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Reviewed, UniProtKB/Swiss-Prot P69503 (APT_ECOLI)

Last modified June 16, 2009. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Adenine phosphoribosyltransferase
      Short name=APRT
    EC=2.4.2.7
Gene names
Name: apt
Ordered Locus Names: b0469, JW0458
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length183 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. HAMAP MF_00004

Catalytic activity

AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00004

Pathway

Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1. HAMAP MF_00004

Subunit structure

Homodimer. HAMAP MF_00004

Subcellular location

Cytoplasm. HAMAP MF_00004

Sequence similarities

Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Ontologies

Keywords
   Biological processPurine salvage
   Cellular componentCytoplasm
   Molecular functionGlycosyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processadenine salvage

Inferred from electronic annotation. Source: HAMAP

purine ribonucleoside salvage

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytosol

Inferred from direct assay. Source: UniProtKB

   Molecular functionadenine phosphoribosyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 183183Adenine phosphoribosyltransferase HAMAP MF_00004
PRO_0000149379

Secondary structure

.................................. 183
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P69503-1 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: AB45F0B5A219480D

FASTA18319,859
        10         20         30         40         50         60 
MTATAQQLEY LKNSIKSIQD YPKPGILFRD VTSLLEDPKA YALSIDLLVE RYKNAGITKV 

        70         80         90        100        110        120 
VGTEARGFLF GAPVALGLGV GFVPVRKPGK LPRETISETY DLEYGTDQLE IHVDAIKPGD 

       130        140        150        160        170        180 
KVLVVDDLLA TGGTIEATVK LIRRLGGEVA DAAFIINLFD LGGEQRLEKQ GITSYSLVPF 


PGH 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence and deduced amino acid sequence of Escherichia coli adenine phosphoribosyltransferase and comparison with other analogous enzymes."
Hershey H.V., Taylor M.W.
Gene 43:287-293(1986) [PubMed: 3527873] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Oligoribonuclease is encoded by a highly conserved gene in the 3'-5' exonuclease superfamily."
Zhang X., Zhu L., Deutscher M.P.
J. Bacteriol. 180:2779-2781(1998) [PubMed: 9573169] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-7.
[6]"The adjacent dnaZ and dnaX genes of Escherichia coli are contained within one continuous open reading frame."
Flower A.M., McHenry C.S.
Nucleic Acids Res. 14:8091-8101(1986) [PubMed: 3534795] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 40-183.

Cross-references

Sequence databases

M14040 Genomic DNA. Translation: AAA23455.1.
X04487 Genomic DNA. Translation: CAA28173.1.
U82664 Genomic DNA. Translation: AAB40223.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73571.1.
AP009048 Genomic DNA. Translation: BAE76248.1.
M38777 Genomic DNA. Translation: AAA23456.1.
PIRRTECA. A25635.
RefSeqAP_001118.1.
NP_415002.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2DY0X-ray1.25A/B1-183[»]
ModBaseSearch...

Protein-protein interaction databases

IntActP69503. 5 interactions.

2-D gel databases

SWISS-2DPAGEP69503.

Genome annotation databases

GeneID945113.
GenomeReviewsGene locus JW0458 in contig AP009048_GR.
Gene locus b0469 in contig U00096_GR.
KEGGecj:JW0458.
eco:b0469.

Organism-specific databases

EchoBASEEB0049.
EcoGeneEG10051. apt.
CMRSearch...

Phylogenomic databases

HOGENOMP69503.
OMAP69503. KPGKLPA.

Enzyme and pathway databases

BioCycEcoCyc:ADENPRIBOSYLTRAN-MON.
MetaCyc:ADENPRIBOSYLTRAN-MON.

Family and domain databases

HAMAPMF_00004.
[Tree]
InterProIPR005764. Ade_phspho_trans.
IPR002375. Pr/py_Pribosyl_transf_CS.
IPR000836. PRibTrfase.
[Graphical view]
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
TIGRFAMsTIGR01090. apt. 1 hit.
PROSITEPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAPT_ECOLI
AccessionPrimary (citable) accession number: P69503
Secondary accession number(s): P07672 expand/collapse secondary AC list , P09993, P77121, Q2MBV8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents