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Protein

Adenine phosphoribosyltransferase

Gene

apt

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.

Catalytic activityi

AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Pathwayi: AMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from adenine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Adenine phosphoribosyltransferase (apt)
This subpathway is part of the pathway AMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from adenine, the pathway AMP biosynthesis via salvage pathway and in Purine metabolism.

GO - Molecular functioni

  • adenine phosphoribosyltransferase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • magnesium ion binding Source: EcoCyc

GO - Biological processi

  • adenine salvage Source: EcoCyc
  • AMP salvage Source: UniProtKB-UniPathway
  • purine ribonucleoside salvage Source: UniProtKB-KW

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine salvage

Enzyme and pathway databases

BioCyciEcoCyc:ADENPRIBOSYLTRAN-MONOMER
MetaCyc:ADENPRIBOSYLTRAN-MONOMER
SABIO-RKiP69503
UniPathwayiUPA00588; UER00646

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine phosphoribosyltransferaseUniRule annotation (EC:2.4.2.7UniRule annotation)
Short name:
APRTUniRule annotation
Gene namesi
Name:aptUniRule annotation
Ordered Locus Names:b0469, JW0458
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10051 apt

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001493791 – 183Adenine phosphoribosyltransferaseAdd BLAST183

Proteomic databases

EPDiP69503
PaxDbiP69503
PRIDEiP69503

2D gel databases

SWISS-2DPAGEiP69503

Interactioni

Subunit structurei

Homodimer.

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4261347, 8 interactors
DIPiDIP-36165N
IntActiP69503, 5 interactors
STRINGi316385.ECDH10B_0425

Structurei

Secondary structure

1183
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 14Combined sources10
Beta strandi15 – 18Combined sources4
Beta strandi28 – 30Combined sources3
Helixi32 – 36Combined sources5
Helixi38 – 52Combined sources15
Turni53 – 56Combined sources4
Beta strandi59 – 63Combined sources5
Helixi66 – 78Combined sources13
Beta strandi81 – 87Combined sources7
Beta strandi95 – 102Combined sources8
Beta strandi105 – 112Combined sources8
Helixi113 – 115Combined sources3
Beta strandi121 – 131Combined sources11
Helixi133 – 144Combined sources12
Beta strandi148 – 158Combined sources11
Helixi159 – 161Combined sources3
Helixi163 – 168Combined sources6
Turni169 – 171Combined sources3
Beta strandi173 – 180Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DY0X-ray1.25A/B1-183[»]
ProteinModelPortaliP69503
SMRiP69503
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69503

Family & Domainsi

Sequence similaritiesi

Belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4109003 Bacteria
COG0503 LUCA
HOGENOMiHOG000036776
InParanoidiP69503
KOiK00759
OMAiCAPIRKK
PhylomeDBiP69503

Family and domain databases

CDDicd06223 PRTases_typeI, 1 hit
HAMAPiMF_00004 Aden_phosphoribosyltr, 1 hit
InterProiView protein in InterPro
IPR005764 Ade_phspho_trans
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
PfamiView protein in Pfam
PF00156 Pribosyltran, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
TIGRFAMsiTIGR01090 apt, 1 hit
PROSITEiView protein in PROSITE
PS00103 PUR_PYR_PR_TRANSFER, 1 hit

Sequencei

Sequence statusi: Complete.

P69503-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTATAQQLEY LKNSIKSIQD YPKPGILFRD VTSLLEDPKA YALSIDLLVE
60 70 80 90 100
RYKNAGITKV VGTEARGFLF GAPVALGLGV GFVPVRKPGK LPRETISETY
110 120 130 140 150
DLEYGTDQLE IHVDAIKPGD KVLVVDDLLA TGGTIEATVK LIRRLGGEVA
160 170 180
DAAFIINLFD LGGEQRLEKQ GITSYSLVPF PGH
Length:183
Mass (Da):19,859
Last modified:April 1, 1988 - v1
Checksum:iAB45F0B5A219480D
GO

Sequence cautioni

The sequence AAB40223 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14040 Genomic DNA Translation: AAA23455.1
X04487 Genomic DNA Translation: CAA28173.1
U82664 Genomic DNA Translation: AAB40223.1 Different initiation.
U00096 Genomic DNA Translation: AAC73571.1
AP009048 Genomic DNA Translation: BAE76248.1
M38777 Genomic DNA Translation: AAA23456.1
PIRiA25635 RTECA
RefSeqiNP_415002.1, NC_000913.3
WP_000127356.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73571; AAC73571; b0469
BAE76248; BAE76248; BAE76248
GeneIDi945113
KEGGiecj:JW0458
eco:b0469
PATRICifig|1411691.4.peg.1807

Similar proteinsi

Entry informationi

Entry nameiAPT_ECOLI
AccessioniPrimary (citable) accession number: P69503
Secondary accession number(s): P07672
, P09993, P77121, Q2MBV8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: March 28, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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