 interactions.
STRINGi511145.b0469.

2D gel databases

SWISS-2DPAGEP69503.

Proteomic databases

EPDiP69503.
PaxDbiP69503.
PRIDEiP69503.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73571; AAC73571; b0469.
BAE76248; BAE76248; BAE76248.
GeneIDi945113.
KEGGiecj:JW0458.
eco:b0469.
PATRICi32116095. VBIEscCol129921_0489.

Organism-specific databases

EchoBASEiEB0049.
EcoGeneiEG10051. apt.

Phylogenomic databases

eggNOGiENOG4109003. Bacteria.
COG0503. LUCA.
HOGENOMiHOG000036776.
InParanoidiP69503.
KOiK00759.
OMAiATVEIHA.
OrthoDBiEOG6T4S55.
PhylomeDBiP69503.

Enzyme and pathway databases

UniPathwayiUPA00588; UER00646.
BioCyciEcoCyc:ADENPRIBOSYLTRAN-MONOMER.
ECOL316407:JW0458-MONOMER.
MetaCyc:ADENPRIBOSYLTRAN-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP69503.
PROiP69503.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_00004. Aden_phosphoribosyltr.
InterProiIPR005764. Ade_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01090. apt. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and deduced amino acid sequence of Escherichia coli adenine phosphoribosyltransferase and comparison with other analogous enzymes."
    Hershey H.V., Taylor M.W.
    Gene 43:287-293(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Oligoribonuclease is encoded by a highly conserved gene in the 3'-5' exonuclease superfamily."
    Zhang X., Zhu L., Deutscher M.P.
    J. Bacteriol. 180:2779-2781(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-7.
  6. "The adjacent dnaZ and dnaX genes of Escherichia coli are contained within one continuous open reading frame."
    Flower A.M., McHenry C.S.
    Nucleic Acids Res. 14:8091-8101(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 40-183.

Entry informationi

Entry nameiAPT_ECOLI
AccessioniPrimary (citable) accession number: P69503
Secondary accession number(s): P07672
, P09993, P77121, Q2MBV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: March 16, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.