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P69503 (APT_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Adenine phosphoribosyltransferase
Short name=APRT
EC=2.4.2.7
Gene names
Name:apt
Ordered Locus Names:b0469, JW0458
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length183 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. HAMAP-Rule MF_00004

Catalytic activity

AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_00004

Pathway

Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1. HAMAP-Rule MF_00004

Subunit structure

Homodimer.

Subcellular location

Cytoplasm HAMAP-Rule MF_00004.

Sequence similarities

Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Sequence caution

The sequence AAB40223.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 183183Adenine phosphoribosyltransferase HAMAP-Rule MF_00004
PRO_0000149379

Secondary structure

1 .................................. 183
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P69503 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: AB45F0B5A219480D

FASTA18319,859
        10         20         30         40         50         60 
MTATAQQLEY LKNSIKSIQD YPKPGILFRD VTSLLEDPKA YALSIDLLVE RYKNAGITKV 

        70         80         90        100        110        120 
VGTEARGFLF GAPVALGLGV GFVPVRKPGK LPRETISETY DLEYGTDQLE IHVDAIKPGD 

       130        140        150        160        170        180 
KVLVVDDLLA TGGTIEATVK LIRRLGGEVA DAAFIINLFD LGGEQRLEKQ GITSYSLVPF 


PGH

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence and deduced amino acid sequence of Escherichia coli adenine phosphoribosyltransferase and comparison with other analogous enzymes."
Hershey H.V., Taylor M.W.
Gene 43:287-293(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Oligoribonuclease is encoded by a highly conserved gene in the 3'-5' exonuclease superfamily."
Zhang X., Zhu L., Deutscher M.P.
J. Bacteriol. 180:2779-2781(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-7.
[6]"The adjacent dnaZ and dnaX genes of Escherichia coli are contained within one continuous open reading frame."
Flower A.M., McHenry C.S.
Nucleic Acids Res. 14:8091-8101(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 40-183.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M14040 Genomic DNA. Translation: AAA23455.1.
X04487 Genomic DNA. Translation: CAA28173.1.
U82664 Genomic DNA. Translation: AAB40223.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73571.1.
AP009048 Genomic DNA. Translation: BAE76248.1.
M38777 Genomic DNA. Translation: AAA23456.1.
PIRRTECA. A25635.
RefSeqNP_415002.1. NC_000913.2.
YP_488760.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2DY0X-ray1.25A/B1-183[»]
ProteinModelPortalP69503.
SMRP69503. Positions 2-183.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-36165N.
IntActP69503. 5 interactions.
STRING511145.b0469.

2D gel databases

SWISS-2DPAGEP69503.

Proteomic databases

PaxDbP69503.
PRIDEP69503.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73571; AAC73571; b0469.
BAE76248; BAE76248; BAE76248.
GeneID12932353.
945113.
KEGGecj:Y75_p0456.
eco:b0469.
PATRIC32116095. VBIEscCol129921_0489.

Organism-specific databases

EchoBASEEB0049.
EcoGeneEG10051. apt.

Phylogenomic databases

eggNOGCOG0503.
HOGENOMHOG000036776.
KOK00759.
OMADYALEYG.
ProtClustDBPRK02304.

Enzyme and pathway databases

BioCycEcoCyc:ADENPRIBOSYLTRAN-MONOMER.
ECOL316407:JW0458-MONOMER.
MetaCyc:ADENPRIBOSYLTRAN-MONOMER.
UniPathwayUPA00588; UER00646.

Gene expression databases

GenevestigatorP69503.

Family and domain databases

HAMAPMF_00004. Aden_phosphoribosyltr.
InterProIPR005764. Ade_phspho_trans.
IPR000836. PRibTrfase_dom.
[Graphical view]
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
TIGRFAMsTIGR01090. apt. 1 hit.
PROSITEPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP69503.

Entry information

Entry nameAPT_ECOLI
AccessionPrimary (citable) accession number: P69503
Secondary accession number(s): P07672 expand/collapse secondary AC list , P09993, P77121, Q2MBV8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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