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Protein

Cytochrome c-type biogenesis protein CcmE

Gene

ccmE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei130Heme (covalent; via pros nitrogen)1
Metal bindingi134Iron (heme axial ligand)1

GO - Molecular functioni

  • heme binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cytochrome c-heme linkage Source: EcoCyc
  • heme transport Source: EcoCyc

Keywordsi

Biological processCytochrome c-type biogenesis
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:CCME-MONOMER
MetaCyc:CCME-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c-type biogenesis protein CcmEUniRule annotation
Alternative name(s):
Cytochrome c maturation protein EUniRule annotation
Heme chaperone CcmEUniRule annotation
Gene namesi
Name:ccmEUniRule annotation
Synonyms:yejS
Ordered Locus Names:b2197, JW2185
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12055 ccmE

Subcellular locationi

  • Cell inner membrane UniRule annotation; Single-pass type II membrane protein UniRule annotation; Periplasmic side UniRule annotation
  • Note: Stabilized by CcmD in the membrane.

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicUniRule annotation8
Transmembranei9 – 29Helical; Signal-anchor for type II membrane proteinUniRule annotationAdd BLAST21
Topological domaini30 – 159PeriplasmicUniRule annotationAdd BLAST130

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi130H → A: Abolishes heme binding. 1 Publication1
Mutagenesisi130H → C: Can still form a covalent bond with heme, but blocks heme release transfer to cytochrome c. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002015751 – 159Cytochrome c-type biogenesis protein CcmEAdd BLAST159

Proteomic databases

PaxDbiP69490
PRIDEiP69490

Interactioni

Subunit structurei

Forms a ternary complex with CcmC and CcmD. Interacts with CcmF. Shuttles between CcmC and CcmF for heme delivery.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ccmCP0ABM16EBI-1128007,EBI-2123469

Protein-protein interaction databases

BioGridi4260483, 134 interactors
DIPiDIP-9255N
IntActiP69490, 6 interactors
MINTiP69490
STRINGi316385.ECDH10B_2354

Structurei

Secondary structure

1159
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi33 – 35Combined sources3
Turni40 – 44Combined sources5
Turni48 – 50Combined sources3
Beta strandi58 – 66Combined sources9
Turni68 – 70Combined sources3
Beta strandi75 – 85Combined sources11
Beta strandi90 – 97Combined sources8
Beta strandi107 – 115Combined sources9
Beta strandi117 – 125Combined sources9
Helixi137 – 140Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SR3NMR-A30-159[»]
ProteinModelPortaliP69490
SMRiP69490
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69490

Family & Domainsi

Sequence similaritiesi

Belongs to the CcmE/CycJ family.UniRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108ZKK Bacteria
COG2332 LUCA
HOGENOMiHOG000009661
InParanoidiP69490
KOiK02197
OMAiHVEFAVH
PhylomeDBiP69490

Family and domain databases

HAMAPiMF_01959 CcmE, 1 hit
InterProiView protein in InterPro
IPR004329 CcmE
IPR036127 CcmE-like_sf
PANTHERiPTHR34128 PTHR34128, 1 hit
PfamiView protein in Pfam
PF03100 CcmE, 1 hit
SUPFAMiSSF82093 SSF82093, 1 hit

Sequencei

Sequence statusi: Complete.

P69490-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIRRKNRLW IACAVLAGLA LTIGLVLYAL RSNIDLFYTP GEILYGKRET
60 70 80 90 100
QQMPEVGQRL RVGGMVMPGS VQRDPNSLKV TFTIYDAEGS VDVSYEGILP
110 120 130 140 150
DLFREGQGVV VQGELEKGNH ILAKEVLAKH DENYTPPEVE KAMEANHRRP

ASVYKDPAS
Length:159
Mass (Da):17,698
Last modified:March 15, 2005 - v1
Checksum:iFA0585B8CD6446FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00008 Genomic DNA Translation: AAA16389.1
U00096 Genomic DNA Translation: AAC75257.1
AP009048 Genomic DNA Translation: BAE76660.1
PIRiC64989
RefSeqiNP_416701.1, NC_000913.3
WP_001026418.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75257; AAC75257; b2197
BAE76660; BAE76660; BAE76660
GeneIDi946697
KEGGiecj:JW2185
eco:b2197
PATRICifig|1411691.4.peg.39

Similar proteinsi

Entry informationi

Entry nameiCCME_ECOLI
AccessioniPrimary (citable) accession number: P69490
Secondary accession number(s): P33928, Q2MAP6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: March 28, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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